Amino acid dipepetide frequency for Ramlibacter sp. H242

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.582AlaAla: 21.582 ± 0.277
1.808AlaCys: 1.808 ± 0.059
6.63AlaAsp: 6.63 ± 0.118
6.367AlaGlu: 6.367 ± 0.108
3.849AlaPhe: 3.849 ± 0.083
13.817AlaGly: 13.817 ± 0.175
2.904AlaHis: 2.904 ± 0.072
4.725AlaIle: 4.725 ± 0.092
3.382AlaLys: 3.382 ± 0.097
13.426AlaLeu: 13.426 ± 0.191
3.632AlaMet: 3.632 ± 0.093
2.894AlaAsn: 2.894 ± 0.088
7.657AlaPro: 7.657 ± 0.157
5.401AlaGln: 5.401 ± 0.109
11.457AlaArg: 11.457 ± 0.183
7.784AlaSer: 7.784 ± 0.137
7.432AlaThr: 7.432 ± 0.166
9.421AlaVal: 9.421 ± 0.152
1.75AlaTrp: 1.75 ± 0.057
2.096AlaTyr: 2.096 ± 0.062
0.0AlaXaa: 0.0 ± 0.0
Cys
1.714CysAla: 1.714 ± 0.057
0.416CysCys: 0.416 ± 0.027
0.491CysAsp: 0.491 ± 0.031
0.526CysGlu: 0.526 ± 0.034
0.273CysPhe: 0.273 ± 0.02
1.162CysGly: 1.162 ± 0.047
0.251CysHis: 0.251 ± 0.021
0.37CysIle: 0.37 ± 0.027
0.219CysLys: 0.219 ± 0.021
0.672CysLeu: 0.672 ± 0.036
0.284CysMet: 0.284 ± 0.023
0.242CysAsn: 0.242 ± 0.019
0.672CysPro: 0.672 ± 0.035
0.156CysGln: 0.156 ± 0.016
1.354CysArg: 1.354 ± 0.052
0.919CysSer: 0.919 ± 0.044
0.661CysThr: 0.661 ± 0.033
0.702CysVal: 0.702 ± 0.034
0.393CysTrp: 0.393 ± 0.026
0.141CysTyr: 0.141 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
7.524AspAla: 7.524 ± 0.129
0.422AspCys: 0.422 ± 0.027
2.801AspAsp: 2.801 ± 0.068
2.59AspGlu: 2.59 ± 0.064
1.939AspPhe: 1.939 ± 0.061
5.057AspGly: 5.057 ± 0.12
1.04AspHis: 1.04 ± 0.041
2.119AspIle: 2.119 ± 0.066
1.326AspLys: 1.326 ± 0.051
4.577AspLeu: 4.577 ± 0.097
1.077AspMet: 1.077 ± 0.04
1.167AspAsn: 1.167 ± 0.048
3.039AspPro: 3.039 ± 0.068
1.205AspGln: 1.205 ± 0.048
3.974AspArg: 3.974 ± 0.1
2.104AspSer: 2.104 ± 0.076
2.625AspThr: 2.625 ± 0.084
4.148AspVal: 4.148 ± 0.083
0.835AspTrp: 0.835 ± 0.037
1.134AspTyr: 1.134 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
6.696GluAla: 6.696 ± 0.112
0.347GluCys: 0.347 ± 0.023
2.418GluAsp: 2.418 ± 0.074
2.561GluGlu: 2.561 ± 0.085
1.617GluPhe: 1.617 ± 0.061
3.894GluGly: 3.894 ± 0.076
1.165GluHis: 1.165 ± 0.044
2.104GluIle: 2.104 ± 0.06
1.542GluLys: 1.542 ± 0.057
5.662GluLeu: 5.662 ± 0.114
1.257GluMet: 1.257 ± 0.04
1.04GluAsn: 1.04 ± 0.043
2.488GluPro: 2.488 ± 0.067
2.286GluGln: 2.286 ± 0.057
4.664GluArg: 4.664 ± 0.098
2.171GluSer: 2.171 ± 0.066
2.319GluThr: 2.319 ± 0.063
3.89GluVal: 3.89 ± 0.073
0.649GluTrp: 0.649 ± 0.034
0.873GluTyr: 0.873 ± 0.039
0.0GluXaa: 0.0 ± 0.0
Phe
4.089PheAla: 4.089 ± 0.091
0.339PheCys: 0.339 ± 0.024
2.275PheAsp: 2.275 ± 0.06
1.89PheGlu: 1.89 ± 0.054
1.006PhePhe: 1.006 ± 0.04
3.116PheGly: 3.116 ± 0.072
0.754PheHis: 0.754 ± 0.041
1.203PheIle: 1.203 ± 0.047
0.825PheLys: 0.825 ± 0.041
2.288PheLeu: 2.288 ± 0.059
0.662PheMet: 0.662 ± 0.034
0.846PheAsn: 0.846 ± 0.037
1.652PhePro: 1.652 ± 0.056
0.689PheGln: 0.689 ± 0.033
2.247PheArg: 2.247 ± 0.062
1.841PheSer: 1.841 ± 0.056
2.013PheThr: 2.013 ± 0.076
2.556PheVal: 2.556 ± 0.07
0.426PheTrp: 0.426 ± 0.026
0.641PheTyr: 0.641 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
11.201GlyAla: 11.201 ± 0.179
1.152GlyCys: 1.152 ± 0.048
4.523GlyAsp: 4.523 ± 0.096
4.495GlyGlu: 4.495 ± 0.083
3.096GlyPhe: 3.096 ± 0.075
8.711GlyGly: 8.711 ± 0.178
2.035GlyHis: 2.035 ± 0.059
3.627GlyIle: 3.627 ± 0.076
2.835GlyLys: 2.835 ± 0.068
7.815GlyLeu: 7.815 ± 0.138
2.289GlyMet: 2.289 ± 0.061
2.745GlyAsn: 2.745 ± 0.101
3.798GlyPro: 3.798 ± 0.085
2.718GlyGln: 2.718 ± 0.062
7.156GlyArg: 7.156 ± 0.131
5.429GlySer: 5.429 ± 0.119
5.715GlyThr: 5.715 ± 0.183
6.292GlyVal: 6.292 ± 0.112
1.635GlyTrp: 1.635 ± 0.06
2.11GlyTyr: 2.11 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
3.238HisAla: 3.238 ± 0.079
0.274HisCys: 0.274 ± 0.022
1.029HisAsp: 1.029 ± 0.043
1.083HisGlu: 1.083 ± 0.041
0.817HisPhe: 0.817 ± 0.038
2.447HisGly: 2.447 ± 0.084
0.694HisHis: 0.694 ± 0.04
0.741HisIle: 0.741 ± 0.034
0.486HisLys: 0.486 ± 0.027
1.865HisLeu: 1.865 ± 0.06
0.488HisMet: 0.488 ± 0.026
0.339HisAsn: 0.339 ± 0.022
1.634HisPro: 1.634 ± 0.06
0.669HisGln: 0.669 ± 0.033
2.266HisArg: 2.266 ± 0.078
0.884HisSer: 0.884 ± 0.043
0.855HisThr: 0.855 ± 0.039
1.708HisVal: 1.708 ± 0.062
0.437HisTrp: 0.437 ± 0.03
0.472HisTyr: 0.472 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.353IleAla: 5.353 ± 0.093
0.307IleCys: 0.307 ± 0.023
2.557IleAsp: 2.557 ± 0.058
2.419IleGlu: 2.419 ± 0.07
1.003IlePhe: 1.003 ± 0.04
3.461IleGly: 3.461 ± 0.085
0.748IleHis: 0.748 ± 0.041
1.009IleIle: 1.009 ± 0.044
0.973IleLys: 0.973 ± 0.049
2.559IleLeu: 2.559 ± 0.064
0.496IleMet: 0.496 ± 0.03
1.004IleAsn: 1.004 ± 0.049
1.765IlePro: 1.765 ± 0.058
0.731IleGln: 0.731 ± 0.039
2.548IleArg: 2.548 ± 0.06
1.908IleSer: 1.908 ± 0.06
2.493IleThr: 2.493 ± 0.087
3.092IleVal: 3.092 ± 0.077
0.376IleTrp: 0.376 ± 0.028
0.768IleTyr: 0.768 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
3.626LysAla: 3.626 ± 0.11
0.169LysCys: 0.169 ± 0.017
1.336LysAsp: 1.336 ± 0.059
1.34LysGlu: 1.34 ± 0.05
0.771LysPhe: 0.771 ± 0.039
2.031LysGly: 2.031 ± 0.063
0.557LysHis: 0.557 ± 0.029
1.016LysIle: 1.016 ± 0.04
1.109LysLys: 1.109 ± 0.058
3.049LysLeu: 3.049 ± 0.071
0.699LysMet: 0.699 ± 0.034
0.648LysAsn: 0.648 ± 0.04
1.936LysPro: 1.936 ± 0.062
1.029LysGln: 1.029 ± 0.04
1.987LysArg: 1.987 ± 0.058
1.346LysSer: 1.346 ± 0.05
1.515LysThr: 1.515 ± 0.05
2.204LysVal: 2.204 ± 0.068
0.385LysTrp: 0.385 ± 0.027
0.486LysTyr: 0.486 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
13.513LeuAla: 13.513 ± 0.181
0.914LeuCys: 0.914 ± 0.038
4.765LeuAsp: 4.765 ± 0.094
4.594LeuGlu: 4.594 ± 0.093
2.884LeuPhe: 2.884 ± 0.074
7.307LeuGly: 7.307 ± 0.122
2.378LeuHis: 2.378 ± 0.063
2.723LeuIle: 2.723 ± 0.082
2.814LeuLys: 2.814 ± 0.079
10.496LeuLeu: 10.496 ± 0.167
2.056LeuMet: 2.056 ± 0.062
2.128LeuAsn: 2.128 ± 0.058
6.007LeuPro: 6.007 ± 0.115
4.314LeuGln: 4.314 ± 0.09
8.346LeuArg: 8.346 ± 0.145
5.074LeuSer: 5.074 ± 0.095
4.707LeuThr: 4.707 ± 0.097
7.463LeuVal: 7.463 ± 0.132
1.188LeuTrp: 1.188 ± 0.049
1.701LeuTyr: 1.701 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
3.082MetAla: 3.082 ± 0.084
0.184MetCys: 0.184 ± 0.021
1.123MetAsp: 1.123 ± 0.044
1.192MetGlu: 1.192 ± 0.045
0.674MetPhe: 0.674 ± 0.033
1.759MetGly: 1.759 ± 0.062
0.537MetHis: 0.537 ± 0.032
0.731MetIle: 0.731 ± 0.037
0.911MetLys: 0.911 ± 0.044
2.641MetLeu: 2.641 ± 0.067
0.557MetMet: 0.557 ± 0.031
0.728MetAsn: 0.728 ± 0.036
1.627MetPro: 1.627 ± 0.053
1.123MetGln: 1.123 ± 0.047
1.928MetArg: 1.928 ± 0.058
1.251MetSer: 1.251 ± 0.042
1.471MetThr: 1.471 ± 0.053
1.688MetVal: 1.688 ± 0.053
0.194MetTrp: 0.194 ± 0.016
0.306MetTyr: 0.306 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
3.202AsnAla: 3.202 ± 0.087
0.247AsnCys: 0.247 ± 0.023
1.455AsnAsp: 1.455 ± 0.072
1.093AsnGlu: 1.093 ± 0.049
0.868AsnPhe: 0.868 ± 0.045
2.336AsnGly: 2.336 ± 0.099
0.426AsnHis: 0.426 ± 0.028
0.945AsnIle: 0.945 ± 0.041
0.689AsnLys: 0.689 ± 0.036
2.105AsnLeu: 2.105 ± 0.059
0.485AsnMet: 0.485 ± 0.028
0.61AsnAsn: 0.61 ± 0.037
1.54AsnPro: 1.54 ± 0.054
0.652AsnGln: 0.652 ± 0.035
1.56AsnArg: 1.56 ± 0.047
1.062AsnSer: 1.062 ± 0.051
1.333AsnThr: 1.333 ± 0.05
1.852AsnVal: 1.852 ± 0.056
0.403AsnTrp: 0.403 ± 0.023
0.521AsnTyr: 0.521 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
9.51ProAla: 9.51 ± 0.16
0.628ProCys: 0.628 ± 0.034
3.249ProAsp: 3.249 ± 0.086
3.335ProGlu: 3.335 ± 0.078
1.668ProPhe: 1.668 ± 0.052
5.877ProGly: 5.877 ± 0.094
1.318ProHis: 1.318 ± 0.053
1.578ProIle: 1.578 ± 0.056
1.348ProLys: 1.348 ± 0.052
4.793ProLeu: 4.793 ± 0.111
1.326ProMet: 1.326 ± 0.05
1.284ProAsn: 1.284 ± 0.048
3.976ProPro: 3.976 ± 0.116
2.059ProGln: 2.059 ± 0.061
4.75ProArg: 4.75 ± 0.119
3.42ProSer: 3.42 ± 0.077
3.001ProThr: 3.001 ± 0.074
4.926ProVal: 4.926 ± 0.088
0.598ProTrp: 0.598 ± 0.029
1.049ProTyr: 1.049 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
4.978GlnAla: 4.978 ± 0.116
0.281GlnCys: 0.281 ± 0.026
1.436GlnAsp: 1.436 ± 0.048
1.353GlnGlu: 1.353 ± 0.05
1.008GlnPhe: 1.008 ± 0.035
2.477GlnGly: 2.477 ± 0.064
0.912GlnHis: 0.912 ± 0.036
1.096GlnIle: 1.096 ± 0.04
1.016GlnLys: 1.016 ± 0.051
3.92GlnLeu: 3.92 ± 0.089
0.822GlnMet: 0.822 ± 0.039
0.626GlnAsn: 0.626 ± 0.035
2.569GlnPro: 2.569 ± 0.086
1.861GlnGln: 1.861 ± 0.074
3.69GlnArg: 3.69 ± 0.083
1.732GlnSer: 1.732 ± 0.056
1.331GlnThr: 1.331 ± 0.047
2.597GlnVal: 2.597 ± 0.075
0.62GlnTrp: 0.62 ± 0.037
0.567GlnTyr: 0.567 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
10.493ArgAla: 10.493 ± 0.174
1.496ArgCys: 1.496 ± 0.058
3.871ArgAsp: 3.871 ± 0.093
4.567ArgGlu: 4.567 ± 0.097
2.735ArgPhe: 2.735 ± 0.065
6.559ArgGly: 6.559 ± 0.127
2.298ArgHis: 2.298 ± 0.086
3.243ArgIle: 3.243 ± 0.079
2.289ArgLys: 2.289 ± 0.068
7.736ArgLeu: 7.736 ± 0.112
2.485ArgMet: 2.485 ± 0.068
1.938ArgAsn: 1.938 ± 0.058
5.319ArgPro: 5.319 ± 0.122
2.924ArgGln: 2.924 ± 0.078
9.506ArgArg: 9.506 ± 0.223
5.13ArgSer: 5.13 ± 0.119
4.295ArgThr: 4.295 ± 0.087
5.509ArgVal: 5.509 ± 0.086
1.711ArgTrp: 1.711 ± 0.057
1.607ArgTyr: 1.607 ± 0.052
0.0ArgXaa: 0.0 ± 0.0
Ser
7.212SerAla: 7.212 ± 0.112
0.769SerCys: 0.769 ± 0.036
2.311SerAsp: 2.311 ± 0.07
2.344SerGlu: 2.344 ± 0.064
1.902SerPhe: 1.902 ± 0.058
5.718SerGly: 5.718 ± 0.118
1.017SerHis: 1.017 ± 0.043
2.16SerIle: 2.16 ± 0.057
1.284SerLys: 1.284 ± 0.045
4.661SerLeu: 4.661 ± 0.09
1.43SerMet: 1.43 ± 0.052
1.195SerAsn: 1.195 ± 0.05
3.688SerPro: 3.688 ± 0.081
1.43SerGln: 1.43 ± 0.052
4.655SerArg: 4.655 ± 0.091
3.958SerSer: 3.958 ± 0.099
3.708SerThr: 3.708 ± 0.102
4.073SerVal: 4.073 ± 0.088
0.708SerTrp: 0.708 ± 0.04
1.198SerTyr: 1.198 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
7.199ThrAla: 7.199 ± 0.182
0.753ThrCys: 0.753 ± 0.036
2.564ThrAsp: 2.564 ± 0.087
2.488ThrGlu: 2.488 ± 0.074
1.716ThrPhe: 1.716 ± 0.057
5.644ThrGly: 5.644 ± 0.128
1.063ThrHis: 1.063 ± 0.04
2.216ThrIle: 2.216 ± 0.081
1.05ThrLys: 1.05 ± 0.045
5.6ThrLeu: 5.6 ± 0.112
1.269ThrMet: 1.269 ± 0.044
1.073ThrAsn: 1.073 ± 0.051
3.949ThrPro: 3.949 ± 0.112
1.579ThrGln: 1.579 ± 0.052
4.214ThrArg: 4.214 ± 0.084
3.609ThrSer: 3.609 ± 0.093
3.433ThrThr: 3.433 ± 0.107
5.253ThrVal: 5.253 ± 0.245
0.444ThrTrp: 0.444 ± 0.03
1.192ThrTyr: 1.192 ± 0.064
0.0ThrXaa: 0.0 ± 0.0
Val
10.057ValAla: 10.057 ± 0.126
0.626ValCys: 0.626 ± 0.035
4.226ValAsp: 4.226 ± 0.083
3.977ValGlu: 3.977 ± 0.075
2.303ValPhe: 2.303 ± 0.067
5.48ValGly: 5.48 ± 0.094
1.601ValHis: 1.601 ± 0.054
2.873ValIle: 2.873 ± 0.081
2.097ValLys: 2.097 ± 0.064
8.21ValLeu: 8.21 ± 0.134
1.632ValMet: 1.632 ± 0.059
1.985ValAsn: 1.985 ± 0.062
4.392ValPro: 4.392 ± 0.092
2.975ValGln: 2.975 ± 0.075
6.106ValArg: 6.106 ± 0.084
3.915ValSer: 3.915 ± 0.081
5.148ValThr: 5.148 ± 0.232
6.635ValVal: 6.635 ± 0.122
0.861ValTrp: 0.861 ± 0.039
1.313ValTyr: 1.313 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
1.356TrpAla: 1.356 ± 0.047
0.289TrpCys: 0.289 ± 0.02
0.514TrpAsp: 0.514 ± 0.033
0.468TrpGlu: 0.468 ± 0.028
0.483TrpPhe: 0.483 ± 0.032
0.846TrpGly: 0.846 ± 0.043
0.325TrpHis: 0.325 ± 0.023
0.531TrpIle: 0.531 ± 0.03
0.486TrpLys: 0.486 ± 0.029
1.67TrpLeu: 1.67 ± 0.054
0.432TrpMet: 0.432 ± 0.027
0.337TrpAsn: 0.337 ± 0.028
1.035TrpPro: 1.035 ± 0.044
0.572TrpGln: 0.572 ± 0.031
1.637TrpArg: 1.637 ± 0.053
0.975TrpSer: 0.975 ± 0.041
0.797TrpThr: 0.797 ± 0.039
0.886TrpVal: 0.886 ± 0.038
0.363TrpTrp: 0.363 ± 0.028
0.255TrpTyr: 0.255 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.393TyrAla: 2.393 ± 0.073
0.197TyrCys: 0.197 ± 0.018
1.131TyrAsp: 1.131 ± 0.056
0.961TyrGlu: 0.961 ± 0.04
0.746TyrPhe: 0.746 ± 0.039
1.678TyrGly: 1.678 ± 0.058
0.401TyrHis: 0.401 ± 0.03
0.562TyrIle: 0.562 ± 0.03
0.557TyrLys: 0.557 ± 0.032
1.629TyrLeu: 1.629 ± 0.061
0.324TyrMet: 0.324 ± 0.023
0.552TyrAsn: 0.552 ± 0.034
0.948TyrPro: 0.948 ± 0.034
0.518TyrGln: 0.518 ± 0.031
1.665TyrArg: 1.665 ± 0.057
0.911TyrSer: 0.911 ± 0.042
1.456TyrThr: 1.456 ± 0.113
1.517TyrVal: 1.517 ± 0.049
0.283TyrTrp: 0.283 ± 0.024
0.46TyrTyr: 0.46 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2805 proteins (608434 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski