Thalassotalea sp. HSM 43

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae; Thalassotalea; unclassified Thalassotalea

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3411 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P7JJL3|A0A4P7JJL3_9GAMM Uncharacterized protein OS=Thalassotalea sp. HSM 43 OX=2552945 GN=E2K93_01510 PE=4 SV=1
MM1 pKa = 7.57SKK3 pKa = 10.44NLNKK7 pKa = 10.33SYY9 pKa = 10.79LALLVASALSLTACGGGGGGGGTEE33 pKa = 4.23KK34 pKa = 10.99KK35 pKa = 10.42EE36 pKa = 4.13IEE38 pKa = 4.56PPTNNAPTISGSPSLSINEE57 pKa = 3.97NQSYY61 pKa = 10.78SFSANGADD69 pKa = 3.82SDD71 pKa = 4.39GDD73 pKa = 3.75ALTYY77 pKa = 10.61SIANMPQWAAFDD89 pKa = 4.15SATGTLTGTPGNADD103 pKa = 2.93VGTFTGITISVSDD116 pKa = 3.95GTDD119 pKa = 3.08SSSLSPFDD127 pKa = 5.22IIVNDD132 pKa = 3.75IFALQGSATLEE143 pKa = 4.16VTVGEE148 pKa = 4.62TNVFTPTLVDD158 pKa = 3.83HH159 pKa = 6.87VDD161 pKa = 3.12ADD163 pKa = 4.14FSYY166 pKa = 10.92SIDD169 pKa = 3.68GAPSFAGFSFDD180 pKa = 3.62AATGQVSVTPAADD193 pKa = 4.64DD194 pKa = 3.38IGSFNITITASNGVNSASHH213 pKa = 6.33NMTLTISPGSISVAGKK229 pKa = 10.7VIDD232 pKa = 4.54GYY234 pKa = 10.91ISGAHH239 pKa = 5.75VFLDD243 pKa = 3.64VNNNMMFDD251 pKa = 4.05DD252 pKa = 4.72NEE254 pKa = 4.21PNALSNNAGEE264 pKa = 4.44FTLDD268 pKa = 3.61VPGTLIDD275 pKa = 4.79SIPTSTLVAEE285 pKa = 4.98LGSGQGVDD293 pKa = 3.81ADD295 pKa = 3.92DD296 pKa = 3.87MSRR299 pKa = 11.84FDD301 pKa = 5.27DD302 pKa = 4.78DD303 pKa = 3.73FAARR307 pKa = 11.84PIKK310 pKa = 9.93MMSYY314 pKa = 10.17PIRR317 pKa = 11.84DD318 pKa = 3.94LTNMSNGLTAYY329 pKa = 7.37ITPFTHH335 pKa = 7.74LIHH338 pKa = 7.05EE339 pKa = 5.29EE340 pKa = 3.86ILSAAGGDD348 pKa = 3.54LSLITDD354 pKa = 3.46QEE356 pKa = 4.67LYY358 pKa = 11.37NLIKK362 pKa = 10.74DD363 pKa = 3.98SAVDD367 pKa = 4.77RR368 pKa = 11.84INGLYY373 pKa = 10.59SVDD376 pKa = 4.66LDD378 pKa = 4.25LATSDD383 pKa = 3.91FFAAEE388 pKa = 3.97VPINSRR394 pKa = 11.84NDD396 pKa = 3.0IASVAKK402 pKa = 10.48AVLNDD407 pKa = 4.09LQDD410 pKa = 3.41QVNDD414 pKa = 3.54SDD416 pKa = 4.06QDD418 pKa = 3.76GFVNAEE424 pKa = 4.2DD425 pKa = 4.55DD426 pKa = 4.14LPFNSEE432 pKa = 4.41YY433 pKa = 10.48ISDD436 pKa = 3.76IDD438 pKa = 3.82QDD440 pKa = 4.03GLGDD444 pKa = 4.17CVRR447 pKa = 11.84TIPGKK452 pKa = 10.4SCVEE456 pKa = 3.99EE457 pKa = 6.17DD458 pKa = 5.23DD459 pKa = 6.62DD460 pKa = 5.98IDD462 pKa = 5.19GDD464 pKa = 4.29NITNADD470 pKa = 4.0DD471 pKa = 4.35PEE473 pKa = 4.36PTVPAGLAVFKK484 pKa = 9.66PVPEE488 pKa = 4.18MNVPGYY494 pKa = 8.65EE495 pKa = 3.96MCVGLVNQDD504 pKa = 3.71KK505 pKa = 10.81VLCLDD510 pKa = 3.97NYY512 pKa = 9.74QWSILDD518 pKa = 4.01SIKK521 pKa = 11.07GEE523 pKa = 4.12PLPIDD528 pKa = 3.43TNSIVKK534 pKa = 10.52SGDD537 pKa = 2.96SAAAVGTCAVANNEE551 pKa = 4.23VYY553 pKa = 10.84CLDD556 pKa = 3.07GKK558 pKa = 10.07MRR560 pKa = 11.84FDD562 pKa = 3.93FAGEE566 pKa = 4.14NPVSLNPIALEE577 pKa = 3.74MGYY580 pKa = 11.17LSMCYY585 pKa = 9.95LDD587 pKa = 3.65QTGVKK592 pKa = 9.64CFGGIEE598 pKa = 3.99EE599 pKa = 4.92SMPEE603 pKa = 4.08SLVNASALALTSGNACAISEE623 pKa = 4.36VTDD626 pKa = 3.47NEE628 pKa = 4.32QTSTAVSCWGSDD640 pKa = 3.24SYY642 pKa = 12.09GLTTGVPVLSNPTQLFSNMTTICALDD668 pKa = 3.58DD669 pKa = 3.66TGIKK673 pKa = 10.1CWGNPDD679 pKa = 3.56YY680 pKa = 11.49NLDD683 pKa = 4.01KK684 pKa = 10.92VDD686 pKa = 3.69HH687 pKa = 6.9ASTKK691 pKa = 10.37LSNIHH696 pKa = 5.89LTGDD700 pKa = 3.59SLCFIDD706 pKa = 5.23EE707 pKa = 4.22QTPVCYY713 pKa = 10.47GNSDD717 pKa = 3.57QPTPISADD725 pKa = 2.96KK726 pKa = 10.89DD727 pKa = 3.84YY728 pKa = 11.45FDD730 pKa = 5.64ADD732 pKa = 3.56SGEE735 pKa = 4.78LIVAHH740 pKa = 6.52GEE742 pKa = 4.13FIAPFEE748 pKa = 4.23EE749 pKa = 4.37QLTKK753 pKa = 10.79YY754 pKa = 10.66VEE756 pKa = 4.63GVHH759 pKa = 4.77QQRR762 pKa = 11.84VLLPEE767 pKa = 3.75VDD769 pKa = 4.53FEE771 pKa = 4.4QGQTATEE778 pKa = 4.17VFRR781 pKa = 11.84LRR783 pKa = 11.84NYY785 pKa = 7.95TKK787 pKa = 9.15NTCLISDD794 pKa = 4.54GEE796 pKa = 4.29ISCDD800 pKa = 3.26SQRR803 pKa = 11.84YY804 pKa = 8.73CGLDD808 pKa = 3.13NGEE811 pKa = 4.26WQCQTVEE818 pKa = 4.48FNFDD822 pKa = 4.21DD823 pKa = 3.65IDD825 pKa = 4.54FEE827 pKa = 5.24VKK829 pKa = 9.02TQINNSNTVCAIGALKK845 pKa = 9.95AHH847 pKa = 6.47CVGAGFNGEE856 pKa = 3.86IKK858 pKa = 10.94LNN860 pKa = 3.7

Molecular weight: 91.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P7JQM4|A0A4P7JQM4_9GAMM UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2 6-diaminopimelate ligase OS=Thalassotalea sp. HSM 43 OX=2552945 GN=murE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.33NGRR28 pKa = 11.84AVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.41GRR39 pKa = 11.84AKK41 pKa = 10.57LSSS44 pKa = 3.31

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3411

0

3411

1170031

38

2158

343.0

38.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.691 ± 0.044

0.995 ± 0.014

6.223 ± 0.04

5.791 ± 0.036

4.292 ± 0.025

6.731 ± 0.042

2.263 ± 0.021

6.527 ± 0.03

5.386 ± 0.038

9.743 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.642 ± 0.019

4.692 ± 0.029

3.705 ± 0.021

5.077 ± 0.041

4.117 ± 0.033

6.54 ± 0.032

5.162 ± 0.033

6.917 ± 0.034

1.22 ± 0.016

3.287 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski