Microvirga sp. BSC39

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Microvirga; unclassified Microvirga

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4595 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A086MGW7|A0A086MGW7_9RHIZ Uncharacterized protein OS=Microvirga sp. BSC39 OX=1549810 GN=JH26_18060 PE=4 SV=1
MM1 pKa = 7.78AYY3 pKa = 9.49IPLTEE8 pKa = 4.42NNDD11 pKa = 3.28TQVIYY16 pKa = 11.12NVTTDD21 pKa = 3.59DD22 pKa = 3.59SDD24 pKa = 4.43EE25 pKa = 4.27IFHH28 pKa = 6.95GLGGDD33 pKa = 3.53DD34 pKa = 3.51TFLVNTFLVNNYY46 pKa = 9.44VYY48 pKa = 10.59GISISGSDD56 pKa = 3.22SAGFRR61 pKa = 11.84SEE63 pKa = 4.41LQWDD67 pKa = 4.33VVHH70 pKa = 6.52LAVSARR76 pKa = 11.84FNALSFIDD84 pKa = 3.96VLTFTSPNSATTLTYY99 pKa = 10.54SANSSFGVSQVVGSTAGIDD118 pKa = 3.76TIHH121 pKa = 5.98FTSASEE127 pKa = 4.16RR128 pKa = 11.84VNLDD132 pKa = 2.87LSGTVFANWGRR143 pKa = 11.84ANN145 pKa = 3.37

Molecular weight: 15.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A086MF08|A0A086MF08_9RHIZ Uncharacterized protein OS=Microvirga sp. BSC39 OX=1549810 GN=JH26_22855 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38GGRR28 pKa = 11.84KK29 pKa = 9.21VIAARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.11GRR39 pKa = 11.84KK40 pKa = 9.3RR41 pKa = 11.84LSAA44 pKa = 4.01

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4595

0

4595

1331630

41

3178

289.8

31.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.848 ± 0.046

0.769 ± 0.011

5.504 ± 0.031

6.035 ± 0.035

3.676 ± 0.024

8.453 ± 0.042

2.063 ± 0.017

5.288 ± 0.026

3.411 ± 0.031

10.196 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.445 ± 0.018

2.628 ± 0.023

5.195 ± 0.029

3.348 ± 0.026

7.227 ± 0.039

5.537 ± 0.026

5.313 ± 0.028

7.563 ± 0.031

1.305 ± 0.015

2.194 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski