Bordetella trematum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Bordetella

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A157S7Z4|A0A157S7Z4_9BORD Outer-membrane lipoprotein carrier protein OS=Bordetella trematum OX=123899 GN=lolA PE=3 SV=1
MM1 pKa = 7.41TPNTSNPALADD12 pKa = 3.59AALTATNLWGQVYY25 pKa = 10.0LGCRR29 pKa = 11.84WLYY32 pKa = 9.9GIPHH36 pKa = 5.82QAADD40 pKa = 3.65AVADD44 pKa = 3.77EE45 pKa = 4.99AEE47 pKa = 4.31EE48 pKa = 4.27SFEE51 pKa = 5.47GDD53 pKa = 3.37LCTGLDD59 pKa = 3.8DD60 pKa = 4.68LHH62 pKa = 5.86WTINEE67 pKa = 3.83IARR70 pKa = 11.84ADD72 pKa = 3.36

Molecular weight: 7.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A157SDK6|A0A157SDK6_9BORD LysR family transcriptional regulator OS=Bordetella trematum OX=123899 GN=benM_4 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.26RR14 pKa = 11.84THH16 pKa = 5.86GFRR19 pKa = 11.84LRR21 pKa = 11.84MKK23 pKa = 9.06TRR25 pKa = 11.84AGRR28 pKa = 11.84AILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.68GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4108

0

4108

1346400

29

12096

327.8

35.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.155 ± 0.059

0.928 ± 0.016

5.219 ± 0.031

5.066 ± 0.041

3.298 ± 0.027

8.513 ± 0.052

2.134 ± 0.021

4.381 ± 0.027

2.614 ± 0.033

11.236 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.021

2.471 ± 0.026

5.39 ± 0.038

4.442 ± 0.029

7.36 ± 0.048

5.288 ± 0.034

4.862 ± 0.037

7.325 ± 0.037

1.454 ± 0.019

2.381 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski