Amino acid dipepetide frequency for Microbacterium phage Triscuit

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.106AlaAla: 10.106 ± 0.964
0.357AlaCys: 0.357 ± 0.148
5.666AlaAsp: 5.666 ± 0.621
7.197AlaGlu: 7.197 ± 0.723
2.297AlaPhe: 2.297 ± 0.359
7.095AlaGly: 7.095 ± 0.877
1.378AlaHis: 1.378 ± 0.224
5.972AlaIle: 5.972 ± 0.752
5.461AlaLys: 5.461 ± 0.611
8.575AlaLeu: 8.575 ± 0.944
2.246AlaMet: 2.246 ± 0.297
3.165AlaAsn: 3.165 ± 0.468
3.522AlaPro: 3.522 ± 0.444
2.96AlaGln: 2.96 ± 0.327
6.686AlaArg: 6.686 ± 0.676
4.594AlaSer: 4.594 ± 0.67
5.206AlaThr: 5.206 ± 0.607
5.155AlaVal: 5.155 ± 0.648
1.633AlaTrp: 1.633 ± 0.33
2.45AlaTyr: 2.45 ± 0.349
0.0AlaXaa: 0.0 ± 0.0
Cys
0.51CysAla: 0.51 ± 0.193
0.051CysCys: 0.051 ± 0.056
0.612CysAsp: 0.612 ± 0.213
0.357CysGlu: 0.357 ± 0.123
0.102CysPhe: 0.102 ± 0.066
0.868CysGly: 0.868 ± 0.25
0.306CysHis: 0.306 ± 0.126
0.204CysIle: 0.204 ± 0.106
0.408CysLys: 0.408 ± 0.144
0.612CysLeu: 0.612 ± 0.203
0.255CysMet: 0.255 ± 0.091
0.51CysAsn: 0.51 ± 0.191
0.408CysPro: 0.408 ± 0.16
0.153CysGln: 0.153 ± 0.09
0.459CysArg: 0.459 ± 0.149
0.408CysSer: 0.408 ± 0.163
0.357CysThr: 0.357 ± 0.13
0.255CysVal: 0.255 ± 0.136
0.153CysTrp: 0.153 ± 0.092
0.204CysTyr: 0.204 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
5.615AspAla: 5.615 ± 0.64
0.153AspCys: 0.153 ± 0.086
5.921AspAsp: 5.921 ± 1.004
6.891AspGlu: 6.891 ± 1.206
2.705AspPhe: 2.705 ± 0.431
4.543AspGly: 4.543 ± 0.482
0.868AspHis: 0.868 ± 0.225
3.777AspIle: 3.777 ± 0.436
2.756AspLys: 2.756 ± 0.391
5.717AspLeu: 5.717 ± 0.591
2.501AspMet: 2.501 ± 0.333
2.501AspAsn: 2.501 ± 0.34
3.777AspPro: 3.777 ± 0.423
2.195AspGln: 2.195 ± 0.276
3.675AspArg: 3.675 ± 0.531
2.654AspSer: 2.654 ± 0.312
3.216AspThr: 3.216 ± 0.378
3.522AspVal: 3.522 ± 0.485
1.072AspTrp: 1.072 ± 0.26
1.94AspTyr: 1.94 ± 0.365
0.0AspXaa: 0.0 ± 0.0
Glu
7.35GluAla: 7.35 ± 0.639
0.459GluCys: 0.459 ± 0.149
5.768GluAsp: 5.768 ± 0.96
7.044GluGlu: 7.044 ± 1.143
2.858GluPhe: 2.858 ± 0.358
4.236GluGly: 4.236 ± 0.434
1.531GluHis: 1.531 ± 0.288
3.267GluIle: 3.267 ± 0.391
3.522GluLys: 3.522 ± 0.521
7.452GluLeu: 7.452 ± 0.783
2.399GluMet: 2.399 ± 0.308
2.297GluAsn: 2.297 ± 0.267
2.144GluPro: 2.144 ± 0.338
2.552GluGln: 2.552 ± 0.362
3.42GluArg: 3.42 ± 0.419
3.114GluSer: 3.114 ± 0.465
3.471GluThr: 3.471 ± 0.471
4.798GluVal: 4.798 ± 0.63
1.378GluTrp: 1.378 ± 0.309
2.195GluTyr: 2.195 ± 0.297
0.0GluXaa: 0.0 ± 0.0
Phe
2.246PheAla: 2.246 ± 0.32
0.204PheCys: 0.204 ± 0.12
2.858PheAsp: 2.858 ± 0.313
2.144PheGlu: 2.144 ± 0.328
1.327PhePhe: 1.327 ± 0.223
2.552PheGly: 2.552 ± 0.411
0.561PheHis: 0.561 ± 0.151
2.042PheIle: 2.042 ± 0.267
1.94PheLys: 1.94 ± 0.315
2.45PheLeu: 2.45 ± 0.31
0.817PheMet: 0.817 ± 0.21
1.174PheAsn: 1.174 ± 0.251
1.735PhePro: 1.735 ± 0.293
1.021PheGln: 1.021 ± 0.237
2.093PheArg: 2.093 ± 0.363
2.45PheSer: 2.45 ± 0.351
2.705PheThr: 2.705 ± 0.384
1.837PheVal: 1.837 ± 0.392
0.408PheTrp: 0.408 ± 0.156
1.429PheTyr: 1.429 ± 0.294
0.0PheXaa: 0.0 ± 0.0
Gly
6.635GlyAla: 6.635 ± 0.94
0.306GlyCys: 0.306 ± 0.137
3.726GlyAsp: 3.726 ± 0.536
4.492GlyGlu: 4.492 ± 0.445
2.705GlyPhe: 2.705 ± 0.389
6.278GlyGly: 6.278 ± 0.693
1.429GlyHis: 1.429 ± 0.283
3.318GlyIle: 3.318 ± 0.514
5.615GlyLys: 5.615 ± 0.567
6.023GlyLeu: 6.023 ± 0.883
2.45GlyMet: 2.45 ± 0.403
3.216GlyAsn: 3.216 ± 0.393
3.675GlyPro: 3.675 ± 0.438
2.705GlyGln: 2.705 ± 0.441
4.696GlyArg: 4.696 ± 0.607
4.39GlySer: 4.39 ± 0.556
5.257GlyThr: 5.257 ± 0.725
5.87GlyVal: 5.87 ± 0.551
1.735GlyTrp: 1.735 ± 0.344
2.756GlyTyr: 2.756 ± 0.49
0.0GlyXaa: 0.0 ± 0.0
His
1.072HisAla: 1.072 ± 0.289
0.357HisCys: 0.357 ± 0.176
1.327HisAsp: 1.327 ± 0.299
1.021HisGlu: 1.021 ± 0.283
1.021HisPhe: 1.021 ± 0.242
1.582HisGly: 1.582 ± 0.298
0.357HisHis: 0.357 ± 0.129
1.276HisIle: 1.276 ± 0.271
0.612HisLys: 0.612 ± 0.187
1.531HisLeu: 1.531 ± 0.263
0.664HisMet: 0.664 ± 0.189
0.715HisAsn: 0.715 ± 0.189
1.225HisPro: 1.225 ± 0.28
0.664HisGln: 0.664 ± 0.173
1.378HisArg: 1.378 ± 0.222
0.766HisSer: 0.766 ± 0.197
1.582HisThr: 1.582 ± 0.261
1.123HisVal: 1.123 ± 0.227
0.255HisTrp: 0.255 ± 0.116
0.817HisTyr: 0.817 ± 0.209
0.0HisXaa: 0.0 ± 0.0
Ile
5.002IleAla: 5.002 ± 0.614
0.204IleCys: 0.204 ± 0.093
4.492IleAsp: 4.492 ± 0.485
5.053IleGlu: 5.053 ± 0.491
1.582IlePhe: 1.582 ± 0.306
4.083IleGly: 4.083 ± 0.601
1.123IleHis: 1.123 ± 0.264
2.807IleIle: 2.807 ± 0.636
2.195IleLys: 2.195 ± 0.376
4.032IleLeu: 4.032 ± 0.744
0.97IleMet: 0.97 ± 0.256
2.45IleAsn: 2.45 ± 0.319
3.369IlePro: 3.369 ± 0.494
1.786IleGln: 1.786 ± 0.385
3.675IleArg: 3.675 ± 0.485
2.654IleSer: 2.654 ± 0.39
3.165IleThr: 3.165 ± 0.435
3.726IleVal: 3.726 ± 0.384
1.072IleTrp: 1.072 ± 0.222
1.378IleTyr: 1.378 ± 0.345
0.0IleXaa: 0.0 ± 0.0
Lys
5.768LysAla: 5.768 ± 0.598
0.459LysCys: 0.459 ± 0.171
3.318LysAsp: 3.318 ± 0.423
2.96LysGlu: 2.96 ± 0.451
1.735LysPhe: 1.735 ± 0.33
3.471LysGly: 3.471 ± 0.376
0.97LysHis: 0.97 ± 0.23
2.246LysIle: 2.246 ± 0.303
3.471LysLys: 3.471 ± 0.514
4.747LysLeu: 4.747 ± 0.48
1.021LysMet: 1.021 ± 0.183
1.633LysAsn: 1.633 ± 0.288
2.705LysPro: 2.705 ± 0.47
1.991LysGln: 1.991 ± 0.367
3.522LysArg: 3.522 ± 0.571
2.603LysSer: 2.603 ± 0.485
3.318LysThr: 3.318 ± 0.487
3.471LysVal: 3.471 ± 0.38
0.766LysTrp: 0.766 ± 0.189
2.093LysTyr: 2.093 ± 0.32
0.0LysXaa: 0.0 ± 0.0
Leu
7.656LeuAla: 7.656 ± 0.791
0.408LeuCys: 0.408 ± 0.14
5.717LeuAsp: 5.717 ± 0.741
5.819LeuGlu: 5.819 ± 0.503
2.807LeuPhe: 2.807 ± 0.45
6.329LeuGly: 6.329 ± 0.614
1.378LeuHis: 1.378 ± 0.317
6.023LeuIle: 6.023 ± 0.875
4.798LeuLys: 4.798 ± 0.503
7.401LeuLeu: 7.401 ± 0.9
2.093LeuMet: 2.093 ± 0.3
3.624LeuAsn: 3.624 ± 0.451
4.39LeuPro: 4.39 ± 0.352
2.96LeuGln: 2.96 ± 0.405
4.951LeuArg: 4.951 ± 0.551
4.032LeuSer: 4.032 ± 0.409
5.359LeuThr: 5.359 ± 0.619
4.543LeuVal: 4.543 ± 0.519
1.225LeuTrp: 1.225 ± 0.32
1.94LeuTyr: 1.94 ± 0.38
0.0LeuXaa: 0.0 ± 0.0
Met
3.369MetAla: 3.369 ± 0.4
0.306MetCys: 0.306 ± 0.123
1.786MetAsp: 1.786 ± 0.316
1.276MetGlu: 1.276 ± 0.303
0.612MetPhe: 0.612 ± 0.179
1.48MetGly: 1.48 ± 0.291
0.306MetHis: 0.306 ± 0.117
1.582MetIle: 1.582 ± 0.271
1.48MetLys: 1.48 ± 0.279
1.94MetLeu: 1.94 ± 0.36
0.919MetMet: 0.919 ± 0.202
1.021MetAsn: 1.021 ± 0.225
2.195MetPro: 2.195 ± 0.384
0.919MetGln: 0.919 ± 0.188
1.786MetArg: 1.786 ± 0.344
1.735MetSer: 1.735 ± 0.317
2.246MetThr: 2.246 ± 0.327
1.889MetVal: 1.889 ± 0.295
0.204MetTrp: 0.204 ± 0.098
0.306MetTyr: 0.306 ± 0.125
0.0MetXaa: 0.0 ± 0.0
Asn
3.573AsnAla: 3.573 ± 0.511
0.153AsnCys: 0.153 ± 0.087
1.633AsnAsp: 1.633 ± 0.247
3.011AsnGlu: 3.011 ± 0.425
1.633AsnPhe: 1.633 ± 0.289
4.032AsnGly: 4.032 ± 0.554
0.97AsnHis: 0.97 ± 0.248
1.786AsnIle: 1.786 ± 0.306
1.582AsnLys: 1.582 ± 0.263
2.756AsnLeu: 2.756 ± 0.369
0.919AsnMet: 0.919 ± 0.251
1.429AsnAsn: 1.429 ± 0.306
2.807AsnPro: 2.807 ± 0.375
1.735AsnGln: 1.735 ± 0.326
2.807AsnArg: 2.807 ± 0.432
2.348AsnSer: 2.348 ± 0.342
2.348AsnThr: 2.348 ± 0.387
2.093AsnVal: 2.093 ± 0.32
1.021AsnTrp: 1.021 ± 0.211
1.021AsnTyr: 1.021 ± 0.293
0.0AsnXaa: 0.0 ± 0.0
Pro
4.645ProAla: 4.645 ± 0.508
0.357ProCys: 0.357 ± 0.154
3.318ProAsp: 3.318 ± 0.417
3.726ProGlu: 3.726 ± 0.45
1.174ProPhe: 1.174 ± 0.25
3.879ProGly: 3.879 ± 0.533
1.276ProHis: 1.276 ± 0.225
2.093ProIle: 2.093 ± 0.421
2.858ProLys: 2.858 ± 0.416
3.011ProLeu: 3.011 ± 0.318
1.429ProMet: 1.429 ± 0.315
1.94ProAsn: 1.94 ± 0.371
2.348ProPro: 2.348 ± 0.398
2.399ProGln: 2.399 ± 0.31
2.603ProArg: 2.603 ± 0.484
3.267ProSer: 3.267 ± 0.408
3.879ProThr: 3.879 ± 0.394
2.756ProVal: 2.756 ± 0.368
0.868ProTrp: 0.868 ± 0.233
1.276ProTyr: 1.276 ± 0.281
0.0ProXaa: 0.0 ± 0.0
Gln
2.858GlnAla: 2.858 ± 0.479
0.204GlnCys: 0.204 ± 0.096
1.889GlnAsp: 1.889 ± 0.291
1.786GlnGlu: 1.786 ± 0.376
1.735GlnPhe: 1.735 ± 0.304
2.807GlnGly: 2.807 ± 0.413
0.715GlnHis: 0.715 ± 0.192
2.552GlnIle: 2.552 ± 0.274
1.48GlnLys: 1.48 ± 0.339
3.981GlnLeu: 3.981 ± 0.629
1.327GlnMet: 1.327 ± 0.319
1.582GlnAsn: 1.582 ± 0.311
1.531GlnPro: 1.531 ± 0.317
1.735GlnGln: 1.735 ± 0.307
2.348GlnArg: 2.348 ± 0.42
2.144GlnSer: 2.144 ± 0.381
1.94GlnThr: 1.94 ± 0.324
2.552GlnVal: 2.552 ± 0.326
1.123GlnTrp: 1.123 ± 0.212
1.327GlnTyr: 1.327 ± 0.274
0.0GlnXaa: 0.0 ± 0.0
Arg
4.696ArgAla: 4.696 ± 0.496
0.766ArgCys: 0.766 ± 0.242
2.96ArgAsp: 2.96 ± 0.412
4.543ArgGlu: 4.543 ± 0.582
1.582ArgPhe: 1.582 ± 0.244
5.308ArgGly: 5.308 ± 0.481
1.327ArgHis: 1.327 ± 0.277
3.777ArgIle: 3.777 ± 0.589
3.165ArgLys: 3.165 ± 0.443
5.41ArgLeu: 5.41 ± 0.491
1.735ArgMet: 1.735 ± 0.297
2.654ArgAsn: 2.654 ± 0.408
2.552ArgPro: 2.552 ± 0.403
2.705ArgGln: 2.705 ± 0.395
4.39ArgArg: 4.39 ± 0.63
3.011ArgSer: 3.011 ± 0.395
3.726ArgThr: 3.726 ± 0.472
3.573ArgVal: 3.573 ± 0.458
1.633ArgTrp: 1.633 ± 0.302
2.093ArgTyr: 2.093 ± 0.338
0.0ArgXaa: 0.0 ± 0.0
Ser
4.287SerAla: 4.287 ± 0.627
0.408SerCys: 0.408 ± 0.134
3.624SerAsp: 3.624 ± 0.445
3.42SerGlu: 3.42 ± 0.421
1.684SerPhe: 1.684 ± 0.336
5.002SerGly: 5.002 ± 0.787
1.225SerHis: 1.225 ± 0.264
2.705SerIle: 2.705 ± 0.42
2.042SerLys: 2.042 ± 0.372
4.083SerLeu: 4.083 ± 0.431
1.735SerMet: 1.735 ± 0.311
1.94SerAsn: 1.94 ± 0.298
2.603SerPro: 2.603 ± 0.433
1.94SerGln: 1.94 ± 0.257
3.062SerArg: 3.062 ± 0.465
3.471SerSer: 3.471 ± 0.398
3.726SerThr: 3.726 ± 0.584
3.114SerVal: 3.114 ± 0.414
0.715SerTrp: 0.715 ± 0.207
2.246SerTyr: 2.246 ± 0.367
0.0SerXaa: 0.0 ± 0.0
Thr
6.176ThrAla: 6.176 ± 0.725
0.408ThrCys: 0.408 ± 0.213
3.011ThrAsp: 3.011 ± 0.385
3.93ThrGlu: 3.93 ± 0.431
2.756ThrPhe: 2.756 ± 0.319
5.257ThrGly: 5.257 ± 0.498
1.174ThrHis: 1.174 ± 0.312
3.369ThrIle: 3.369 ± 0.509
3.624ThrLys: 3.624 ± 0.488
5.104ThrLeu: 5.104 ± 0.533
1.225ThrMet: 1.225 ± 0.314
2.246ThrAsn: 2.246 ± 0.375
2.96ThrPro: 2.96 ± 0.486
2.756ThrGln: 2.756 ± 0.362
3.675ThrArg: 3.675 ± 0.555
3.828ThrSer: 3.828 ± 0.517
3.777ThrThr: 3.777 ± 0.521
3.828ThrVal: 3.828 ± 0.488
1.072ThrTrp: 1.072 ± 0.228
1.889ThrTyr: 1.889 ± 0.334
0.0ThrXaa: 0.0 ± 0.0
Val
5.768ValAla: 5.768 ± 0.444
1.174ValCys: 1.174 ± 0.386
4.798ValAsp: 4.798 ± 0.619
3.471ValGlu: 3.471 ± 0.412
1.582ValPhe: 1.582 ± 0.276
4.849ValGly: 4.849 ± 0.488
1.225ValHis: 1.225 ± 0.22
3.471ValIle: 3.471 ± 0.398
2.807ValLys: 2.807 ± 0.391
4.594ValLeu: 4.594 ± 0.665
1.531ValMet: 1.531 ± 0.257
3.318ValAsn: 3.318 ± 0.415
2.909ValPro: 2.909 ± 0.409
2.093ValGln: 2.093 ± 0.279
3.522ValArg: 3.522 ± 0.425
3.471ValSer: 3.471 ± 0.4
4.185ValThr: 4.185 ± 0.52
4.849ValVal: 4.849 ± 0.515
1.276ValTrp: 1.276 ± 0.212
1.735ValTyr: 1.735 ± 0.277
0.0ValXaa: 0.0 ± 0.0
Trp
1.837TrpAla: 1.837 ± 0.305
0.153TrpCys: 0.153 ± 0.082
1.276TrpAsp: 1.276 ± 0.211
1.174TrpGlu: 1.174 ± 0.248
0.766TrpPhe: 0.766 ± 0.214
1.174TrpGly: 1.174 ± 0.216
0.51TrpHis: 0.51 ± 0.202
1.225TrpIle: 1.225 ± 0.26
0.766TrpLys: 0.766 ± 0.201
1.582TrpLeu: 1.582 ± 0.297
0.408TrpMet: 0.408 ± 0.16
0.766TrpAsn: 0.766 ± 0.207
0.715TrpPro: 0.715 ± 0.209
0.919TrpGln: 0.919 ± 0.246
0.919TrpArg: 0.919 ± 0.19
0.97TrpSer: 0.97 ± 0.228
0.868TrpThr: 0.868 ± 0.224
1.429TrpVal: 1.429 ± 0.272
0.459TrpTrp: 0.459 ± 0.142
0.868TrpTyr: 0.868 ± 0.207
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.603TyrAla: 2.603 ± 0.295
0.306TyrCys: 0.306 ± 0.137
2.297TyrAsp: 2.297 ± 0.325
1.94TyrGlu: 1.94 ± 0.346
1.327TyrPhe: 1.327 ± 0.224
2.45TyrGly: 2.45 ± 0.414
0.715TyrHis: 0.715 ± 0.184
1.327TyrIle: 1.327 ± 0.327
1.684TyrLys: 1.684 ± 0.256
2.45TyrLeu: 2.45 ± 0.344
0.664TyrMet: 0.664 ± 0.165
1.48TyrAsn: 1.48 ± 0.326
1.531TyrPro: 1.531 ± 0.278
1.429TyrGln: 1.429 ± 0.272
1.94TyrArg: 1.94 ± 0.333
1.123TyrSer: 1.123 ± 0.331
1.735TyrThr: 1.735 ± 0.272
2.246TyrVal: 2.246 ± 0.354
0.715TyrTrp: 0.715 ± 0.194
0.868TyrTyr: 0.868 ± 0.254
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 104 proteins (19593 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski