Amino acid dipepetide frequency for Rhodotorula sp. JG-1b

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.27AlaAla: 16.27 ± 0.145	1.088AlaCys: 1.088 ± 0.02	5.449AlaAsp: 5.449 ± 0.049	6.322AlaGlu: 6.322 ± 0.054	3.531AlaPhe: 3.531 ± 0.034	7.732AlaGly: 7.732 ± 0.058	2.289AlaHis: 2.289 ± 0.029	3.912AlaIle: 3.912 ± 0.034	4.377AlaLys: 4.377 ± 0.048	9.786AlaLeu: 9.786 ± 0.071	1.847AlaMet: 1.847 ± 0.025	2.925AlaAsn: 2.925 ± 0.034	7.415AlaPro: 7.415 ± 0.08	4.18AlaGln: 4.18 ± 0.041	7.028AlaArg: 7.028 ± 0.055	10.121AlaSer: 10.121 ± 0.081	6.961AlaThr: 6.961 ± 0.056	6.522AlaVal: 6.522 ± 0.044	1.239AlaTrp: 1.239 ± 0.021	2.291AlaTyr: 2.291 ± 0.026	0.0AlaXaa: 0.0 ± 0.0
Cys	0.935CysAla: 0.935 ± 0.018	0.191CysCys: 0.191 ± 0.008	0.553CysAsp: 0.553 ± 0.013	0.529CysGlu: 0.529 ± 0.013	0.421CysPhe: 0.421 ± 0.012	0.845CysGly: 0.845 ± 0.018	0.238CysHis: 0.238 ± 0.01	0.48CysIle: 0.48 ± 0.012	0.397CysLys: 0.397 ± 0.01	1.047CysLeu: 1.047 ± 0.019	0.192CysMet: 0.192 ± 0.008	0.261CysAsn: 0.261 ± 0.011	0.592CysPro: 0.592 ± 0.016	0.34CysGln: 0.34 ± 0.01	0.664CysArg: 0.664 ± 0.014	0.77CysSer: 0.77 ± 0.017	0.601CysThr: 0.601 ± 0.015	0.666CysVal: 0.666 ± 0.016	0.163CysTrp: 0.163 ± 0.006	0.269CysTyr: 0.269 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	5.915AspAla: 5.915 ± 0.049	0.51AspCys: 0.51 ± 0.013	5.136AspAsp: 5.136 ± 0.076	5.124AspGlu: 5.124 ± 0.058	1.99AspPhe: 1.99 ± 0.025	4.164AspGly: 4.164 ± 0.044	1.169AspHis: 1.169 ± 0.021	1.903AspIle: 1.903 ± 0.026	2.181AspLys: 2.181 ± 0.028	5.477AspLeu: 5.477 ± 0.051	0.896AspMet: 0.896 ± 0.017	1.273AspAsn: 1.273 ± 0.019	3.717AspPro: 3.717 ± 0.037	1.809AspGln: 1.809 ± 0.021	3.384AspArg: 3.384 ± 0.037	4.134AspSer: 4.134 ± 0.035	2.29AspThr: 2.29 ± 0.025	3.588AspVal: 3.588 ± 0.037	0.855AspTrp: 0.855 ± 0.017	1.36AspTyr: 1.36 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	6.458GluAla: 6.458 ± 0.05	0.524GluCys: 0.524 ± 0.014	4.005GluAsp: 4.005 ± 0.042	5.904GluGlu: 5.904 ± 0.08	1.58GluPhe: 1.58 ± 0.023	4.148GluGly: 4.148 ± 0.036	1.394GluHis: 1.394 ± 0.022	2.462GluIle: 2.462 ± 0.024	2.94GluLys: 2.94 ± 0.037	5.551GluLeu: 5.551 ± 0.048	1.309GluMet: 1.309 ± 0.021	1.555GluAsn: 1.555 ± 0.021	2.972GluPro: 2.972 ± 0.033	2.772GluGln: 2.772 ± 0.028	4.836GluArg: 4.836 ± 0.048	3.766GluSer: 3.766 ± 0.038	3.412GluThr: 3.412 ± 0.035	3.673GluVal: 3.673 ± 0.041	0.923GluTrp: 0.923 ± 0.018	1.374GluTyr: 1.374 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.651PheAla: 3.651 ± 0.034	0.462PheCys: 0.462 ± 0.011	2.215PheAsp: 2.215 ± 0.027	2.01PheGlu: 2.01 ± 0.028	1.378PhePhe: 1.378 ± 0.025	2.692PheGly: 2.692 ± 0.038	0.724PheHis: 0.724 ± 0.015	1.233PheIle: 1.233 ± 0.021	1.176PheLys: 1.176 ± 0.021	3.196PheLeu: 3.196 ± 0.035	0.516PheMet: 0.516 ± 0.014	0.909PheAsn: 0.909 ± 0.016	1.805PhePro: 1.805 ± 0.025	1.016PheGln: 1.016 ± 0.017	1.918PheArg: 1.918 ± 0.025	2.709PheSer: 2.709 ± 0.03	1.694PheThr: 1.694 ± 0.023	2.413PheVal: 2.413 ± 0.027	0.473PheTrp: 0.473 ± 0.012	0.857PheTyr: 0.857 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	7.098GlyAla: 7.098 ± 0.059	0.714GlyCys: 0.714 ± 0.018	3.768GlyAsp: 3.768 ± 0.037	4.051GlyGlu: 4.051 ± 0.038	2.472GlyPhe: 2.472 ± 0.035	8.505GlyGly: 8.505 ± 0.098	1.554GlyHis: 1.554 ± 0.022	2.775GlyIle: 2.775 ± 0.031	3.442GlyLys: 3.442 ± 0.042	5.897GlyLeu: 5.897 ± 0.049	1.374GlyMet: 1.374 ± 0.024	1.918GlyAsn: 1.918 ± 0.023	3.728GlyPro: 3.728 ± 0.038	2.522GlyGln: 2.522 ± 0.031	4.517GlyArg: 4.517 ± 0.044	5.965GlySer: 5.965 ± 0.055	4.128GlyThr: 4.128 ± 0.041	4.293GlyVal: 4.293 ± 0.044	1.092GlyTrp: 1.092 ± 0.019	1.769GlyTyr: 1.769 ± 0.026	0.0GlyXaa: 0.0 ± 0.0
His	2.44HisAla: 2.44 ± 0.032	0.255HisCys: 0.255 ± 0.01	1.309HisAsp: 1.309 ± 0.022	1.262HisGlu: 1.262 ± 0.018	0.869HisPhe: 0.869 ± 0.016	1.587HisGly: 1.587 ± 0.025	0.921HisHis: 0.921 ± 0.024	0.797HisIle: 0.797 ± 0.014	0.713HisLys: 0.713 ± 0.013	2.529HisLeu: 2.529 ± 0.029	0.327HisMet: 0.327 ± 0.011	0.557HisAsn: 0.557 ± 0.013	1.758HisPro: 1.758 ± 0.03	0.849HisGln: 0.849 ± 0.016	1.554HisArg: 1.554 ± 0.024	1.726HisSer: 1.726 ± 0.025	1.055HisThr: 1.055 ± 0.017	1.485HisVal: 1.485 ± 0.023	0.28HisTrp: 0.28 ± 0.009	0.599HisTyr: 0.599 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.026IleAla: 4.026 ± 0.039	0.511IleCys: 0.511 ± 0.014	2.378IleAsp: 2.378 ± 0.026	2.437IleGlu: 2.437 ± 0.027	1.337IlePhe: 1.337 ± 0.028	2.63IleGly: 2.63 ± 0.034	0.792IleHis: 0.792 ± 0.016	1.436IleIle: 1.436 ± 0.026	1.662IleLys: 1.662 ± 0.024	3.598IleLeu: 3.598 ± 0.036	0.635IleMet: 0.635 ± 0.016	1.106IleAsn: 1.106 ± 0.019	2.311IlePro: 2.311 ± 0.026	1.228IleGln: 1.228 ± 0.022	2.444IleArg: 2.444 ± 0.03	2.776IleSer: 2.776 ± 0.03	1.895IleThr: 1.895 ± 0.028	2.753IleVal: 2.753 ± 0.032	0.492IleTrp: 0.492 ± 0.013	0.926IleTyr: 0.926 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	4.351LysAla: 4.351 ± 0.047	0.34LysCys: 0.34 ± 0.009	2.147LysAsp: 2.147 ± 0.029	2.854LysGlu: 2.854 ± 0.035	1.028LysPhe: 1.028 ± 0.018	2.865LysGly: 2.865 ± 0.032	0.917LysHis: 0.917 ± 0.017	1.606LysIle: 1.606 ± 0.025	2.848LysLys: 2.848 ± 0.049	3.599LysLeu: 3.599 ± 0.039	0.771LysMet: 0.771 ± 0.016	1.146LysAsn: 1.146 ± 0.02	2.367LysPro: 2.367 ± 0.03	1.7LysGln: 1.7 ± 0.029	3.602LysArg: 3.602 ± 0.038	2.814LysSer: 2.814 ± 0.033	2.283LysThr: 2.283 ± 0.032	2.624LysVal: 2.624 ± 0.034	0.546LysTrp: 0.546 ± 0.013	0.978LysTyr: 0.978 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	10.715LeuAla: 10.715 ± 0.069	1.09LeuCys: 1.09 ± 0.018	5.634LeuAsp: 5.634 ± 0.044	5.89LeuGlu: 5.89 ± 0.049	3.188LeuPhe: 3.188 ± 0.032	6.009LeuGly: 6.009 ± 0.049	2.243LeuHis: 2.243 ± 0.028	3.194LeuIle: 3.194 ± 0.038	3.526LeuLys: 3.526 ± 0.037	9.041LeuLeu: 9.041 ± 0.077	1.385LeuMet: 1.385 ± 0.021	2.47LeuAsn: 2.47 ± 0.032	6.457LeuPro: 6.457 ± 0.047	3.463LeuGln: 3.463 ± 0.035	6.548LeuArg: 6.548 ± 0.051	7.625LeuSer: 7.625 ± 0.048	4.73LeuThr: 4.73 ± 0.035	6.495LeuVal: 6.495 ± 0.055	1.059LeuTrp: 1.059 ± 0.019	2.148LeuTyr: 2.148 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	1.814MetAla: 1.814 ± 0.026	0.174MetCys: 0.174 ± 0.007	0.897MetAsp: 0.897 ± 0.015	0.839MetGlu: 0.839 ± 0.015	0.526MetPhe: 0.526 ± 0.012	1.094MetGly: 1.094 ± 0.02	0.406MetHis: 0.406 ± 0.011	0.675MetIle: 0.675 ± 0.015	0.608MetLys: 0.608 ± 0.015	1.634MetLeu: 1.634 ± 0.022	0.393MetMet: 0.393 ± 0.012	0.473MetAsn: 0.473 ± 0.012	1.144MetPro: 1.144 ± 0.019	0.762MetGln: 0.762 ± 0.015	1.182MetArg: 1.182 ± 0.019	1.551MetSer: 1.551 ± 0.023	1.051MetThr: 1.051 ± 0.019	0.957MetVal: 0.957 ± 0.019	0.202MetTrp: 0.202 ± 0.008	0.42MetTyr: 0.42 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.917AsnAla: 2.917 ± 0.029	0.286AsnCys: 0.286 ± 0.01	1.486AsnAsp: 1.486 ± 0.025	1.558AsnGlu: 1.558 ± 0.017	0.886AsnPhe: 0.886 ± 0.018	2.457AsnGly: 2.457 ± 0.032	0.565AsnHis: 0.565 ± 0.013	1.04AsnIle: 1.04 ± 0.021	1.155AsnLys: 1.155 ± 0.019	2.638AsnLeu: 2.638 ± 0.029	0.456AsnMet: 0.456 ± 0.012	0.89AsnAsn: 0.89 ± 0.022	1.94AsnPro: 1.94 ± 0.027	0.854AsnGln: 0.854 ± 0.015	1.63AsnArg: 1.63 ± 0.022	2.006AsnSer: 2.006 ± 0.022	1.29AsnThr: 1.29 ± 0.02	1.797AsnVal: 1.797 ± 0.025	0.384AsnTrp: 0.384 ± 0.01	0.648AsnTyr: 0.648 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	7.961ProAla: 7.961 ± 0.076	0.439ProCys: 0.439 ± 0.012	3.259ProAsp: 3.259 ± 0.035	3.509ProGlu: 3.509 ± 0.031	2.145ProPhe: 2.145 ± 0.028	3.753ProGly: 3.753 ± 0.036	1.539ProHis: 1.539 ± 0.025	2.286ProIle: 2.286 ± 0.028	2.28ProLys: 2.28 ± 0.028	5.731ProLeu: 5.731 ± 0.046	0.878ProMet: 0.878 ± 0.017	1.832ProAsn: 1.832 ± 0.022	8.073ProPro: 8.073 ± 0.117	2.223ProGln: 2.223 ± 0.032	4.049ProArg: 4.049 ± 0.045	7.27ProSer: 7.27 ± 0.071	4.84ProThr: 4.84 ± 0.048	3.985ProVal: 3.985 ± 0.038	0.613ProTrp: 0.613 ± 0.015	1.423ProTyr: 1.423 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	3.997GlnAla: 3.997 ± 0.041	0.323GlnCys: 0.323 ± 0.01	1.896GlnAsp: 1.896 ± 0.024	2.138GlnGlu: 2.138 ± 0.024	1.04GlnPhe: 1.04 ± 0.018	2.187GlnGly: 2.187 ± 0.03	1.108GlnHis: 1.108 ± 0.019	1.499GlnIle: 1.499 ± 0.022	1.525GlnLys: 1.525 ± 0.021	3.678GlnLeu: 3.678 ± 0.032	0.684GlnMet: 0.684 ± 0.014	0.995GlnAsn: 0.995 ± 0.017	2.492GlnPro: 2.492 ± 0.036	3.195GlnGln: 3.195 ± 0.074	2.844GlnArg: 2.844 ± 0.027	2.622GlnSer: 2.622 ± 0.032	2.002GlnThr: 2.002 ± 0.026	2.285GlnVal: 2.285 ± 0.027	0.432GlnTrp: 0.432 ± 0.012	0.867GlnTyr: 0.867 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	6.648ArgAla: 6.648 ± 0.053	0.666ArgCys: 0.666 ± 0.014	3.659ArgAsp: 3.659 ± 0.036	4.239ArgGlu: 4.239 ± 0.046	2.245ArgPhe: 2.245 ± 0.028	4.157ArgGly: 4.157 ± 0.041	1.597ArgHis: 1.597 ± 0.025	2.795ArgIle: 2.795 ± 0.029	3.437ArgLys: 3.437 ± 0.035	6.44ArgLeu: 6.44 ± 0.05	1.233ArgMet: 1.233 ± 0.019	1.943ArgAsn: 1.943 ± 0.027	4.231ArgPro: 4.231 ± 0.044	2.819ArgGln: 2.819 ± 0.03	6.318ArgArg: 6.318 ± 0.063	5.529ArgSer: 5.529 ± 0.05	3.88ArgThr: 3.88 ± 0.038	3.863ArgVal: 3.863 ± 0.037	0.933ArgTrp: 0.933 ± 0.017	1.59ArgTyr: 1.59 ± 0.023	0.0ArgXaa: 0.0 ± 0.0
Ser	9.356SerAla: 9.356 ± 0.069	0.737SerCys: 0.737 ± 0.019	4.274SerAsp: 4.274 ± 0.042	3.844SerGlu: 3.844 ± 0.036	2.79SerPhe: 2.79 ± 0.03	5.833SerGly: 5.833 ± 0.049	1.971SerHis: 1.971 ± 0.028	2.986SerIle: 2.986 ± 0.032	2.87SerLys: 2.87 ± 0.03	7.876SerLeu: 7.876 ± 0.055	1.316SerMet: 1.316 ± 0.023	2.272SerAsn: 2.272 ± 0.028	6.662SerPro: 6.662 ± 0.078	2.636SerGln: 2.636 ± 0.032	5.536SerArg: 5.536 ± 0.052	11.714SerSer: 11.714 ± 0.138	6.367SerThr: 6.367 ± 0.053	4.497SerVal: 4.497 ± 0.037	0.92SerTrp: 0.92 ± 0.018	1.754SerTyr: 1.754 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	6.845ThrAla: 6.845 ± 0.057	0.638ThrCys: 0.638 ± 0.017	2.695ThrAsp: 2.695 ± 0.031	2.761ThrGlu: 2.761 ± 0.031	2.035ThrPhe: 2.035 ± 0.023	4.194ThrGly: 4.194 ± 0.042	1.147ThrHis: 1.147 ± 0.019	2.313ThrIle: 2.313 ± 0.033	2.06ThrLys: 2.06 ± 0.024	5.601ThrLeu: 5.601 ± 0.041	0.894ThrMet: 0.894 ± 0.017	1.502ThrAsn: 1.502 ± 0.022	4.53ThrPro: 4.53 ± 0.042	1.727ThrGln: 1.727 ± 0.023	3.545ThrArg: 3.545 ± 0.037	5.838ThrSer: 5.838 ± 0.046	4.876ThrThr: 4.876 ± 0.06	3.546ThrVal: 3.546 ± 0.032	0.707ThrTrp: 0.707 ± 0.017	1.344ThrTyr: 1.344 ± 0.021	0.0ThrXaa: 0.0 ± 0.0
Val	6.494ValAla: 6.494 ± 0.048	0.729ValCys: 0.729 ± 0.017	3.858ValAsp: 3.858 ± 0.038	4.24ValGlu: 4.24 ± 0.049	2.214ValPhe: 2.214 ± 0.029	4.171ValGly: 4.171 ± 0.039	1.398ValHis: 1.398 ± 0.022	2.419ValIle: 2.419 ± 0.028	2.744ValLys: 2.744 ± 0.034	5.921ValLeu: 5.921 ± 0.042	1.048ValMet: 1.048 ± 0.018	1.682ValAsn: 1.682 ± 0.023	4.083ValPro: 4.083 ± 0.041	2.385ValGln: 2.385 ± 0.025	4.073ValArg: 4.073 ± 0.037	4.513ValSer: 4.513 ± 0.037	3.263ValThr: 3.263 ± 0.029	4.7ValVal: 4.7 ± 0.038	0.854ValTrp: 0.854 ± 0.017	1.478ValTyr: 1.478 ± 0.023	0.0ValXaa: 0.0 ± 0.0
Trp	1.11TrpAla: 1.11 ± 0.019	0.169TrpCys: 0.169 ± 0.007	0.795TrpAsp: 0.795 ± 0.019	0.727TrpGlu: 0.727 ± 0.013	0.457TrpPhe: 0.457 ± 0.011	0.724TrpGly: 0.724 ± 0.015	0.308TrpHis: 0.308 ± 0.01	0.647TrpIle: 0.647 ± 0.016	0.584TrpLys: 0.584 ± 0.015	1.223TrpLeu: 1.223 ± 0.021	0.269TrpMet: 0.269 ± 0.009	0.443TrpAsn: 0.443 ± 0.012	0.526TrpPro: 0.526 ± 0.014	0.515TrpGln: 0.515 ± 0.012	0.998TrpArg: 0.998 ± 0.017	1.037TrpSer: 1.037 ± 0.022	0.906TrpThr: 0.906 ± 0.015	0.725TrpVal: 0.725 ± 0.014	0.242TrpTrp: 0.242 ± 0.009	0.338TrpTyr: 0.338 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.28TyrAla: 2.28 ± 0.03	0.308TyrCys: 0.308 ± 0.011	1.46TyrAsp: 1.46 ± 0.024	1.32TyrGlu: 1.32 ± 0.02	0.915TyrPhe: 0.915 ± 0.015	1.836TyrGly: 1.836 ± 0.028	0.592TyrHis: 0.592 ± 0.014	0.917TyrIle: 0.917 ± 0.018	0.858TyrLys: 0.858 ± 0.019	2.447TyrLeu: 2.447 ± 0.031	0.379TyrMet: 0.379 ± 0.01	0.731TyrAsn: 0.731 ± 0.014	1.286TyrPro: 1.286 ± 0.024	0.814TyrGln: 0.814 ± 0.017	1.553TyrArg: 1.553 ± 0.023	1.726TyrSer: 1.726 ± 0.023	1.275TyrThr: 1.275 ± 0.023	1.398TyrVal: 1.398 ± 0.023	0.335TyrTrp: 0.335 ± 0.01	0.695TyrTyr: 0.695 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6678 proteins (3457298 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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