Amino acid dipepetide frequency for Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.37AlaAla: 8.37 ± 0.186
1.242AlaCys: 1.242 ± 0.054
4.308AlaAsp: 4.308 ± 0.104
7.873AlaGlu: 7.873 ± 0.152
2.659AlaPhe: 2.659 ± 0.073
6.589AlaGly: 6.589 ± 0.143
1.633AlaHis: 1.633 ± 0.065
4.158AlaIle: 4.158 ± 0.111
2.932AlaLys: 2.932 ± 0.084
9.733AlaLeu: 9.733 ± 0.177
1.687AlaMet: 1.687 ± 0.062
1.385AlaAsn: 1.385 ± 0.054
3.102AlaPro: 3.102 ± 0.085
1.236AlaGln: 1.236 ± 0.051
6.831AlaArg: 6.831 ± 0.141
3.763AlaSer: 3.763 ± 0.095
3.533AlaThr: 3.533 ± 0.094
9.172AlaVal: 9.172 ± 0.149
0.976AlaTrp: 0.976 ± 0.046
2.25AlaTyr: 2.25 ± 0.072
0.0AlaXaa: 0.0 ± 0.0
Cys
1.204CysAla: 1.204 ± 0.051
0.202CysCys: 0.202 ± 0.023
0.775CysAsp: 0.775 ± 0.042
1.1CysGlu: 1.1 ± 0.051
0.305CysPhe: 0.305 ± 0.026
1.467CysGly: 1.467 ± 0.062
0.301CysHis: 0.301 ± 0.027
0.397CysIle: 0.397 ± 0.03
0.387CysLys: 0.387 ± 0.03
1.12CysLeu: 1.12 ± 0.051
0.218CysMet: 0.218 ± 0.021
0.252CysAsn: 0.252 ± 0.023
1.298CysPro: 1.298 ± 0.068
0.174CysGln: 0.174 ± 0.017
0.996CysArg: 0.996 ± 0.045
0.697CysSer: 0.697 ± 0.045
0.609CysThr: 0.609 ± 0.039
1.198CysVal: 1.198 ± 0.053
0.162CysTrp: 0.162 ± 0.018
0.343CysTyr: 0.343 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.891AspAla: 4.891 ± 0.108
0.527AspCys: 0.527 ± 0.032
3.178AspAsp: 3.178 ± 0.088
5.462AspGlu: 5.462 ± 0.114
1.569AspPhe: 1.569 ± 0.058
4.607AspGly: 4.607 ± 0.107
1.18AspHis: 1.18 ± 0.057
2.344AspIle: 2.344 ± 0.073
1.485AspLys: 1.485 ± 0.059
6.268AspLeu: 6.268 ± 0.126
0.914AspMet: 0.914 ± 0.04
0.777AspAsn: 0.777 ± 0.04
4.986AspPro: 4.986 ± 0.095
0.615AspGln: 0.615 ± 0.036
4.731AspArg: 4.731 ± 0.092
1.982AspSer: 1.982 ± 0.061
2.128AspThr: 2.128 ± 0.068
8.013AspVal: 8.013 ± 0.119
0.753AspTrp: 0.753 ± 0.046
1.573AspTyr: 1.573 ± 0.059
0.0AspXaa: 0.0 ± 0.0
Glu
7.677GluAla: 7.677 ± 0.134
1.152GluCys: 1.152 ± 0.048
5.947GluAsp: 5.947 ± 0.123
11.769GluGlu: 11.769 ± 0.226
3.186GluPhe: 3.186 ± 0.087
7.522GluGly: 7.522 ± 0.151
1.994GluHis: 1.994 ± 0.061
5.495GluIle: 5.495 ± 0.125
3.942GluLys: 3.942 ± 0.103
11.392GluLeu: 11.392 ± 0.195
1.827GluMet: 1.827 ± 0.062
1.87GluAsn: 1.87 ± 0.058
4.487GluPro: 4.487 ± 0.105
1.132GluGln: 1.132 ± 0.051
8.925GluArg: 8.925 ± 0.167
3.509GluSer: 3.509 ± 0.095
3.695GluThr: 3.695 ± 0.09
10.322GluVal: 10.322 ± 0.163
1.214GluTrp: 1.214 ± 0.053
2.942GluTyr: 2.942 ± 0.078
0.0GluXaa: 0.0 ± 0.0
Phe
2.064PheAla: 2.064 ± 0.069
0.393PheCys: 0.393 ± 0.027
1.926PheAsp: 1.926 ± 0.065
2.962PheGlu: 2.962 ± 0.086
0.771PhePhe: 0.771 ± 0.045
2.549PheGly: 2.549 ± 0.083
0.655PheHis: 0.655 ± 0.031
1.086PheIle: 1.086 ± 0.057
1.064PheLys: 1.064 ± 0.047
3.016PheLeu: 3.016 ± 0.091
0.451PheMet: 0.451 ± 0.027
0.645PheAsn: 0.645 ± 0.036
1.497PhePro: 1.497 ± 0.056
0.519PheGln: 0.519 ± 0.028
2.367PheArg: 2.367 ± 0.068
1.473PheSer: 1.473 ± 0.057
1.409PheThr: 1.409 ± 0.053
2.747PheVal: 2.747 ± 0.074
0.315PheTrp: 0.315 ± 0.025
0.818PheTyr: 0.818 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
6.248GlyAla: 6.248 ± 0.138
1.288GlyCys: 1.288 ± 0.05
4.857GlyAsp: 4.857 ± 0.106
7.152GlyGlu: 7.152 ± 0.12
2.861GlyPhe: 2.861 ± 0.084
6.118GlyGly: 6.118 ± 0.14
1.551GlyHis: 1.551 ± 0.054
4.493GlyIle: 4.493 ± 0.116
3.871GlyLys: 3.871 ± 0.096
7.404GlyLeu: 7.404 ± 0.141
1.942GlyMet: 1.942 ± 0.06
1.617GlyAsn: 1.617 ± 0.058
3.765GlyPro: 3.765 ± 0.089
1.094GlyGln: 1.094 ± 0.056
6.29GlyArg: 6.29 ± 0.13
4.164GlySer: 4.164 ± 0.081
4.14GlyThr: 4.14 ± 0.1
7.809GlyVal: 7.809 ± 0.134
1.216GlyTrp: 1.216 ± 0.052
2.633GlyTyr: 2.633 ± 0.078
0.0GlyXaa: 0.0 ± 0.0
His
1.705HisAla: 1.705 ± 0.069
0.327HisCys: 0.327 ± 0.029
1.048HisAsp: 1.048 ± 0.037
1.525HisGlu: 1.525 ± 0.047
0.473HisPhe: 0.473 ± 0.032
1.88HisGly: 1.88 ± 0.056
0.483HisHis: 0.483 ± 0.035
0.671HisIle: 0.671 ± 0.038
0.491HisLys: 0.491 ± 0.031
1.912HisLeu: 1.912 ± 0.055
0.355HisMet: 0.355 ± 0.027
0.351HisAsn: 0.351 ± 0.026
1.655HisPro: 1.655 ± 0.057
0.271HisGln: 0.271 ± 0.024
1.659HisArg: 1.659 ± 0.056
0.806HisSer: 0.806 ± 0.039
0.818HisThr: 0.818 ± 0.042
2.044HisVal: 2.044 ± 0.071
0.224HisTrp: 0.224 ± 0.019
0.613HisTyr: 0.613 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
4.633IleAla: 4.633 ± 0.103
0.591IleCys: 0.591 ± 0.041
2.98IleAsp: 2.98 ± 0.071
4.901IleGlu: 4.901 ± 0.111
1.024IlePhe: 1.024 ± 0.043
3.607IleGly: 3.607 ± 0.1
0.992IleHis: 0.992 ± 0.05
1.96IleIle: 1.96 ± 0.064
1.912IleLys: 1.912 ± 0.071
4.715IleLeu: 4.715 ± 0.118
0.818IleMet: 0.818 ± 0.043
0.884IleAsn: 0.884 ± 0.045
2.819IlePro: 2.819 ± 0.076
0.775IleGln: 0.775 ± 0.042
3.96IleArg: 3.96 ± 0.083
2.34IleSer: 2.34 ± 0.076
2.617IleThr: 2.617 ± 0.076
5.46IleVal: 5.46 ± 0.114
0.363IleTrp: 0.363 ± 0.028
0.916IleTyr: 0.916 ± 0.046
0.0IleXaa: 0.0 ± 0.0
Lys
3.449LysAla: 3.449 ± 0.093
0.463LysCys: 0.463 ± 0.033
2.094LysAsp: 2.094 ± 0.079
3.869LysGlu: 3.869 ± 0.096
1.22LysPhe: 1.22 ± 0.055
2.867LysGly: 2.867 ± 0.075
0.719LysHis: 0.719 ± 0.038
2.334LysIle: 2.334 ± 0.07
1.902LysLys: 1.902 ± 0.067
4.214LysLeu: 4.214 ± 0.106
0.773LysMet: 0.773 ± 0.043
0.747LysAsn: 0.747 ± 0.042
2.232LysPro: 2.232 ± 0.072
0.501LysGln: 0.501 ± 0.033
3.529LysArg: 3.529 ± 0.089
1.573LysSer: 1.573 ± 0.064
1.793LysThr: 1.793 ± 0.056
4.21LysVal: 4.21 ± 0.094
0.463LysTrp: 0.463 ± 0.035
1.276LysTyr: 1.276 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
9.498LeuAla: 9.498 ± 0.193
1.188LeuCys: 1.188 ± 0.05
6.001LeuAsp: 6.001 ± 0.125
10.526LeuGlu: 10.526 ± 0.144
2.102LeuPhe: 2.102 ± 0.073
8.53LeuGly: 8.53 ± 0.154
1.83LeuHis: 1.83 ± 0.069
4.37LeuIle: 4.37 ± 0.094
4.843LeuLys: 4.843 ± 0.118
9.656LeuLeu: 9.656 ± 0.196
1.821LeuMet: 1.821 ± 0.056
2.068LeuAsn: 2.068 ± 0.065
5.254LeuPro: 5.254 ± 0.118
1.605LeuGln: 1.605 ± 0.06
9.232LeuArg: 9.232 ± 0.158
5.286LeuSer: 5.286 ± 0.109
4.847LeuThr: 4.847 ± 0.111
8.627LeuVal: 8.627 ± 0.145
1.024LeuTrp: 1.024 ± 0.054
2.535LeuTyr: 2.535 ± 0.083
0.0LeuXaa: 0.0 ± 0.0
Met
1.627MetAla: 1.627 ± 0.054
0.252MetCys: 0.252 ± 0.02
1.08MetAsp: 1.08 ± 0.043
1.699MetGlu: 1.699 ± 0.061
0.553MetPhe: 0.553 ± 0.037
1.597MetGly: 1.597 ± 0.06
0.269MetHis: 0.269 ± 0.022
1.178MetIle: 1.178 ± 0.053
1.006MetLys: 1.006 ± 0.043
1.621MetLeu: 1.621 ± 0.053
0.409MetMet: 0.409 ± 0.032
0.437MetAsn: 0.437 ± 0.029
0.886MetPro: 0.886 ± 0.045
0.218MetGln: 0.218 ± 0.021
1.643MetArg: 1.643 ± 0.055
1.158MetSer: 1.158 ± 0.048
0.994MetThr: 0.994 ± 0.047
1.695MetVal: 1.695 ± 0.071
0.15MetTrp: 0.15 ± 0.019
0.525MetTyr: 0.525 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
1.749AsnAla: 1.749 ± 0.062
0.271AsnCys: 0.271 ± 0.024
0.856AsnAsp: 0.856 ± 0.041
1.304AsnGlu: 1.304 ± 0.059
0.601AsnPhe: 0.601 ± 0.036
1.317AsnGly: 1.317 ± 0.048
0.321AsnHis: 0.321 ± 0.026
0.932AsnIle: 0.932 ± 0.047
0.553AsnLys: 0.553 ± 0.034
2.048AsnLeu: 2.048 ± 0.065
0.371AsnMet: 0.371 ± 0.027
0.335AsnAsn: 0.335 ± 0.03
1.827AsnPro: 1.827 ± 0.07
0.261AsnGln: 0.261 ± 0.027
1.529AsnArg: 1.529 ± 0.05
0.717AsnSer: 0.717 ± 0.036
0.92AsnThr: 0.92 ± 0.042
2.557AsnVal: 2.557 ± 0.071
0.244AsnTrp: 0.244 ± 0.02
0.443AsnTyr: 0.443 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
3.713ProAla: 3.713 ± 0.086
0.613ProCys: 0.613 ± 0.032
3.881ProAsp: 3.881 ± 0.089
6.971ProGlu: 6.971 ± 0.123
1.595ProPhe: 1.595 ± 0.056
4.807ProGly: 4.807 ± 0.098
1.022ProHis: 1.022 ± 0.045
2.489ProIle: 2.489 ± 0.072
2.324ProLys: 2.324 ± 0.069
4.072ProLeu: 4.072 ± 0.095
1.016ProMet: 1.016 ± 0.05
1.18ProAsn: 1.18 ± 0.055
3.042ProPro: 3.042 ± 0.086
0.765ProGln: 0.765 ± 0.044
3.563ProArg: 3.563 ± 0.102
2.793ProSer: 2.793 ± 0.08
2.733ProThr: 2.733 ± 0.087
5.555ProVal: 5.555 ± 0.119
0.794ProTrp: 0.794 ± 0.042
1.701ProTyr: 1.701 ± 0.059
0.0ProXaa: 0.0 ± 0.0
Gln
1.238GlnAla: 1.238 ± 0.054
0.192GlnCys: 0.192 ± 0.021
0.723GlnAsp: 0.723 ± 0.035
1.16GlnGlu: 1.16 ± 0.049
0.417GlnPhe: 0.417 ± 0.028
1.17GlnGly: 1.17 ± 0.063
0.309GlnHis: 0.309 ± 0.025
0.701GlnIle: 0.701 ± 0.04
0.563GlnLys: 0.563 ± 0.036
1.517GlnLeu: 1.517 ± 0.057
0.275GlnMet: 0.275 ± 0.026
0.267GlnAsn: 0.267 ± 0.022
0.703GlnPro: 0.703 ± 0.045
0.311GlnGln: 0.311 ± 0.032
1.262GlnArg: 1.262 ± 0.058
0.609GlnSer: 0.609 ± 0.036
0.563GlnThr: 0.563 ± 0.031
1.421GlnVal: 1.421 ± 0.057
0.192GlnTrp: 0.192 ± 0.019
0.495GlnTyr: 0.495 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
6.727ArgAla: 6.727 ± 0.118
1.188ArgCys: 1.188 ± 0.049
4.298ArgAsp: 4.298 ± 0.107
9.139ArgGlu: 9.139 ± 0.156
2.499ArgPhe: 2.499 ± 0.079
6.172ArgGly: 6.172 ± 0.144
1.471ArgHis: 1.471 ± 0.052
4.398ArgIle: 4.398 ± 0.091
3.683ArgLys: 3.683 ± 0.085
7.691ArgLeu: 7.691 ± 0.156
1.88ArgMet: 1.88 ± 0.055
1.541ArgAsn: 1.541 ± 0.058
3.897ArgPro: 3.897 ± 0.086
1.07ArgGln: 1.07 ± 0.048
8.178ArgArg: 8.178 ± 0.166
3.581ArgSer: 3.581 ± 0.092
3.775ArgThr: 3.775 ± 0.088
8.424ArgVal: 8.424 ± 0.146
1.353ArgTrp: 1.353 ± 0.053
2.483ArgTyr: 2.483 ± 0.069
0.0ArgXaa: 0.0 ± 0.0
Ser
3.495SerAla: 3.495 ± 0.098
0.525SerCys: 0.525 ± 0.031
2.791SerAsp: 2.791 ± 0.079
4.531SerGlu: 4.531 ± 0.096
1.343SerPhe: 1.343 ± 0.049
4.513SerGly: 4.513 ± 0.09
0.908SerHis: 0.908 ± 0.043
1.9SerIle: 1.9 ± 0.07
1.667SerLys: 1.667 ± 0.06
4.332SerLeu: 4.332 ± 0.109
0.93SerMet: 0.93 ± 0.037
0.918SerAsn: 0.918 ± 0.05
2.831SerPro: 2.831 ± 0.086
0.699SerGln: 0.699 ± 0.038
3.507SerArg: 3.507 ± 0.094
2.539SerSer: 2.539 ± 0.096
2.276SerThr: 2.276 ± 0.077
4.659SerVal: 4.659 ± 0.107
0.643SerTrp: 0.643 ± 0.042
1.206SerTyr: 1.206 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
3.915ThrAla: 3.915 ± 0.092
0.647ThrCys: 0.647 ± 0.037
2.413ThrAsp: 2.413 ± 0.066
3.633ThrGlu: 3.633 ± 0.094
1.539ThrPhe: 1.539 ± 0.057
4.304ThrGly: 4.304 ± 0.098
0.844ThrHis: 0.844 ± 0.038
2.28ThrIle: 2.28 ± 0.064
1.371ThrLys: 1.371 ± 0.051
5.042ThrLeu: 5.042 ± 0.101
0.838ThrMet: 0.838 ± 0.042
0.8ThrAsn: 0.8 ± 0.045
2.735ThrPro: 2.735 ± 0.077
0.669ThrGln: 0.669 ± 0.037
2.819ThrArg: 2.819 ± 0.081
2.2ThrSer: 2.2 ± 0.058
2.29ThrThr: 2.29 ± 0.072
5.735ThrVal: 5.735 ± 0.118
0.617ThrTrp: 0.617 ± 0.037
1.254ThrTyr: 1.254 ± 0.052
0.0ThrXaa: 0.0 ± 0.0
Val
8.017ValAla: 8.017 ± 0.139
1.495ValCys: 1.495 ± 0.064
6.26ValAsp: 6.26 ± 0.119
10.889ValGlu: 10.889 ± 0.172
2.781ValPhe: 2.781 ± 0.071
7.574ValGly: 7.574 ± 0.138
2.004ValHis: 2.004 ± 0.073
5.354ValIle: 5.354 ± 0.12
4.763ValLys: 4.763 ± 0.106
10.805ValLeu: 10.805 ± 0.171
1.747ValMet: 1.747 ± 0.064
2.208ValAsn: 2.208 ± 0.064
5.515ValPro: 5.515 ± 0.121
1.551ValGln: 1.551 ± 0.056
8.893ValArg: 8.893 ± 0.148
5.0ValSer: 5.0 ± 0.12
5.018ValThr: 5.018 ± 0.122
10.71ValVal: 10.71 ± 0.171
1.016ValTrp: 1.016 ± 0.05
2.817ValTyr: 2.817 ± 0.068
0.0ValXaa: 0.0 ± 0.0
Trp
0.988TrpAla: 0.988 ± 0.052
0.214TrpCys: 0.214 ± 0.022
0.723TrpAsp: 0.723 ± 0.042
1.158TrpGlu: 1.158 ± 0.052
0.417TrpPhe: 0.417 ± 0.032
0.804TrpGly: 0.804 ± 0.039
0.158TrpHis: 0.158 ± 0.018
0.749TrpIle: 0.749 ± 0.046
0.727TrpLys: 0.727 ± 0.033
1.122TrpLeu: 1.122 ± 0.057
0.269TrpMet: 0.269 ± 0.022
0.365TrpAsn: 0.365 ± 0.026
0.501TrpPro: 0.501 ± 0.033
0.138TrpGln: 0.138 ± 0.017
0.974TrpArg: 0.974 ± 0.044
0.607TrpSer: 0.607 ± 0.035
0.607TrpThr: 0.607 ± 0.04
1.18TrpVal: 1.18 ± 0.05
0.301TrpTrp: 0.301 ± 0.024
0.391TrpTyr: 0.391 ± 0.03
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.226TyrAla: 2.226 ± 0.063
0.437TyrCys: 0.437 ± 0.032
1.844TyrAsp: 1.844 ± 0.066
2.379TyrGlu: 2.379 ± 0.073
0.814TyrPhe: 0.814 ± 0.035
2.517TyrGly: 2.517 ± 0.075
0.667TyrHis: 0.667 ± 0.038
0.98TyrIle: 0.98 ± 0.052
0.739TyrLys: 0.739 ± 0.039
3.16TyrLeu: 3.16 ± 0.09
0.451TyrMet: 0.451 ± 0.032
0.509TyrAsn: 0.509 ± 0.031
1.597TyrPro: 1.597 ± 0.061
0.485TyrGln: 0.485 ± 0.029
2.559TyrArg: 2.559 ± 0.073
1.417TyrSer: 1.417 ± 0.053
1.18TyrThr: 1.18 ± 0.051
2.878TyrVal: 2.878 ± 0.072
0.373TyrTrp: 0.373 ± 0.026
1.0TyrTyr: 1.0 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1687 proteins (500958 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski