Amino acid dipepetide frequency for Arthrobacter phage Racecar

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.503AlaAla: 9.503 ± 1.648
0.456AlaCys: 0.456 ± 0.097
4.941AlaAsp: 4.941 ± 0.405
7.279AlaGlu: 7.279 ± 0.591
2.338AlaPhe: 2.338 ± 0.243
7.07AlaGly: 7.07 ± 0.959
1.311AlaHis: 1.311 ± 0.201
5.246AlaIle: 5.246 ± 0.939
5.075AlaLys: 5.075 ± 0.365
8.648AlaLeu: 8.648 ± 0.636
2.015AlaMet: 2.015 ± 0.208
4.447AlaAsn: 4.447 ± 0.828
4.542AlaPro: 4.542 ± 0.65
4.162AlaGln: 4.162 ± 0.878
4.048AlaArg: 4.048 ± 0.363
6.329AlaSer: 6.329 ± 0.918
6.253AlaThr: 6.253 ± 1.36
6.31AlaVal: 6.31 ± 0.482
1.634AlaTrp: 1.634 ± 0.277
2.699AlaTyr: 2.699 ± 0.278
0.0AlaXaa: 0.0 ± 0.0
Cys
0.304CysAla: 0.304 ± 0.098
0.114CysCys: 0.114 ± 0.063
0.494CysAsp: 0.494 ± 0.111
0.646CysGlu: 0.646 ± 0.142
0.266CysPhe: 0.266 ± 0.071
0.722CysGly: 0.722 ± 0.176
0.133CysHis: 0.133 ± 0.058
0.361CysIle: 0.361 ± 0.086
0.418CysLys: 0.418 ± 0.096
0.38CysLeu: 0.38 ± 0.092
0.057CysMet: 0.057 ± 0.035
0.228CysAsn: 0.228 ± 0.074
0.342CysPro: 0.342 ± 0.107
0.171CysGln: 0.171 ± 0.066
0.551CysArg: 0.551 ± 0.149
0.513CysSer: 0.513 ± 0.096
0.475CysThr: 0.475 ± 0.139
0.494CysVal: 0.494 ± 0.11
0.152CysTrp: 0.152 ± 0.066
0.076CysTyr: 0.076 ± 0.037
0.0CysXaa: 0.0 ± 0.0
Asp
5.892AspAla: 5.892 ± 0.391
0.38AspCys: 0.38 ± 0.109
3.459AspAsp: 3.459 ± 0.29
3.991AspGlu: 3.991 ± 0.443
2.49AspPhe: 2.49 ± 0.255
6.177AspGly: 6.177 ± 0.425
1.197AspHis: 1.197 ± 0.201
3.687AspIle: 3.687 ± 0.28
3.193AspLys: 3.193 ± 0.34
5.398AspLeu: 5.398 ± 0.335
1.33AspMet: 1.33 ± 0.203
2.129AspAsn: 2.129 ± 0.226
2.908AspPro: 2.908 ± 0.338
1.52AspGln: 1.52 ± 0.192
3.516AspArg: 3.516 ± 0.323
3.554AspSer: 3.554 ± 0.321
4.01AspThr: 4.01 ± 0.651
3.82AspVal: 3.82 ± 0.325
1.273AspTrp: 1.273 ± 0.169
2.205AspTyr: 2.205 ± 0.195
0.0AspXaa: 0.0 ± 0.0
Glu
6.633GluAla: 6.633 ± 0.62
0.551GluCys: 0.551 ± 0.118
4.827GluAsp: 4.827 ± 0.491
7.45GluGlu: 7.45 ± 1.073
2.851GluPhe: 2.851 ± 0.286
4.58GluGly: 4.58 ± 0.385
1.121GluHis: 1.121 ± 0.163
4.352GluIle: 4.352 ± 0.335
4.561GluLys: 4.561 ± 0.509
6.215GluLeu: 6.215 ± 0.326
1.653GluMet: 1.653 ± 0.233
2.889GluAsn: 2.889 ± 0.324
3.003GluPro: 3.003 ± 0.679
2.224GluGln: 2.224 ± 0.309
3.858GluArg: 3.858 ± 0.437
3.478GluSer: 3.478 ± 0.282
4.808GluThr: 4.808 ± 0.484
4.694GluVal: 4.694 ± 0.444
1.539GluTrp: 1.539 ± 0.211
2.186GluTyr: 2.186 ± 0.304
0.0GluXaa: 0.0 ± 0.0
Phe
2.528PheAla: 2.528 ± 0.23
0.209PheCys: 0.209 ± 0.073
3.231PheAsp: 3.231 ± 0.244
2.547PheGlu: 2.547 ± 0.318
1.311PhePhe: 1.311 ± 0.167
3.041PheGly: 3.041 ± 0.248
0.874PheHis: 0.874 ± 0.152
1.692PheIle: 1.692 ± 0.204
2.148PheLys: 2.148 ± 0.252
2.224PheLeu: 2.224 ± 0.252
0.76PheMet: 0.76 ± 0.126
1.844PheAsn: 1.844 ± 0.206
1.387PhePro: 1.387 ± 0.167
1.102PheGln: 1.102 ± 0.146
1.749PheArg: 1.749 ± 0.188
2.376PheSer: 2.376 ± 0.223
2.642PheThr: 2.642 ± 0.286
2.528PheVal: 2.528 ± 0.279
0.703PheTrp: 0.703 ± 0.143
1.159PheTyr: 1.159 ± 0.157
0.0PheXaa: 0.0 ± 0.0
Gly
5.759GlyAla: 5.759 ± 0.592
0.475GlyCys: 0.475 ± 0.116
5.17GlyAsp: 5.17 ± 0.424
4.941GlyGlu: 4.941 ± 0.399
2.889GlyPhe: 2.889 ± 0.231
4.827GlyGly: 4.827 ± 0.403
1.254GlyHis: 1.254 ± 0.179
4.01GlyIle: 4.01 ± 0.337
4.542GlyLys: 4.542 ± 0.39
5.55GlyLeu: 5.55 ± 0.491
2.015GlyMet: 2.015 ± 0.27
4.01GlyAsn: 4.01 ± 0.64
2.129GlyPro: 2.129 ± 0.237
2.49GlyGln: 2.49 ± 0.179
3.896GlyArg: 3.896 ± 0.308
5.626GlySer: 5.626 ± 0.645
5.873GlyThr: 5.873 ± 0.576
5.055GlyVal: 5.055 ± 0.362
1.406GlyTrp: 1.406 ± 0.248
2.3GlyTyr: 2.3 ± 0.246
0.0GlyXaa: 0.0 ± 0.0
His
1.463HisAla: 1.463 ± 0.206
0.171HisCys: 0.171 ± 0.063
1.083HisAsp: 1.083 ± 0.164
1.292HisGlu: 1.292 ± 0.204
0.646HisPhe: 0.646 ± 0.123
1.558HisGly: 1.558 ± 0.211
0.38HisHis: 0.38 ± 0.106
0.817HisIle: 0.817 ± 0.125
1.045HisLys: 1.045 ± 0.154
0.988HisLeu: 0.988 ± 0.14
0.456HisMet: 0.456 ± 0.087
0.589HisAsn: 0.589 ± 0.132
0.836HisPro: 0.836 ± 0.149
0.513HisGln: 0.513 ± 0.083
1.007HisArg: 1.007 ± 0.186
0.988HisSer: 0.988 ± 0.17
1.026HisThr: 1.026 ± 0.163
1.216HisVal: 1.216 ± 0.161
0.399HisTrp: 0.399 ± 0.105
0.836HisTyr: 0.836 ± 0.147
0.0HisXaa: 0.0 ± 0.0
Ile
5.816IleAla: 5.816 ± 0.726
0.323IleCys: 0.323 ± 0.086
3.82IleAsp: 3.82 ± 0.249
3.801IleGlu: 3.801 ± 0.371
1.501IlePhe: 1.501 ± 0.179
3.782IleGly: 3.782 ± 0.39
0.969IleHis: 0.969 ± 0.141
2.433IleIle: 2.433 ± 0.224
3.326IleLys: 3.326 ± 0.244
3.687IleLeu: 3.687 ± 0.346
0.988IleMet: 0.988 ± 0.137
2.205IleAsn: 2.205 ± 0.198
2.148IlePro: 2.148 ± 0.17
1.596IleGln: 1.596 ± 0.174
2.908IleArg: 2.908 ± 0.212
3.478IleSer: 3.478 ± 0.285
4.903IleThr: 4.903 ± 1.174
3.744IleVal: 3.744 ± 0.301
0.608IleTrp: 0.608 ± 0.107
1.615IleTyr: 1.615 ± 0.177
0.0IleXaa: 0.0 ± 0.0
Lys
5.911LysAla: 5.911 ± 0.421
0.418LysCys: 0.418 ± 0.118
3.82LysAsp: 3.82 ± 0.329
3.934LysGlu: 3.934 ± 0.456
2.585LysPhe: 2.585 ± 0.296
3.269LysGly: 3.269 ± 0.381
1.463LysHis: 1.463 ± 0.195
3.801LysIle: 3.801 ± 0.297
3.554LysLys: 3.554 ± 0.399
4.656LysLeu: 4.656 ± 0.358
2.053LysMet: 2.053 ± 0.202
2.338LysAsn: 2.338 ± 0.256
2.623LysPro: 2.623 ± 0.253
1.577LysGln: 1.577 ± 0.191
2.794LysArg: 2.794 ± 0.334
2.908LysSer: 2.908 ± 0.276
4.086LysThr: 4.086 ± 0.369
4.257LysVal: 4.257 ± 0.31
0.817LysTrp: 0.817 ± 0.117
2.072LysTyr: 2.072 ± 0.252
0.0LysXaa: 0.0 ± 0.0
Leu
6.994LeuAla: 6.994 ± 0.661
0.551LeuCys: 0.551 ± 0.122
4.903LeuAsp: 4.903 ± 0.403
5.721LeuGlu: 5.721 ± 0.472
2.243LeuPhe: 2.243 ± 0.246
5.379LeuGly: 5.379 ± 0.524
1.33LeuHis: 1.33 ± 0.149
3.592LeuIle: 3.592 ± 0.257
4.656LeuLys: 4.656 ± 0.381
5.17LeuLeu: 5.17 ± 0.445
2.053LeuMet: 2.053 ± 0.268
3.174LeuAsn: 3.174 ± 0.331
3.668LeuPro: 3.668 ± 0.361
2.509LeuGln: 2.509 ± 0.28
4.219LeuArg: 4.219 ± 0.339
5.075LeuSer: 5.075 ± 0.347
5.93LeuThr: 5.93 ± 0.348
4.637LeuVal: 4.637 ± 0.339
1.159LeuTrp: 1.159 ± 0.144
2.376LeuTyr: 2.376 ± 0.235
0.0LeuXaa: 0.0 ± 0.0
Met
2.338MetAla: 2.338 ± 0.222
0.19MetCys: 0.19 ± 0.058
1.501MetAsp: 1.501 ± 0.201
1.787MetGlu: 1.787 ± 0.238
0.931MetPhe: 0.931 ± 0.167
1.52MetGly: 1.52 ± 0.25
0.323MetHis: 0.323 ± 0.086
1.007MetIle: 1.007 ± 0.16
1.444MetLys: 1.444 ± 0.172
1.806MetLeu: 1.806 ± 0.23
0.342MetMet: 0.342 ± 0.07
1.121MetAsn: 1.121 ± 0.197
0.988MetPro: 0.988 ± 0.142
0.399MetGln: 0.399 ± 0.077
0.988MetArg: 0.988 ± 0.163
1.863MetSer: 1.863 ± 0.255
1.996MetThr: 1.996 ± 0.221
1.463MetVal: 1.463 ± 0.195
0.494MetTrp: 0.494 ± 0.11
0.627MetTyr: 0.627 ± 0.107
0.0MetXaa: 0.0 ± 0.0
Asn
3.706AsnAla: 3.706 ± 0.306
0.19AsnCys: 0.19 ± 0.056
2.148AsnAsp: 2.148 ± 0.218
2.49AsnGlu: 2.49 ± 0.29
1.368AsnPhe: 1.368 ± 0.151
4.219AsnGly: 4.219 ± 0.498
0.874AsnHis: 0.874 ± 0.151
1.787AsnIle: 1.787 ± 0.153
2.376AsnLys: 2.376 ± 0.334
3.364AsnLeu: 3.364 ± 0.311
1.254AsnMet: 1.254 ± 0.181
1.634AsnAsn: 1.634 ± 0.19
2.395AsnPro: 2.395 ± 0.242
1.178AsnGln: 1.178 ± 0.16
2.167AsnArg: 2.167 ± 0.235
2.908AsnSer: 2.908 ± 0.338
3.231AsnThr: 3.231 ± 0.457
2.832AsnVal: 2.832 ± 0.245
0.722AsnTrp: 0.722 ± 0.119
1.349AsnTyr: 1.349 ± 0.151
0.0AsnXaa: 0.0 ± 0.0
Pro
5.017ProAla: 5.017 ± 0.587
0.247ProCys: 0.247 ± 0.073
2.832ProAsp: 2.832 ± 0.327
5.474ProGlu: 5.474 ± 1.376
1.444ProPhe: 1.444 ± 0.197
2.224ProGly: 2.224 ± 0.195
0.608ProHis: 0.608 ± 0.138
1.634ProIle: 1.634 ± 0.186
2.509ProLys: 2.509 ± 0.302
2.794ProLeu: 2.794 ± 0.26
0.836ProMet: 0.836 ± 0.117
1.825ProAsn: 1.825 ± 0.261
1.444ProPro: 1.444 ± 0.158
1.311ProGln: 1.311 ± 0.206
1.52ProArg: 1.52 ± 0.189
3.117ProSer: 3.117 ± 0.245
2.813ProThr: 2.813 ± 0.257
3.193ProVal: 3.193 ± 0.287
0.475ProTrp: 0.475 ± 0.108
1.653ProTyr: 1.653 ± 0.21
0.0ProXaa: 0.0 ± 0.0
Gln
2.851GlnAla: 2.851 ± 0.358
0.228GlnCys: 0.228 ± 0.08
1.787GlnAsp: 1.787 ± 0.287
2.395GlnGlu: 2.395 ± 0.342
0.988GlnPhe: 0.988 ± 0.132
2.471GlnGly: 2.471 ± 0.223
0.361GlnHis: 0.361 ± 0.086
1.863GlnIle: 1.863 ± 0.175
2.129GlnLys: 2.129 ± 0.23
2.224GlnLeu: 2.224 ± 0.221
0.912GlnMet: 0.912 ± 0.171
1.273GlnAsn: 1.273 ± 0.168
1.235GlnPro: 1.235 ± 0.174
1.064GlnGln: 1.064 ± 0.136
1.463GlnArg: 1.463 ± 0.15
2.49GlnSer: 2.49 ± 0.514
1.977GlnThr: 1.977 ± 0.187
1.882GlnVal: 1.882 ± 0.176
0.665GlnTrp: 0.665 ± 0.101
0.836GlnTyr: 0.836 ± 0.148
0.0GlnXaa: 0.0 ± 0.0
Arg
4.029ArgAla: 4.029 ± 0.278
0.456ArgCys: 0.456 ± 0.109
3.212ArgAsp: 3.212 ± 0.31
3.459ArgGlu: 3.459 ± 0.4
1.901ArgPhe: 1.901 ± 0.2
3.421ArgGly: 3.421 ± 0.33
1.121ArgHis: 1.121 ± 0.179
3.136ArgIle: 3.136 ± 0.233
2.965ArgLys: 2.965 ± 0.318
3.972ArgLeu: 3.972 ± 0.344
1.501ArgMet: 1.501 ± 0.211
1.901ArgAsn: 1.901 ± 0.236
1.901ArgPro: 1.901 ± 0.203
1.52ArgGln: 1.52 ± 0.236
3.06ArgArg: 3.06 ± 0.35
3.288ArgSer: 3.288 ± 0.365
3.117ArgThr: 3.117 ± 0.31
4.162ArgVal: 4.162 ± 0.294
0.779ArgTrp: 0.779 ± 0.139
1.482ArgTyr: 1.482 ± 0.202
0.0ArgXaa: 0.0 ± 0.0
Ser
6.842SerAla: 6.842 ± 1.248
0.608SerCys: 0.608 ± 0.129
3.288SerAsp: 3.288 ± 0.301
3.687SerGlu: 3.687 ± 0.313
2.395SerPhe: 2.395 ± 0.264
5.797SerGly: 5.797 ± 0.602
1.007SerHis: 1.007 ± 0.197
4.656SerIle: 4.656 ± 1.09
3.63SerLys: 3.63 ± 0.27
4.618SerLeu: 4.618 ± 0.312
1.33SerMet: 1.33 ± 0.165
2.87SerAsn: 2.87 ± 0.206
2.452SerPro: 2.452 ± 0.24
1.882SerGln: 1.882 ± 0.148
3.345SerArg: 3.345 ± 0.343
5.075SerSer: 5.075 ± 0.74
4.732SerThr: 4.732 ± 0.35
4.599SerVal: 4.599 ± 0.323
1.178SerTrp: 1.178 ± 0.183
2.129SerTyr: 2.129 ± 0.28
0.0SerXaa: 0.0 ± 0.0
Thr
9.332ThrAla: 9.332 ± 3.047
0.323ThrCys: 0.323 ± 0.086
3.953ThrAsp: 3.953 ± 0.236
4.029ThrGlu: 4.029 ± 0.435
2.775ThrPhe: 2.775 ± 0.237
6.158ThrGly: 6.158 ± 0.605
1.007ThrHis: 1.007 ± 0.144
3.63ThrIle: 3.63 ± 0.271
4.333ThrLys: 4.333 ± 0.294
5.303ThrLeu: 5.303 ± 0.445
1.178ThrMet: 1.178 ± 0.191
2.908ThrAsn: 2.908 ± 0.288
4.086ThrPro: 4.086 ± 0.462
1.615ThrGln: 1.615 ± 0.155
2.908ThrArg: 2.908 ± 0.267
4.124ThrSer: 4.124 ± 0.476
4.96ThrThr: 4.96 ± 0.482
5.55ThrVal: 5.55 ± 0.393
1.197ThrTrp: 1.197 ± 0.322
2.585ThrTyr: 2.585 ± 0.252
0.0ThrXaa: 0.0 ± 0.0
Val
5.797ValAla: 5.797 ± 0.36
0.513ValCys: 0.513 ± 0.12
4.333ValAsp: 4.333 ± 0.345
5.246ValGlu: 5.246 ± 0.391
2.319ValPhe: 2.319 ± 0.227
4.637ValGly: 4.637 ± 0.328
1.083ValHis: 1.083 ± 0.153
3.44ValIle: 3.44 ± 0.282
4.276ValLys: 4.276 ± 0.376
4.865ValLeu: 4.865 ± 0.409
1.311ValMet: 1.311 ± 0.194
2.623ValAsn: 2.623 ± 0.292
3.041ValPro: 3.041 ± 0.246
2.3ValGln: 2.3 ± 0.185
3.915ValArg: 3.915 ± 0.363
5.075ValSer: 5.075 ± 0.424
5.626ValThr: 5.626 ± 0.329
5.227ValVal: 5.227 ± 0.503
1.482ValTrp: 1.482 ± 0.257
2.49ValTyr: 2.49 ± 0.277
0.0ValXaa: 0.0 ± 0.0
Trp
1.14TrpAla: 1.14 ± 0.149
0.133TrpCys: 0.133 ± 0.049
1.197TrpAsp: 1.197 ± 0.167
1.14TrpGlu: 1.14 ± 0.174
1.026TrpPhe: 1.026 ± 0.308
1.007TrpGly: 1.007 ± 0.137
0.304TrpHis: 0.304 ± 0.075
0.912TrpIle: 0.912 ± 0.124
0.912TrpLys: 0.912 ± 0.143
1.083TrpLeu: 1.083 ± 0.142
0.57TrpMet: 0.57 ± 0.117
0.893TrpAsn: 0.893 ± 0.211
0.627TrpPro: 0.627 ± 0.11
0.608TrpGln: 0.608 ± 0.098
0.836TrpArg: 0.836 ± 0.137
1.33TrpSer: 1.33 ± 0.211
1.425TrpThr: 1.425 ± 0.21
1.463TrpVal: 1.463 ± 0.204
0.247TrpTrp: 0.247 ± 0.067
0.665TrpTyr: 0.665 ± 0.129
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.452TyrAla: 2.452 ± 0.235
0.361TyrCys: 0.361 ± 0.088
2.129TyrAsp: 2.129 ± 0.283
2.167TyrGlu: 2.167 ± 0.245
1.787TyrPhe: 1.787 ± 0.206
2.357TyrGly: 2.357 ± 0.253
0.589TyrHis: 0.589 ± 0.101
1.596TyrIle: 1.596 ± 0.165
1.863TyrLys: 1.863 ± 0.204
2.395TyrLeu: 2.395 ± 0.267
0.494TyrMet: 0.494 ± 0.08
1.292TyrAsn: 1.292 ± 0.16
1.216TyrPro: 1.216 ± 0.154
1.349TyrGln: 1.349 ± 0.155
1.634TyrArg: 1.634 ± 0.2
2.528TyrSer: 2.528 ± 0.231
2.072TyrThr: 2.072 ± 0.232
2.471TyrVal: 2.471 ± 0.299
0.589TyrTrp: 0.589 ± 0.11
1.368TyrTyr: 1.368 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 259 proteins (52617 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski