Aureimonas altamirensis
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3519 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B1Q2E7|A0A0B1Q2E7_9RHIZ Uncharacterized protein OS=Aureimonas altamirensis OX=370622 GN=LA66_10610 PE=4 SV=1
MM1 pKa = 7.49 NIKK4 pKa = 10.39 SLLLGSAAALVAVSGARR21 pKa = 11.84 AADD24 pKa = 4.4 AIVMVEE30 pKa = 4.17 PEE32 pKa = 3.81 PVEE35 pKa = 3.97 YY36 pKa = 11.07 VRR38 pKa = 11.84 VCDD41 pKa = 3.81 VYY43 pKa = 10.81 GTGFYY48 pKa = 10.01 YY49 pKa = 9.98 IPGTEE54 pKa = 4.14 TCIKK58 pKa = 8.83 TSGYY62 pKa = 10.83 LRR64 pKa = 11.84 FQYY67 pKa = 10.64 NVAGSPAGNSEE78 pKa = 3.64 GDD80 pKa = 3.67 DD81 pKa = 3.8 YY82 pKa = 11.79 QALSSVRR89 pKa = 11.84 ARR91 pKa = 11.84 LNLDD95 pKa = 2.98 VRR97 pKa = 11.84 EE98 pKa = 4.01 EE99 pKa = 4.52 TEE101 pKa = 4.23 LGTLRR106 pKa = 11.84 AYY108 pKa = 10.36 FRR110 pKa = 11.84 LQAQNTGASPEE121 pKa = 4.53 DD122 pKa = 3.94 EE123 pKa = 4.21 FAMEE127 pKa = 4.25 QGYY130 pKa = 8.6 LQLGGFLAGYY140 pKa = 10.22 RR141 pKa = 11.84 DD142 pKa = 4.32 TLWSSDD148 pKa = 2.76 IGGIEE153 pKa = 4.96 DD154 pKa = 5.08 GLPTDD159 pKa = 4.89 DD160 pKa = 5.75 DD161 pKa = 4.69 LPIGDD166 pKa = 5.05 FNTNQISYY174 pKa = 8.46 TFATGGFAATLGLEE188 pKa = 4.38 DD189 pKa = 5.96 DD190 pKa = 4.38 GTGDD194 pKa = 3.63 IAPDD198 pKa = 3.34 VHH200 pKa = 6.86 GKK202 pKa = 10.3 LSYY205 pKa = 8.91 TGAWGGVFLSAVYY218 pKa = 10.68 DD219 pKa = 3.59 EE220 pKa = 4.89 TFNAEE225 pKa = 4.16 DD226 pKa = 3.5 VTDD229 pKa = 4.91 LFGIDD234 pKa = 5.57 DD235 pKa = 4.92 LDD237 pKa = 4.37 LPAFFPFIPEE247 pKa = 3.84 RR248 pKa = 11.84 DD249 pKa = 3.3 VDD251 pKa = 3.87 GSNDD255 pKa = 3.23 AFALKK260 pKa = 10.53 AGLTLQDD267 pKa = 4.62 LLVADD272 pKa = 4.2 STLKK276 pKa = 10.71 VDD278 pKa = 3.81 GHH280 pKa = 6.08 WASDD284 pKa = 3.44 PTSYY288 pKa = 11.4 AVISGFTSAGGILPSNATLEE308 pKa = 4.26 NPTPGAFPLVLEE320 pKa = 4.45 WSVGAGYY327 pKa = 8.38 SQKK330 pKa = 10.28 FNRR333 pKa = 11.84 VTAGVSGRR341 pKa = 11.84 YY342 pKa = 9.77 GEE344 pKa = 4.26 TFDD347 pKa = 3.88 YY348 pKa = 11.27 AFVAPEE354 pKa = 3.99 VPGGFVFSSADD365 pKa = 3.19 YY366 pKa = 8.63 WAVVGDD372 pKa = 3.74 IGYY375 pKa = 10.2 DD376 pKa = 3.1 LTANMNVLAEE386 pKa = 4.17 VKK388 pKa = 10.08 YY389 pKa = 10.88 QNIDD393 pKa = 3.37 FGGVASDD400 pKa = 4.04 DD401 pKa = 3.68 QTSGFLRR408 pKa = 11.84 FQRR411 pKa = 11.84 NFF413 pKa = 3.06
Molecular weight: 44.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.846
IPC_protein 3.872
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.215
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.986
Patrickios 1.901
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.885
Protein with the highest isoelectric point:
>tr|A0A0B1Q233|A0A0B1Q233_9RHIZ DNA-3-methyladenine glycosidase OS=Aureimonas altamirensis OX=370622 GN=LA66_19850 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 8.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATTGGRR28 pKa = 11.84 RR29 pKa = 11.84 VIASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.73 GRR39 pKa = 11.84 KK40 pKa = 8.92 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3519
0
3519
1115690
26
2158
317.0
34.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.011 ± 0.058
0.76 ± 0.012
5.846 ± 0.038
5.653 ± 0.04
3.785 ± 0.03
8.925 ± 0.033
1.973 ± 0.02
5.197 ± 0.028
2.525 ± 0.029
10.154 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.587 ± 0.021
2.335 ± 0.021
5.141 ± 0.027
2.973 ± 0.023
7.558 ± 0.036
5.344 ± 0.028
5.214 ± 0.029
7.604 ± 0.032
1.235 ± 0.013
2.178 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here