Hubei narna-like virus 11

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KIN3|A0A1L3KIN3_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 11 OX=1922941 PE=4 SV=1
MM1 pKa = 7.79ISPFHH6 pKa = 6.66ASDD9 pKa = 3.42VKK11 pKa = 11.21SNSFTSLLTYY21 pKa = 10.79VDD23 pKa = 4.94LLCDD27 pKa = 4.62FNPKK31 pKa = 9.68GRR33 pKa = 11.84LRR35 pKa = 11.84VPLVPLLGRR44 pKa = 11.84ISLSDD49 pKa = 4.2LYY51 pKa = 10.89TKK53 pKa = 10.08EE54 pKa = 3.93EE55 pKa = 4.33WNQSDD60 pKa = 3.82TFYY63 pKa = 11.21LIGPHH68 pKa = 6.45GSDD71 pKa = 5.01SITTNPTLLSALNLRR86 pKa = 11.84TLFPADD92 pKa = 3.99CGPVEE97 pKa = 4.05HH98 pKa = 7.09SLFFGPHH105 pKa = 5.48SPFPPGTTFQYY116 pKa = 10.61IPGGSSYY123 pKa = 12.06SMLFFLRR130 pKa = 11.84SILEE134 pKa = 3.96VYY136 pKa = 10.42IDD138 pKa = 4.89FFTSDD143 pKa = 3.82LVTPSMRR150 pKa = 11.84DD151 pKa = 2.7RR152 pKa = 11.84FLAYY156 pKa = 10.19QPTSQSACIAYY167 pKa = 10.11LSSLGSSFVKK177 pKa = 10.22VVKK180 pKa = 10.38FYY182 pKa = 10.13TAYY185 pKa = 10.12PMAAFLRR192 pKa = 11.84QEE194 pKa = 4.39DD195 pKa = 4.18MPPRR199 pKa = 11.84PSQIPEE205 pKa = 3.55VRR207 pKa = 11.84TWLWDD212 pKa = 3.1GDD214 pKa = 3.54FRR216 pKa = 11.84KK217 pKa = 9.54FLKK220 pKa = 10.67QITLYY225 pKa = 10.61RR226 pKa = 11.84NPEE229 pKa = 4.03YY230 pKa = 10.95APLIWGTLQGVKK242 pKa = 9.96RR243 pKa = 11.84GCASVPDD250 pKa = 4.11TFVSDD255 pKa = 3.98SYY257 pKa = 11.46ASHH260 pKa = 6.8FRR262 pKa = 11.84TMAMEE267 pKa = 4.26SRR269 pKa = 11.84NSLLQEE275 pKa = 3.96KK276 pKa = 10.39ALDD279 pKa = 3.75LKK281 pKa = 10.96EE282 pKa = 4.47ILLNPDD288 pKa = 2.69NVLPLWGDD296 pKa = 3.43EE297 pKa = 4.07NEE299 pKa = 4.35RR300 pKa = 11.84ASEE303 pKa = 4.1LEE305 pKa = 3.93EE306 pKa = 4.24EE307 pKa = 4.49IKK309 pKa = 10.68ILEE312 pKa = 4.69DD313 pKa = 2.9KK314 pKa = 10.77TGIRR318 pKa = 11.84NTRR321 pKa = 11.84AKK323 pKa = 9.53ATLVFNILPPGSDD336 pKa = 3.75FNGTIYY342 pKa = 10.63RR343 pKa = 11.84PIKK346 pKa = 9.97MEE348 pKa = 4.16EE349 pKa = 3.71FDD351 pKa = 5.32ALDD354 pKa = 3.78SPIEE358 pKa = 4.17PSSSASYY365 pKa = 9.98RR366 pKa = 11.84YY367 pKa = 9.74SSRR370 pKa = 11.84KK371 pKa = 8.71GGQRR375 pKa = 11.84MDD377 pKa = 3.19VMQEE381 pKa = 3.53VMPPVSLPVSFPTPPSGDD399 pKa = 3.62LEE401 pKa = 5.1LNLLDD406 pKa = 5.59AIDD409 pKa = 3.9VGLVNSFSEE418 pKa = 4.3LEE420 pKa = 4.02SYY422 pKa = 10.56YY423 pKa = 10.35RR424 pKa = 11.84QQLSMGDD431 pKa = 3.37VLRR434 pKa = 11.84SVRR437 pKa = 11.84QSHH440 pKa = 7.54PDD442 pKa = 3.01HH443 pKa = 6.78QYY445 pKa = 10.2IRR447 pKa = 11.84DD448 pKa = 3.57WYY450 pKa = 10.67SGVTRR455 pKa = 11.84SWSTDD460 pKa = 3.02DD461 pKa = 4.48LRR463 pKa = 11.84YY464 pKa = 10.08SLSSILRR471 pKa = 11.84VFCFRR476 pKa = 11.84LPLTYY481 pKa = 10.64SVDD484 pKa = 3.73DD485 pKa = 4.01FLFASEE491 pKa = 4.63PPFVIPRR498 pKa = 11.84LLSQLPDD505 pKa = 5.22GIPQLISSFLGYY517 pKa = 8.76TVTPEE522 pKa = 4.11EE523 pKa = 4.13EE524 pKa = 4.16SHH526 pKa = 5.05VVKK529 pKa = 10.95VSSAYY534 pKa = 9.64TEE536 pKa = 3.57MKK538 pKa = 9.3MRR540 pKa = 11.84SEE542 pKa = 4.09NQEE545 pKa = 3.29ISSILSSITNGVTYY559 pKa = 10.29DD560 pKa = 3.88PSFGDD565 pKa = 3.73IDD567 pKa = 3.84VGLQKK572 pKa = 10.34MFVTDD577 pKa = 4.07PNSVHH582 pKa = 7.22EE583 pKa = 4.56IRR585 pKa = 11.84GHH587 pKa = 5.94LPAFFPYY594 pKa = 9.7TSPRR598 pKa = 11.84RR599 pKa = 11.84YY600 pKa = 9.99LLSLQQLQPDD610 pKa = 3.9IPVEE614 pKa = 4.05ANVIALIEE622 pKa = 4.0PLKK625 pKa = 10.84VRR627 pKa = 11.84VITTGEE633 pKa = 3.44AAAYY637 pKa = 8.97YY638 pKa = 10.04LSKK641 pKa = 10.48PLQRR645 pKa = 11.84SFWKK649 pKa = 10.25HH650 pKa = 6.07LYY652 pKa = 9.75QFPQFVLTGQPLTVEE667 pKa = 4.3ILDD670 pKa = 3.72QLEE673 pKa = 4.46DD674 pKa = 3.51STLAFCAKK682 pKa = 10.28YY683 pKa = 10.36SVPTLDD689 pKa = 2.95QWVSGDD695 pKa = 3.68YY696 pKa = 10.65KK697 pKa = 11.11GATDD701 pKa = 3.73TLDD704 pKa = 3.6ILSSMASFSAGINVLSQHH722 pKa = 5.76SQLSEE727 pKa = 3.95QFEE730 pKa = 4.36KK731 pKa = 11.18LLDD734 pKa = 3.98LNRR737 pKa = 11.84QVLDD741 pKa = 3.46RR742 pKa = 11.84HH743 pKa = 5.3TLNYY747 pKa = 9.8PKK749 pKa = 10.04GTLSSLTHH757 pKa = 5.85DD758 pKa = 4.03QIVEE762 pKa = 3.78LGGYY766 pKa = 8.9YY767 pKa = 10.7GEE769 pKa = 4.33NGEE772 pKa = 4.37VKK774 pKa = 10.46LLQGTGQLMGSPLSFPILCLINFIAYY800 pKa = 5.19WTSMEE805 pKa = 4.4KK806 pKa = 10.71YY807 pKa = 9.71LGRR810 pKa = 11.84EE811 pKa = 3.73IPDD814 pKa = 3.23FWEE817 pKa = 4.23LPVRR821 pKa = 11.84INGDD825 pKa = 3.78DD826 pKa = 3.57ILFKK830 pKa = 11.25SNTEE834 pKa = 4.36HH835 pKa = 6.19YY836 pKa = 10.11SIWKK840 pKa = 10.01EE841 pKa = 3.78EE842 pKa = 4.23VKK844 pKa = 10.69VHH846 pKa = 6.08SFEE849 pKa = 5.38LSMGKK854 pKa = 9.91NYY856 pKa = 9.88IHH858 pKa = 7.13PRR860 pKa = 11.84FLTVNSVLYY869 pKa = 10.51YY870 pKa = 7.41YY871 pKa = 9.82TRR873 pKa = 11.84HH874 pKa = 6.47HH875 pKa = 7.38DD876 pKa = 3.59GSPADD881 pKa = 4.08FGKK884 pKa = 10.67VPYY887 pKa = 10.73LNVGLLLSQSKK898 pKa = 10.37GVDD901 pKa = 3.06RR902 pKa = 11.84DD903 pKa = 3.54PQRR906 pKa = 11.84RR907 pKa = 11.84LPFAEE912 pKa = 5.15LYY914 pKa = 9.95RR915 pKa = 11.84ISVGDD920 pKa = 3.37SCNVRR925 pKa = 11.84RR926 pKa = 11.84SHH928 pKa = 7.48DD929 pKa = 3.35RR930 pKa = 11.84FIHH933 pKa = 5.55YY934 pKa = 9.73NIKK937 pKa = 10.18QIKK940 pKa = 9.15QFTNNGQFNLFIPVHH955 pKa = 5.93LGGLGFPLLPLIRR968 pKa = 11.84DD969 pKa = 3.29WRR971 pKa = 11.84VPSTDD976 pKa = 2.66GGTVFKK982 pKa = 11.35VSFTKK987 pKa = 10.37FQIQFATFLSMRR999 pKa = 11.84VEE1001 pKa = 4.33QVSLTGEE1008 pKa = 3.61IPYY1011 pKa = 10.15KK1012 pKa = 10.68YY1013 pKa = 9.79FAALVPQDD1021 pKa = 3.72LASYY1025 pKa = 9.65MRR1027 pKa = 11.84DD1028 pKa = 3.04LNTARR1033 pKa = 11.84KK1034 pKa = 9.59KK1035 pKa = 9.77IGKK1038 pKa = 9.28SEE1040 pKa = 3.98VLPPAGALSPVMYY1053 pKa = 7.95YY1054 pKa = 7.45TWKK1057 pKa = 10.48GLRR1060 pKa = 11.84PLQPLYY1066 pKa = 10.75LPSGFDD1072 pKa = 3.7LSSSTIFKK1080 pKa = 10.41DD1081 pKa = 2.99IMYY1084 pKa = 9.79VEE1086 pKa = 4.6PSSIKK1091 pKa = 9.61RR1092 pKa = 11.84TLLSYY1097 pKa = 10.78SYY1099 pKa = 9.54DD1100 pKa = 3.33TSLPLQYY1107 pKa = 10.34RR1108 pKa = 11.84LPSRR1112 pKa = 11.84SVMSEE1117 pKa = 3.77FSSFFMKK1124 pKa = 10.49GDD1126 pKa = 3.31EE1127 pKa = 4.39KK1128 pKa = 11.36GRR1130 pKa = 11.84FSLPLISSEE1139 pKa = 3.76RR1140 pKa = 11.84LLSNEE1145 pKa = 3.49QRR1147 pKa = 11.84PVYY1150 pKa = 10.2VDD1152 pKa = 4.55RR1153 pKa = 11.84EE1154 pKa = 4.21ALHH1157 pKa = 6.13QLFSTTGLSDD1167 pKa = 3.61LMGDD1171 pKa = 3.86HH1172 pKa = 6.66GPSVFLEE1179 pKa = 4.12LADD1182 pKa = 5.15LNNLPHH1188 pKa = 6.36TPIHH1192 pKa = 6.82LDD1194 pKa = 3.33YY1195 pKa = 10.99FLL1197 pKa = 6.1

Molecular weight:
135.81 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KIN3|A0A1L3KIN3_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 11 OX=1922941 PE=4 SV=1
MM1 pKa = 6.77TRR3 pKa = 11.84SRR5 pKa = 11.84ANNSRR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 7.44TRR14 pKa = 11.84RR15 pKa = 11.84PRR17 pKa = 11.84KK18 pKa = 8.45KK19 pKa = 9.86APRR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 8.77PRR26 pKa = 11.84SRR28 pKa = 11.84PRR30 pKa = 11.84TSGLSKK36 pKa = 10.91VITDD40 pKa = 3.68YY41 pKa = 11.87AKK43 pKa = 9.92MVMDD47 pKa = 5.16PCHH50 pKa = 5.73STLVRR55 pKa = 11.84TIGTATAGSVTEE67 pKa = 4.5RR68 pKa = 11.84LRR70 pKa = 11.84STIGTPTSAIVSAVGVRR87 pKa = 11.84TIVPAPSGYY96 pKa = 10.12IIWFPSYY103 pKa = 11.28HH104 pKa = 6.45NGTGNLNPANCFIYY118 pKa = 10.07TSPSSSTTTFVVNNDD133 pKa = 3.02GRR135 pKa = 11.84PTNTNALPMGTEE147 pKa = 4.37AYY149 pKa = 7.61DD150 pKa = 3.55TTGLFVQDD158 pKa = 3.96PANNLLSGAASVFSRR173 pKa = 11.84GTTVSACLQLEE184 pKa = 4.27NLVSLSTMQGQVAVVKK200 pKa = 10.31NYY202 pKa = 10.63NLAAFDD208 pKa = 4.83LNSGTNLEE216 pKa = 4.47LKK218 pKa = 10.42APTVNEE224 pKa = 3.69VFAYY228 pKa = 10.15AEE230 pKa = 3.99EE231 pKa = 4.12RR232 pKa = 11.84RR233 pKa = 11.84RR234 pKa = 11.84IDD236 pKa = 3.34PAGHH240 pKa = 4.96EE241 pKa = 4.73VVWRR245 pKa = 11.84PTDD248 pKa = 3.32ASSVARR254 pKa = 11.84GIGNEE259 pKa = 3.72ALGKK263 pKa = 10.0NFAGSPYY270 pKa = 10.35TDD272 pKa = 2.7VCFQAGQTGINSTNLAATAPEE293 pKa = 4.22HH294 pKa = 6.51QYY296 pKa = 10.95GICIAWRR303 pKa = 11.84GMAAFTTNVGLNNEE317 pKa = 4.95PITLNLIKK325 pKa = 10.4IVDD328 pKa = 4.25FEE330 pKa = 4.2LAARR334 pKa = 11.84NGQIEE339 pKa = 5.15SKK341 pKa = 9.84PKK343 pKa = 9.61PPPAPGVSKK352 pKa = 10.87DD353 pKa = 3.55VGVSFLDD360 pKa = 3.1RR361 pKa = 11.84VFPHH365 pKa = 5.97WQSTTVKK372 pKa = 9.21LAHH375 pKa = 6.79AGAVTAASMIGGSAAGNIVDD395 pKa = 5.82GIITMAQVGDD405 pKa = 3.56VTKK408 pKa = 11.27NKK410 pKa = 10.08IGGKK414 pKa = 9.3NRR416 pKa = 11.84GSQMIADD423 pKa = 4.1YY424 pKa = 11.29

Molecular weight:
45.09 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1621

424

1197

810.5

90.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.552 ± 2.544

0.802 ± 0.19

5.244 ± 0.858

4.503 ± 0.723

5.12 ± 1.033

6.416 ± 1.162

1.974 ± 0.281

5.12 ± 0.153

3.886 ± 0.062

10.611 ± 2.489

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.159 ± 0.018

4.195 ± 1.448

6.909 ± 0.391

3.64 ± 0.526

5.552 ± 0.41

10.056 ± 1.024

6.477 ± 1.605

6.539 ± 0.744

1.11 ± 0.084

4.133 ± 0.892

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski