Saccharomycodes ludwigii
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4013 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A376B2H4|A0A376B2H4_9ASCO Related to mitochondrial ferredoxin OS=Saccharomycodes ludwigii OX=36035 GN=SCODWIG_00612 PE=4 SV=1
MM1 pKa = 7.03 IHH3 pKa = 6.94 AVLICKK9 pKa = 9.88 LMKK12 pKa = 10.19 FYY14 pKa = 11.11 TPVDD18 pKa = 3.7 VNKK21 pKa = 10.08 QQILFQQVYY30 pKa = 10.71 DD31 pKa = 4.85 LINNRR36 pKa = 11.84 ISEE39 pKa = 4.34 EE40 pKa = 3.86 QSSFLVTPPSILLKK54 pKa = 11.04 DD55 pKa = 3.8 NGASAEE61 pKa = 4.05 PDD63 pKa = 3.26 GTDD66 pKa = 3.0 IQIIYY71 pKa = 10.69 KK72 pKa = 10.32 NFATLYY78 pKa = 10.13 FVFIVDD84 pKa = 3.86 DD85 pKa = 4.03 QEE87 pKa = 5.15 SEE89 pKa = 4.33 LAILDD94 pKa = 4.88 LIQTFVEE101 pKa = 4.2 CLDD104 pKa = 3.31 MCFANVCEE112 pKa = 4.36 LDD114 pKa = 5.56 LVFGWQTLQSVLEE127 pKa = 4.08 EE128 pKa = 4.08 MVQGGMVIEE137 pKa = 4.07 TDD139 pKa = 2.85 KK140 pKa = 11.8 YY141 pKa = 10.58 EE142 pKa = 3.98 IVKK145 pKa = 10.83 AIDD148 pKa = 4.24 LLNMANGNGNMGGPAGSNANTDD170 pKa = 2.99 GSYY173 pKa = 9.56 LTGSLANAFSMFTTGGFSTWGAGSS197 pKa = 3.28
Molecular weight: 21.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.808
Nozaki 3.986
DTASelect 4.19
Thurlkill 3.732
EMBOSS 3.795
Sillero 3.999
Patrickios 0.998
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.88
Protein with the highest isoelectric point:
>tr|A0A376B7W3|A0A376B7W3_9ASCO Protoporphyrinogen oxidase OS=Saccharomycodes ludwigii OX=36035 GN=SCODWIG_02355 PE=3 SV=1
MM1 pKa = 7.39 ARR3 pKa = 11.84 YY4 pKa = 8.47 GATSTNPAKK13 pKa = 10.42 SASARR18 pKa = 11.84 GSYY21 pKa = 10.51 LRR23 pKa = 11.84 VSFKK27 pKa = 9.46 NTRR30 pKa = 11.84 EE31 pKa = 3.71 TAQAISGWEE40 pKa = 3.8 LKK42 pKa = 10.15 KK43 pKa = 10.89 AQTYY47 pKa = 10.12 LEE49 pKa = 4.12 QVLDD53 pKa = 3.83 HH54 pKa = 6.22 QRR56 pKa = 11.84 AIPFRR61 pKa = 11.84 RR62 pKa = 11.84 FNSSIGRR69 pKa = 11.84 TAQGKK74 pKa = 8.99 EE75 pKa = 3.74 FGVTKK80 pKa = 10.55 ARR82 pKa = 11.84 WPAKK86 pKa = 9.04 SVKK89 pKa = 9.44 FVQGLLQNAAANAEE103 pKa = 4.33 VCFF106 pKa = 4.35
Molecular weight: 11.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.838
IPC_protein 10.496
Toseland 10.818
ProMoST 10.452
Dawson 10.906
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 11.257
Grimsley 10.95
Solomon 11.008
Lehninger 10.979
Nozaki 10.789
DTASelect 10.57
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.833
Patrickios 11.008
IPC_peptide 11.008
IPC2_peptide 9.399
IPC2.peptide.svr19 8.531
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4013
0
4013
2302366
35
4839
573.7
65.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.706 ± 0.031
1.315 ± 0.014
6.011 ± 0.027
6.271 ± 0.041
4.436 ± 0.026
4.855 ± 0.033
1.983 ± 0.012
7.258 ± 0.03
7.978 ± 0.04
9.389 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.826 ± 0.012
8.191 ± 0.051
3.811 ± 0.022
3.571 ± 0.026
3.612 ± 0.021
8.608 ± 0.046
6.236 ± 0.029
5.328 ± 0.021
0.921 ± 0.01
3.694 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here