Streptococcus phage Javan381

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ADZ0|A0A4D6ADZ0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan381 OX=2548138 GN=Javan381_0011 PE=4 SV=1
MM1 pKa = 7.52SVQQSIVNGFTSRR14 pKa = 11.84RR15 pKa = 11.84GLITYY20 pKa = 10.8SMFGSRR26 pKa = 11.84NGSDD30 pKa = 3.19GTGDD34 pKa = 3.3CSGIMSQVLKK44 pKa = 10.42EE45 pKa = 3.88AGINIIGLPSTVTLGQQLANNGFYY69 pKa = 10.21RR70 pKa = 11.84VSINQDD76 pKa = 2.07WDD78 pKa = 3.54AQPADD83 pKa = 4.57IILMSWGADD92 pKa = 3.06MSTSGGDD99 pKa = 3.5GGHH102 pKa = 6.08VGAMIDD108 pKa = 3.28DD109 pKa = 4.65TYY111 pKa = 11.13FISCDD116 pKa = 3.41YY117 pKa = 9.38STQGATGQAINTYY130 pKa = 8.88PWNDD134 pKa = 3.24YY135 pKa = 10.81YY136 pKa = 11.66GWNKK140 pKa = 8.83PAYY143 pKa = 8.57IEE145 pKa = 3.88VWRR148 pKa = 11.84YY149 pKa = 10.53ADD151 pKa = 3.87TAPQTDD157 pKa = 2.99NRR159 pKa = 11.84ANTTVQPQDD168 pKa = 2.98KK169 pKa = 10.2AYY171 pKa = 10.97YY172 pKa = 8.15EE173 pKa = 4.31ANEE176 pKa = 3.96VKK178 pKa = 10.25YY179 pKa = 11.21VNGIWQIKK187 pKa = 9.1CDD189 pKa = 3.74YY190 pKa = 9.01LAPVGFDD197 pKa = 2.79WTEE200 pKa = 3.54NGIPVSMVNWVDD212 pKa = 3.19KK213 pKa = 11.14DD214 pKa = 3.99GNNLPDD220 pKa = 4.54GADD223 pKa = 3.45KK224 pKa = 11.0DD225 pKa = 4.54FKK227 pKa = 11.3SGMFFSFEE235 pKa = 4.12LDD237 pKa = 3.27EE238 pKa = 4.71AHH240 pKa = 6.75ITDD243 pKa = 3.86TGKK246 pKa = 10.07GGYY249 pKa = 9.11YY250 pKa = 10.14GGYY253 pKa = 7.62YY254 pKa = 8.88WRR256 pKa = 11.84LFEE259 pKa = 5.13FGQFGPIWLSCWDD272 pKa = 3.98KK273 pKa = 11.8DD274 pKa = 4.5DD275 pKa = 4.37LVNYY279 pKa = 9.92YY280 pKa = 9.54EE281 pKa = 4.09

Molecular weight: 31.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A8W7|A0A4D6A8W7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan381 OX=2548138 GN=Javan381_0046 PE=4 SV=1
MM1 pKa = 7.12TRR3 pKa = 11.84SKK5 pKa = 10.9NVEE8 pKa = 3.94GVALEE13 pKa = 4.42LFMSKK18 pKa = 10.51NSVYY22 pKa = 10.78RR23 pKa = 11.84NVIKK27 pKa = 10.5PFFEE31 pKa = 4.32GLEE34 pKa = 4.19KK35 pKa = 10.63KK36 pKa = 8.94VTSIFLKK43 pKa = 10.1TRR45 pKa = 3.31

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

11543

37

1566

240.5

27.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.823 ± 0.791

0.468 ± 0.084

5.856 ± 0.407

8.221 ± 0.734

4.002 ± 0.234

6.809 ± 0.36

1.256 ± 0.192

6.324 ± 0.248

7.97 ± 0.456

8.49 ± 0.309

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.469 ± 0.219

5.527 ± 0.307

2.911 ± 0.295

4.765 ± 0.375

4.349 ± 0.419

5.908 ± 0.664

6.047 ± 0.382

5.986 ± 0.223

1.213 ± 0.163

3.604 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski