Ramlibacter sp. H242

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Ramlibacter; unclassified Ramlibacter

Average proteome isoelectric point is 7.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2805 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M5XSG0|A0A6M5XSG0_9BURK PhnD/SsuA/transferrin family substrate-binding protein OS=Ramlibacter sp. H242 OX=2732511 GN=HK414_10795 PE=4 SV=1
MM1 pKa = 7.87DD2 pKa = 3.47VHH4 pKa = 7.34AGRR7 pKa = 11.84RR8 pKa = 11.84LPRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84QLHH16 pKa = 4.55GHH18 pKa = 4.57RR19 pKa = 11.84HH20 pKa = 4.34RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84QRR26 pKa = 11.84RR27 pKa = 11.84HH28 pKa = 5.14PVISLAVTAVADD40 pKa = 4.0IVADD44 pKa = 3.75TASVNEE50 pKa = 4.4DD51 pKa = 2.92ASVTTNLLANDD62 pKa = 3.9SFEE65 pKa = 4.2GAEE68 pKa = 4.29TITAVTQGTHH78 pKa = 4.17GTVTIVDD85 pKa = 3.95PALGTVLYY93 pKa = 10.08TPDD96 pKa = 4.34ANWHH100 pKa = 5.25GTDD103 pKa = 3.06TYY105 pKa = 10.48TYY107 pKa = 8.81TVTSGGVSEE116 pKa = 4.42TATVTVTVAQVNDD129 pKa = 3.67PATFGGATSGSGNEE143 pKa = 4.26DD144 pKa = 3.3DD145 pKa = 5.19AAITGTLTAADD156 pKa = 5.31AIDD159 pKa = 4.62GMTTPAFTVTGAAAHH174 pKa = 5.72GTATINAATGAWSYY188 pKa = 10.91TPAADD193 pKa = 4.23YY194 pKa = 11.36NGTDD198 pKa = 3.15SFTVRR203 pKa = 11.84VTDD206 pKa = 3.32NDD208 pKa = 4.07GNFATQVITLNVASVADD225 pKa = 3.85IAADD229 pKa = 3.54TASVNEE235 pKa = 4.66DD236 pKa = 3.01GSVTSNLLANDD247 pKa = 3.73SFEE250 pKa = 4.2GAEE253 pKa = 4.29TITAVTQGANGTVSIVDD270 pKa = 4.05GAAGTVLYY278 pKa = 9.63TPNANFHH285 pKa = 5.71GTDD288 pKa = 3.06TYY290 pKa = 11.02TYY292 pKa = 8.81TVTSGGVTEE301 pKa = 4.4TATVTVTVAQVDD313 pKa = 4.02DD314 pKa = 3.96PATFGGATSGTGNEE328 pKa = 4.04DD329 pKa = 2.89AAAITGTLTAADD341 pKa = 5.31AIDD344 pKa = 4.62GMTTPAFTVVGAAAHH359 pKa = 6.7GSATINAATGAWSYY373 pKa = 10.91TPAADD378 pKa = 3.86YY379 pKa = 10.75NGPDD383 pKa = 3.31SFTVRR388 pKa = 11.84VTDD391 pKa = 3.81NNGNTATQVISLTVTAVADD410 pKa = 3.94IVADD414 pKa = 3.66TATVNEE420 pKa = 4.57DD421 pKa = 3.58ANVTTNLLANDD432 pKa = 3.9SFEE435 pKa = 4.2GAEE438 pKa = 4.4TITSVTQGTNGTVTILDD455 pKa = 3.81PVLGTVRR462 pKa = 11.84YY463 pKa = 6.97TPNANFHH470 pKa = 5.71GTDD473 pKa = 3.06TYY475 pKa = 11.02TYY477 pKa = 8.81TVTSGGVSEE486 pKa = 4.42TATVTVTVAQVDD498 pKa = 4.02DD499 pKa = 3.96PATFGGATSGSGSEE513 pKa = 4.25DD514 pKa = 2.87AVAITGTLTATDD526 pKa = 5.36AIDD529 pKa = 4.78GMTTPAFTVTGAAAHH544 pKa = 5.72GTATINATTGAWSYY558 pKa = 10.88TPAADD563 pKa = 3.86YY564 pKa = 10.75NGPDD568 pKa = 3.31SFTVRR573 pKa = 11.84VTDD576 pKa = 3.81NNGNTATRR584 pKa = 11.84VISLTVTAVADD595 pKa = 3.92IVADD599 pKa = 3.75TASVNEE605 pKa = 4.41DD606 pKa = 3.17SSVTTNLLTNDD617 pKa = 3.52SFEE620 pKa = 4.21GAEE623 pKa = 4.29TITAVTQGTNGTVTIVNAALGTVLYY648 pKa = 9.59TPNADD653 pKa = 3.33FHH655 pKa = 6.53GTDD658 pKa = 3.06TYY660 pKa = 11.04TYY662 pKa = 8.81TVTSGGVTEE671 pKa = 4.4TATVTVTVAQVDD683 pKa = 4.02DD684 pKa = 3.96PATFGGATTGTGSEE698 pKa = 4.49DD699 pKa = 3.12GAAITGTLTASDD711 pKa = 5.31AIDD714 pKa = 4.12GMTTPAFTVIGNGAHH729 pKa = 7.11GSATINASTGVWSYY743 pKa = 11.23TPVADD748 pKa = 4.1YY749 pKa = 10.96NGPDD753 pKa = 3.33SFTVRR758 pKa = 11.84VTDD761 pKa = 3.81NNGNTATRR769 pKa = 11.84VISLTVTAVADD780 pKa = 3.94IVADD784 pKa = 3.66TATVNEE790 pKa = 4.6DD791 pKa = 3.69SNVTTNLLANDD802 pKa = 4.03TFEE805 pKa = 4.29GAEE808 pKa = 4.25TITAVTQGANGTVTILDD825 pKa = 3.81PVLGTVRR832 pKa = 11.84YY833 pKa = 6.97TPNANFHH840 pKa = 5.71GTDD843 pKa = 3.06TYY845 pKa = 11.02TYY847 pKa = 8.81TVTSGGVSEE856 pKa = 4.42TATVTVTVAQVDD868 pKa = 4.02DD869 pKa = 3.96PATFGGATTGTGNEE883 pKa = 4.18DD884 pKa = 3.26AATITGTLTASDD896 pKa = 5.31AIDD899 pKa = 4.12GMTTPAFTVTGAAAHH914 pKa = 5.72GTATINATTGAWSYY928 pKa = 11.07TPVADD933 pKa = 4.21YY934 pKa = 11.44NGADD938 pKa = 3.39SFTVRR943 pKa = 11.84VTDD946 pKa = 3.81NNGNTATQVITLTVTAAADD965 pKa = 3.56IVADD969 pKa = 3.79TASVNEE975 pKa = 4.41DD976 pKa = 3.17SSVTTNLLTNDD987 pKa = 3.52SFEE990 pKa = 4.21GAEE993 pKa = 4.29TITAVTQGTNGTVTIVNAALGTVLYY1018 pKa = 9.51TPNANFHH1025 pKa = 5.71GTDD1028 pKa = 3.06TYY1030 pKa = 11.02TYY1032 pKa = 8.81TVTSGGVTEE1041 pKa = 4.4TATVTVTVNQVDD1053 pKa = 3.95DD1054 pKa = 3.86PATFGGATTGSGSEE1068 pKa = 4.42DD1069 pKa = 3.11GAAITGTLTASDD1081 pKa = 5.31AIDD1084 pKa = 4.12GMTTPAFTVIGNGAHH1099 pKa = 7.11GSATINASTGVWSYY1113 pKa = 11.23TPVADD1118 pKa = 4.21YY1119 pKa = 11.44NGADD1123 pKa = 3.39SFTVRR1128 pKa = 11.84VTDD1131 pKa = 3.81NNGNTATQVISLSVTAVADD1150 pKa = 3.79IVADD1154 pKa = 3.75TASVNEE1160 pKa = 4.42DD1161 pKa = 3.42ANVTTNLLANDD1172 pKa = 4.03TFEE1175 pKa = 4.29GAEE1178 pKa = 4.25TITAVTQGTNGTVTILDD1195 pKa = 3.81PVLGTVRR1202 pKa = 11.84YY1203 pKa = 6.97TPNANFHH1210 pKa = 5.71GTDD1213 pKa = 3.06TYY1215 pKa = 11.02TYY1217 pKa = 8.81TVTSGGVSEE1226 pKa = 4.42TATVTVTVARR1236 pKa = 11.84STTPPPSAAPRR1247 pKa = 11.84AAAAMKK1253 pKa = 8.34TLPRR1257 pKa = 11.84SRR1259 pKa = 11.84ARR1261 pKa = 3.48

Molecular weight: 126.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M5XVB6|A0A6M5XVB6_9BURK Uncharacterized protein OS=Ramlibacter sp. H242 OX=2732511 GN=HK414_19700 PE=4 SV=1
MM1 pKa = 6.84QRR3 pKa = 11.84KK4 pKa = 6.25TFHH7 pKa = 6.85RR8 pKa = 11.84FLLAAAAAATLGWGSARR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84SPRR30 pKa = 11.84FRR32 pKa = 11.84SSTRR36 pKa = 11.84WPSAARR42 pKa = 11.84SRR44 pKa = 11.84RR45 pKa = 11.84PSTAMPPTSS54 pKa = 3.31

Molecular weight: 6.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2805

0

2805

608434

33

1265

216.9

23.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.802 ± 0.072

1.162 ± 0.019

5.07 ± 0.044

4.964 ± 0.046

3.136 ± 0.031

8.938 ± 0.065

2.243 ± 0.029

3.557 ± 0.036

2.724 ± 0.044

9.537 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.371 ± 0.027

2.338 ± 0.031

5.969 ± 0.049

3.489 ± 0.037

8.571 ± 0.076

5.542 ± 0.04

5.631 ± 0.092

7.641 ± 0.045

1.426 ± 0.024

1.889 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski