Nariva virus
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B8XH61|B8XH61_9MONO Isoform of B8XH59 C protein OS=Nariva virus OX=590647 GN=P/V/C PE=4 SV=1
MM1 pKa = 6.91 EE2 pKa = 4.72 TNGQKK7 pKa = 10.37 VIRR10 pKa = 11.84 DD11 pKa = 3.98 ALQVLAVVKK20 pKa = 9.18 EE21 pKa = 4.31 TDD23 pKa = 3.28 SPTTEE28 pKa = 4.11 NQCGRR33 pKa = 11.84 RR34 pKa = 11.84 LSVQGVTTQTSTTDD48 pKa = 3.42 PEE50 pKa = 4.66 TKK52 pKa = 10.41 GEE54 pKa = 4.16 SGSEE58 pKa = 4.12 EE59 pKa = 3.92 EE60 pKa = 5.62 SGSGWGGEE68 pKa = 4.3 GNSSQEE74 pKa = 3.96 ASGSNQLVDD83 pKa = 3.04 NAEE86 pKa = 4.12 RR87 pKa = 11.84 YY88 pKa = 10.31 GEE90 pKa = 4.46 GEE92 pKa = 4.1 SKK94 pKa = 10.84 GPQGPGGFDD103 pKa = 3.39 GVHH106 pKa = 7.13 DD107 pKa = 4.02 SPVRR111 pKa = 11.84 QDD113 pKa = 4.23 HH114 pKa = 6.68 PDD116 pKa = 3.0 EE117 pKa = 4.96 GIRR120 pKa = 11.84 RR121 pKa = 11.84 EE122 pKa = 3.98 PCYY125 pKa = 10.92 VLLGAASGGAYY136 pKa = 10.21 LSEE139 pKa = 4.56 SEE141 pKa = 5.11 GEE143 pKa = 4.21 GSPTRR148 pKa = 11.84 GSPVGEE154 pKa = 4.17 SGDD157 pKa = 3.66 AKK159 pKa = 10.91 VIAPPNRR166 pKa = 11.84 GSTINDD172 pKa = 3.34 NPSCDD177 pKa = 3.77 DD178 pKa = 3.51 VKK180 pKa = 11.13 EE181 pKa = 4.14 SLGFISQPKK190 pKa = 8.34 PRR192 pKa = 11.84 RR193 pKa = 11.84 LTGIDD198 pKa = 3.15 TSTDD202 pKa = 2.94 TGTPIPPPRR211 pKa = 11.84 DD212 pKa = 3.33 YY213 pKa = 10.88 TKK215 pKa = 9.98 RR216 pKa = 11.84 GHH218 pKa = 5.71 RR219 pKa = 11.84 RR220 pKa = 11.84 EE221 pKa = 4.26 YY222 pKa = 10.64 SLTWTDD228 pKa = 3.37 CGFLVEE234 pKa = 4.63 SWCNPVCARR243 pKa = 11.84 VTPLPRR249 pKa = 11.84 RR250 pKa = 11.84 EE251 pKa = 3.88 SCKK254 pKa = 10.38 CGKK257 pKa = 10.43 CPVFCPEE264 pKa = 4.07 CVGPGVSQEE273 pKa = 5.06 DD274 pKa = 4.41 IYY276 pKa = 11.86 NDD278 pKa = 3.29
Molecular weight: 29.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.66
IPC2_protein 4.698
IPC_protein 4.622
Toseland 4.469
ProMoST 4.673
Dawson 4.558
Bjellqvist 4.724
Wikipedia 4.418
Rodwell 4.457
Grimsley 4.38
Solomon 4.558
Lehninger 4.507
Nozaki 4.673
DTASelect 4.813
Thurlkill 4.469
EMBOSS 4.444
Sillero 4.736
Patrickios 3.77
IPC_peptide 4.571
IPC2_peptide 4.724
IPC2.peptide.svr19 4.699
Protein with the highest isoelectric point:
>tr|B8XH61|B8XH61_9MONO Isoform of B8XH59 C protein OS=Nariva virus OX=590647 GN=P/V/C PE=4 SV=1
MM1 pKa = 8.09 PSRR4 pKa = 11.84 FLQSLRR10 pKa = 11.84 KK11 pKa = 8.48 LTLRR15 pKa = 11.84 RR16 pKa = 11.84 QRR18 pKa = 11.84 TNAEE22 pKa = 3.72 EE23 pKa = 4.52 DD24 pKa = 3.65 SQSRR28 pKa = 11.84 EE29 pKa = 4.17 SPPRR33 pKa = 11.84 PPPRR37 pKa = 11.84 TPRR40 pKa = 11.84 PRR42 pKa = 11.84 VRR44 pKa = 11.84 VGVRR48 pKa = 11.84 KK49 pKa = 10.11 NPDD52 pKa = 2.93 LVGVEE57 pKa = 4.93 KK58 pKa = 9.73 EE59 pKa = 4.19 TQARR63 pKa = 11.84 KK64 pKa = 8.44 QAEE67 pKa = 4.73 AINLLTMLRR76 pKa = 11.84 DD77 pKa = 3.6 MEE79 pKa = 4.37 RR80 pKa = 11.84 EE81 pKa = 3.71 NLRR84 pKa = 11.84 GLRR87 pKa = 11.84 GLEE90 pKa = 3.76 GLTEE94 pKa = 4.05 YY95 pKa = 8.12 TTVQFVKK102 pKa = 9.61 TILMRR107 pKa = 11.84 VSEE110 pKa = 4.45 GSPVTSCWVQQVEE123 pKa = 4.1 EE124 pKa = 5.36 HH125 pKa = 6.33 ICQSQRR131 pKa = 11.84 EE132 pKa = 4.23 KK133 pKa = 10.94 EE134 pKa = 4.19 ALHH137 pKa = 6.7 EE138 pKa = 4.1 AVQWVRR144 pKa = 11.84 AVMQKK149 pKa = 10.74
Molecular weight: 17.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.252
IPC2_protein 9.209
IPC_protein 9.502
Toseland 10.467
ProMoST 10.116
Dawson 10.526
Bjellqvist 10.175
Wikipedia 10.687
Rodwell 10.76
Grimsley 10.555
Solomon 10.643
Lehninger 10.613
Nozaki 10.467
DTASelect 10.16
Thurlkill 10.452
EMBOSS 10.862
Sillero 10.467
Patrickios 10.54
IPC_peptide 10.643
IPC2_peptide 8.99
IPC2.peptide.svr19 8.407
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
5226
149
2201
653.3
72.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.487 ± 0.542
1.933 ± 0.25
5.549 ± 0.317
5.434 ± 0.481
2.832 ± 0.401
6.525 ± 0.779
2.354 ± 0.479
7.08 ± 0.795
4.363 ± 0.26
9.127 ± 0.656
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.698 ± 0.354
4.497 ± 0.259
5.683 ± 0.612
4.286 ± 0.484
6.008 ± 0.317
7.597 ± 0.515
6.142 ± 0.364
6.774 ± 0.368
1.11 ± 0.169
3.521 ± 0.402
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here