Habropoda laboriosa
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12781 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0L7RCG6|A0A0L7RCG6_9HYME Transmembrane protease serine 6 OS=Habropoda laboriosa OX=597456 GN=WH47_10671 PE=3 SV=1
MM1 pKa = 7.43 SCDD4 pKa = 5.86 LLPEE8 pKa = 4.07 WLNLFLDD15 pKa = 4.29 DD16 pKa = 6.24 VEE18 pKa = 4.78 TTLCLPILLIMVHH31 pKa = 5.84 CTVQFIINHH40 pKa = 5.75 AMDD43 pKa = 3.92 IGLTFYY49 pKa = 9.97 QTVIQKK55 pKa = 10.11 SDD57 pKa = 3.34 EE58 pKa = 4.22 EE59 pKa = 3.98 EE60 pKa = 4.93 AEE62 pKa = 4.33 EE63 pKa = 5.03 VEE65 pKa = 5.28 CDD67 pKa = 3.8 NAKK70 pKa = 10.47 CGQDD74 pKa = 3.56 LLSKK78 pKa = 10.64 IKK80 pKa = 10.77 GFFYY84 pKa = 10.82 NLSLGFNTDD93 pKa = 2.83 SKK95 pKa = 10.64 PEE97 pKa = 3.84 EE98 pKa = 4.08 RR99 pKa = 11.84 APPSLVRR106 pKa = 11.84 PALQEE111 pKa = 4.05 EE112 pKa = 4.28 DD113 pKa = 2.8 WDD115 pKa = 3.99 YY116 pKa = 11.92 CEE118 pKa = 6.01 EE119 pKa = 4.7 DD120 pKa = 4.59 DD121 pKa = 5.72 EE122 pKa = 5.25 EE123 pKa = 4.38 NLL125 pKa = 4.32
Molecular weight: 14.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.884
IPC_protein 3.834
Toseland 3.643
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.668
Grimsley 3.554
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.088
Thurlkill 3.681
EMBOSS 3.719
Sillero 3.948
Patrickios 0.985
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.854
Protein with the highest isoelectric point:
>tr|A0A0L7QRY3|A0A0L7QRY3_9HYME Uncharacterized protein OS=Habropoda laboriosa OX=597456 GN=WH47_06090 PE=4 SV=1
PP1 pKa = 7.09 NSKK4 pKa = 10.45 SPTSRR9 pKa = 11.84 GPKK12 pKa = 9.56 SKK14 pKa = 10.84 SPTSKK19 pKa = 10.54 GPKK22 pKa = 9.51 SKK24 pKa = 10.51 IPTSKK29 pKa = 10.54 GPKK32 pKa = 9.44 SKK34 pKa = 10.52 IPTFKK39 pKa = 10.68 GPKK42 pKa = 9.18 SKK44 pKa = 11.0 SPTSKK49 pKa = 10.54 GPKK52 pKa = 9.12 SKK54 pKa = 10.87 SPTSRR59 pKa = 11.84 GPKK62 pKa = 9.56 SKK64 pKa = 10.84 SPTSKK69 pKa = 10.57 GPKK72 pKa = 9.39 SKK74 pKa = 10.4 ISTFKK79 pKa = 10.76 GPKK82 pKa = 9.38 SKK84 pKa = 11.01 SPTSKK89 pKa = 10.71 SPTSKK94 pKa = 10.56 GPKK97 pKa = 9.14 SKK99 pKa = 10.98 SPTFKK104 pKa = 10.69 GPKK107 pKa = 9.22 SKK109 pKa = 11.0 SPTSKK114 pKa = 10.54 GPKK117 pKa = 9.22 SKK119 pKa = 10.95 SPTSKK124 pKa = 10.54 GPKK127 pKa = 9.04 SKK129 pKa = 10.67 SPKK132 pKa = 9.55 FKK134 pKa = 10.34 VSKK137 pKa = 10.49 FKK139 pKa = 10.69 ILNSKK144 pKa = 10.47 SPTFKK149 pKa = 10.65 SPKK152 pKa = 9.47 SKK154 pKa = 10.93 SPTSKK159 pKa = 10.54 GPKK162 pKa = 9.22 SKK164 pKa = 10.95 SPTSKK169 pKa = 10.54 GPKK172 pKa = 9.17 SKK174 pKa = 10.59 SPKK177 pKa = 9.79 SKK179 pKa = 10.43 GPKK182 pKa = 9.16 SKK184 pKa = 10.62 SPKK187 pKa = 9.19 FKK189 pKa = 10.74 SPRR192 pKa = 11.84 AQSQRR197 pKa = 11.84 VQSPRR202 pKa = 11.84 AKK204 pKa = 10.41 NN205 pKa = 3.16
Molecular weight: 21.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.366
IPC2_protein 11.242
IPC_protein 12.457
Toseland 12.764
ProMoST 13.115
Dawson 12.778
Bjellqvist 12.676
Wikipedia 13.159
Rodwell 13.29
Grimsley 12.808
Solomon 13.173
Lehninger 13.086
Nozaki 12.749
DTASelect 12.676
Thurlkill 12.749
EMBOSS 13.232
Sillero 12.749
Patrickios 12.998
IPC_peptide 13.173
IPC2_peptide 12.106
IPC2.peptide.svr19 8.801
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12781
0
12781
5864692
25
18728
458.9
51.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.904 ± 0.024
2.045 ± 0.041
5.253 ± 0.018
6.994 ± 0.042
3.594 ± 0.018
5.456 ± 0.033
2.487 ± 0.013
5.728 ± 0.021
6.557 ± 0.035
8.987 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.205 ± 0.011
5.162 ± 0.022
5.024 ± 0.033
4.254 ± 0.022
5.661 ± 0.031
8.129 ± 0.031
6.077 ± 0.033
6.131 ± 0.019
1.088 ± 0.008
3.156 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here