Chelatococcus sp. CO-6
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4557 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K2DBJ6|A0A0K2DBJ6_9RHIZ CoA-transferase OS=Chelatococcus sp. CO-6 OX=1702325 GN=AL346_08080 PE=4 SV=1
MM1 pKa = 7.47 TYY3 pKa = 10.72 VVTDD7 pKa = 3.39 GCIRR11 pKa = 11.84 CKK13 pKa = 10.79 YY14 pKa = 7.94 QDD16 pKa = 3.96 CVEE19 pKa = 4.24 VCPVDD24 pKa = 3.66 CFYY27 pKa = 11.22 EE28 pKa = 4.96 GEE30 pKa = 4.4 TMLVIHH36 pKa = 7.35 PDD38 pKa = 3.09 EE39 pKa = 5.88 CIDD42 pKa = 4.44 CGVCEE47 pKa = 4.63 PEE49 pKa = 4.77 CPADD53 pKa = 4.81 AIVSDD58 pKa = 3.98 TDD60 pKa = 3.28 PRR62 pKa = 11.84 ADD64 pKa = 2.86 RR65 pKa = 11.84 WLPVNAKK72 pKa = 9.72 YY73 pKa = 10.3 AAIWPNITDD82 pKa = 4.11 KK83 pKa = 11.63 GPVPDD88 pKa = 6.09 DD89 pKa = 3.57 ADD91 pKa = 3.19 AWKK94 pKa = 10.46 DD95 pKa = 3.66 VPDD98 pKa = 3.68 KK99 pKa = 11.31 EE100 pKa = 4.63 KK101 pKa = 10.89 FLFSTEE107 pKa = 3.79 KK108 pKa = 9.65 MM109 pKa = 3.71
Molecular weight: 12.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.842
IPC2_protein 4.05
IPC_protein 4.012
Toseland 3.808
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.948
Rodwell 3.846
Grimsley 3.719
Solomon 3.999
Lehninger 3.961
Nozaki 4.126
DTASelect 4.368
Thurlkill 3.859
EMBOSS 3.948
Sillero 4.139
Patrickios 0.782
IPC_peptide 3.999
IPC2_peptide 4.113
IPC2.peptide.svr19 4.024
Protein with the highest isoelectric point:
>tr|A0A0K2DBS7|A0A0K2DBS7_9RHIZ Uncharacterized protein OS=Chelatococcus sp. CO-6 OX=1702325 GN=AL346_08655 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.38 GGRR28 pKa = 11.84 KK29 pKa = 9.28 VIASRR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4557
0
4557
1442732
29
4510
316.6
34.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.917 ± 0.057
0.789 ± 0.011
5.437 ± 0.03
5.828 ± 0.031
3.712 ± 0.022
8.968 ± 0.034
1.957 ± 0.016
4.846 ± 0.03
2.677 ± 0.03
10.529 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.358 ± 0.017
2.151 ± 0.018
5.352 ± 0.029
2.658 ± 0.021
7.781 ± 0.039
4.666 ± 0.024
5.021 ± 0.023
8.006 ± 0.031
1.265 ± 0.013
2.084 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here