Rhodotorula sp. JG-1b
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6678 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A109FG84|A0A109FG84_9BASI Uncharacterized protein OS=Rhodotorula sp. JG-1b OX=1305733 GN=RHOSPDRAFT_34523 PE=4 SV=1
MM1 pKa = 7.13 LQSAPSGADD10 pKa = 2.95 NDD12 pKa = 4.02 YY13 pKa = 11.62 DD14 pKa = 4.45 DD15 pKa = 6.26 LYY17 pKa = 11.54 GDD19 pKa = 5.03 LYY21 pKa = 10.46 PAEE24 pKa = 4.47 TEE26 pKa = 4.07 ATPAASTSAAPSASATAPAAASPAGSTSTAAATGAPAATGYY67 pKa = 10.86 GGDD70 pKa = 3.75 AARR73 pKa = 11.84 PAQLPQVPTGAAMPAAGGMVHH94 pKa = 7.6 DD95 pKa = 5.07 IGIPRR100 pKa = 11.84 PQALQANALASKK112 pKa = 8.94 TADD115 pKa = 3.35 QLDD118 pKa = 3.82 EE119 pKa = 4.28 GG120 pKa = 4.45
Molecular weight: 11.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.77
IPC_protein 3.706
Toseland 3.49
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.834
Patrickios 1.888
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.758
Protein with the highest isoelectric point:
>tr|A0A109FAM0|A0A109FAM0_9BASI Uncharacterized protein (Fragment) OS=Rhodotorula sp. JG-1b OX=1305733 GN=RHOSPDRAFT_27953 PE=4 SV=1
MM1 pKa = 6.48 THH3 pKa = 5.52 EE4 pKa = 4.97 AVWFSHH10 pKa = 4.79 PRR12 pKa = 11.84 KK13 pKa = 9.21 YY14 pKa = 11.07 GKK16 pKa = 10.25 GSRR19 pKa = 11.84 GCRR22 pKa = 11.84 ICQHH26 pKa = 4.81 QAGLIRR32 pKa = 11.84 KK33 pKa = 8.7 YY34 pKa = 10.83 GLNICRR40 pKa = 11.84 QCFRR44 pKa = 11.84 EE45 pKa = 3.94 RR46 pKa = 11.84 SDD48 pKa = 5.08 AIGFTKK54 pKa = 10.49 NRR56 pKa = 3.76
Molecular weight: 6.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.545
IPC_protein 10.087
Toseland 10.467
ProMoST 10.452
Dawson 10.599
Bjellqvist 10.306
Wikipedia 10.774
Rodwell 10.877
Grimsley 10.643
Solomon 10.672
Lehninger 10.657
Nozaki 10.526
DTASelect 10.277
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.54
Patrickios 10.672
IPC_peptide 10.672
IPC2_peptide 9.677
IPC2.peptide.svr19 8.53
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6678
0
6678
3457298
50
5368
517.7
56.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.128 ± 0.035
1.015 ± 0.011
5.688 ± 0.024
6.156 ± 0.031
3.305 ± 0.016
7.158 ± 0.027
2.287 ± 0.013
3.737 ± 0.021
4.139 ± 0.029
9.291 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.68 ± 0.009
2.688 ± 0.012
6.809 ± 0.041
3.781 ± 0.023
6.822 ± 0.024
8.621 ± 0.043
5.88 ± 0.02
6.226 ± 0.024
1.274 ± 0.01
2.313 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here