Flavivirga eckloniae
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4547 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K9PUU9|A0A2K9PUU9_9FLAO Adenylosuccinate lyase OS=Flavivirga eckloniae OX=1803846 GN=C1H87_19875 PE=4 SV=1
MM1 pKa = 7.44 SKK3 pKa = 9.83 IFKK6 pKa = 10.07 ILFIFPIAVTMLLISCRR23 pKa = 11.84 TEE25 pKa = 3.54 EE26 pKa = 4.38 TEE28 pKa = 5.37 LIEE31 pKa = 4.76 APEE34 pKa = 4.28 DD35 pKa = 3.96 EE36 pKa = 4.42 ILVANSKK43 pKa = 9.76 IANLMLKK50 pKa = 9.33 TSSNDD55 pKa = 3.0 GSFDD59 pKa = 4.37 NIIDD63 pKa = 3.85 KK64 pKa = 11.08 SNCFNINFPLAVTANNQQINVSSKK88 pKa = 10.05 SDD90 pKa = 3.48 YY91 pKa = 10.99 KK92 pKa = 10.43 IIEE95 pKa = 4.46 YY96 pKa = 10.31 IFDD99 pKa = 4.2 EE100 pKa = 6.13 DD101 pKa = 6.18 DD102 pKa = 5.07 DD103 pKa = 4.51 DD104 pKa = 4.99 TDD106 pKa = 4.26 ALDD109 pKa = 3.12 ITFPIKK115 pKa = 10.32 ISLKK119 pKa = 8.02 DD120 pKa = 3.47 HH121 pKa = 6.66 SVVTVNNYY129 pKa = 8.84 TEE131 pKa = 4.28 LSSYY135 pKa = 11.52 SNNCNGEE142 pKa = 3.86 NEE144 pKa = 3.87 QDD146 pKa = 4.91 DD147 pKa = 5.65 DD148 pKa = 5.32 IEE150 pKa = 4.79 CLDD153 pKa = 3.75 FKK155 pKa = 11.57 YY156 pKa = 10.34 PITASIFNTNNEE168 pKa = 4.4 LIDD171 pKa = 3.94 VKK173 pKa = 9.95 TLNSDD178 pKa = 3.13 SSLYY182 pKa = 10.78 EE183 pKa = 3.75 LVDD186 pKa = 3.55 NLNVSDD192 pKa = 4.66 FVTINFPITVTLLDD206 pKa = 3.79 GTEE209 pKa = 4.18 LNVNNLTEE217 pKa = 4.5 LEE219 pKa = 4.44 TIIEE223 pKa = 4.54 RR224 pKa = 11.84 YY225 pKa = 10.51 NNDD228 pKa = 3.26 CDD230 pKa = 4.66 EE231 pKa = 5.61 DD232 pKa = 4.79 DD233 pKa = 5.61 DD234 pKa = 5.55 YY235 pKa = 11.84 DD236 pKa = 5.58 YY237 pKa = 12.0 NDD239 pKa = 5.49 DD240 pKa = 5.46 DD241 pKa = 6.71 CDD243 pKa = 4.15 DD244 pKa = 4.45 CDD246 pKa = 4.89 RR247 pKa = 11.84 EE248 pKa = 4.12 QLTDD252 pKa = 3.5 VLTNCADD259 pKa = 3.24 WTVDD263 pKa = 3.76 KK264 pKa = 11.37 LEE266 pKa = 4.81 RR267 pKa = 11.84 DD268 pKa = 3.72 NNDD271 pKa = 3.46 LDD273 pKa = 4.5 NIYY276 pKa = 10.96 DD277 pKa = 4.3 GYY279 pKa = 9.77 TFNFFTDD286 pKa = 3.97 GTLSVYY292 pKa = 9.21 WPSRR296 pKa = 11.84 TVYY299 pKa = 8.62 GTWVANGTGNNITVTINVPDD319 pKa = 4.42 LPYY322 pKa = 11.09 CNNDD326 pKa = 3.63 WILHH330 pKa = 5.89 EE331 pKa = 4.11 ISKK334 pKa = 8.56 YY335 pKa = 9.48 TEE337 pKa = 3.94 TKK339 pKa = 9.35 VDD341 pKa = 3.8 LRR343 pKa = 11.84 VGGIDD348 pKa = 3.69 RR349 pKa = 11.84 LRR351 pKa = 11.84 YY352 pKa = 10.34 DD353 pKa = 3.52 NGCNN357 pKa = 3.37
Molecular weight: 40.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.706
IPC_protein 3.745
Toseland 3.503
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.567
Grimsley 3.414
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.126
Thurlkill 3.567
EMBOSS 3.694
Sillero 3.859
Patrickios 0.909
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A2K9PWG0|A0A2K9PWG0_9FLAO Uncharacterized protein OS=Flavivirga eckloniae OX=1803846 GN=C1H87_22815 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.92 GRR40 pKa = 11.84 KK41 pKa = 8.34 KK42 pKa = 10.21 ISVSSEE48 pKa = 3.74 TRR50 pKa = 11.84 HH51 pKa = 5.96 KK52 pKa = 10.68 KK53 pKa = 9.8
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4547
0
4547
1645158
32
4712
361.8
40.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.961 ± 0.043
0.779 ± 0.017
5.779 ± 0.036
6.301 ± 0.038
5.219 ± 0.031
6.415 ± 0.042
1.796 ± 0.019
8.03 ± 0.037
7.882 ± 0.056
9.041 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.988 ± 0.018
6.755 ± 0.036
3.376 ± 0.024
3.084 ± 0.02
3.295 ± 0.024
6.754 ± 0.03
6.066 ± 0.047
6.107 ± 0.029
1.149 ± 0.014
4.224 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here