Knoellia remsis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Knoellia

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3374 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0UQU7|A0A2T0UQU7_9MICO Amino acid ABC transporter membrane protein (PAAT family) OS=Knoellia remsis OX=407159 GN=BCF74_10791 PE=3 SV=1
MM1 pKa = 7.51RR2 pKa = 11.84RR3 pKa = 11.84SFLAGLAGLALALTTLVGASPAGAINSYY31 pKa = 9.57NAEE34 pKa = 4.14PAPEE38 pKa = 3.97RR39 pKa = 11.84TEE41 pKa = 4.04VGALVGFYY49 pKa = 10.83DD50 pKa = 4.81RR51 pKa = 11.84DD52 pKa = 3.37GDD54 pKa = 4.1GYY56 pKa = 11.17LDD58 pKa = 3.53RR59 pKa = 11.84FRR61 pKa = 11.84WYY63 pKa = 9.26CTGTMVDD70 pKa = 4.91DD71 pKa = 4.29DD72 pKa = 4.52TFLTAAHH79 pKa = 6.29CTDD82 pKa = 3.87GGLPDD87 pKa = 4.27DD88 pKa = 4.77RR89 pKa = 11.84YY90 pKa = 10.2FVSLEE95 pKa = 3.76EE96 pKa = 4.56DD97 pKa = 3.63FQGLLDD103 pKa = 5.53AYY105 pKa = 10.07DD106 pKa = 4.16KK107 pKa = 11.55EE108 pKa = 4.41NGPVPTDD115 pKa = 3.4PAEE118 pKa = 4.33LEE120 pKa = 4.27TYY122 pKa = 10.57LATKK126 pKa = 9.09VQYY129 pKa = 10.57FLDD132 pKa = 3.55QGWIVEE138 pKa = 4.46GTPHH142 pKa = 6.91QDD144 pKa = 2.87PEE146 pKa = 4.54YY147 pKa = 9.46PGNGSDD153 pKa = 3.68SQDD156 pKa = 2.82IAVIDD161 pKa = 4.03FAQWDD166 pKa = 4.16TTPQDD171 pKa = 3.08VWDD174 pKa = 4.92FTPATLPTLGQLDD187 pKa = 4.16EE188 pKa = 4.89LGSRR192 pKa = 11.84ALDD195 pKa = 3.78AADD198 pKa = 3.08WWVVGYY204 pKa = 7.56GTSEE208 pKa = 4.09AVRR211 pKa = 11.84GPGGQTHH218 pKa = 7.19PGGGVRR224 pKa = 11.84MKK226 pKa = 11.13ALVDD230 pKa = 4.54FNALNPTWVRR240 pKa = 11.84LGMIAARR247 pKa = 11.84GYY249 pKa = 11.08GGACYY254 pKa = 10.24GDD256 pKa = 3.53SGGPNYY262 pKa = 10.34VEE264 pKa = 4.83IDD266 pKa = 3.65GQLVLAATTITGDD279 pKa = 3.91GPCYY283 pKa = 9.17ATNVVYY289 pKa = 10.67RR290 pKa = 11.84LDD292 pKa = 3.65TEE294 pKa = 4.47GARR297 pKa = 11.84EE298 pKa = 3.85FLAPYY303 pKa = 10.37VDD305 pKa = 4.75LPP307 pKa = 3.95

Molecular weight: 33.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0UN72|A0A2T0UN72_9MICO NADH-quinone oxidoreductase subunit B OS=Knoellia remsis OX=407159 GN=nuoB PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3374

0

3374

1082194

24

2174

320.7

34.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.076 ± 0.057

0.621 ± 0.01

6.536 ± 0.039

5.765 ± 0.048

2.741 ± 0.03

9.319 ± 0.04

2.04 ± 0.021

3.592 ± 0.03

2.169 ± 0.034

9.745 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.854 ± 0.016

1.837 ± 0.026

5.654 ± 0.032

2.742 ± 0.022

7.604 ± 0.053

5.496 ± 0.032

6.499 ± 0.042

9.291 ± 0.043

1.573 ± 0.02

1.847 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski