Amino acid dipepetide frequency for Microvirga sp. BSC39

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.131AlaAla: 15.131 ± 0.146	1.053AlaCys: 1.053 ± 0.03	6.077AlaAsp: 6.077 ± 0.072	7.41AlaGlu: 7.41 ± 0.081	4.458AlaPhe: 4.458 ± 0.068	9.862AlaGly: 9.862 ± 0.1	2.217AlaHis: 2.217 ± 0.041	6.316AlaIle: 6.316 ± 0.066	4.317AlaLys: 4.317 ± 0.067	13.031AlaLeu: 13.031 ± 0.142	3.357AlaMet: 3.357 ± 0.057	2.709AlaAsn: 2.709 ± 0.05	5.188AlaPro: 5.188 ± 0.073	4.243AlaGln: 4.243 ± 0.058	8.024AlaArg: 8.024 ± 0.089	6.581AlaSer: 6.581 ± 0.081	5.538AlaThr: 5.538 ± 0.064	8.77AlaVal: 8.77 ± 0.098	1.491AlaTrp: 1.491 ± 0.037	2.711AlaTyr: 2.711 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.818CysAla: 0.818 ± 0.031	0.09CysCys: 0.09 ± 0.009	0.464CysAsp: 0.464 ± 0.017	0.427CysGlu: 0.427 ± 0.019	0.308CysPhe: 0.308 ± 0.013	0.776CysGly: 0.776 ± 0.026	0.237CysHis: 0.237 ± 0.016	0.397CysIle: 0.397 ± 0.019	0.167CysLys: 0.167 ± 0.011	0.78CysLeu: 0.78 ± 0.025	0.144CysMet: 0.144 ± 0.011	0.214CysAsn: 0.214 ± 0.013	0.408CysPro: 0.408 ± 0.018	0.21CysGln: 0.21 ± 0.011	0.638CysArg: 0.638 ± 0.024	0.439CysSer: 0.439 ± 0.018	0.4CysThr: 0.4 ± 0.017	0.516CysVal: 0.516 ± 0.018	0.093CysTrp: 0.093 ± 0.008	0.169CysTyr: 0.169 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	6.204AspAla: 6.204 ± 0.075	0.39AspCys: 0.39 ± 0.017	2.815AspAsp: 2.815 ± 0.052	3.829AspGlu: 3.829 ± 0.051	2.01AspPhe: 2.01 ± 0.043	4.707AspGly: 4.707 ± 0.077	1.192AspHis: 1.192 ± 0.033	2.875AspIle: 2.875 ± 0.051	1.766AspLys: 1.766 ± 0.038	6.174AspLeu: 6.174 ± 0.069	1.25AspMet: 1.25 ± 0.032	1.243AspAsn: 1.243 ± 0.035	3.546AspPro: 3.546 ± 0.052	1.826AspGln: 1.826 ± 0.045	4.316AspArg: 4.316 ± 0.069	1.992AspSer: 1.992 ± 0.037	2.541AspThr: 2.541 ± 0.061	4.2AspVal: 4.2 ± 0.056	0.829AspTrp: 0.829 ± 0.029	1.338AspTyr: 1.338 ± 0.029	0.0AspXaa: 0.0 ± 0.0
Glu	8.426GluAla: 8.426 ± 0.098	0.336GluCys: 0.336 ± 0.018	2.872GluAsp: 2.872 ± 0.049	3.724GluGlu: 3.724 ± 0.055	1.763GluPhe: 1.763 ± 0.037	4.612GluGly: 4.612 ± 0.064	1.313GluHis: 1.313 ± 0.029	3.637GluIle: 3.637 ± 0.053	2.299GluLys: 2.299 ± 0.05	5.366GluLeu: 5.366 ± 0.066	1.539GluMet: 1.539 ± 0.037	1.627GluAsn: 1.627 ± 0.038	2.96GluPro: 2.96 ± 0.05	2.321GluGln: 2.321 ± 0.051	5.643GluArg: 5.643 ± 0.074	2.477GluSer: 2.477 ± 0.044	3.548GluThr: 3.548 ± 0.05	4.16GluVal: 4.16 ± 0.065	0.758GluTrp: 0.758 ± 0.025	0.966GluTyr: 0.966 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	4.156PheAla: 4.156 ± 0.058	0.354PheCys: 0.354 ± 0.016	2.504PheAsp: 2.504 ± 0.044	2.245PheGlu: 2.245 ± 0.044	1.428PhePhe: 1.428 ± 0.04	3.532PheGly: 3.532 ± 0.063	0.703PheHis: 0.703 ± 0.026	1.814PheIle: 1.814 ± 0.041	1.117PheLys: 1.117 ± 0.03	3.425PheLeu: 3.425 ± 0.062	0.888PheMet: 0.888 ± 0.027	1.008PheAsn: 1.008 ± 0.031	1.598PhePro: 1.598 ± 0.034	1.144PheGln: 1.144 ± 0.032	2.285PheArg: 2.285 ± 0.043	2.215PheSer: 2.215 ± 0.045	1.98PheThr: 1.98 ± 0.045	2.867PheVal: 2.867 ± 0.052	0.572PheTrp: 0.572 ± 0.023	0.924PheTyr: 0.924 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	8.734GlyAla: 8.734 ± 0.09	0.771GlyCys: 0.771 ± 0.024	4.118GlyAsp: 4.118 ± 0.065	4.799GlyGlu: 4.799 ± 0.064	3.679GlyPhe: 3.679 ± 0.054	7.172GlyGly: 7.172 ± 0.112	1.932GlyHis: 1.932 ± 0.043	4.724GlyIle: 4.724 ± 0.071	3.07GlyLys: 3.07 ± 0.056	9.042GlyLeu: 9.042 ± 0.089	2.221GlyMet: 2.221 ± 0.04	2.195GlyAsn: 2.195 ± 0.064	3.451GlyPro: 3.451 ± 0.052	3.122GlyGln: 3.122 ± 0.049	6.224GlyArg: 6.224 ± 0.076	5.167GlySer: 5.167 ± 0.09	4.726GlyThr: 4.726 ± 0.07	5.893GlyVal: 5.893 ± 0.073	1.313GlyTrp: 1.313 ± 0.03	2.18GlyTyr: 2.18 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.308HisAla: 2.308 ± 0.042	0.232HisCys: 0.232 ± 0.015	1.18HisAsp: 1.18 ± 0.031	1.21HisGlu: 1.21 ± 0.027	0.816HisPhe: 0.816 ± 0.026	1.927HisGly: 1.927 ± 0.037	0.577HisHis: 0.577 ± 0.024	0.918HisIle: 0.918 ± 0.027	0.518HisLys: 0.518 ± 0.02	2.176HisLeu: 2.176 ± 0.047	0.496HisMet: 0.496 ± 0.017	0.494HisAsn: 0.494 ± 0.018	1.412HisPro: 1.412 ± 0.034	0.622HisGln: 0.622 ± 0.024	1.559HisArg: 1.559 ± 0.04	0.94HisSer: 0.94 ± 0.027	0.838HisThr: 0.838 ± 0.024	1.585HisVal: 1.585 ± 0.037	0.326HisTrp: 0.326 ± 0.017	0.496HisTyr: 0.496 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.963IleAla: 6.963 ± 0.082	0.478IleCys: 0.478 ± 0.019	3.442IleAsp: 3.442 ± 0.058	3.702IleGlu: 3.702 ± 0.052	1.612IlePhe: 1.612 ± 0.042	5.19IleGly: 5.19 ± 0.076	0.95IleHis: 0.95 ± 0.029	2.385IleIle: 2.385 ± 0.051	1.5IleLys: 1.5 ± 0.035	4.998IleLeu: 4.998 ± 0.072	1.089IleMet: 1.089 ± 0.03	1.369IleAsn: 1.369 ± 0.034	2.575IlePro: 2.575 ± 0.051	1.401IleGln: 1.401 ± 0.036	3.463IleArg: 3.463 ± 0.046	2.745IleSer: 2.745 ± 0.047	2.798IleThr: 2.798 ± 0.051	4.528IleVal: 4.528 ± 0.065	0.57IleTrp: 0.57 ± 0.024	1.121IleTyr: 1.121 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.684LysAla: 4.684 ± 0.068	0.149LysCys: 0.149 ± 0.011	2.012LysAsp: 2.012 ± 0.039	1.839LysGlu: 1.839 ± 0.039	0.848LysPhe: 0.848 ± 0.027	2.905LysGly: 2.905 ± 0.049	0.65LysHis: 0.65 ± 0.02	1.714LysIle: 1.714 ± 0.041	1.284LysLys: 1.284 ± 0.042	3.273LysLeu: 3.273 ± 0.051	0.757LysMet: 0.757 ± 0.028	0.954LysAsn: 0.954 ± 0.034	2.258LysPro: 2.258 ± 0.044	1.066LysGln: 1.066 ± 0.027	2.516LysArg: 2.516 ± 0.045	1.774LysSer: 1.774 ± 0.041	1.913LysThr: 1.913 ± 0.04	2.639LysVal: 2.639 ± 0.052	0.306LysTrp: 0.306 ± 0.015	0.571LysTyr: 0.571 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	12.944LeuAla: 12.944 ± 0.12	0.812LeuCys: 0.812 ± 0.027	6.002LeuAsp: 6.002 ± 0.073	5.594LeuGlu: 5.594 ± 0.062	3.609LeuPhe: 3.609 ± 0.056	8.277LeuGly: 8.277 ± 0.088	1.924LeuHis: 1.924 ± 0.042	5.265LeuIle: 5.265 ± 0.074	3.981LeuLys: 3.981 ± 0.062	9.426LeuLeu: 9.426 ± 0.123	2.349LeuMet: 2.349 ± 0.047	2.777LeuAsn: 2.777 ± 0.04	5.472LeuPro: 5.472 ± 0.069	3.093LeuGln: 3.093 ± 0.048	6.971LeuArg: 6.971 ± 0.079	6.703LeuSer: 6.703 ± 0.084	5.629LeuThr: 5.629 ± 0.073	7.895LeuVal: 7.895 ± 0.084	1.199LeuTrp: 1.199 ± 0.03	2.04LeuTyr: 2.04 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	3.116MetAla: 3.116 ± 0.05	0.124MetCys: 0.124 ± 0.008	1.108MetAsp: 1.108 ± 0.028	1.159MetGlu: 1.159 ± 0.032	0.668MetPhe: 0.668 ± 0.026	1.808MetGly: 1.808 ± 0.036	0.436MetHis: 0.436 ± 0.017	1.346MetIle: 1.346 ± 0.032	1.011MetLys: 1.011 ± 0.027	2.367MetLeu: 2.367 ± 0.045	0.647MetMet: 0.647 ± 0.025	0.833MetAsn: 0.833 ± 0.026	1.551MetPro: 1.551 ± 0.034	0.822MetGln: 0.822 ± 0.025	1.833MetArg: 1.833 ± 0.039	1.636MetSer: 1.636 ± 0.041	1.835MetThr: 1.835 ± 0.043	1.66MetVal: 1.66 ± 0.032	0.192MetTrp: 0.192 ± 0.01	0.296MetTyr: 0.296 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.036AsnAla: 3.036 ± 0.053	0.194AsnCys: 0.194 ± 0.012	1.531AsnAsp: 1.531 ± 0.045	1.371AsnGlu: 1.371 ± 0.038	0.917AsnPhe: 0.917 ± 0.026	2.416AsnGly: 2.416 ± 0.049	0.517AsnHis: 0.517 ± 0.021	1.316AsnIle: 1.316 ± 0.036	0.779AsnLys: 0.779 ± 0.027	2.807AsnLeu: 2.807 ± 0.047	0.597AsnMet: 0.597 ± 0.025	0.719AsnAsn: 0.719 ± 0.028	1.943AsnPro: 1.943 ± 0.037	0.8AsnGln: 0.8 ± 0.027	1.82AsnArg: 1.82 ± 0.037	1.184AsnSer: 1.184 ± 0.029	1.289AsnThr: 1.289 ± 0.039	2.037AsnVal: 2.037 ± 0.048	0.375AsnTrp: 0.375 ± 0.018	0.628AsnTyr: 0.628 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	5.802ProAla: 5.802 ± 0.068	0.304ProCys: 0.304 ± 0.014	3.473ProAsp: 3.473 ± 0.056	3.873ProGlu: 3.873 ± 0.053	2.039ProPhe: 2.039 ± 0.042	4.458ProGly: 4.458 ± 0.065	1.204ProHis: 1.204 ± 0.03	2.384ProIle: 2.384 ± 0.042	1.871ProLys: 1.871 ± 0.039	4.719ProLeu: 4.719 ± 0.063	1.196ProMet: 1.196 ± 0.031	1.506ProAsn: 1.506 ± 0.038	2.397ProPro: 2.397 ± 0.047	1.808ProGln: 1.808 ± 0.039	2.998ProArg: 2.998 ± 0.053	3.122ProSer: 3.122 ± 0.053	2.549ProThr: 2.549 ± 0.047	4.387ProVal: 4.387 ± 0.058	0.677ProTrp: 0.677 ± 0.025	1.184ProTyr: 1.184 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	4.606GlnAla: 4.606 ± 0.073	0.181GlnCys: 0.181 ± 0.012	1.809GlnAsp: 1.809 ± 0.037	1.922GlnGlu: 1.922 ± 0.045	1.006GlnPhe: 1.006 ± 0.029	2.791GlnGly: 2.791 ± 0.05	0.695GlnHis: 0.695 ± 0.021	1.807GlnIle: 1.807 ± 0.042	1.138GlnLys: 1.138 ± 0.029	2.774GlnLeu: 2.774 ± 0.048	0.865GlnMet: 0.865 ± 0.027	0.928GlnAsn: 0.928 ± 0.025	1.889GlnPro: 1.889 ± 0.041	1.311GlnGln: 1.311 ± 0.041	2.598GlnArg: 2.598 ± 0.05	1.79GlnSer: 1.79 ± 0.035	1.764GlnThr: 1.764 ± 0.042	2.619GlnVal: 2.619 ± 0.045	0.401GlnTrp: 0.401 ± 0.018	0.59GlnTyr: 0.59 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	7.378ArgAla: 7.378 ± 0.084	0.486ArgCys: 0.486 ± 0.019	3.911ArgAsp: 3.911 ± 0.061	4.65ArgGlu: 4.65 ± 0.055	2.996ArgPhe: 2.996 ± 0.055	4.869ArgGly: 4.869 ± 0.071	1.693ArgHis: 1.693 ± 0.034	4.262ArgIle: 4.262 ± 0.062	2.308ArgLys: 2.308 ± 0.049	8.137ArgLeu: 8.137 ± 0.089	1.889ArgMet: 1.889 ± 0.043	1.957ArgAsn: 1.957 ± 0.039	3.564ArgPro: 3.564 ± 0.064	2.784ArgGln: 2.784 ± 0.045	5.912ArgArg: 5.912 ± 0.086	4.112ArgSer: 4.112 ± 0.071	3.58ArgThr: 3.58 ± 0.052	5.046ArgVal: 5.046 ± 0.071	1.007ArgTrp: 1.007 ± 0.028	1.731ArgTyr: 1.731 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	5.933SerAla: 5.933 ± 0.073	0.389SerCys: 0.389 ± 0.017	2.939SerAsp: 2.939 ± 0.046	2.966SerGlu: 2.966 ± 0.055	2.399SerPhe: 2.399 ± 0.041	5.494SerGly: 5.494 ± 0.078	1.169SerHis: 1.169 ± 0.028	2.913SerIle: 2.913 ± 0.048	1.684SerLys: 1.684 ± 0.04	5.964SerLeu: 5.964 ± 0.073	1.343SerMet: 1.343 ± 0.032	1.405SerAsn: 1.405 ± 0.035	2.915SerPro: 2.915 ± 0.047	1.834SerGln: 1.834 ± 0.039	3.976SerArg: 3.976 ± 0.057	3.161SerSer: 3.161 ± 0.058	2.838SerThr: 2.838 ± 0.05	3.958SerVal: 3.958 ± 0.054	0.744SerTrp: 0.744 ± 0.027	1.345SerTyr: 1.345 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	5.751ThrAla: 5.751 ± 0.077	0.412ThrCys: 0.412 ± 0.017	2.707ThrAsp: 2.707 ± 0.049	2.711ThrGlu: 2.711 ± 0.049	2.072ThrPhe: 2.072 ± 0.041	5.059ThrGly: 5.059 ± 0.076	1.012ThrHis: 1.012 ± 0.028	3.128ThrIle: 3.128 ± 0.058	1.699ThrLys: 1.699 ± 0.039	5.978ThrLeu: 5.978 ± 0.063	1.22ThrMet: 1.22 ± 0.033	1.292ThrAsn: 1.292 ± 0.034	3.125ThrPro: 3.125 ± 0.053	1.485ThrGln: 1.485 ± 0.039	3.412ThrArg: 3.412 ± 0.05	2.948ThrSer: 2.948 ± 0.053	2.845ThrThr: 2.845 ± 0.057	4.326ThrVal: 4.326 ± 0.069	0.719ThrTrp: 0.719 ± 0.027	1.228ThrTyr: 1.228 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	8.846ValAla: 8.846 ± 0.097	0.596ValCys: 0.596 ± 0.023	4.019ValAsp: 4.019 ± 0.066	5.024ValGlu: 5.024 ± 0.066	2.765ValPhe: 2.765 ± 0.046	5.643ValGly: 5.643 ± 0.074	1.431ValHis: 1.431 ± 0.034	4.098ValIle: 4.098 ± 0.06	2.513ValLys: 2.513 ± 0.052	7.79ValLeu: 7.79 ± 0.091	1.884ValMet: 1.884 ± 0.035	2.003ValAsn: 2.003 ± 0.045	3.897ValPro: 3.897 ± 0.062	2.36ValGln: 2.36 ± 0.044	5.129ValArg: 5.129 ± 0.062	4.437ValSer: 4.437 ± 0.054	4.626ValThr: 4.626 ± 0.076	6.117ValVal: 6.117 ± 0.071	0.91ValTrp: 0.91 ± 0.028	1.536ValTyr: 1.536 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.213TrpAla: 1.213 ± 0.034	0.122TrpCys: 0.122 ± 0.009	0.645TrpAsp: 0.645 ± 0.024	0.576TrpGlu: 0.576 ± 0.022	0.505TrpPhe: 0.505 ± 0.021	0.894TrpGly: 0.894 ± 0.028	0.314TrpHis: 0.314 ± 0.015	0.698TrpIle: 0.698 ± 0.023	0.43TrpLys: 0.43 ± 0.02	1.577TrpLeu: 1.577 ± 0.041	0.343TrpMet: 0.343 ± 0.019	0.433TrpAsn: 0.433 ± 0.019	0.675TrpPro: 0.675 ± 0.023	0.556TrpGln: 0.556 ± 0.023	1.13TrpArg: 1.13 ± 0.027	0.858TrpSer: 0.858 ± 0.03	0.792TrpThr: 0.792 ± 0.026	0.783TrpVal: 0.783 ± 0.024	0.214TrpTrp: 0.214 ± 0.014	0.294TrpTyr: 0.294 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.435TyrAla: 2.435 ± 0.041	0.222TyrCys: 0.222 ± 0.014	1.416TyrAsp: 1.416 ± 0.031	1.319TyrGlu: 1.319 ± 0.032	0.861TyrPhe: 0.861 ± 0.027	2.141TyrGly: 2.141 ± 0.045	0.462TyrHis: 0.462 ± 0.02	0.882TyrIle: 0.882 ± 0.025	0.662TyrLys: 0.662 ± 0.021	2.158TyrLeu: 2.158 ± 0.044	0.373TyrMet: 0.373 ± 0.016	0.61TyrAsn: 0.61 ± 0.023	1.128TyrPro: 1.128 ± 0.032	0.674TyrGln: 0.674 ± 0.023	1.826TyrArg: 1.826 ± 0.044	1.089TyrSer: 1.089 ± 0.029	1.099TyrThr: 1.099 ± 0.029	1.639TyrVal: 1.639 ± 0.04	0.356TyrTrp: 0.356 ± 0.017	0.593TyrTyr: 0.593 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4595 proteins (1331630 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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