Rhodospirillales bacterium URHD0017

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; unclassified Rhodospirillales

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7953 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8YBL7|A0A1H8YBL7_9PROT DNA-binding transcriptional regulator LysR family OS=Rhodospirillales bacterium URHD0017 OX=1380357 GN=SAMN02990966_07307 PE=3 SV=1
MM1 pKa = 7.25ATLNVGAGQAYY12 pKa = 6.91ATLSAAVASSHH23 pKa = 6.91DD24 pKa = 3.53GDD26 pKa = 4.43VIAVQAGTYY35 pKa = 10.39VNDD38 pKa = 3.71FATIFNDD45 pKa = 3.25VTIVGVGGMANFVATVAPPNGKK67 pKa = 10.28GILVTQGDD75 pKa = 3.99VTIQNLSFSGVAVADD90 pKa = 3.96ANGAGIRR97 pKa = 11.84YY98 pKa = 9.09EE99 pKa = 4.17GGNLVVIDD107 pKa = 5.19SYY109 pKa = 11.92FHH111 pKa = 7.17DD112 pKa = 3.59NQMNLLANAADD123 pKa = 3.69GTIRR127 pKa = 11.84IVGSEE132 pKa = 4.27FGATRR137 pKa = 11.84SSDD140 pKa = 3.73SLNHH144 pKa = 5.87NLYY147 pKa = 10.18VGSIGSLVIDD157 pKa = 3.31NSYY160 pKa = 10.83FHH162 pKa = 7.5DD163 pKa = 5.13AYY165 pKa = 10.6DD166 pKa = 3.4GHH168 pKa = 6.0QIKK171 pKa = 10.48SRR173 pKa = 11.84AEE175 pKa = 3.81STTITNSRR183 pKa = 11.84IYY185 pKa = 10.5DD186 pKa = 3.53GSGDD190 pKa = 3.49GSYY193 pKa = 10.65TIDD196 pKa = 4.09LPNGGVGVISNNVIEE211 pKa = 4.67QGPHH215 pKa = 5.53SDD217 pKa = 3.32NPFIIAYY224 pKa = 8.82SEE226 pKa = 4.02EE227 pKa = 4.57GATHH231 pKa = 7.25ASNSLVIEE239 pKa = 4.61DD240 pKa = 3.58NVVVNDD246 pKa = 3.65LTGNASLLFNASTITGTIRR265 pKa = 11.84DD266 pKa = 3.69NSVYY270 pKa = 10.81GLTADD275 pKa = 3.62QIVYY279 pKa = 10.55GSATVSGTHH288 pKa = 6.26FLSTHH293 pKa = 5.82PTLDD297 pKa = 3.28TTSGVGGGGTGGGGTGGGGDD317 pKa = 3.48GGGGGSAAIGEE328 pKa = 4.59VTGLSQDD335 pKa = 3.06TGTPGDD341 pKa = 4.91FITSVASQTVHH352 pKa = 4.49GTYY355 pKa = 10.6AGTLANSDD363 pKa = 4.37VIQVSADD370 pKa = 2.7GSTWVGATAGAGTWTADD387 pKa = 3.33VTLLPGEE394 pKa = 4.16HH395 pKa = 6.03TLAVRR400 pKa = 11.84TVDD403 pKa = 3.62DD404 pKa = 4.67AGNIVNGTGHH414 pKa = 7.5AYY416 pKa = 10.27DD417 pKa = 4.84LEE419 pKa = 4.67TGGGGDD425 pKa = 3.66GTVLGTAGADD435 pKa = 3.59TLTGTAGADD444 pKa = 3.32TMTGFAGNDD453 pKa = 3.15EE454 pKa = 4.4YY455 pKa = 11.88VVNNVGDD462 pKa = 3.8VVIEE466 pKa = 3.89ARR468 pKa = 11.84GEE470 pKa = 4.18GTDD473 pKa = 3.49TVLSSVSYY481 pKa = 10.46RR482 pKa = 11.84LAWNQSIEE490 pKa = 4.01NLTLTGDD497 pKa = 3.81GNIDD501 pKa = 3.35GTGNSMNNVLQGSAGDD517 pKa = 3.57NVLRR521 pKa = 11.84GGSGNDD527 pKa = 3.42TLSGGDD533 pKa = 3.68GNDD536 pKa = 3.25VLVGGFGRR544 pKa = 11.84DD545 pKa = 3.18IEE547 pKa = 4.54TGGAGADD554 pKa = 2.91RR555 pKa = 11.84FDD557 pKa = 4.26FNSVNEE563 pKa = 4.31SGTTFATRR571 pKa = 11.84EE572 pKa = 3.85QIMGFEE578 pKa = 4.2QGTDD582 pKa = 3.49AIDD585 pKa = 4.18FSTIDD590 pKa = 3.72ANTAAWGNQAFTFIGDD606 pKa = 3.79DD607 pKa = 3.51AFHH610 pKa = 6.42GVAGEE615 pKa = 4.04LHH617 pKa = 5.68QQTDD621 pKa = 4.03GPNTIVSGDD630 pKa = 3.42INGDD634 pKa = 3.52SVADD638 pKa = 3.89FQIEE642 pKa = 4.22LNGAFTLTPNDD653 pKa = 4.29FILL656 pKa = 5.08

Molecular weight: 66.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8U8S1|A0A1H8U8S1_9PROT Uncharacterized protein OS=Rhodospirillales bacterium URHD0017 OX=1380357 GN=SAMN02990966_03594 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.63GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.31VIASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84SGRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.09

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7953

0

7953

2442273

39

2555

307.1

33.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.515 ± 0.038

0.901 ± 0.008

5.535 ± 0.022

5.186 ± 0.024

3.685 ± 0.015

8.723 ± 0.036

2.079 ± 0.014

4.764 ± 0.018

3.562 ± 0.028

10.025 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.489 ± 0.013

2.574 ± 0.017

5.568 ± 0.021

3.137 ± 0.016

7.3 ± 0.031

5.137 ± 0.02

5.263 ± 0.021

7.691 ± 0.023

1.552 ± 0.011

2.314 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski