Thalassocella blandensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Thalassocella

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4758 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A509E4S0|A0A509E4S0_9RHIZ Cation efflux system protein CusA OS=Thalassocella blandensis OX=2584524 GN=cusA PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.6LLRR5 pKa = 11.84TTLGMLSASLVLVACGGGGGGGGGGGGSTPTPTPDD40 pKa = 4.1PGVSCNDD47 pKa = 3.28GTPSFVTCTDD57 pKa = 3.37GQNNGPDD64 pKa = 3.64VYY66 pKa = 9.68TLHH69 pKa = 6.18GTVDD73 pKa = 3.39ADD75 pKa = 3.52YY76 pKa = 11.08TMDD79 pKa = 4.73SSYY82 pKa = 10.68EE83 pKa = 3.66WRR85 pKa = 11.84LDD87 pKa = 3.32GVVKK91 pKa = 10.73VGSGNVEE98 pKa = 3.81VTSADD103 pKa = 3.38DD104 pKa = 3.26VAAIQDD110 pKa = 3.7AGVTLTIEE118 pKa = 4.54PGTHH122 pKa = 5.69IKK124 pKa = 10.93AFDD127 pKa = 4.22DD128 pKa = 4.3GVLLVTRR135 pKa = 11.84GSKK138 pKa = 10.49LMADD142 pKa = 4.54GTATAPITFSSLDD155 pKa = 3.62DD156 pKa = 4.79GYY158 pKa = 11.44DD159 pKa = 4.34GIGEE163 pKa = 4.07WGGVIVQGFAPQYY176 pKa = 9.18GQGDD180 pKa = 3.76TGPCYY185 pKa = 10.08GTGSICNVEE194 pKa = 4.25GEE196 pKa = 4.56GGTEE200 pKa = 3.83VAVYY204 pKa = 10.42GGDD207 pKa = 3.88IEE209 pKa = 5.57DD210 pKa = 5.01DD211 pKa = 3.38NSGVIRR217 pKa = 11.84YY218 pKa = 8.28VRR220 pKa = 11.84IAEE223 pKa = 4.41GGLIAGPNNEE233 pKa = 4.28INGLTLQGVGHH244 pKa = 6.05GTVVDD249 pKa = 4.06YY250 pKa = 10.97VHH252 pKa = 6.4VHH254 pKa = 6.05GNLDD258 pKa = 4.89DD259 pKa = 4.22GVEE262 pKa = 4.17WFGGTVNVTHH272 pKa = 7.34LVLTNNDD279 pKa = 3.85DD280 pKa = 4.09DD281 pKa = 7.11DD282 pKa = 5.38IDD284 pKa = 4.01FDD286 pKa = 4.14EE287 pKa = 5.78GYY289 pKa = 10.36KK290 pKa = 11.14GNIQYY295 pKa = 10.91AIVVKK300 pKa = 10.36DD301 pKa = 3.53QTKK304 pKa = 9.61EE305 pKa = 3.89APTGSNDD312 pKa = 3.2PRR314 pKa = 11.84GIEE317 pKa = 4.38ANSSDD322 pKa = 4.53DD323 pKa = 5.44DD324 pKa = 4.19YY325 pKa = 12.22VPDD328 pKa = 4.07TNAVLANVTVIGGAVNNAEE347 pKa = 4.32GKK349 pKa = 9.7EE350 pKa = 4.03EE351 pKa = 4.07PGMRR355 pKa = 11.84LRR357 pKa = 11.84GSLTATIYY365 pKa = 10.83NSAVKK370 pKa = 10.54GFDD373 pKa = 3.29TGCIRR378 pKa = 11.84IDD380 pKa = 4.86DD381 pKa = 4.44SDD383 pKa = 3.9TDD385 pKa = 4.15GDD387 pKa = 3.94GTADD391 pKa = 4.38DD392 pKa = 4.73FSDD395 pKa = 3.64VNLINVFGDD404 pKa = 3.96CADD407 pKa = 3.33GFYY410 pKa = 10.99DD411 pKa = 6.01KK412 pKa = 10.83RR413 pKa = 11.84AADD416 pKa = 3.61NASNAVVSSVTLDD429 pKa = 2.99SAYY432 pKa = 10.83ALTEE436 pKa = 3.95SAATLGAAPAINAIVNGSGFMFDD459 pKa = 2.94QTAYY463 pKa = 10.18VGAVAPGTAMEE474 pKa = 4.45DD475 pKa = 3.22AWWYY479 pKa = 9.63GWTIPGVLSDD489 pKa = 5.45AEE491 pKa = 4.66AGDD494 pKa = 4.28DD495 pKa = 4.17APAEE499 pKa = 4.05ADD501 pKa = 3.79FVNCNHH507 pKa = 7.12ALLEE511 pKa = 4.3CTVAGTIDD519 pKa = 3.53EE520 pKa = 5.52DD521 pKa = 4.25YY522 pKa = 10.3TFVSGYY528 pKa = 7.49TWFLDD533 pKa = 3.49GTVKK537 pKa = 10.66VGNGNIEE544 pKa = 4.17VASDD548 pKa = 3.41ADD550 pKa = 3.65VAAIQAAGVTLTVQAGTNVKK570 pKa = 10.59AFDD573 pKa = 4.94DD574 pKa = 4.04GTLLVTRR581 pKa = 11.84GSKK584 pKa = 10.49LMADD588 pKa = 4.4GTAAAPITFSSLDD601 pKa = 3.41QNFSSEE607 pKa = 4.43GEE609 pKa = 3.87WGGLIIQGFAPQYY622 pKa = 9.47GQGDD626 pKa = 3.7TGPCYY631 pKa = 10.92GEE633 pKa = 3.86GTTCNVAGEE642 pKa = 4.68GGTDD646 pKa = 3.29VAVYY650 pKa = 10.13GGNIVDD656 pKa = 4.83DD657 pKa = 3.98NSGVIRR663 pKa = 11.84YY664 pKa = 8.28VRR666 pKa = 11.84IAEE669 pKa = 4.45GGLVAGPNNEE679 pKa = 4.04INGLTLQGVGYY690 pKa = 7.82GTTIEE695 pKa = 4.15YY696 pKa = 10.15VQVHH700 pKa = 6.39GNLDD704 pKa = 4.07DD705 pKa = 4.18GVEE708 pKa = 4.17WFGGTVNAKK717 pKa = 10.38YY718 pKa = 10.37LVLTNNDD725 pKa = 3.83DD726 pKa = 4.09DD727 pKa = 7.11DD728 pKa = 5.38IDD730 pKa = 4.01FDD732 pKa = 4.14EE733 pKa = 5.78GYY735 pKa = 10.36KK736 pKa = 11.14GNIQYY741 pKa = 10.94AIVVKK746 pKa = 10.02NQNKK750 pKa = 9.41AVPTGSNDD758 pKa = 3.1PRR760 pKa = 11.84GIEE763 pKa = 4.38ANSSDD768 pKa = 4.82DD769 pKa = 5.22DD770 pKa = 4.26FVPDD774 pKa = 4.11TNAVLANITLIGGPVNNSTDD794 pKa = 3.39KK795 pKa = 11.03EE796 pKa = 4.38EE797 pKa = 4.49PGMRR801 pKa = 11.84LRR803 pKa = 11.84GSLTVAIHH811 pKa = 4.87NTALKK816 pKa = 10.74GFDD819 pKa = 3.6TGCVRR824 pKa = 11.84VDD826 pKa = 4.9DD827 pKa = 4.89SDD829 pKa = 3.81TDD831 pKa = 4.04GDD833 pKa = 3.94GTADD837 pKa = 3.72DD838 pKa = 4.76FSNVTLNNIIADD850 pKa = 4.11CAGGFYY856 pKa = 10.79AKK858 pKa = 10.86GEE860 pKa = 4.08ADD862 pKa = 3.63TVNNAIEE869 pKa = 4.32STVTIDD875 pKa = 3.62EE876 pKa = 4.55AFALTDD882 pKa = 3.46AAASLGVTTAITAMDD897 pKa = 4.53NGSMFAFDD905 pKa = 3.18QTSFVGAVAPGTAAAEE921 pKa = 4.14AWWAGWTLEE930 pKa = 4.12GTLDD934 pKa = 4.91DD935 pKa = 5.91AIEE938 pKa = 4.13NNN940 pKa = 3.7

Molecular weight: 96.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A509E2X4|A0A509E2X4_9RHIZ Beta-glucanase OS=Thalassocella blandensis OX=2584524 GN=bglA_9 PE=4 SV=1
MM1 pKa = 7.34VRR3 pKa = 11.84LPEE6 pKa = 4.06NKK8 pKa = 9.31IPAAKK13 pKa = 10.17FIIARR18 pKa = 11.84KK19 pKa = 9.02RR20 pKa = 11.84MRR22 pKa = 11.84IEE24 pKa = 4.09GFTIGAKK31 pKa = 7.38TACTMALWCYY41 pKa = 9.59FAAIPSQYY49 pKa = 9.74FSRR52 pKa = 11.84IRR54 pKa = 11.84RR55 pKa = 11.84FQMLNDD61 pKa = 3.6VKK63 pKa = 11.17

Molecular weight: 7.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4758

0

4758

1693339

29

6820

355.9

39.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.536 ± 0.041

1.033 ± 0.013

5.81 ± 0.04

6.344 ± 0.039

4.258 ± 0.023

6.919 ± 0.046

2.236 ± 0.022

6.222 ± 0.029

5.064 ± 0.047

9.695 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.284 ± 0.019

4.673 ± 0.037

3.933 ± 0.025

4.409 ± 0.03

4.571 ± 0.032

7.278 ± 0.041

5.483 ± 0.043

6.782 ± 0.036

1.3 ± 0.017

3.17 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski