Paracoccus sp. JM45

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Paracoccus; unclassified Paracoccus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3379 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A3DU69|A0A3A3DU69_9RHOB IS6 family transposase (Fragment) OS=Paracoccus sp. JM45 OX=2283626 GN=DWB67_16910 PE=4 SV=1
MM1 pKa = 7.58VSTAQLNIVNLQVEE15 pKa = 4.6AAGQIAIDD23 pKa = 4.07KK24 pKa = 11.04ANGSYY29 pKa = 10.4AANLAANDD37 pKa = 5.0KK38 pKa = 10.38YY39 pKa = 11.13YY40 pKa = 11.03LWQSDD45 pKa = 4.09FQTLPTSMFGEE56 pKa = 4.32PEE58 pKa = 4.09LYY60 pKa = 10.47AEE62 pKa = 4.8RR63 pKa = 11.84LHH65 pKa = 7.27DD66 pKa = 4.76ALPGVTTLRR75 pKa = 11.84IPFNLNSFNADD86 pKa = 3.07GTLHH90 pKa = 7.08PDD92 pKa = 3.66FEE94 pKa = 5.09RR95 pKa = 11.84FLVAAANEE103 pKa = 4.31GFTFIMVQMEE113 pKa = 4.57GAAQTLTASGPNALDD128 pKa = 3.22QMRR131 pKa = 11.84DD132 pKa = 3.45GLSVEE137 pKa = 4.45VYY139 pKa = 11.15DD140 pKa = 5.98RR141 pKa = 11.84MEE143 pKa = 4.18QGWTMLLDD151 pKa = 3.47WMDD154 pKa = 3.36THH156 pKa = 7.1PSVKK160 pKa = 10.21DD161 pKa = 3.01SVYY164 pKa = 9.78AHH166 pKa = 5.99EE167 pKa = 4.83VVNEE171 pKa = 3.61PAAYY175 pKa = 7.98NTATFYY181 pKa = 9.73GTSRR185 pKa = 11.84PAALQDD191 pKa = 3.91FVSLYY196 pKa = 8.23ATHH199 pKa = 6.66MVQLGQMIQDD209 pKa = 3.58RR210 pKa = 11.84ADD212 pKa = 3.27GTKK215 pKa = 10.34IMVGGWNYY223 pKa = 10.66SAQFQQLADD232 pKa = 3.18ISMGGGSALDD242 pKa = 3.97YY243 pKa = 10.78IRR245 pKa = 11.84EE246 pKa = 4.16GLGDD250 pKa = 3.79SLVWSAHH257 pKa = 6.82LYY259 pKa = 9.78PGWLGTGGMSDD270 pKa = 3.61PNAIRR275 pKa = 11.84TVLDD279 pKa = 4.37EE280 pKa = 4.68IYY282 pKa = 10.97SSIVDD287 pKa = 3.66DD288 pKa = 4.69SLILSEE294 pKa = 4.85TNAQGNEE301 pKa = 3.77AYY303 pKa = 10.72NLFSDD308 pKa = 3.9RR309 pKa = 11.84PEE311 pKa = 4.02VQGFTQVYY319 pKa = 9.97DD320 pKa = 3.31WFADD324 pKa = 3.68RR325 pKa = 11.84GIGISWFTGSQYY337 pKa = 11.15GGSVLSRR344 pKa = 11.84MDD346 pKa = 3.76PDD348 pKa = 3.6GTLSFVQQGSFGAAMDD364 pKa = 4.25AFTLGGEE371 pKa = 4.57DD372 pKa = 3.99PDD374 pKa = 4.08HH375 pKa = 7.05AGGEE379 pKa = 4.38VVEE382 pKa = 4.34IQLIRR387 pKa = 11.84GRR389 pKa = 11.84MRR391 pKa = 11.84NQTTDD396 pKa = 3.38PDD398 pKa = 3.73YY399 pKa = 10.88QAYY402 pKa = 10.83NEE404 pKa = 3.94MDD406 pKa = 2.9IAQFLGSGLGHH417 pKa = 7.11GGNDD421 pKa = 3.57TLTGSAVANNFLYY434 pKa = 10.76GGTSNDD440 pKa = 3.4LVTGNILDD448 pKa = 4.24DD449 pKa = 3.95FLYY452 pKa = 10.75GQDD455 pKa = 4.88DD456 pKa = 4.22NDD458 pKa = 4.6TIDD461 pKa = 4.24GGLSGHH467 pKa = 6.34DD468 pKa = 3.46HH469 pKa = 6.75LFGGGGEE476 pKa = 4.2DD477 pKa = 5.38LIIGGAGISQMYY489 pKa = 9.74GGTGGDD495 pKa = 3.27TFVAHH500 pKa = 6.8PRR502 pKa = 11.84GQTIIVDD509 pKa = 3.65YY510 pKa = 10.92DD511 pKa = 3.64STEE514 pKa = 3.9GDD516 pKa = 3.22QLIINEE522 pKa = 4.21ADD524 pKa = 3.53FTVEE528 pKa = 3.98DD529 pKa = 3.87VRR531 pKa = 11.84RR532 pKa = 11.84LAQSLDD538 pKa = 2.82WDD540 pKa = 4.48LDD542 pKa = 3.53DD543 pKa = 6.52SRR545 pKa = 11.84DD546 pKa = 3.98LRR548 pKa = 11.84IALPGGGEE556 pKa = 4.09IIMLGMGDD564 pKa = 3.57RR565 pKa = 11.84LEE567 pKa = 5.04DD568 pKa = 3.66VIASLFPAQGNVEE581 pKa = 4.18PAAPAPFHH589 pKa = 6.8LLLQGRR595 pKa = 11.84PGVDD599 pKa = 2.74ILAGGAGNDD608 pKa = 4.01TIYY611 pKa = 11.0GDD613 pKa = 3.62PEE615 pKa = 4.24GAVGADD621 pKa = 3.37QLYY624 pKa = 10.92GGANRR629 pKa = 11.84DD630 pKa = 3.79LIYY633 pKa = 11.0GGGEE637 pKa = 3.77ADD639 pKa = 4.44YY640 pKa = 11.16IDD642 pKa = 5.13GGTGNDD648 pKa = 3.31TLYY651 pKa = 11.11GGEE654 pKa = 4.52GGDD657 pKa = 4.06AIHH660 pKa = 7.1GGLHH664 pKa = 6.21NDD666 pKa = 3.12VLEE669 pKa = 4.78GGGGDD674 pKa = 3.82DD675 pKa = 4.13TVHH678 pKa = 6.61GSNGQDD684 pKa = 3.34VLSGGLGSDD693 pKa = 3.59TLSGDD698 pKa = 3.51GGRR701 pKa = 11.84DD702 pKa = 3.12TLYY705 pKa = 11.23GGDD708 pKa = 4.4SSDD711 pKa = 4.04LLLGGGGNDD720 pKa = 4.02MIFGGNGNDD729 pKa = 3.31TLYY732 pKa = 11.26GGNRR736 pKa = 11.84NDD738 pKa = 4.83LLYY741 pKa = 11.15GDD743 pKa = 5.01ASNDD747 pKa = 3.29VLYY750 pKa = 11.03GEE752 pKa = 4.74KK753 pKa = 10.2WNDD756 pKa = 3.26TLYY759 pKa = 11.33GGAGQDD765 pKa = 3.53DD766 pKa = 5.51LYY768 pKa = 11.52GGTEE772 pKa = 4.1DD773 pKa = 5.23DD774 pKa = 5.27DD775 pKa = 5.95LYY777 pKa = 11.71GEE779 pKa = 4.52NSNDD783 pKa = 3.66TLDD786 pKa = 4.12GGSGNDD792 pKa = 3.45MLDD795 pKa = 3.31GGGQNDD801 pKa = 4.64LLYY804 pKa = 11.01GGTGNDD810 pKa = 3.98DD811 pKa = 3.38LHH813 pKa = 8.58GGFGLDD819 pKa = 3.08TLYY822 pKa = 11.41GEE824 pKa = 5.33NGDD827 pKa = 3.96EE828 pKa = 4.17LLNGGGMADD837 pKa = 4.06MIYY840 pKa = 10.56GGSGNDD846 pKa = 3.51TLLGEE851 pKa = 5.13AGNDD855 pKa = 3.97WIHH858 pKa = 6.6GGPGQDD864 pKa = 3.1VATGGGGADD873 pKa = 3.1TFVFDD878 pKa = 5.61LGDD881 pKa = 3.68GSLRR885 pKa = 11.84ITDD888 pKa = 4.14FSVNQGDD895 pKa = 4.41ILRR898 pKa = 11.84LDD900 pKa = 3.53AALLDD905 pKa = 4.37GAPSVAALQEE915 pKa = 4.31RR916 pKa = 11.84ATLTDD921 pKa = 3.57AGTSIIFTDD930 pKa = 3.99GNTVLIANFQGTFSDD945 pKa = 3.75SFVDD949 pKa = 3.79ILL951 pKa = 4.53

Molecular weight: 99.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A3DD12|A0A3A3DD12_9RHOB ABC transporter substrate-binding protein OS=Paracoccus sp. JM45 OX=2283626 GN=DWB67_11230 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.99GGRR28 pKa = 11.84RR29 pKa = 11.84ILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.91SLSAA44 pKa = 3.86

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3379

0

3379

1057785

26

1512

313.0

34.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.094 ± 0.055

0.867 ± 0.011

6.442 ± 0.04

5.022 ± 0.031

3.644 ± 0.026

8.552 ± 0.043

2.111 ± 0.022

5.506 ± 0.034

2.967 ± 0.029

10.098 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.032 ± 0.022

2.69 ± 0.023

5.126 ± 0.03

3.664 ± 0.022

6.709 ± 0.045

5.254 ± 0.029

5.594 ± 0.026

7.076 ± 0.034

1.442 ± 0.018

2.111 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski