Paenibacillus sp. DMB20

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4674 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M2VXV5|A0A0M2VXV5_9BACL CoxE OS=Paenibacillus sp. DMB20 OX=1642570 GN=XI25_01660 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.7LMPEE6 pKa = 4.09YY7 pKa = 10.76AQILIVGVVFNGTWSWYY24 pKa = 6.63ITEE27 pKa = 4.02RR28 pKa = 11.84EE29 pKa = 3.94YY30 pKa = 10.81WFLNIEE36 pKa = 3.94MEE38 pKa = 4.17DD39 pKa = 3.47RR40 pKa = 11.84FGIEE44 pKa = 3.9VLNEE48 pKa = 3.69TTAEE52 pKa = 4.12TFLDD56 pKa = 3.7QIVDD60 pKa = 3.92YY61 pKa = 10.55KK62 pKa = 11.43VSTSEE67 pKa = 3.95LTEE70 pKa = 3.8MLVDD74 pKa = 4.82LNSAFQSFDD83 pKa = 3.4DD84 pKa = 3.76VLEE87 pKa = 4.04YY88 pKa = 10.58MPTIYY93 pKa = 11.07VNFDD97 pKa = 3.01DD98 pKa = 6.09RR99 pKa = 11.84IFYY102 pKa = 10.84SLFPEE107 pKa = 4.32PMSFEE112 pKa = 5.09DD113 pKa = 4.27YY114 pKa = 11.02VPKK117 pKa = 10.63GWVGEE122 pKa = 4.12YY123 pKa = 10.39KK124 pKa = 10.54DD125 pKa = 4.64FYY127 pKa = 11.22NIVPDD132 pKa = 3.8QEE134 pKa = 4.45QYY136 pKa = 10.4WIVDD140 pKa = 3.28GDD142 pKa = 4.13HH143 pKa = 7.22LFDD146 pKa = 5.06CLWKK150 pKa = 10.36RR151 pKa = 11.84FKK153 pKa = 11.06GVV155 pKa = 2.82

Molecular weight: 18.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M2W0C1|A0A0M2W0C1_9BACL Membrane protein OS=Paenibacillus sp. DMB20 OX=1642570 GN=XI25_00620 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 9.45QTFKK6 pKa = 11.13PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4674

0

4674

1355747

26

4052

290.1

32.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.075 ± 0.037

0.817 ± 0.012

5.187 ± 0.028

6.967 ± 0.041

4.187 ± 0.024

7.556 ± 0.037

2.126 ± 0.018

6.651 ± 0.033

5.604 ± 0.042

10.039 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.028 ± 0.02

3.733 ± 0.025

4.136 ± 0.023

3.583 ± 0.023

5.232 ± 0.033

6.223 ± 0.027

5.119 ± 0.025

7.006 ± 0.034

1.248 ± 0.015

3.484 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski