Streptococcus satellite phage Javan302
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJW9|A0A4D5ZJW9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan302 OX=2558622 GN=JavanS302_0006 PE=4 SV=1
MM1 pKa = 7.34 TLDD4 pKa = 4.82 LDD6 pKa = 4.12 NMTQAEE12 pKa = 4.39 FDD14 pKa = 3.59 EE15 pKa = 4.86 VMAEE19 pKa = 4.01 IKK21 pKa = 10.22 EE22 pKa = 4.0 KK23 pKa = 10.73 RR24 pKa = 11.84 PNLFQFIADD33 pKa = 3.79 FVDD36 pKa = 3.81 RR37 pKa = 11.84 KK38 pKa = 10.81 VSTEE42 pKa = 3.88 EE43 pKa = 3.34 VDD45 pKa = 4.23 EE46 pKa = 4.09 YY47 pKa = 11.74 LKK49 pKa = 10.4 MKK51 pKa = 10.42 HH52 pKa = 5.45 EE53 pKa = 4.23 KK54 pKa = 9.64 QVEE57 pKa = 4.72 FIKK60 pKa = 10.72 NYY62 pKa = 6.92 QARR65 pKa = 11.84 AA66 pKa = 3.37
Molecular weight: 7.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.861
IPC2_protein 4.825
IPC_protein 4.66
Toseland 4.52
ProMoST 4.762
Dawson 4.596
Bjellqvist 4.749
Wikipedia 4.457
Rodwell 4.507
Grimsley 4.431
Solomon 4.596
Lehninger 4.546
Nozaki 4.711
DTASelect 4.838
Thurlkill 4.52
EMBOSS 4.482
Sillero 4.774
Patrickios 4.304
IPC_peptide 4.609
IPC2_peptide 4.762
IPC2.peptide.svr19 4.768
Protein with the highest isoelectric point:
>tr|A0A4D5ZNQ8|A0A4D5ZNQ8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan302 OX=2558622 GN=JavanS302_0012 PE=4 SV=1
MM1 pKa = 7.56 FDD3 pKa = 4.24 SFPEE7 pKa = 3.89 RR8 pKa = 11.84 PKK10 pKa = 10.37 YY11 pKa = 9.41 MKK13 pKa = 9.51 RR14 pKa = 11.84 DD15 pKa = 3.77 RR16 pKa = 11.84 YY17 pKa = 9.18 HH18 pKa = 5.94 KK19 pKa = 10.09 HH20 pKa = 4.58 YY21 pKa = 11.02 KK22 pKa = 10.26 KK23 pKa = 10.36 FLKK26 pKa = 8.87 YY27 pKa = 8.46 TKK29 pKa = 10.33 KK30 pKa = 10.73 GDD32 pKa = 3.84 RR33 pKa = 11.84 FWLNGLGSLRR43 pKa = 3.91
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.246
IPC2_protein 9.853
IPC_protein 10.116
Toseland 10.511
ProMoST 10.277
Dawson 10.643
Bjellqvist 10.292
Wikipedia 10.804
Rodwell 11.184
Grimsley 10.701
Solomon 10.687
Lehninger 10.657
Nozaki 10.467
DTASelect 10.292
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.555
Patrickios 10.95
IPC_peptide 10.687
IPC2_peptide 8.887
IPC2.peptide.svr19 8.697
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
2613
43
492
153.7
17.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.626 ± 0.279
0.574 ± 0.126
5.358 ± 0.47
8.726 ± 0.764
4.325 ± 0.412
5.281 ± 0.34
2.028 ± 0.319
6.582 ± 0.592
9.568 ± 0.578
9.529 ± 0.527
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.717 ± 0.356
5.281 ± 0.632
3.559 ± 0.765
3.789 ± 0.436
5.281 ± 0.486
5.166 ± 0.514
6.315 ± 0.246
5.281 ± 0.391
0.804 ± 0.237
4.21 ± 0.508
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here