Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae; Methanopyrus; Methanopyrus kandleri

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8TGZ6|Q8TGZ6_METKA tRNA/rRNA cytosine-C5-methylase OS=Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) OX=190192 GN=MK0370 PE=3 SV=1
MM1 pKa = 7.53VSVNEE6 pKa = 4.03NALPLVEE13 pKa = 5.06RR14 pKa = 11.84MIEE17 pKa = 3.96RR18 pKa = 11.84AEE20 pKa = 3.93LLNVEE25 pKa = 4.32VQEE28 pKa = 4.59LEE30 pKa = 4.0NGTTVIDD37 pKa = 4.41CGVEE41 pKa = 3.57AAGGFEE47 pKa = 4.07AGLLFSEE54 pKa = 4.79VCMGGLATVEE64 pKa = 4.03LTEE67 pKa = 4.5FEE69 pKa = 4.6HH70 pKa = 7.9DD71 pKa = 4.26GLCLPAVQVTTDD83 pKa = 3.37HH84 pKa = 6.91PAVSTLAAQKK94 pKa = 10.59AGWQVQVGDD103 pKa = 4.04YY104 pKa = 10.55FAMGSGPARR113 pKa = 11.84ALALKK118 pKa = 10.2PKK120 pKa = 8.62EE121 pKa = 4.2TYY123 pKa = 10.53EE124 pKa = 4.26EE125 pKa = 3.73IDD127 pKa = 3.77YY128 pKa = 11.47EE129 pKa = 5.04DD130 pKa = 4.72DD131 pKa = 3.69ADD133 pKa = 4.28VAILCLEE140 pKa = 4.18SSEE143 pKa = 5.83LPDD146 pKa = 3.38EE147 pKa = 5.07DD148 pKa = 3.93VAEE151 pKa = 4.56HH152 pKa = 6.34VADD155 pKa = 3.8EE156 pKa = 4.89CGVDD160 pKa = 3.87PEE162 pKa = 4.17NLYY165 pKa = 11.32LLVAPTASIVGSVQVSARR183 pKa = 11.84VVEE186 pKa = 4.15TGLYY190 pKa = 10.04KK191 pKa = 10.75LLEE194 pKa = 4.06VLEE197 pKa = 4.34YY198 pKa = 11.0DD199 pKa = 3.79VTRR202 pKa = 11.84VKK204 pKa = 10.79YY205 pKa = 9.17ATGTAPIAPVADD217 pKa = 4.38DD218 pKa = 4.93DD219 pKa = 4.6GEE221 pKa = 4.97AMGRR225 pKa = 11.84TNDD228 pKa = 3.9CILYY232 pKa = 10.24GGTVYY237 pKa = 10.79LYY239 pKa = 11.15VEE241 pKa = 4.98GDD243 pKa = 3.65DD244 pKa = 4.06EE245 pKa = 4.66LPEE248 pKa = 4.18VVEE251 pKa = 4.4EE252 pKa = 4.52LPSEE256 pKa = 4.1ASEE259 pKa = 5.43DD260 pKa = 3.61YY261 pKa = 10.72GKK263 pKa = 9.98PFMKK267 pKa = 9.89IFEE270 pKa = 4.14EE271 pKa = 4.34ADD273 pKa = 3.08YY274 pKa = 11.18DD275 pKa = 4.33FYY277 pKa = 11.55KK278 pKa = 10.22IDD280 pKa = 4.08PGVFAPARR288 pKa = 11.84VVVNDD293 pKa = 4.07LSTGKK298 pKa = 8.8TYY300 pKa = 9.51TAGEE304 pKa = 4.21INVDD308 pKa = 3.57VLKK311 pKa = 10.92EE312 pKa = 4.18SFGLL316 pKa = 3.77

Molecular weight: 34.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q8TUY4|RL31_METKA 50S ribosomal protein L31e OS=Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) OX=190192 GN=rpl31e PE=3 SV=1
MM1 pKa = 7.51ARR3 pKa = 11.84VKK5 pKa = 10.61PLGKK9 pKa = 9.72KK10 pKa = 10.03LRR12 pKa = 11.84MAKK15 pKa = 10.06AIKK18 pKa = 9.32QNRR21 pKa = 11.84RR22 pKa = 11.84VPPWVVAKK30 pKa = 10.08TGGRR34 pKa = 11.84VIDD37 pKa = 3.78NPKK40 pKa = 8.35RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.1WRR45 pKa = 11.84RR46 pKa = 11.84SKK48 pKa = 10.9LKK50 pKa = 9.79PP51 pKa = 3.26

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1687

0

1687

500958

39

2042

297.0

33.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.343 ± 0.064

1.32 ± 0.029

5.798 ± 0.048

10.0 ± 0.099

2.873 ± 0.031

8.051 ± 0.059

1.931 ± 0.022

4.827 ± 0.051

4.022 ± 0.056

10.084 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.9 ± 0.027

1.916 ± 0.027

5.463 ± 0.045

1.409 ± 0.028

8.345 ± 0.076

4.621 ± 0.05

4.594 ± 0.041

10.442 ± 0.06

1.239 ± 0.029

2.821 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski