Amino acid dipepetide frequency for Gordonia phage PhrostedPhlake

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.437AlaAla: 17.437 ± 1.349
0.766AlaCys: 0.766 ± 0.248
7.658AlaAsp: 7.658 ± 0.666
6.716AlaGlu: 6.716 ± 0.676
3.711AlaPhe: 3.711 ± 0.493
9.602AlaGly: 9.602 ± 0.965
1.708AlaHis: 1.708 ± 0.32
5.361AlaIle: 5.361 ± 0.5
3.181AlaLys: 3.181 ± 0.634
9.661AlaLeu: 9.661 ± 0.893
2.769AlaMet: 2.769 ± 0.422
3.24AlaAsn: 3.24 ± 0.464
5.714AlaPro: 5.714 ± 0.572
5.302AlaGln: 5.302 ± 0.632
7.482AlaArg: 7.482 ± 0.735
5.832AlaSer: 5.832 ± 0.682
7.069AlaThr: 7.069 ± 0.718
8.66AlaVal: 8.66 ± 0.937
2.121AlaTrp: 2.121 ± 0.351
2.297AlaTyr: 2.297 ± 0.333
0.0AlaXaa: 0.0 ± 0.0
Cys
0.943CysAla: 0.943 ± 0.25
0.177CysCys: 0.177 ± 0.126
1.355CysAsp: 1.355 ± 0.357
0.589CysGlu: 0.589 ± 0.207
0.0CysPhe: 0.0 ± 0.0
1.178CysGly: 1.178 ± 0.301
0.589CysHis: 0.589 ± 0.234
0.295CysIle: 0.295 ± 0.133
0.118CysLys: 0.118 ± 0.087
0.471CysLeu: 0.471 ± 0.167
0.471CysMet: 0.471 ± 0.173
0.353CysAsn: 0.353 ± 0.164
1.119CysPro: 1.119 ± 0.277
0.412CysGln: 0.412 ± 0.152
0.471CysArg: 0.471 ± 0.157
0.471CysSer: 0.471 ± 0.162
0.589CysThr: 0.589 ± 0.171
0.353CysVal: 0.353 ± 0.144
0.295CysTrp: 0.295 ± 0.156
0.059CysTyr: 0.059 ± 0.063
0.0CysXaa: 0.0 ± 0.0
Asp
7.128AspAla: 7.128 ± 0.749
0.648AspCys: 0.648 ± 0.209
5.184AspAsp: 5.184 ± 0.707
5.066AspGlu: 5.066 ± 0.649
1.649AspPhe: 1.649 ± 0.309
6.775AspGly: 6.775 ± 0.711
1.767AspHis: 1.767 ± 0.383
2.769AspIle: 2.769 ± 0.399
1.355AspLys: 1.355 ± 0.3
6.009AspLeu: 6.009 ± 0.765
1.473AspMet: 1.473 ± 0.263
2.062AspAsn: 2.062 ± 0.359
3.947AspPro: 3.947 ± 0.548
2.003AspGln: 2.003 ± 0.304
5.302AspArg: 5.302 ± 0.659
2.887AspSer: 2.887 ± 0.418
4.006AspThr: 4.006 ± 0.539
5.655AspVal: 5.655 ± 0.517
1.237AspTrp: 1.237 ± 0.249
2.239AspTyr: 2.239 ± 0.439
0.0AspXaa: 0.0 ± 0.0
Glu
4.595GluAla: 4.595 ± 0.54
0.589GluCys: 0.589 ± 0.196
3.417GluAsp: 3.417 ± 0.48
1.944GluGlu: 1.944 ± 0.449
1.944GluPhe: 1.944 ± 0.339
4.124GluGly: 4.124 ± 0.415
1.296GluHis: 1.296 ± 0.272
2.474GluIle: 2.474 ± 0.355
2.297GluLys: 2.297 ± 0.406
5.538GluLeu: 5.538 ± 0.69
1.591GluMet: 1.591 ± 0.314
1.591GluAsn: 1.591 ± 0.281
3.652GluPro: 3.652 ± 0.577
2.71GluGln: 2.71 ± 0.486
4.477GluArg: 4.477 ± 0.45
2.769GluSer: 2.769 ± 0.387
3.063GluThr: 3.063 ± 0.371
5.243GluVal: 5.243 ± 0.655
1.355GluTrp: 1.355 ± 0.292
1.119GluTyr: 1.119 ± 0.287
0.0GluXaa: 0.0 ± 0.0
Phe
2.887PheAla: 2.887 ± 0.375
0.412PheCys: 0.412 ± 0.13
2.239PheAsp: 2.239 ± 0.244
1.767PheGlu: 1.767 ± 0.306
0.884PhePhe: 0.884 ± 0.22
2.415PheGly: 2.415 ± 0.416
0.471PheHis: 0.471 ± 0.193
0.825PheIle: 0.825 ± 0.269
0.943PheLys: 0.943 ± 0.236
1.826PheLeu: 1.826 ± 0.381
0.471PheMet: 0.471 ± 0.153
0.648PheAsn: 0.648 ± 0.244
1.885PhePro: 1.885 ± 0.277
0.648PheGln: 0.648 ± 0.17
1.767PheArg: 1.767 ± 0.297
1.591PheSer: 1.591 ± 0.374
2.297PheThr: 2.297 ± 0.404
2.828PheVal: 2.828 ± 0.396
0.236PheTrp: 0.236 ± 0.108
0.589PheTyr: 0.589 ± 0.184
0.0PheXaa: 0.0 ± 0.0
Gly
9.013GlyAla: 9.013 ± 1.12
0.589GlyCys: 0.589 ± 0.193
5.42GlyAsp: 5.42 ± 0.5
4.772GlyGlu: 4.772 ± 0.62
3.476GlyPhe: 3.476 ± 0.48
7.835GlyGly: 7.835 ± 0.649
2.18GlyHis: 2.18 ± 0.535
4.124GlyIle: 4.124 ± 0.686
3.535GlyLys: 3.535 ± 0.511
7.423GlyLeu: 7.423 ± 1.053
1.532GlyMet: 1.532 ± 0.297
2.71GlyAsn: 2.71 ± 0.355
4.418GlyPro: 4.418 ± 0.54
2.533GlyGln: 2.533 ± 0.33
6.244GlyArg: 6.244 ± 0.58
4.654GlySer: 4.654 ± 0.55
4.948GlyThr: 4.948 ± 0.571
6.539GlyVal: 6.539 ± 0.515
2.18GlyTrp: 2.18 ± 0.382
2.71GlyTyr: 2.71 ± 0.383
0.0GlyXaa: 0.0 ± 0.0
His
2.769HisAla: 2.769 ± 0.342
0.295HisCys: 0.295 ± 0.135
1.708HisAsp: 1.708 ± 0.28
0.648HisGlu: 0.648 ± 0.19
0.53HisPhe: 0.53 ± 0.201
1.414HisGly: 1.414 ± 0.294
0.589HisHis: 0.589 ± 0.203
1.06HisIle: 1.06 ± 0.219
0.589HisLys: 0.589 ± 0.152
2.18HisLeu: 2.18 ± 0.394
0.118HisMet: 0.118 ± 0.074
0.295HisAsn: 0.295 ± 0.143
1.767HisPro: 1.767 ± 0.341
0.943HisGln: 0.943 ± 0.233
2.003HisArg: 2.003 ± 0.395
1.06HisSer: 1.06 ± 0.244
1.355HisThr: 1.355 ± 0.308
1.591HisVal: 1.591 ± 0.272
0.53HisTrp: 0.53 ± 0.188
0.589HisTyr: 0.589 ± 0.183
0.0HisXaa: 0.0 ± 0.0
Ile
6.362IleAla: 6.362 ± 0.638
0.236IleCys: 0.236 ± 0.112
4.124IleAsp: 4.124 ± 0.609
2.18IleGlu: 2.18 ± 0.299
0.825IlePhe: 0.825 ± 0.203
5.243IleGly: 5.243 ± 0.695
0.53IleHis: 0.53 ± 0.153
1.414IleIle: 1.414 ± 0.279
1.414IleLys: 1.414 ± 0.356
2.769IleLeu: 2.769 ± 0.431
0.177IleMet: 0.177 ± 0.092
1.001IleAsn: 1.001 ± 0.216
3.24IlePro: 3.24 ± 0.371
0.884IleGln: 0.884 ± 0.197
3.711IleArg: 3.711 ± 0.415
1.885IleSer: 1.885 ± 0.344
3.358IleThr: 3.358 ± 0.335
3.829IleVal: 3.829 ± 0.458
0.177IleTrp: 0.177 ± 0.099
0.825IleTyr: 0.825 ± 0.215
0.0IleXaa: 0.0 ± 0.0
Lys
3.77LysAla: 3.77 ± 0.574
0.236LysCys: 0.236 ± 0.122
1.473LysAsp: 1.473 ± 0.334
1.473LysGlu: 1.473 ± 0.35
1.178LysPhe: 1.178 ± 0.314
2.356LysGly: 2.356 ± 0.402
0.707LysHis: 0.707 ± 0.258
1.532LysIle: 1.532 ± 0.304
1.649LysLys: 1.649 ± 0.301
2.651LysLeu: 2.651 ± 0.392
0.53LysMet: 0.53 ± 0.167
1.119LysAsn: 1.119 ± 0.295
2.356LysPro: 2.356 ± 0.352
1.001LysGln: 1.001 ± 0.268
2.18LysArg: 2.18 ± 0.364
1.826LysSer: 1.826 ± 0.293
2.18LysThr: 2.18 ± 0.336
2.356LysVal: 2.356 ± 0.37
0.53LysTrp: 0.53 ± 0.159
0.766LysTyr: 0.766 ± 0.23
0.0LysXaa: 0.0 ± 0.0
Leu
10.486LeuAla: 10.486 ± 0.866
0.943LeuCys: 0.943 ± 0.27
5.361LeuAsp: 5.361 ± 0.714
3.652LeuGlu: 3.652 ± 0.555
1.944LeuPhe: 1.944 ± 0.267
6.539LeuGly: 6.539 ± 0.653
1.178LeuHis: 1.178 ± 0.283
3.358LeuIle: 3.358 ± 0.481
1.767LeuLys: 1.767 ± 0.323
4.065LeuLeu: 4.065 ± 0.535
1.885LeuMet: 1.885 ± 0.377
2.121LeuAsn: 2.121 ± 0.32
4.831LeuPro: 4.831 ± 0.552
1.473LeuGln: 1.473 ± 0.294
5.243LeuArg: 5.243 ± 0.588
4.713LeuSer: 4.713 ± 0.539
5.302LeuThr: 5.302 ± 0.675
6.539LeuVal: 6.539 ± 0.634
1.885LeuTrp: 1.885 ± 0.361
1.296LeuTyr: 1.296 ± 0.192
0.0LeuXaa: 0.0 ± 0.0
Met
3.594MetAla: 3.594 ± 0.437
0.236MetCys: 0.236 ± 0.105
0.884MetAsp: 0.884 ± 0.217
0.766MetGlu: 0.766 ± 0.202
0.648MetPhe: 0.648 ± 0.183
1.649MetGly: 1.649 ± 0.314
0.53MetHis: 0.53 ± 0.154
1.06MetIle: 1.06 ± 0.25
0.53MetLys: 0.53 ± 0.167
1.355MetLeu: 1.355 ± 0.301
0.295MetMet: 0.295 ± 0.162
0.471MetAsn: 0.471 ± 0.158
1.708MetPro: 1.708 ± 0.304
0.707MetGln: 0.707 ± 0.26
1.708MetArg: 1.708 ± 0.461
1.649MetSer: 1.649 ± 0.284
3.004MetThr: 3.004 ± 0.357
0.766MetVal: 0.766 ± 0.24
0.53MetTrp: 0.53 ± 0.182
0.236MetTyr: 0.236 ± 0.096
0.0MetXaa: 0.0 ± 0.0
Asn
2.946AsnAla: 2.946 ± 0.403
0.236AsnCys: 0.236 ± 0.111
1.885AsnAsp: 1.885 ± 0.314
1.001AsnGlu: 1.001 ± 0.191
0.53AsnPhe: 0.53 ± 0.212
3.476AsnGly: 3.476 ± 0.454
0.884AsnHis: 0.884 ± 0.272
1.001AsnIle: 1.001 ± 0.318
0.707AsnLys: 0.707 ± 0.195
1.826AsnLeu: 1.826 ± 0.306
0.53AsnMet: 0.53 ± 0.171
0.943AsnAsn: 0.943 ± 0.217
3.004AsnPro: 3.004 ± 0.398
0.825AsnGln: 0.825 ± 0.206
2.062AsnArg: 2.062 ± 0.442
2.062AsnSer: 2.062 ± 0.318
2.239AsnThr: 2.239 ± 0.467
1.944AsnVal: 1.944 ± 0.311
0.53AsnTrp: 0.53 ± 0.147
0.825AsnTyr: 0.825 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
7.482ProAla: 7.482 ± 0.832
0.825ProCys: 0.825 ± 0.263
5.302ProAsp: 5.302 ± 0.621
3.829ProGlu: 3.829 ± 0.452
1.414ProPhe: 1.414 ± 0.292
5.42ProGly: 5.42 ± 0.56
1.06ProHis: 1.06 ± 0.246
3.004ProIle: 3.004 ± 0.38
2.474ProLys: 2.474 ± 0.358
3.476ProLeu: 3.476 ± 0.476
1.649ProMet: 1.649 ± 0.354
2.121ProAsn: 2.121 ± 0.287
3.181ProPro: 3.181 ± 0.53
1.885ProGln: 1.885 ± 0.298
3.888ProArg: 3.888 ± 0.501
3.181ProSer: 3.181 ± 0.384
4.124ProThr: 4.124 ± 0.542
3.652ProVal: 3.652 ± 0.385
1.296ProTrp: 1.296 ± 0.293
1.237ProTyr: 1.237 ± 0.289
0.0ProXaa: 0.0 ± 0.0
Gln
3.535GlnAla: 3.535 ± 0.456
0.177GlnCys: 0.177 ± 0.099
1.001GlnAsp: 1.001 ± 0.241
1.178GlnGlu: 1.178 ± 0.322
1.119GlnPhe: 1.119 ± 0.273
2.297GlnGly: 2.297 ± 0.424
0.943GlnHis: 0.943 ± 0.258
1.826GlnIle: 1.826 ± 0.318
0.648GlnLys: 0.648 ± 0.161
3.24GlnLeu: 3.24 ± 0.509
0.825GlnMet: 0.825 ± 0.25
0.707GlnAsn: 0.707 ± 0.138
1.767GlnPro: 1.767 ± 0.39
1.885GlnGln: 1.885 ± 0.352
3.181GlnArg: 3.181 ± 0.38
2.239GlnSer: 2.239 ± 0.379
2.062GlnThr: 2.062 ± 0.384
2.474GlnVal: 2.474 ± 0.38
1.06GlnTrp: 1.06 ± 0.277
1.355GlnTyr: 1.355 ± 0.282
0.0GlnXaa: 0.0 ± 0.0
Arg
8.189ArgAla: 8.189 ± 0.736
1.237ArgCys: 1.237 ± 0.285
5.42ArgAsp: 5.42 ± 0.529
4.477ArgGlu: 4.477 ± 0.57
2.062ArgPhe: 2.062 ± 0.346
6.598ArgGly: 6.598 ± 0.621
2.415ArgHis: 2.415 ± 0.409
3.535ArgIle: 3.535 ± 0.424
2.297ArgLys: 2.297 ± 0.317
5.361ArgLeu: 5.361 ± 0.469
2.533ArgMet: 2.533 ± 0.33
2.592ArgAsn: 2.592 ± 0.389
3.652ArgPro: 3.652 ± 0.427
2.651ArgGln: 2.651 ± 0.421
7.128ArgArg: 7.128 ± 0.893
3.594ArgSer: 3.594 ± 0.462
3.652ArgThr: 3.652 ± 0.481
4.948ArgVal: 4.948 ± 0.585
1.414ArgTrp: 1.414 ± 0.292
1.473ArgTyr: 1.473 ± 0.321
0.0ArgXaa: 0.0 ± 0.0
Ser
5.479SerAla: 5.479 ± 0.826
0.295SerCys: 0.295 ± 0.165
3.535SerAsp: 3.535 ± 0.425
3.299SerGlu: 3.299 ± 0.539
1.119SerPhe: 1.119 ± 0.274
5.538SerGly: 5.538 ± 0.603
0.825SerHis: 0.825 ± 0.234
2.769SerIle: 2.769 ± 0.473
1.885SerLys: 1.885 ± 0.321
3.181SerLeu: 3.181 ± 0.432
1.296SerMet: 1.296 ± 0.216
2.062SerAsn: 2.062 ± 0.364
2.828SerPro: 2.828 ± 0.379
1.355SerGln: 1.355 ± 0.299
3.888SerArg: 3.888 ± 0.607
2.356SerSer: 2.356 ± 0.409
4.183SerThr: 4.183 ± 0.441
3.947SerVal: 3.947 ± 0.522
1.355SerTrp: 1.355 ± 0.264
1.001SerTyr: 1.001 ± 0.263
0.0SerXaa: 0.0 ± 0.0
Thr
6.951ThrAla: 6.951 ± 0.531
0.471ThrCys: 0.471 ± 0.198
4.654ThrAsp: 4.654 ± 0.578
4.124ThrGlu: 4.124 ± 0.41
1.944ThrPhe: 1.944 ± 0.406
5.655ThrGly: 5.655 ± 0.667
1.532ThrHis: 1.532 ± 0.27
2.946ThrIle: 2.946 ± 0.395
2.356ThrLys: 2.356 ± 0.432
5.243ThrLeu: 5.243 ± 0.578
1.296ThrMet: 1.296 ± 0.248
2.062ThrAsn: 2.062 ± 0.413
4.831ThrPro: 4.831 ± 0.591
2.18ThrGln: 2.18 ± 0.376
4.713ThrArg: 4.713 ± 0.582
3.417ThrSer: 3.417 ± 0.366
4.3ThrThr: 4.3 ± 0.566
5.655ThrVal: 5.655 ± 0.53
1.296ThrTrp: 1.296 ± 0.222
1.178ThrTyr: 1.178 ± 0.293
0.0ThrXaa: 0.0 ± 0.0
Val
8.837ValAla: 8.837 ± 0.845
1.237ValCys: 1.237 ± 0.299
5.891ValAsp: 5.891 ± 0.582
5.832ValGlu: 5.832 ± 0.595
1.649ValPhe: 1.649 ± 0.329
5.538ValGly: 5.538 ± 0.612
1.473ValHis: 1.473 ± 0.26
3.299ValIle: 3.299 ± 0.389
2.592ValLys: 2.592 ± 0.374
4.948ValLeu: 4.948 ± 0.52
1.885ValMet: 1.885 ± 0.425
1.826ValAsn: 1.826 ± 0.34
4.242ValPro: 4.242 ± 0.509
2.769ValGln: 2.769 ± 0.326
5.891ValArg: 5.891 ± 0.665
3.535ValSer: 3.535 ± 0.483
5.596ValThr: 5.596 ± 0.693
7.128ValVal: 7.128 ± 0.683
1.767ValTrp: 1.767 ± 0.287
1.885ValTyr: 1.885 ± 0.267
0.0ValXaa: 0.0 ± 0.0
Trp
1.885TrpAla: 1.885 ± 0.358
0.412TrpCys: 0.412 ± 0.144
1.178TrpAsp: 1.178 ± 0.301
0.766TrpGlu: 0.766 ± 0.224
0.53TrpPhe: 0.53 ± 0.231
1.001TrpGly: 1.001 ± 0.226
0.648TrpHis: 0.648 ± 0.238
0.766TrpIle: 0.766 ± 0.229
0.884TrpLys: 0.884 ± 0.2
2.003TrpLeu: 2.003 ± 0.365
0.589TrpMet: 0.589 ± 0.212
0.707TrpAsn: 0.707 ± 0.28
1.178TrpPro: 1.178 ± 0.256
0.766TrpGln: 0.766 ± 0.204
1.708TrpArg: 1.708 ± 0.311
1.237TrpSer: 1.237 ± 0.312
1.649TrpThr: 1.649 ± 0.263
1.767TrpVal: 1.767 ± 0.27
0.353TrpTrp: 0.353 ± 0.117
0.589TrpTyr: 0.589 ± 0.182
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.003TyrAla: 2.003 ± 0.333
0.295TyrCys: 0.295 ± 0.144
1.473TyrAsp: 1.473 ± 0.295
1.708TyrGlu: 1.708 ± 0.394
0.471TyrPhe: 0.471 ± 0.188
2.18TyrGly: 2.18 ± 0.382
0.707TyrHis: 0.707 ± 0.215
0.766TyrIle: 0.766 ± 0.248
0.648TyrLys: 0.648 ± 0.181
1.355TyrLeu: 1.355 ± 0.257
0.353TyrMet: 0.353 ± 0.146
0.825TyrAsn: 0.825 ± 0.201
1.178TyrPro: 1.178 ± 0.254
0.648TyrGln: 0.648 ± 0.231
2.18TyrArg: 2.18 ± 0.411
1.237TyrSer: 1.237 ± 0.294
1.826TyrThr: 1.826 ± 0.286
1.944TyrVal: 1.944 ± 0.346
0.471TyrTrp: 0.471 ± 0.181
0.589TyrTyr: 0.589 ± 0.216
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (16976 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski