Amino acid dipepetide frequency for Mucilaginibacter sp. ZH6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.314AlaAla: 7.314 ± 0.089	0.693AlaCys: 0.693 ± 0.021	5.042AlaAsp: 5.042 ± 0.071	4.748AlaGlu: 4.748 ± 0.075	3.646AlaPhe: 3.646 ± 0.053	6.169AlaGly: 6.169 ± 0.08	1.328AlaHis: 1.328 ± 0.03	5.571AlaIle: 5.571 ± 0.076	4.961AlaLys: 4.961 ± 0.067	7.289AlaLeu: 7.289 ± 0.077	1.901AlaMet: 1.901 ± 0.039	3.911AlaAsn: 3.911 ± 0.06	2.598AlaPro: 2.598 ± 0.056	3.016AlaGln: 3.016 ± 0.045	2.84AlaArg: 2.84 ± 0.042	4.558AlaSer: 4.558 ± 0.062	4.425AlaThr: 4.425 ± 0.058	5.481AlaVal: 5.481 ± 0.066	0.787AlaTrp: 0.787 ± 0.026	2.942AlaTyr: 2.942 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.561CysAla: 0.561 ± 0.02	0.125CysCys: 0.125 ± 0.01	0.398CysAsp: 0.398 ± 0.018	0.369CysGlu: 0.369 ± 0.018	0.433CysPhe: 0.433 ± 0.018	0.627CysGly: 0.627 ± 0.025	0.205CysHis: 0.205 ± 0.014	0.612CysIle: 0.612 ± 0.022	0.451CysLys: 0.451 ± 0.018	0.79CysLeu: 0.79 ± 0.025	0.181CysMet: 0.181 ± 0.011	0.377CysAsn: 0.377 ± 0.017	0.303CysPro: 0.303 ± 0.017	0.221CysGln: 0.221 ± 0.012	0.329CysArg: 0.329 ± 0.016	0.527CysSer: 0.527 ± 0.02	0.433CysThr: 0.433 ± 0.018	0.467CysVal: 0.467 ± 0.021	0.095CysTrp: 0.095 ± 0.009	0.312CysTyr: 0.312 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	4.364AspAla: 4.364 ± 0.06	0.385AspCys: 0.385 ± 0.02	2.83AspAsp: 2.83 ± 0.058	3.49AspGlu: 3.49 ± 0.062	2.829AspPhe: 2.829 ± 0.052	3.801AspGly: 3.801 ± 0.064	1.041AspHis: 1.041 ± 0.029	3.865AspIle: 3.865 ± 0.053	4.015AspLys: 4.015 ± 0.055	4.848AspLeu: 4.848 ± 0.068	1.236AspMet: 1.236 ± 0.031	2.91AspAsn: 2.91 ± 0.048	2.108AspPro: 2.108 ± 0.041	1.783AspGln: 1.783 ± 0.04	2.146AspArg: 2.146 ± 0.044	2.882AspSer: 2.882 ± 0.048	2.861AspThr: 2.861 ± 0.046	3.865AspVal: 3.865 ± 0.046	0.786AspTrp: 0.786 ± 0.024	2.448AspTyr: 2.448 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	4.209GluAla: 4.209 ± 0.066	0.315GluCys: 0.315 ± 0.016	2.797GluAsp: 2.797 ± 0.053	3.496GluGlu: 3.496 ± 0.068	2.332GluPhe: 2.332 ± 0.043	3.246GluGly: 3.246 ± 0.05	1.151GluHis: 1.151 ± 0.033	3.793GluIle: 3.793 ± 0.069	4.115GluLys: 4.115 ± 0.067	5.755GluLeu: 5.755 ± 0.076	1.449GluMet: 1.449 ± 0.035	2.872GluAsn: 2.872 ± 0.053	1.74GluPro: 1.74 ± 0.037	2.539GluGln: 2.539 ± 0.049	2.627GluArg: 2.627 ± 0.053	2.707GluSer: 2.707 ± 0.045	2.695GluThr: 2.695 ± 0.047	3.721GluVal: 3.721 ± 0.056	0.634GluTrp: 0.634 ± 0.023	2.02GluTyr: 2.02 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	3.594PheAla: 3.594 ± 0.05	0.446PheCys: 0.446 ± 0.019	2.877PheAsp: 2.877 ± 0.048	2.568PheGlu: 2.568 ± 0.045	2.371PhePhe: 2.371 ± 0.055	3.466PheGly: 3.466 ± 0.059	0.827PheHis: 0.827 ± 0.029	3.384PheIle: 3.384 ± 0.056	3.332PheLys: 3.332 ± 0.057	4.129PheLeu: 4.129 ± 0.061	1.119PheMet: 1.119 ± 0.033	2.945PheAsn: 2.945 ± 0.051	1.676PhePro: 1.676 ± 0.036	1.233PheGln: 1.233 ± 0.028	1.779PheArg: 1.779 ± 0.039	3.387PheSer: 3.387 ± 0.058	3.262PheThr: 3.262 ± 0.052	2.951PheVal: 2.951 ± 0.048	0.607PheTrp: 0.607 ± 0.023	2.129PheTyr: 2.129 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	4.902GlyAla: 4.902 ± 0.073	0.617GlyCys: 0.617 ± 0.025	3.513GlyAsp: 3.513 ± 0.052	3.375GlyGlu: 3.375 ± 0.049	3.655GlyPhe: 3.655 ± 0.053	5.119GlyGly: 5.119 ± 0.09	1.314GlyHis: 1.314 ± 0.033	5.244GlyIle: 5.244 ± 0.068	5.226GlyLys: 5.226 ± 0.066	6.27GlyLeu: 6.27 ± 0.065	1.668GlyMet: 1.668 ± 0.034	3.641GlyAsn: 3.641 ± 0.075	1.733GlyPro: 1.733 ± 0.046	2.3GlyGln: 2.3 ± 0.047	2.769GlyArg: 2.769 ± 0.049	4.378GlySer: 4.378 ± 0.067	4.288GlyThr: 4.288 ± 0.082	4.714GlyVal: 4.714 ± 0.06	0.971GlyTrp: 0.971 ± 0.022	3.155GlyTyr: 3.155 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	1.185HisAla: 1.185 ± 0.027	0.173HisCys: 0.173 ± 0.012	1.024HisAsp: 1.024 ± 0.026	0.979HisGlu: 0.979 ± 0.03	1.082HisPhe: 1.082 ± 0.03	1.219HisGly: 1.219 ± 0.033	0.522HisHis: 0.522 ± 0.022	1.396HisIle: 1.396 ± 0.032	1.056HisLys: 1.056 ± 0.029	1.956HisLeu: 1.956 ± 0.042	0.397HisMet: 0.397 ± 0.019	0.958HisAsn: 0.958 ± 0.025	1.041HisPro: 1.041 ± 0.029	0.795HisGln: 0.795 ± 0.026	0.802HisArg: 0.802 ± 0.023	1.068HisSer: 1.068 ± 0.028	1.061HisThr: 1.061 ± 0.03	1.063HisVal: 1.063 ± 0.027	0.259HisTrp: 0.259 ± 0.013	0.887HisTyr: 0.887 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.042IleAla: 6.042 ± 0.076	0.65IleCys: 0.65 ± 0.025	4.139IleAsp: 4.139 ± 0.055	3.717IleGlu: 3.717 ± 0.063	2.956IlePhe: 2.956 ± 0.051	4.711IleGly: 4.711 ± 0.058	1.199IleHis: 1.199 ± 0.034	4.893IleIle: 4.893 ± 0.081	4.827IleLys: 4.827 ± 0.061	5.849IleLeu: 5.849 ± 0.089	1.395IleMet: 1.395 ± 0.036	4.149IleAsn: 4.149 ± 0.06	2.838IlePro: 2.838 ± 0.044	2.063IleGln: 2.063 ± 0.038	2.918IleArg: 2.918 ± 0.053	4.89IleSer: 4.89 ± 0.054	4.633IleThr: 4.633 ± 0.062	4.324IleVal: 4.324 ± 0.068	0.685IleTrp: 0.685 ± 0.026	2.651IleTyr: 2.651 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	5.63LysAla: 5.63 ± 0.076	0.326LysCys: 0.326 ± 0.016	3.814LysAsp: 3.814 ± 0.054	4.102LysGlu: 4.102 ± 0.076	2.666LysPhe: 2.666 ± 0.049	4.375LysGly: 4.375 ± 0.061	1.328LysHis: 1.328 ± 0.032	4.453LysIle: 4.453 ± 0.056	4.951LysLys: 4.951 ± 0.078	6.404LysLeu: 6.404 ± 0.074	1.803LysMet: 1.803 ± 0.041	3.739LysAsn: 3.739 ± 0.06	2.968LysPro: 2.968 ± 0.055	3.035LysGln: 3.035 ± 0.051	2.95LysArg: 2.95 ± 0.049	3.621LysSer: 3.621 ± 0.052	3.937LysThr: 3.937 ± 0.058	4.595LysVal: 4.595 ± 0.058	0.797LysTrp: 0.797 ± 0.026	2.844LysTyr: 2.844 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	7.303LeuAla: 7.303 ± 0.082	0.804LeuCys: 0.804 ± 0.026	4.682LeuAsp: 4.682 ± 0.058	4.508LeuGlu: 4.508 ± 0.074	4.557LeuPhe: 4.557 ± 0.066	5.766LeuGly: 5.766 ± 0.079	1.755LeuHis: 1.755 ± 0.034	6.268LeuIle: 6.268 ± 0.077	6.982LeuLys: 6.982 ± 0.076	9.78LeuLeu: 9.78 ± 0.118	2.23LeuMet: 2.23 ± 0.048	5.481LeuAsn: 5.481 ± 0.07	4.191LeuPro: 4.191 ± 0.06	3.834LeuGln: 3.834 ± 0.052	3.859LeuArg: 3.859 ± 0.058	6.7LeuSer: 6.7 ± 0.086	5.754LeuThr: 5.754 ± 0.067	5.92LeuVal: 5.92 ± 0.069	0.919LeuTrp: 0.919 ± 0.028	3.34LeuTyr: 3.34 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	1.926MetAla: 1.926 ± 0.04	0.159MetCys: 0.159 ± 0.011	1.165MetAsp: 1.165 ± 0.027	1.29MetGlu: 1.29 ± 0.035	0.896MetPhe: 0.896 ± 0.025	1.564MetGly: 1.564 ± 0.039	0.479MetHis: 0.479 ± 0.02	1.5MetIle: 1.5 ± 0.032	1.851MetLys: 1.851 ± 0.038	2.323MetLeu: 2.323 ± 0.042	0.631MetMet: 0.631 ± 0.024	1.237MetAsn: 1.237 ± 0.032	1.158MetPro: 1.158 ± 0.028	1.033MetGln: 1.033 ± 0.031	1.043MetArg: 1.043 ± 0.029	1.411MetSer: 1.411 ± 0.034	1.052MetThr: 1.052 ± 0.031	1.531MetVal: 1.531 ± 0.028	0.189MetTrp: 0.189 ± 0.011	0.694MetTyr: 0.694 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	4.064AsnAla: 4.064 ± 0.06	0.403AsnCys: 0.403 ± 0.017	2.833AsnAsp: 2.833 ± 0.057	2.756AsnGlu: 2.756 ± 0.044	2.669AsnPhe: 2.669 ± 0.045	4.107AsnGly: 4.107 ± 0.083	0.997AsnHis: 0.997 ± 0.024	4.027AsnIle: 4.027 ± 0.06	3.584AsnLys: 3.584 ± 0.053	4.914AsnLeu: 4.914 ± 0.059	1.178AsnMet: 1.178 ± 0.028	3.444AsnAsn: 3.444 ± 0.061	2.593AsnPro: 2.593 ± 0.046	1.973AsnGln: 1.973 ± 0.038	2.343AsnArg: 2.343 ± 0.049	3.27AsnSer: 3.27 ± 0.059	3.362AsnThr: 3.362 ± 0.057	3.363AsnVal: 3.363 ± 0.058	0.722AsnTrp: 0.722 ± 0.023	2.651AsnTyr: 2.651 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	3.619ProAla: 3.619 ± 0.058	0.222ProCys: 0.222 ± 0.012	2.619ProAsp: 2.619 ± 0.05	2.858ProGlu: 2.858 ± 0.051	1.892ProPhe: 1.892 ± 0.038	3.031ProGly: 3.031 ± 0.052	0.722ProHis: 0.722 ± 0.024	2.251ProIle: 2.251 ± 0.042	2.214ProLys: 2.214 ± 0.044	3.408ProLeu: 3.408 ± 0.049	0.781ProMet: 0.781 ± 0.025	2.005ProAsn: 2.005 ± 0.041	1.194ProPro: 1.194 ± 0.036	1.485ProGln: 1.485 ± 0.034	1.183ProArg: 1.183 ± 0.027	2.212ProSer: 2.212 ± 0.043	2.022ProThr: 2.022 ± 0.045	3.371ProVal: 3.371 ± 0.054	0.425ProTrp: 0.425 ± 0.017	1.609ProTyr: 1.609 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	2.803GlnAla: 2.803 ± 0.049	0.193GlnCys: 0.193 ± 0.013	1.711GlnAsp: 1.711 ± 0.036	1.976GlnGlu: 1.976 ± 0.042	1.644GlnPhe: 1.644 ± 0.034	2.079GlnGly: 2.079 ± 0.045	0.848GlnHis: 0.848 ± 0.026	2.379GlnIle: 2.379 ± 0.05	2.57GlnLys: 2.57 ± 0.042	3.968GlnLeu: 3.968 ± 0.062	0.954GlnMet: 0.954 ± 0.028	1.957GlnAsn: 1.957 ± 0.042	1.614GlnPro: 1.614 ± 0.037	2.26GlnGln: 2.26 ± 0.097	1.699GlnArg: 1.699 ± 0.045	2.214GlnSer: 2.214 ± 0.038	2.142GlnThr: 2.142 ± 0.046	2.574GlnVal: 2.574 ± 0.044	0.421GlnTrp: 0.421 ± 0.017	1.48GlnTyr: 1.48 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	2.634ArgAla: 2.634 ± 0.046	0.245ArgCys: 0.245 ± 0.014	2.184ArgAsp: 2.184 ± 0.046	2.364ArgGlu: 2.364 ± 0.043	2.148ArgPhe: 2.148 ± 0.042	2.268ArgGly: 2.268 ± 0.049	0.766ArgHis: 0.766 ± 0.026	3.035ArgIle: 3.035 ± 0.052	2.956ArgLys: 2.956 ± 0.052	4.027ArgLeu: 4.027 ± 0.06	1.103ArgMet: 1.103 ± 0.028	2.332ArgAsn: 2.332 ± 0.047	1.51ArgPro: 1.51 ± 0.037	1.615ArgGln: 1.615 ± 0.045	1.807ArgArg: 1.807 ± 0.041	2.424ArgSer: 2.424 ± 0.044	2.22ArgThr: 2.22 ± 0.04	2.643ArgVal: 2.643 ± 0.044	0.53ArgTrp: 0.53 ± 0.022	1.951ArgTyr: 1.951 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	5.037SerAla: 5.037 ± 0.07	0.54SerCys: 0.54 ± 0.019	2.888SerAsp: 2.888 ± 0.044	2.802SerGlu: 2.802 ± 0.045	3.355SerPhe: 3.355 ± 0.055	4.835SerGly: 4.835 ± 0.085	1.101SerHis: 1.101 ± 0.029	4.415SerIle: 4.415 ± 0.055	3.832SerLys: 3.832 ± 0.056	5.899SerLeu: 5.899 ± 0.074	1.254SerMet: 1.254 ± 0.034	3.151SerAsn: 3.151 ± 0.058	2.459SerPro: 2.459 ± 0.047	2.008SerGln: 2.008 ± 0.039	2.547SerArg: 2.547 ± 0.044	3.951SerSer: 3.951 ± 0.071	3.691SerThr: 3.691 ± 0.058	4.246SerVal: 4.246 ± 0.068	0.748SerTrp: 0.748 ± 0.026	2.699SerTyr: 2.699 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	5.058ThrAla: 5.058 ± 0.064	0.398ThrCys: 0.398 ± 0.02	3.498ThrAsp: 3.498 ± 0.046	2.994ThrGlu: 2.994 ± 0.058	2.856ThrPhe: 2.856 ± 0.048	4.98ThrGly: 4.98 ± 0.075	1.02ThrHis: 1.02 ± 0.028	4.284ThrIle: 4.284 ± 0.064	3.212ThrLys: 3.212 ± 0.051	5.444ThrLeu: 5.444 ± 0.069	1.049ThrMet: 1.049 ± 0.029	2.949ThrAsn: 2.949 ± 0.06	2.713ThrPro: 2.713 ± 0.048	1.867ThrGln: 1.867 ± 0.04	2.14ThrArg: 2.14 ± 0.043	3.581ThrSer: 3.581 ± 0.061	3.611ThrThr: 3.611 ± 0.064	4.138ThrVal: 4.138 ± 0.061	0.666ThrTrp: 0.666 ± 0.025	2.464ThrTyr: 2.464 ± 0.054	0.0ThrXaa: 0.0 ± 0.0
Val	5.301ValAla: 5.301 ± 0.07	0.636ValCys: 0.636 ± 0.021	3.587ValAsp: 3.587 ± 0.057	3.331ValGlu: 3.331 ± 0.056	3.23ValPhe: 3.23 ± 0.054	3.879ValGly: 3.879 ± 0.059	1.133ValHis: 1.133 ± 0.028	4.875ValIle: 4.875 ± 0.059	4.726ValLys: 4.726 ± 0.062	6.358ValLeu: 6.358 ± 0.076	1.662ValMet: 1.662 ± 0.038	3.94ValAsn: 3.94 ± 0.057	2.608ValPro: 2.608 ± 0.045	2.142ValGln: 2.142 ± 0.039	2.505ValArg: 2.505 ± 0.045	4.385ValSer: 4.385 ± 0.054	4.252ValThr: 4.252 ± 0.064	4.821ValVal: 4.821 ± 0.075	0.711ValTrp: 0.711 ± 0.023	2.67ValTyr: 2.67 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.807TrpAla: 0.807 ± 0.024	0.122TrpCys: 0.122 ± 0.01	0.669TrpAsp: 0.669 ± 0.024	0.572TrpGlu: 0.572 ± 0.023	0.626TrpPhe: 0.626 ± 0.025	0.851TrpGly: 0.851 ± 0.025	0.292TrpHis: 0.292 ± 0.015	0.712TrpIle: 0.712 ± 0.027	0.754TrpLys: 0.754 ± 0.025	1.221TrpLeu: 1.221 ± 0.034	0.365TrpMet: 0.365 ± 0.016	0.618TrpAsn: 0.618 ± 0.022	0.382TrpPro: 0.382 ± 0.018	0.553TrpGln: 0.553 ± 0.021	0.513TrpArg: 0.513 ± 0.019	0.639TrpSer: 0.639 ± 0.024	0.62TrpThr: 0.62 ± 0.021	0.69TrpVal: 0.69 ± 0.024	0.218TrpTrp: 0.218 ± 0.014	0.452TrpTyr: 0.452 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.867TyrAla: 2.867 ± 0.051	0.363TyrCys: 0.363 ± 0.016	2.227TyrAsp: 2.227 ± 0.036	1.92TyrGlu: 1.92 ± 0.04	2.237TyrPhe: 2.237 ± 0.04	2.761TyrGly: 2.761 ± 0.046	0.895TyrHis: 0.895 ± 0.03	2.572TyrIle: 2.572 ± 0.041	2.724TyrLys: 2.724 ± 0.048	4.0TyrLeu: 4.0 ± 0.062	0.778TyrMet: 0.778 ± 0.026	2.635TyrAsn: 2.635 ± 0.058	1.692TyrPro: 1.692 ± 0.035	1.732TyrGln: 1.732 ± 0.033	1.959TyrArg: 1.959 ± 0.035	2.651TyrSer: 2.651 ± 0.048	2.601TyrThr: 2.601 ± 0.049	2.276TyrVal: 2.276 ± 0.038	0.51TyrTrp: 0.51 ± 0.021	1.936TyrTyr: 1.936 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4079 proteins (1373186 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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