Halomonas sp. 204

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3670 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4HEG4|A0A4R4HEG4_9GAMM PaaI family thioesterase OS=Halomonas sp. 204 OX=2546095 GN=E0702_09650 PE=4 SV=1
MM1 pKa = 7.51SEE3 pKa = 4.38AEE5 pKa = 4.47SFVPTPLMLSDD16 pKa = 3.75SARR19 pKa = 11.84ARR21 pKa = 11.84LRR23 pKa = 11.84ALIAEE28 pKa = 4.38QGNSEE33 pKa = 4.32LKK35 pKa = 10.58LRR37 pKa = 11.84VYY39 pKa = 9.1VTGGGCSGFQYY50 pKa = 10.98GFDD53 pKa = 3.83FADD56 pKa = 5.91DD57 pKa = 3.76IADD60 pKa = 4.59DD61 pKa = 3.94DD62 pKa = 4.37TVIAFGDD69 pKa = 3.6VALVVDD75 pKa = 4.48PLSYY79 pKa = 10.58QYY81 pKa = 11.59LVGSTVDD88 pKa = 3.64YY89 pKa = 11.51EE90 pKa = 4.24EE91 pKa = 4.76GLAGARR97 pKa = 11.84FLVQNPNASTTCGCGASFMVV117 pKa = 4.17

Molecular weight: 12.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R4HE51|A0A4R4HE51_9GAMM Glutamate-1-semialdehyde 2 1-aminomutase OS=Halomonas sp. 204 OX=2546095 GN=hemL PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.17RR12 pKa = 11.84KK13 pKa = 9.53RR14 pKa = 11.84MHH16 pKa = 6.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3670

0

3670

1072441

22

2778

292.2

32.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.24 ± 0.056

0.919 ± 0.014

5.596 ± 0.038

6.833 ± 0.051

3.393 ± 0.029

8.391 ± 0.04

2.435 ± 0.024

4.595 ± 0.037

2.641 ± 0.037

11.693 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.02

2.473 ± 0.028

4.999 ± 0.028

3.591 ± 0.029

7.491 ± 0.055

5.321 ± 0.027

4.964 ± 0.037

7.229 ± 0.034

1.451 ± 0.02

2.275 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski