Hubei narna-like virus 11
Average proteome isoelectric point is 7.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIN3|A0A1L3KIN3_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 11 OX=1922941 PE=4 SV=1
MM1 pKa = 7.79 ISPFHH6 pKa = 6.66 ASDD9 pKa = 3.42 VKK11 pKa = 11.21 SNSFTSLLTYY21 pKa = 10.79 VDD23 pKa = 4.94 LLCDD27 pKa = 4.62 FNPKK31 pKa = 9.68 GRR33 pKa = 11.84 LRR35 pKa = 11.84 VPLVPLLGRR44 pKa = 11.84 ISLSDD49 pKa = 4.2 LYY51 pKa = 10.89 TKK53 pKa = 10.08 EE54 pKa = 3.93 EE55 pKa = 4.33 WNQSDD60 pKa = 3.82 TFYY63 pKa = 11.21 LIGPHH68 pKa = 6.45 GSDD71 pKa = 5.01 SITTNPTLLSALNLRR86 pKa = 11.84 TLFPADD92 pKa = 3.99 CGPVEE97 pKa = 4.05 HH98 pKa = 7.09 SLFFGPHH105 pKa = 5.48 SPFPPGTTFQYY116 pKa = 10.61 IPGGSSYY123 pKa = 12.06 SMLFFLRR130 pKa = 11.84 SILEE134 pKa = 3.96 VYY136 pKa = 10.42 IDD138 pKa = 4.89 FFTSDD143 pKa = 3.82 LVTPSMRR150 pKa = 11.84 DD151 pKa = 2.7 RR152 pKa = 11.84 FLAYY156 pKa = 10.19 QPTSQSACIAYY167 pKa = 10.11 LSSLGSSFVKK177 pKa = 10.22 VVKK180 pKa = 10.38 FYY182 pKa = 10.13 TAYY185 pKa = 10.12 PMAAFLRR192 pKa = 11.84 QEE194 pKa = 4.39 DD195 pKa = 4.18 MPPRR199 pKa = 11.84 PSQIPEE205 pKa = 3.55 VRR207 pKa = 11.84 TWLWDD212 pKa = 3.1 GDD214 pKa = 3.54 FRR216 pKa = 11.84 KK217 pKa = 9.54 FLKK220 pKa = 10.67 QITLYY225 pKa = 10.61 RR226 pKa = 11.84 NPEE229 pKa = 4.03 YY230 pKa = 10.95 APLIWGTLQGVKK242 pKa = 9.96 RR243 pKa = 11.84 GCASVPDD250 pKa = 4.11 TFVSDD255 pKa = 3.98 SYY257 pKa = 11.46 ASHH260 pKa = 6.8 FRR262 pKa = 11.84 TMAMEE267 pKa = 4.26 SRR269 pKa = 11.84 NSLLQEE275 pKa = 3.96 KK276 pKa = 10.39 ALDD279 pKa = 3.75 LKK281 pKa = 10.96 EE282 pKa = 4.47 ILLNPDD288 pKa = 2.69 NVLPLWGDD296 pKa = 3.43 EE297 pKa = 4.07 NEE299 pKa = 4.35 RR300 pKa = 11.84 ASEE303 pKa = 4.1 LEE305 pKa = 3.93 EE306 pKa = 4.24 EE307 pKa = 4.49 IKK309 pKa = 10.68 ILEE312 pKa = 4.69 DD313 pKa = 2.9 KK314 pKa = 10.77 TGIRR318 pKa = 11.84 NTRR321 pKa = 11.84 AKK323 pKa = 9.53 ATLVFNILPPGSDD336 pKa = 3.75 FNGTIYY342 pKa = 10.63 RR343 pKa = 11.84 PIKK346 pKa = 9.97 MEE348 pKa = 4.16 EE349 pKa = 3.71 FDD351 pKa = 5.32 ALDD354 pKa = 3.78 SPIEE358 pKa = 4.17 PSSSASYY365 pKa = 9.98 RR366 pKa = 11.84 YY367 pKa = 9.74 SSRR370 pKa = 11.84 KK371 pKa = 8.71 GGQRR375 pKa = 11.84 MDD377 pKa = 3.19 VMQEE381 pKa = 3.53 VMPPVSLPVSFPTPPSGDD399 pKa = 3.62 LEE401 pKa = 5.1 LNLLDD406 pKa = 5.59 AIDD409 pKa = 3.9 VGLVNSFSEE418 pKa = 4.3 LEE420 pKa = 4.02 SYY422 pKa = 10.56 YY423 pKa = 10.35 RR424 pKa = 11.84 QQLSMGDD431 pKa = 3.37 VLRR434 pKa = 11.84 SVRR437 pKa = 11.84 QSHH440 pKa = 7.54 PDD442 pKa = 3.01 HH443 pKa = 6.78 QYY445 pKa = 10.2 IRR447 pKa = 11.84 DD448 pKa = 3.57 WYY450 pKa = 10.67 SGVTRR455 pKa = 11.84 SWSTDD460 pKa = 3.02 DD461 pKa = 4.48 LRR463 pKa = 11.84 YY464 pKa = 10.08 SLSSILRR471 pKa = 11.84 VFCFRR476 pKa = 11.84 LPLTYY481 pKa = 10.64 SVDD484 pKa = 3.73 DD485 pKa = 4.01 FLFASEE491 pKa = 4.63 PPFVIPRR498 pKa = 11.84 LLSQLPDD505 pKa = 5.22 GIPQLISSFLGYY517 pKa = 8.76 TVTPEE522 pKa = 4.11 EE523 pKa = 4.13 EE524 pKa = 4.16 SHH526 pKa = 5.05 VVKK529 pKa = 10.95 VSSAYY534 pKa = 9.64 TEE536 pKa = 3.57 MKK538 pKa = 9.3 MRR540 pKa = 11.84 SEE542 pKa = 4.09 NQEE545 pKa = 3.29 ISSILSSITNGVTYY559 pKa = 10.29 DD560 pKa = 3.88 PSFGDD565 pKa = 3.73 IDD567 pKa = 3.84 VGLQKK572 pKa = 10.34 MFVTDD577 pKa = 4.07 PNSVHH582 pKa = 7.22 EE583 pKa = 4.56 IRR585 pKa = 11.84 GHH587 pKa = 5.94 LPAFFPYY594 pKa = 9.7 TSPRR598 pKa = 11.84 RR599 pKa = 11.84 YY600 pKa = 9.99 LLSLQQLQPDD610 pKa = 3.9 IPVEE614 pKa = 4.05 ANVIALIEE622 pKa = 4.0 PLKK625 pKa = 10.84 VRR627 pKa = 11.84 VITTGEE633 pKa = 3.44 AAAYY637 pKa = 8.97 YY638 pKa = 10.04 LSKK641 pKa = 10.48 PLQRR645 pKa = 11.84 SFWKK649 pKa = 10.25 HH650 pKa = 6.07 LYY652 pKa = 9.75 QFPQFVLTGQPLTVEE667 pKa = 4.3 ILDD670 pKa = 3.72 QLEE673 pKa = 4.46 DD674 pKa = 3.51 STLAFCAKK682 pKa = 10.28 YY683 pKa = 10.36 SVPTLDD689 pKa = 2.95 QWVSGDD695 pKa = 3.68 YY696 pKa = 10.65 KK697 pKa = 11.11 GATDD701 pKa = 3.73 TLDD704 pKa = 3.6 ILSSMASFSAGINVLSQHH722 pKa = 5.76 SQLSEE727 pKa = 3.95 QFEE730 pKa = 4.36 KK731 pKa = 11.18 LLDD734 pKa = 3.98 LNRR737 pKa = 11.84 QVLDD741 pKa = 3.46 RR742 pKa = 11.84 HH743 pKa = 5.3 TLNYY747 pKa = 9.8 PKK749 pKa = 10.04 GTLSSLTHH757 pKa = 5.85 DD758 pKa = 4.03 QIVEE762 pKa = 3.78 LGGYY766 pKa = 8.9 YY767 pKa = 10.7 GEE769 pKa = 4.33 NGEE772 pKa = 4.37 VKK774 pKa = 10.46 LLQGTGQLMGSPLSFPILCLINFIAYY800 pKa = 5.19 WTSMEE805 pKa = 4.4 KK806 pKa = 10.71 YY807 pKa = 9.71 LGRR810 pKa = 11.84 EE811 pKa = 3.73 IPDD814 pKa = 3.23 FWEE817 pKa = 4.23 LPVRR821 pKa = 11.84 INGDD825 pKa = 3.78 DD826 pKa = 3.57 ILFKK830 pKa = 11.25 SNTEE834 pKa = 4.36 HH835 pKa = 6.19 YY836 pKa = 10.11 SIWKK840 pKa = 10.01 EE841 pKa = 3.78 EE842 pKa = 4.23 VKK844 pKa = 10.69 VHH846 pKa = 6.08 SFEE849 pKa = 5.38 LSMGKK854 pKa = 9.91 NYY856 pKa = 9.88 IHH858 pKa = 7.13 PRR860 pKa = 11.84 FLTVNSVLYY869 pKa = 10.51 YY870 pKa = 7.41 YY871 pKa = 9.82 TRR873 pKa = 11.84 HH874 pKa = 6.47 HH875 pKa = 7.38 DD876 pKa = 3.59 GSPADD881 pKa = 4.08 FGKK884 pKa = 10.67 VPYY887 pKa = 10.73 LNVGLLLSQSKK898 pKa = 10.37 GVDD901 pKa = 3.06 RR902 pKa = 11.84 DD903 pKa = 3.54 PQRR906 pKa = 11.84 RR907 pKa = 11.84 LPFAEE912 pKa = 5.15 LYY914 pKa = 9.95 RR915 pKa = 11.84 ISVGDD920 pKa = 3.37 SCNVRR925 pKa = 11.84 RR926 pKa = 11.84 SHH928 pKa = 7.48 DD929 pKa = 3.35 RR930 pKa = 11.84 FIHH933 pKa = 5.55 YY934 pKa = 9.73 NIKK937 pKa = 10.18 QIKK940 pKa = 9.15 QFTNNGQFNLFIPVHH955 pKa = 5.93 LGGLGFPLLPLIRR968 pKa = 11.84 DD969 pKa = 3.29 WRR971 pKa = 11.84 VPSTDD976 pKa = 2.66 GGTVFKK982 pKa = 11.35 VSFTKK987 pKa = 10.37 FQIQFATFLSMRR999 pKa = 11.84 VEE1001 pKa = 4.33 QVSLTGEE1008 pKa = 3.61 IPYY1011 pKa = 10.15 KK1012 pKa = 10.68 YY1013 pKa = 9.79 FAALVPQDD1021 pKa = 3.72 LASYY1025 pKa = 9.65 MRR1027 pKa = 11.84 DD1028 pKa = 3.04 LNTARR1033 pKa = 11.84 KK1034 pKa = 9.59 KK1035 pKa = 9.77 IGKK1038 pKa = 9.28 SEE1040 pKa = 3.98 VLPPAGALSPVMYY1053 pKa = 7.95 YY1054 pKa = 7.45 TWKK1057 pKa = 10.48 GLRR1060 pKa = 11.84 PLQPLYY1066 pKa = 10.75 LPSGFDD1072 pKa = 3.7 LSSSTIFKK1080 pKa = 10.41 DD1081 pKa = 2.99 IMYY1084 pKa = 9.79 VEE1086 pKa = 4.6 PSSIKK1091 pKa = 9.61 RR1092 pKa = 11.84 TLLSYY1097 pKa = 10.78 SYY1099 pKa = 9.54 DD1100 pKa = 3.33 TSLPLQYY1107 pKa = 10.34 RR1108 pKa = 11.84 LPSRR1112 pKa = 11.84 SVMSEE1117 pKa = 3.77 FSSFFMKK1124 pKa = 10.49 GDD1126 pKa = 3.31 EE1127 pKa = 4.39 KK1128 pKa = 11.36 GRR1130 pKa = 11.84 FSLPLISSEE1139 pKa = 3.76 RR1140 pKa = 11.84 LLSNEE1145 pKa = 3.49 QRR1147 pKa = 11.84 PVYY1150 pKa = 10.2 VDD1152 pKa = 4.55 RR1153 pKa = 11.84 EE1154 pKa = 4.21 ALHH1157 pKa = 6.13 QLFSTTGLSDD1167 pKa = 3.61 LMGDD1171 pKa = 3.86 HH1172 pKa = 6.66 GPSVFLEE1179 pKa = 4.12 LADD1182 pKa = 5.15 LNNLPHH1188 pKa = 6.36 TPIHH1192 pKa = 6.82 LDD1194 pKa = 3.33 YY1195 pKa = 10.99 FLL1197 pKa = 6.1
Molecular weight: 135.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.482
IPC2_protein 5.461
IPC_protein 5.499
Toseland 5.842
ProMoST 5.792
Dawson 5.69
Bjellqvist 5.728
Wikipedia 5.664
Rodwell 5.652
Grimsley 5.982
Solomon 5.69
Lehninger 5.664
Nozaki 5.919
DTASelect 6.109
Thurlkill 6.033
EMBOSS 6.008
Sillero 6.008
Patrickios 3.923
IPC_peptide 5.703
IPC2_peptide 6.008
IPC2.peptide.svr19 5.962
Protein with the highest isoelectric point:
>tr|A0A1L3KIN3|A0A1L3KIN3_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 11 OX=1922941 PE=4 SV=1
MM1 pKa = 6.77 TRR3 pKa = 11.84 SRR5 pKa = 11.84 ANNSRR10 pKa = 11.84 RR11 pKa = 11.84 KK12 pKa = 7.44 TRR14 pKa = 11.84 RR15 pKa = 11.84 PRR17 pKa = 11.84 KK18 pKa = 8.45 KK19 pKa = 9.86 APRR22 pKa = 11.84 RR23 pKa = 11.84 KK24 pKa = 8.77 PRR26 pKa = 11.84 SRR28 pKa = 11.84 PRR30 pKa = 11.84 TSGLSKK36 pKa = 10.91 VITDD40 pKa = 3.68 YY41 pKa = 11.87 AKK43 pKa = 9.92 MVMDD47 pKa = 5.16 PCHH50 pKa = 5.73 STLVRR55 pKa = 11.84 TIGTATAGSVTEE67 pKa = 4.5 RR68 pKa = 11.84 LRR70 pKa = 11.84 STIGTPTSAIVSAVGVRR87 pKa = 11.84 TIVPAPSGYY96 pKa = 10.12 IIWFPSYY103 pKa = 11.28 HH104 pKa = 6.45 NGTGNLNPANCFIYY118 pKa = 10.07 TSPSSSTTTFVVNNDD133 pKa = 3.02 GRR135 pKa = 11.84 PTNTNALPMGTEE147 pKa = 4.37 AYY149 pKa = 7.61 DD150 pKa = 3.55 TTGLFVQDD158 pKa = 3.96 PANNLLSGAASVFSRR173 pKa = 11.84 GTTVSACLQLEE184 pKa = 4.27 NLVSLSTMQGQVAVVKK200 pKa = 10.31 NYY202 pKa = 10.63 NLAAFDD208 pKa = 4.83 LNSGTNLEE216 pKa = 4.47 LKK218 pKa = 10.42 APTVNEE224 pKa = 3.69 VFAYY228 pKa = 10.15 AEE230 pKa = 3.99 EE231 pKa = 4.12 RR232 pKa = 11.84 RR233 pKa = 11.84 RR234 pKa = 11.84 IDD236 pKa = 3.34 PAGHH240 pKa = 4.96 EE241 pKa = 4.73 VVWRR245 pKa = 11.84 PTDD248 pKa = 3.32 ASSVARR254 pKa = 11.84 GIGNEE259 pKa = 3.72 ALGKK263 pKa = 10.0 NFAGSPYY270 pKa = 10.35 TDD272 pKa = 2.7 VCFQAGQTGINSTNLAATAPEE293 pKa = 4.22 HH294 pKa = 6.51 QYY296 pKa = 10.95 GICIAWRR303 pKa = 11.84 GMAAFTTNVGLNNEE317 pKa = 4.95 PITLNLIKK325 pKa = 10.4 IVDD328 pKa = 4.25 FEE330 pKa = 4.2 LAARR334 pKa = 11.84 NGQIEE339 pKa = 5.15 SKK341 pKa = 9.84 PKK343 pKa = 9.61 PPPAPGVSKK352 pKa = 10.87 DD353 pKa = 3.55 VGVSFLDD360 pKa = 3.1 RR361 pKa = 11.84 VFPHH365 pKa = 5.97 WQSTTVKK372 pKa = 9.21 LAHH375 pKa = 6.79 AGAVTAASMIGGSAAGNIVDD395 pKa = 5.82 GIITMAQVGDD405 pKa = 3.56 VTKK408 pKa = 11.27 NKK410 pKa = 10.08 IGGKK414 pKa = 9.3 NRR416 pKa = 11.84 GSQMIADD423 pKa = 4.1 YY424 pKa = 11.29
Molecular weight: 45.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.189
IPC2_protein 9.194
IPC_protein 9.224
Toseland 9.867
ProMoST 9.619
Dawson 10.101
Bjellqvist 9.809
Wikipedia 10.277
Rodwell 10.394
Grimsley 10.175
Solomon 10.131
Lehninger 10.087
Nozaki 9.926
DTASelect 9.78
Thurlkill 9.94
EMBOSS 10.277
Sillero 10.028
Patrickios 9.56
IPC_peptide 10.116
IPC2_peptide 8.595
IPC2.peptide.svr19 8.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1621
424
1197
810.5
90.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.552 ± 2.544
0.802 ± 0.19
5.244 ± 0.858
4.503 ± 0.723
5.12 ± 1.033
6.416 ± 1.162
1.974 ± 0.281
5.12 ± 0.153
3.886 ± 0.062
10.611 ± 2.489
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.159 ± 0.018
4.195 ± 1.448
6.909 ± 0.391
3.64 ± 0.526
5.552 ± 0.41
10.056 ± 1.024
6.477 ± 1.605
6.539 ± 0.744
1.11 ± 0.084
4.133 ± 0.892
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here