Jeongeupia sp. USM3
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3426 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D9BDF9|A0A1D9BDF9_9NEIS MFS domain-containing protein OS=Jeongeupia sp. USM3 OX=1906741 GN=BJP62_07780 PE=4 SV=1
MM1 pKa = 6.53 TTATTEE7 pKa = 4.04 MPSPFLFTDD16 pKa = 3.93 AAANKK21 pKa = 8.84 VAEE24 pKa = 4.96 LIAEE28 pKa = 4.35 EE29 pKa = 4.62 GNPDD33 pKa = 2.99 LKK35 pKa = 11.14 LRR37 pKa = 11.84 VFVTGGGCSGFQYY50 pKa = 10.94 GFTFDD55 pKa = 5.2 EE56 pKa = 4.78 IVNDD60 pKa = 5.42 DD61 pKa = 3.59 DD62 pKa = 4.79 TEE64 pKa = 4.33 VNKK67 pKa = 10.98 AGVTLLVDD75 pKa = 3.74 PMSYY79 pKa = 10.34 QYY81 pKa = 11.57 LVGAEE86 pKa = 3.75 IDD88 pKa = 3.99 YY89 pKa = 11.25 VDD91 pKa = 4.35 SLEE94 pKa = 4.56 GSQFTIKK101 pKa = 10.63 NPNAQSTCGCGSSFSVV117 pKa = 3.54
Molecular weight: 12.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.795
IPC_protein 3.732
Toseland 3.528
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.439
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.846
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|A0A1D9BES9|A0A1D9BES9_9NEIS Uncharacterized protein OS=Jeongeupia sp. USM3 OX=1906741 GN=BJP62_11220 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVIRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.58 RR14 pKa = 11.84 THH16 pKa = 6.08 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 9.68 TNGGRR28 pKa = 11.84 NVIASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.13 GRR39 pKa = 11.84 AVLSAA44 pKa = 3.96
Molecular weight: 5.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.618
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.34
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3426
0
3426
1090397
29
1911
318.3
34.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.721 ± 0.056
0.887 ± 0.012
5.744 ± 0.03
5.098 ± 0.039
3.567 ± 0.028
8.391 ± 0.036
2.157 ± 0.021
4.651 ± 0.031
3.424 ± 0.039
11.185 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.212 ± 0.019
2.739 ± 0.023
4.985 ± 0.028
3.886 ± 0.03
6.755 ± 0.044
5.047 ± 0.031
4.997 ± 0.03
7.526 ± 0.04
1.498 ± 0.02
2.531 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here