Burkholderia sp. Leaf177

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; unclassified Burkholderia

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5955 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5QCS7|A0A0Q5QCS7_9BURK Arsenate reductase OS=Burkholderia sp. Leaf177 OX=1736287 GN=ASG35_03400 PE=3 SV=1
MM1 pKa = 7.9SEE3 pKa = 3.9VLEE6 pKa = 4.24YY7 pKa = 10.77KK8 pKa = 10.32SWVCLICGWIYY19 pKa = 11.0NEE21 pKa = 4.11EE22 pKa = 4.61DD23 pKa = 3.62GLPEE27 pKa = 5.29DD28 pKa = 5.57GIAAGTRR35 pKa = 11.84FADD38 pKa = 4.8IPDD41 pKa = 3.37TWRR44 pKa = 11.84CPLCDD49 pKa = 3.28VGKK52 pKa = 9.89EE53 pKa = 3.91DD54 pKa = 4.28FVAVEE59 pKa = 4.23FF60 pKa = 4.67

Molecular weight: 6.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5QAB5|A0A0Q5QAB5_9BURK Ribosome-recycling factor OS=Burkholderia sp. Leaf177 OX=1736287 GN=frr PE=3 SV=1
MM1 pKa = 7.93AIGVHH6 pKa = 5.61HH7 pKa = 6.58VGIVLRR13 pKa = 11.84VLLVLPVLLAVASVVRR29 pKa = 11.84SRR31 pKa = 11.84RR32 pKa = 11.84VTMLQTVIAVRR43 pKa = 11.84GLRR46 pKa = 11.84SAIVAHH52 pKa = 6.52RR53 pKa = 11.84AVIVLRR59 pKa = 11.84DD60 pKa = 3.75PRR62 pKa = 11.84AMASAVRR69 pKa = 11.84SRR71 pKa = 11.84RR72 pKa = 11.84VAMLQEE78 pKa = 4.36VIAVRR83 pKa = 11.84GLRR86 pKa = 11.84LAIVVLRR93 pKa = 11.84AVIVLRR99 pKa = 11.84VLRR102 pKa = 11.84AKK104 pKa = 10.76ASVVRR109 pKa = 11.84SRR111 pKa = 11.84LAVMRR116 pKa = 11.84QGVIGGRR123 pKa = 11.84GLRR126 pKa = 11.84SAIVAHH132 pKa = 6.52RR133 pKa = 11.84AVIVLRR139 pKa = 11.84ILPAMVSVVRR149 pKa = 11.84SRR151 pKa = 11.84RR152 pKa = 11.84VVMRR156 pKa = 11.84QGVNAVHH163 pKa = 7.08GLRR166 pKa = 11.84SAIVAHH172 pKa = 6.52RR173 pKa = 11.84AVIVLRR179 pKa = 11.84VLPAMASVDD188 pKa = 3.17RR189 pKa = 11.84SRR191 pKa = 11.84RR192 pKa = 11.84VVMRR196 pKa = 11.84HH197 pKa = 5.09VMIADD202 pKa = 3.91RR203 pKa = 11.84GLRR206 pKa = 11.84SAIAALHH213 pKa = 4.77VVIVLRR219 pKa = 11.84DD220 pKa = 3.43LQAIANVVHH229 pKa = 6.43SALATTRR236 pKa = 11.84RR237 pKa = 11.84VAQAAIARR245 pKa = 11.84RR246 pKa = 11.84ALLSTTAVVRR256 pKa = 11.84AVRR259 pKa = 11.84APTAAHH265 pKa = 5.9VLLSATAAHH274 pKa = 6.34LVVIAQAVTANAARR288 pKa = 11.84SNRR291 pKa = 11.84GKK293 pKa = 10.38IPVAQEE299 pKa = 3.81AIVRR303 pKa = 11.84RR304 pKa = 11.84VRR306 pKa = 11.84RR307 pKa = 11.84STTTAHH313 pKa = 7.13LAASATTHH321 pKa = 4.74TMAARR326 pKa = 11.84VRR328 pKa = 11.84PLSTANVRR336 pKa = 11.84QATVLTRR343 pKa = 11.84NARR346 pKa = 11.84HH347 pKa = 5.94RR348 pKa = 11.84RR349 pKa = 11.84NASAMSANPHH359 pKa = 6.49ANRR362 pKa = 11.84LQNWQNPATPTHH374 pKa = 6.43HH375 pKa = 6.93RR376 pKa = 11.84HH377 pKa = 6.06PPASPAKK384 pKa = 10.66VKK386 pKa = 10.22MEE388 pKa = 3.68WCAYY392 pKa = 9.91RR393 pKa = 11.84KK394 pKa = 9.8

Molecular weight: 42.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5955

0

5955

1921382

24

3195

322.7

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.149 ± 0.037

0.869 ± 0.011

5.495 ± 0.023

5.174 ± 0.028

3.836 ± 0.023

8.037 ± 0.034

2.247 ± 0.016

5.108 ± 0.02

3.53 ± 0.026

10.077 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.46 ± 0.016

3.074 ± 0.021

4.923 ± 0.02

3.484 ± 0.019

6.494 ± 0.03

6.045 ± 0.025

5.594 ± 0.023

7.707 ± 0.024

1.321 ± 0.014

2.377 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski