Arthrobacter phage Racecar
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 259 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J6TN61|A0A5J6TN61_9CAUD HNH endonuclease OS=Arthrobacter phage Racecar OX=2599831 GN=252 PE=4 SV=1
MM1 pKa = 7.56 AVNFSTDD8 pKa = 3.64 DD9 pKa = 3.5 ILDD12 pKa = 3.91 LRR14 pKa = 11.84 DD15 pKa = 3.36 LAEE18 pKa = 4.14 TARR21 pKa = 11.84 EE22 pKa = 3.72 ASAILEE28 pKa = 4.52 DD29 pKa = 4.53 DD30 pKa = 4.12 EE31 pKa = 4.79 QDD33 pKa = 3.34 EE34 pKa = 5.05 SEE36 pKa = 5.1 KK37 pKa = 11.07 EE38 pKa = 3.96 DD39 pKa = 3.45 AKK41 pKa = 10.63 EE42 pKa = 3.85 TLEE45 pKa = 4.56 ALASMLSDD53 pKa = 2.97 MGYY56 pKa = 9.89 SVEE59 pKa = 4.37 ALEE62 pKa = 4.6 EE63 pKa = 4.07 DD64 pKa = 3.73 AVADD68 pKa = 4.01 EE69 pKa = 4.76 LQSIGNNYY77 pKa = 9.22 EE78 pKa = 3.83 CTLIAEE84 pKa = 4.57 SHH86 pKa = 5.46 FQSYY90 pKa = 10.31 CEE92 pKa = 4.34 EE93 pKa = 4.11 LCEE96 pKa = 4.92 DD97 pKa = 4.53 LGYY100 pKa = 10.79 LPKK103 pKa = 10.66 DD104 pKa = 3.44 LPAFLSNNINWSGVADD120 pKa = 4.73 DD121 pKa = 6.19 LKK123 pKa = 11.26 DD124 pKa = 3.93 DD125 pKa = 4.08 YY126 pKa = 12.08 NEE128 pKa = 4.12 VEE130 pKa = 4.95 LDD132 pKa = 3.32 GEE134 pKa = 4.92 TYY136 pKa = 10.69 FIRR139 pKa = 11.84 SFF141 pKa = 3.3
Molecular weight: 15.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.787
IPC2_protein 3.554
IPC_protein 3.541
Toseland 3.338
ProMoST 3.694
Dawson 3.528
Bjellqvist 3.681
Wikipedia 3.439
Rodwell 3.363
Grimsley 3.249
Solomon 3.503
Lehninger 3.465
Nozaki 3.643
DTASelect 3.821
Thurlkill 3.389
EMBOSS 3.452
Sillero 3.656
Patrickios 0.947
IPC_peptide 3.503
IPC2_peptide 3.63
IPC2.peptide.svr19 3.651
Protein with the highest isoelectric point:
>tr|A0A5J6TN24|A0A5J6TN24_9CAUD Minor tail protein OS=Arthrobacter phage Racecar OX=2599831 GN=175 PE=4 SV=1
MM1 pKa = 6.43 TTRR4 pKa = 11.84 GTSNTNDD11 pKa = 3.3 RR12 pKa = 11.84 GSAKK16 pKa = 9.78 SRR18 pKa = 11.84 RR19 pKa = 11.84 VRR21 pKa = 11.84 KK22 pKa = 9.44 QWVLDD27 pKa = 3.4 VFGNGFIAFCSFMGCKK43 pKa = 10.0 EE44 pKa = 3.95 EE45 pKa = 4.39 LTFEE49 pKa = 4.63 TITIDD54 pKa = 3.45 RR55 pKa = 11.84 YY56 pKa = 10.26 PIPGCEE62 pKa = 3.53 GGTYY66 pKa = 10.09 KK67 pKa = 10.64 RR68 pKa = 11.84 GNIRR72 pKa = 11.84 PMCAFHH78 pKa = 6.95 NSSTGSLLGHH88 pKa = 5.92 QRR90 pKa = 11.84 RR91 pKa = 11.84 KK92 pKa = 9.63 QRR94 pKa = 11.84 ASS96 pKa = 2.99
Molecular weight: 10.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.355
IPC_protein 9.677
Toseland 10.321
ProMoST 9.955
Dawson 10.452
Bjellqvist 10.16
Wikipedia 10.613
Rodwell 10.745
Grimsley 10.496
Solomon 10.526
Lehninger 10.496
Nozaki 10.394
DTASelect 10.116
Thurlkill 10.335
EMBOSS 10.716
Sillero 10.394
Patrickios 10.526
IPC_peptide 10.526
IPC2_peptide 9.414
IPC2.peptide.svr19 8.289
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
259
0
259
52617
34
4266
203.2
22.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.43 ± 0.708
0.69 ± 0.071
6.213 ± 0.202
7.347 ± 0.477
3.605 ± 0.143
7.422 ± 0.218
1.731 ± 0.124
5.365 ± 0.171
5.895 ± 0.272
7.534 ± 0.28
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.313 ± 0.131
4.219 ± 0.137
4.297 ± 0.238
3.109 ± 0.111
5.038 ± 0.253
6.612 ± 0.291
7.195 ± 0.45
6.96 ± 0.204
1.752 ± 0.094
3.271 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here