Microbacterium phage Triscuit

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4A1T4|A0A2R4A1T4_9CAUD Uncharacterized protein OS=Microbacterium phage Triscuit OX=2126721 GN=91 PE=4 SV=1
MM1 pKa = 7.62AIEE4 pKa = 4.88APTPSKK10 pKa = 10.02EE11 pKa = 4.26HH12 pKa = 5.85FWVEE16 pKa = 3.8LKK18 pKa = 10.52YY19 pKa = 11.23VPVTVEE25 pKa = 3.88EE26 pKa = 4.4QEE28 pKa = 4.63DD29 pKa = 3.96EE30 pKa = 4.32SLSIFASEE38 pKa = 4.14EE39 pKa = 3.72ALEE42 pKa = 4.5ASDD45 pKa = 5.51DD46 pKa = 3.9EE47 pKa = 4.73TVYY50 pKa = 11.23GCWFCHH56 pKa = 6.0TPLNTDD62 pKa = 3.91TFPTEE67 pKa = 4.4CRR69 pKa = 11.84PSDD72 pKa = 3.23

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4A1P6|A0A2R4A1P6_9CAUD Uncharacterized protein OS=Microbacterium phage Triscuit OX=2126721 GN=47 PE=4 SV=1
MM1 pKa = 7.78CILRR5 pKa = 11.84RR6 pKa = 11.84YY7 pKa = 8.13NRR9 pKa = 11.84SMTDD13 pKa = 2.72QTNADD18 pKa = 4.08NIPRR22 pKa = 11.84YY23 pKa = 9.96ARR25 pKa = 11.84YY26 pKa = 7.94QGKK29 pKa = 8.21RR30 pKa = 11.84VRR32 pKa = 11.84VIDD35 pKa = 3.92YY36 pKa = 10.25QGEE39 pKa = 4.89GYY41 pKa = 10.38FRR43 pKa = 11.84ILLPNDD49 pKa = 2.53TMTNVKK55 pKa = 10.02RR56 pKa = 11.84EE57 pKa = 3.66RR58 pKa = 11.84LTFVRR63 pKa = 11.84AKK65 pKa = 10.44KK66 pKa = 10.15KK67 pKa = 10.51

Molecular weight: 8.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

19593

35

1018

188.4

21.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.177 ± 0.338

0.72 ± 0.109

6.599 ± 0.319

7.125 ± 0.453

3.399 ± 0.139

7.727 ± 0.309

1.934 ± 0.147

5.482 ± 0.285

5.226 ± 0.318

8.345 ± 0.437

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.736 ± 0.127

3.966 ± 0.213

4.91 ± 0.195

3.762 ± 0.151

6.16 ± 0.323

5.303 ± 0.229

6.206 ± 0.271

6.262 ± 0.244

1.878 ± 0.142

3.083 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski