Paenirhodobacter enshiensis
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2781 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A086XZQ0|A0A086XZQ0_9RHOB Lipopolysaccharide export system permease protein LptF OS=Paenirhodobacter enshiensis OX=1105367 GN=CG50_15935 PE=4 SV=1
MM1 pKa = 7.5 TEE3 pKa = 4.31 GAPLIRR9 pKa = 11.84 PSTTEE14 pKa = 3.49 HH15 pKa = 6.87 PLYY18 pKa = 10.96 DD19 pKa = 4.01 SVADD23 pKa = 3.54 ACRR26 pKa = 11.84 TVFDD30 pKa = 4.62 PEE32 pKa = 4.2 IPVNIYY38 pKa = 10.8 DD39 pKa = 4.42 LGLIYY44 pKa = 10.24 TIAISDD50 pKa = 4.07 DD51 pKa = 3.67 DD52 pKa = 4.04 VVNIVMTLTAPGCPVAGDD70 pKa = 3.58 MPGWVQNAVEE80 pKa = 4.39 SVPGVRR86 pKa = 11.84 EE87 pKa = 4.04 VNVEE91 pKa = 4.01 MTFDD95 pKa = 4.09 PQWGMDD101 pKa = 3.61 MMSDD105 pKa = 3.68 EE106 pKa = 4.89 ARR108 pKa = 11.84 LEE110 pKa = 4.14 LGFMM114 pKa = 4.54
Molecular weight: 12.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.681
IPC_protein 3.63
Toseland 3.427
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.579
Rodwell 3.465
Grimsley 3.338
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 3.961
Thurlkill 3.49
EMBOSS 3.592
Sillero 3.757
Patrickios 1.837
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.723
Protein with the highest isoelectric point:
>tr|A0A086XQG0|A0A086XQG0_9RHOB DNA repair photolyase OS=Paenirhodobacter enshiensis OX=1105367 GN=CG50_11015 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.38 RR3 pKa = 11.84 TYY5 pKa = 10.3 QPSQLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.38 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.43 GGRR28 pKa = 11.84 KK29 pKa = 8.99 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.97 VLSAA44 pKa = 4.11
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.429
IPC2_protein 11.111
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.31
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.047
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2781
0
2781
881810
41
2893
317.1
34.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.32 ± 0.072
0.846 ± 0.016
5.662 ± 0.035
5.758 ± 0.042
3.569 ± 0.03
8.97 ± 0.048
1.97 ± 0.022
4.995 ± 0.032
2.972 ± 0.041
10.257 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.736 ± 0.024
2.224 ± 0.024
5.411 ± 0.039
2.836 ± 0.022
7.321 ± 0.048
4.949 ± 0.03
5.389 ± 0.03
7.34 ± 0.044
1.371 ± 0.018
2.102 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here