Mucilaginibacter sp. ZH6
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4079 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3E2NT28|A0A3E2NT28_9SPHI Efflux transporter outer membrane subunit OS=Mucilaginibacter sp. ZH6 OX=2482727 GN=DYU05_00260 PE=3 SV=1
MM1 pKa = 7.64 SEE3 pKa = 4.16 ANYY6 pKa = 8.34 PTEE9 pKa = 4.87 GPDD12 pKa = 3.32 GTIPVQDD19 pKa = 3.53 GVNWTTNWRR28 pKa = 11.84 TYY30 pKa = 11.21 LDD32 pKa = 3.38 QSDD35 pKa = 3.89 NDD37 pKa = 3.25 FHH39 pKa = 7.96 IQYY42 pKa = 10.31 FNIPIVDD49 pKa = 4.44 FQNILTFNPGAEE61 pKa = 4.18 SVRR64 pKa = 11.84 AYY66 pKa = 9.88 IGLTDD71 pKa = 3.8 ATDD74 pKa = 3.32 PMTAKK79 pKa = 10.79 LILVPVVDD87 pKa = 3.99 GHH89 pKa = 6.38 DD90 pKa = 3.99 VIVVHH95 pKa = 7.04 PDD97 pKa = 2.86 LGGGVAGDD105 pKa = 4.08 GQSNIYY111 pKa = 11.09 DD112 pKa = 3.71 MTQACPPNCPANDD125 pKa = 4.0 SIMYY129 pKa = 9.86 AA130 pKa = 3.5
Molecular weight: 14.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.804
IPC2_protein 3.706
IPC_protein 3.694
Toseland 3.465
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.719
Rodwell 3.528
Grimsley 3.376
Solomon 3.706
Lehninger 3.656
Nozaki 3.846
DTASelect 4.139
Thurlkill 3.554
EMBOSS 3.719
Sillero 3.834
Patrickios 0.248
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|A0A3E2NQG5|A0A3E2NQG5_9SPHI Polymerase OS=Mucilaginibacter sp. ZH6 OX=2482727 GN=DYU05_13945 PE=4 SV=1
MM1 pKa = 7.34 KK2 pKa = 10.34 RR3 pKa = 11.84 ATFDD7 pKa = 3.28 STALTRR13 pKa = 11.84 LIKK16 pKa = 10.55 CCSFFMLMLSATIASAQKK34 pKa = 10.71 GKK36 pKa = 8.63 VTVVRR41 pKa = 11.84 DD42 pKa = 3.68 PLFDD46 pKa = 3.39 TLLAKK51 pKa = 10.35 RR52 pKa = 11.84 ALLNRR57 pKa = 11.84 KK58 pKa = 8.97 GGSSVARR65 pKa = 11.84 FTSSYY70 pKa = 10.7 GYY72 pKa = 10.67 RR73 pKa = 11.84 LQIYY77 pKa = 9.56 NGSNRR82 pKa = 11.84 SAAYY86 pKa = 9.51 AAQARR91 pKa = 11.84 FNRR94 pKa = 11.84 EE95 pKa = 3.55 FPEE98 pKa = 3.5 MRR100 pKa = 11.84 TYY102 pKa = 10.64 ISYY105 pKa = 10.54 RR106 pKa = 11.84 EE107 pKa = 3.95 PNFKK111 pKa = 10.14 VRR113 pKa = 11.84 AGDD116 pKa = 3.29 FRR118 pKa = 11.84 TRR120 pKa = 11.84 LEE122 pKa = 3.88 AEE124 pKa = 4.02 RR125 pKa = 11.84 LKK127 pKa = 11.06 SQLGNTFTAMFIVSEE142 pKa = 4.62 KK143 pKa = 10.34 INPPKK148 pKa = 10.24 TVINNDD154 pKa = 2.99
Molecular weight: 17.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.78
IPC_protein 10.467
Toseland 10.613
ProMoST 10.277
Dawson 10.73
Bjellqvist 10.423
Wikipedia 10.921
Rodwell 11.038
Grimsley 10.789
Solomon 10.818
Lehninger 10.774
Nozaki 10.599
DTASelect 10.423
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.657
Patrickios 10.745
IPC_peptide 10.818
IPC2_peptide 9.341
IPC2.peptide.svr19 8.557
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4079
0
4079
1373186
18
2339
336.6
37.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.922 ± 0.039
0.782 ± 0.011
5.449 ± 0.026
5.421 ± 0.042
4.808 ± 0.028
6.885 ± 0.036
1.892 ± 0.018
6.953 ± 0.035
6.834 ± 0.036
9.463 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.313 ± 0.019
5.525 ± 0.039
3.912 ± 0.023
3.749 ± 0.028
4.076 ± 0.021
6.146 ± 0.028
5.892 ± 0.037
6.675 ± 0.029
1.168 ± 0.014
4.134 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here