Sphingobium sp. SCG-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3938 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0A5U3|A0A2L0A5U3_9SPHN Cysteine hydrolase OS=Sphingobium sp. SCG-1 OX=2072936 GN=C1T17_02675 PE=4 SV=1
MM1 pKa = 7.21VNVNFVAADD10 pKa = 3.64GKK12 pKa = 8.44TWTVEE17 pKa = 3.51ADD19 pKa = 2.96EE20 pKa = 5.11GMSVMKK26 pKa = 10.32IAMKK30 pKa = 10.75NNIPGIVADD39 pKa = 4.7CGGEE43 pKa = 4.1MSCATCHH50 pKa = 6.55IYY52 pKa = 10.11IPEE55 pKa = 4.2LWLDD59 pKa = 3.66QVGCASRR66 pKa = 11.84DD67 pKa = 3.64EE68 pKa = 4.35VDD70 pKa = 3.66MLEE73 pKa = 4.22MAIDD77 pKa = 3.97PQDD80 pKa = 3.51NSRR83 pKa = 11.84LSCQVIVDD91 pKa = 4.38DD92 pKa = 4.99SLDD95 pKa = 3.65GLTVEE100 pKa = 5.44IPATQFF106 pKa = 2.91

Molecular weight: 11.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0AF69|A0A2L0AF69_9SPHN Ferredoxin OS=Sphingobium sp. SCG-1 OX=2072936 GN=C1T17_17375 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84NILRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.06SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3938

0

3938

1300806

39

6941

330.3

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.604 ± 0.049

0.806 ± 0.013

5.954 ± 0.04

5.236 ± 0.046

3.601 ± 0.024

8.793 ± 0.083

2.006 ± 0.022

5.331 ± 0.025

3.188 ± 0.034

9.751 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.025

2.833 ± 0.033

5.143 ± 0.035

3.247 ± 0.021

7.006 ± 0.054

5.733 ± 0.029

5.459 ± 0.045

7.125 ± 0.029

1.38 ± 0.019

2.321 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski