Candidatus Izimaplasma sp. ZiA1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Tenericutes incertae sedis; Candidatus Izimaplasma; unclassified Candidatus Izimaplasma

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1828 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A1ZTL8|A0A2A1ZTL8_9BACT 50S ribosomal protein L11 OS=Candidatus Izimaplasma sp. ZiA1 OX=2024899 GN=rplK PE=3 SV=1
MM1 pKa = 7.8DD2 pKa = 6.7LIEE5 pKa = 4.49EE6 pKa = 4.9MIPEE10 pKa = 4.54ANVSTTYY17 pKa = 11.36DD18 pKa = 3.01MSSFQDD24 pKa = 3.89AIVEE28 pKa = 4.3MVAQTRR34 pKa = 11.84NGVLGVVAQEE44 pKa = 4.36LSGSTVIGGGTGSGVVYY61 pKa = 10.52KK62 pKa = 10.71KK63 pKa = 11.05VGDD66 pKa = 3.62VYY68 pKa = 11.45YY69 pKa = 10.7LVTNAHH75 pKa = 6.71VITEE79 pKa = 4.46DD80 pKa = 3.41VTDD83 pKa = 3.58TLGQVTGTEE92 pKa = 3.97LLDD95 pKa = 3.65NFVITYY101 pKa = 7.5EE102 pKa = 4.24KK103 pKa = 11.29NNLLFNINEE112 pKa = 4.29VTVVGYY118 pKa = 10.72DD119 pKa = 3.36LTTDD123 pKa = 4.28LAVLTFTSDD132 pKa = 2.92EE133 pKa = 4.21YY134 pKa = 11.36FSVIEE139 pKa = 4.69FGDD142 pKa = 3.7SYY144 pKa = 11.35AIEE147 pKa = 4.63PGQFVFAIGNPLGFNYY163 pKa = 10.58YY164 pKa = 8.62GTLTMGVISGTSRR177 pKa = 11.84YY178 pKa = 9.47LQDD181 pKa = 3.36GDD183 pKa = 4.34FDD185 pKa = 4.08ATLLQHH191 pKa = 7.1DD192 pKa = 4.4AAISPGNSGGALVDD206 pKa = 3.62INGKK210 pKa = 10.1LIGINNMKK218 pKa = 10.18IVDD221 pKa = 3.97ADD223 pKa = 3.54VTGIGFAIPSNTVQRR238 pKa = 11.84IVEE241 pKa = 4.2DD242 pKa = 4.23LEE244 pKa = 5.88DD245 pKa = 4.77DD246 pKa = 4.33GVVTRR251 pKa = 11.84PFLGIVTSAQVNQCGIDD268 pKa = 3.52YY269 pKa = 8.96GVCLLDD275 pKa = 5.27SEE277 pKa = 4.84SQPAIYY283 pKa = 10.01PNGAADD289 pKa = 3.71NAEE292 pKa = 4.24LQSGDD297 pKa = 3.75VIIGFKK303 pKa = 10.66KK304 pKa = 9.76IDD306 pKa = 3.32QEE308 pKa = 4.47EE309 pKa = 4.59YY310 pKa = 10.68FDD312 pKa = 3.95VFNFNDD318 pKa = 3.67LKK320 pKa = 11.08EE321 pKa = 5.18AILNCKK327 pKa = 10.21VGDD330 pKa = 3.91QIQIKK335 pKa = 8.71YY336 pKa = 10.12IRR338 pKa = 11.84DD339 pKa = 3.59NEE341 pKa = 4.22IKK343 pKa = 10.19EE344 pKa = 4.43SNIVTLGVHH353 pKa = 6.85PDD355 pKa = 3.49DD356 pKa = 4.49

Molecular weight: 38.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A1ZNL1|A0A2A1ZNL1_9BACT Uncharacterized protein OS=Candidatus Izimaplasma sp. ZiA1 OX=2024899 GN=CI105_09305 PE=4 SV=1
MM1 pKa = 6.89PAAWKK6 pKa = 10.18GRR8 pKa = 11.84SGRR11 pKa = 11.84VWGKK15 pKa = 9.17RR16 pKa = 11.84GKK18 pKa = 9.4WPVKK22 pKa = 9.85PGLTFHH28 pKa = 7.31IDD30 pKa = 3.04DD31 pKa = 3.84SAYY34 pKa = 8.68FQYY37 pKa = 10.74RR38 pKa = 11.84RR39 pKa = 11.84IVRR42 pKa = 11.84SWRR45 pKa = 11.84IADD48 pKa = 3.48ARR50 pKa = 11.84SEE52 pKa = 4.25EE53 pKa = 4.2NPQNRR58 pKa = 11.84AWRR61 pKa = 11.84IRR63 pKa = 11.84SAKK66 pKa = 10.21KK67 pKa = 9.4MLKK70 pKa = 10.37CSDD73 pKa = 3.54RR74 pKa = 11.84ALSEE78 pKa = 4.01VRR80 pKa = 11.84GTNEE84 pKa = 4.04WISEE88 pKa = 4.08ANTFRR93 pKa = 11.84IIAYY97 pKa = 9.12LAAGGCEE104 pKa = 4.41VYY106 pKa = 10.66SAA108 pKa = 4.4

Molecular weight: 12.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1828

0

1828

575450

37

2306

314.8

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.072 ± 0.057

0.735 ± 0.018

5.99 ± 0.051

6.875 ± 0.053

4.957 ± 0.047

5.863 ± 0.055

1.625 ± 0.021

9.738 ± 0.069

8.397 ± 0.071

9.837 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.028

6.334 ± 0.07

2.619 ± 0.03

2.367 ± 0.026

3.183 ± 0.038

6.427 ± 0.05

5.497 ± 0.043

6.866 ± 0.047

0.543 ± 0.014

4.588 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski