Amino acid dipepetide frequency for Paenibacillus sp. DMB20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.826AlaAla: 8.826 ± 0.142
0.758AlaCys: 0.758 ± 0.026
4.336AlaAsp: 4.336 ± 0.065
5.752AlaGlu: 5.752 ± 0.08
3.39AlaPhe: 3.39 ± 0.051
6.976AlaGly: 6.976 ± 0.084
1.385AlaHis: 1.385 ± 0.03
4.949AlaIle: 4.949 ± 0.06
4.442AlaLys: 4.442 ± 0.059
8.265AlaLeu: 8.265 ± 0.091
2.362AlaMet: 2.362 ± 0.039
2.485AlaAsn: 2.485 ± 0.041
2.571AlaPro: 2.571 ± 0.05
2.436AlaGln: 2.436 ± 0.046
3.528AlaArg: 3.528 ± 0.071
4.847AlaSer: 4.847 ± 0.066
3.015AlaThr: 3.015 ± 0.05
6.852AlaVal: 6.852 ± 0.085
0.95AlaTrp: 0.95 ± 0.029
2.622AlaTyr: 2.622 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
0.555CysAla: 0.555 ± 0.023
0.115CysCys: 0.115 ± 0.01
0.43CysAsp: 0.43 ± 0.019
0.473CysGlu: 0.473 ± 0.02
0.346CysPhe: 0.346 ± 0.017
0.862CysGly: 0.862 ± 0.029
0.207CysHis: 0.207 ± 0.014
0.519CysIle: 0.519 ± 0.022
0.349CysLys: 0.349 ± 0.015
0.816CysLeu: 0.816 ± 0.026
0.23CysMet: 0.23 ± 0.012
0.28CysAsn: 0.28 ± 0.017
0.403CysPro: 0.403 ± 0.02
0.224CysGln: 0.224 ± 0.013
0.549CysArg: 0.549 ± 0.022
0.587CysSer: 0.587 ± 0.026
0.408CysThr: 0.408 ± 0.019
0.463CysVal: 0.463 ± 0.018
0.095CysTrp: 0.095 ± 0.008
0.264CysTyr: 0.264 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
3.788AspAla: 3.788 ± 0.055
0.412AspCys: 0.412 ± 0.016
2.469AspAsp: 2.469 ± 0.046
3.886AspGlu: 3.886 ± 0.061
2.206AspPhe: 2.206 ± 0.041
4.007AspGly: 4.007 ± 0.06
1.237AspHis: 1.237 ± 0.037
3.75AspIle: 3.75 ± 0.052
2.865AspLys: 2.865 ± 0.051
4.951AspLeu: 4.951 ± 0.061
1.555AspMet: 1.555 ± 0.033
1.809AspAsn: 1.809 ± 0.039
2.537AspPro: 2.537 ± 0.047
1.862AspGln: 1.862 ± 0.038
2.953AspArg: 2.953 ± 0.045
2.822AspSer: 2.822 ± 0.046
2.379AspThr: 2.379 ± 0.038
3.499AspVal: 3.499 ± 0.059
0.828AspTrp: 0.828 ± 0.029
2.055AspTyr: 2.055 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
6.148GluAla: 6.148 ± 0.085
0.477GluCys: 0.477 ± 0.02
3.367GluAsp: 3.367 ± 0.048
5.702GluGlu: 5.702 ± 0.084
2.292GluPhe: 2.292 ± 0.041
4.714GluGly: 4.714 ± 0.066
1.544GluHis: 1.544 ± 0.034
4.447GluIle: 4.447 ± 0.07
4.291GluLys: 4.291 ± 0.058
7.072GluLeu: 7.072 ± 0.086
2.169GluMet: 2.169 ± 0.044
2.607GluAsn: 2.607 ± 0.047
2.447GluPro: 2.447 ± 0.041
3.448GluGln: 3.448 ± 0.056
4.188GluArg: 4.188 ± 0.071
3.677GluSer: 3.677 ± 0.052
3.487GluThr: 3.487 ± 0.057
4.484GluVal: 4.484 ± 0.062
0.959GluTrp: 0.959 ± 0.027
2.144GluTyr: 2.144 ± 0.036
0.0GluXaa: 0.0 ± 0.0
Phe
3.263PheAla: 3.263 ± 0.052
0.381PheCys: 0.381 ± 0.015
2.329PheAsp: 2.329 ± 0.044
2.54PheGlu: 2.54 ± 0.047
1.823PhePhe: 1.823 ± 0.042
3.276PheGly: 3.276 ± 0.052
0.943PheHis: 0.943 ± 0.029
2.972PheIle: 2.972 ± 0.058
2.092PheLys: 2.092 ± 0.036
3.923PheLeu: 3.923 ± 0.067
1.339PheMet: 1.339 ± 0.034
1.602PheAsn: 1.602 ± 0.041
1.635PhePro: 1.635 ± 0.036
1.435PheGln: 1.435 ± 0.032
2.158PheArg: 2.158 ± 0.039
2.835PheSer: 2.835 ± 0.046
2.405PheThr: 2.405 ± 0.048
2.896PheVal: 2.896 ± 0.049
0.539PheTrp: 0.539 ± 0.019
1.481PheTyr: 1.481 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
5.514GlyAla: 5.514 ± 0.079
0.744GlyCys: 0.744 ± 0.026
3.591GlyAsp: 3.591 ± 0.052
4.723GlyGlu: 4.723 ± 0.063
3.4GlyPhe: 3.4 ± 0.054
5.701GlyGly: 5.701 ± 0.084
1.512GlyHis: 1.512 ± 0.034
5.859GlyIle: 5.859 ± 0.069
4.745GlyLys: 4.745 ± 0.066
7.518GlyLeu: 7.518 ± 0.096
2.606GlyMet: 2.606 ± 0.04
2.749GlyAsn: 2.749 ± 0.06
2.025GlyPro: 2.025 ± 0.039
2.57GlyGln: 2.57 ± 0.051
3.804GlyArg: 3.804 ± 0.055
4.862GlySer: 4.862 ± 0.066
4.443GlyThr: 4.443 ± 0.062
5.147GlyVal: 5.147 ± 0.065
1.05GlyTrp: 1.05 ± 0.028
2.999GlyTyr: 2.999 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
1.629HisAla: 1.629 ± 0.041
0.209HisCys: 0.209 ± 0.013
1.077HisAsp: 1.077 ± 0.033
1.365HisGlu: 1.365 ± 0.034
1.027HisPhe: 1.027 ± 0.029
1.65HisGly: 1.65 ± 0.039
0.563HisHis: 0.563 ± 0.026
1.437HisIle: 1.437 ± 0.042
0.878HisLys: 0.878 ± 0.028
2.16HisLeu: 2.16 ± 0.042
0.637HisMet: 0.637 ± 0.019
0.69HisAsn: 0.69 ± 0.024
1.28HisPro: 1.28 ± 0.035
0.765HisGln: 0.765 ± 0.024
1.16HisArg: 1.16 ± 0.029
1.164HisSer: 1.164 ± 0.031
0.963HisThr: 0.963 ± 0.028
1.452HisVal: 1.452 ± 0.031
0.291HisTrp: 0.291 ± 0.013
0.86HisTyr: 0.86 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.676IleAla: 5.676 ± 0.073
0.611IleCys: 0.611 ± 0.021
3.562IleAsp: 3.562 ± 0.053
4.297IleGlu: 4.297 ± 0.069
2.472IlePhe: 2.472 ± 0.051
5.315IleGly: 5.315 ± 0.073
1.586IleHis: 1.586 ± 0.033
4.212IleIle: 4.212 ± 0.081
3.112IleLys: 3.112 ± 0.049
6.031IleLeu: 6.031 ± 0.082
1.81IleMet: 1.81 ± 0.044
2.246IleAsn: 2.246 ± 0.039
3.265IlePro: 3.265 ± 0.056
2.488IleGln: 2.488 ± 0.045
3.945IleArg: 3.945 ± 0.054
4.479IleSer: 4.479 ± 0.066
3.67IleThr: 3.67 ± 0.059
5.0IleVal: 5.0 ± 0.066
0.665IleTrp: 0.665 ± 0.024
2.067IleTyr: 2.067 ± 0.039
0.0IleXaa: 0.0 ± 0.0
Lys
4.448LysAla: 4.448 ± 0.061
0.302LysCys: 0.302 ± 0.017
3.125LysAsp: 3.125 ± 0.051
4.715LysGlu: 4.715 ± 0.067
1.609LysPhe: 1.609 ± 0.031
3.933LysGly: 3.933 ± 0.064
1.242LysHis: 1.242 ± 0.03
3.28LysIle: 3.28 ± 0.051
3.529LysLys: 3.529 ± 0.058
5.486LysLeu: 5.486 ± 0.063
1.722LysMet: 1.722 ± 0.033
2.256LysAsn: 2.256 ± 0.044
2.506LysPro: 2.506 ± 0.043
2.391LysGln: 2.391 ± 0.048
3.08LysArg: 3.08 ± 0.05
3.124LysSer: 3.124 ± 0.053
3.022LysThr: 3.022 ± 0.049
3.639LysVal: 3.639 ± 0.056
0.728LysTrp: 0.728 ± 0.026
1.903LysTyr: 1.903 ± 0.048
0.0LysXaa: 0.0 ± 0.0
Leu
8.108LeuAla: 8.108 ± 0.099
0.884LeuCys: 0.884 ± 0.029
5.158LeuAsp: 5.158 ± 0.061
6.433LeuGlu: 6.433 ± 0.079
4.613LeuPhe: 4.613 ± 0.076
6.873LeuGly: 6.873 ± 0.091
2.105LeuHis: 2.105 ± 0.04
6.355LeuIle: 6.355 ± 0.08
5.8LeuLys: 5.8 ± 0.066
10.838LeuLeu: 10.838 ± 0.15
2.831LeuMet: 2.831 ± 0.049
4.011LeuAsn: 4.011 ± 0.061
4.529LeuPro: 4.529 ± 0.067
3.844LeuGln: 3.844 ± 0.056
5.002LeuArg: 5.002 ± 0.079
7.064LeuSer: 7.064 ± 0.073
5.477LeuThr: 5.477 ± 0.056
6.166LeuVal: 6.166 ± 0.074
1.041LeuTrp: 1.041 ± 0.028
3.251LeuTyr: 3.251 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
2.424MetAla: 2.424 ± 0.046
0.17MetCys: 0.17 ± 0.013
1.705MetAsp: 1.705 ± 0.034
2.145MetGlu: 2.145 ± 0.042
1.104MetPhe: 1.104 ± 0.032
1.92MetGly: 1.92 ± 0.042
0.479MetHis: 0.479 ± 0.02
2.183MetIle: 2.183 ± 0.046
2.272MetLys: 2.272 ± 0.042
3.144MetLeu: 3.144 ± 0.048
0.976MetMet: 0.976 ± 0.029
1.676MetAsn: 1.676 ± 0.034
1.216MetPro: 1.216 ± 0.027
0.976MetGln: 0.976 ± 0.025
1.358MetArg: 1.358 ± 0.032
1.865MetSer: 1.865 ± 0.036
1.736MetThr: 1.736 ± 0.038
1.921MetVal: 1.921 ± 0.035
0.228MetTrp: 0.228 ± 0.013
0.783MetTyr: 0.783 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
2.784AsnAla: 2.784 ± 0.052
0.28AsnCys: 0.28 ± 0.016
1.901AsnAsp: 1.901 ± 0.04
2.591AsnGlu: 2.591 ± 0.048
1.345AsnPhe: 1.345 ± 0.034
3.279AsnGly: 3.279 ± 0.066
0.873AsnHis: 0.873 ± 0.027
2.484AsnIle: 2.484 ± 0.043
2.046AsnLys: 2.046 ± 0.048
3.269AsnLeu: 3.269 ± 0.05
1.134AsnMet: 1.134 ± 0.031
1.47AsnAsn: 1.47 ± 0.045
2.076AsnPro: 2.076 ± 0.046
1.33AsnGln: 1.33 ± 0.033
2.242AsnArg: 2.242 ± 0.036
1.971AsnSer: 1.971 ± 0.04
1.85AsnThr: 1.85 ± 0.04
2.601AsnVal: 2.601 ± 0.049
0.502AsnTrp: 0.502 ± 0.018
1.303AsnTyr: 1.303 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
3.365ProAla: 3.365 ± 0.056
0.266ProCys: 0.266 ± 0.017
2.828ProAsp: 2.828 ± 0.054
3.788ProGlu: 3.788 ± 0.052
1.894ProPhe: 1.894 ± 0.045
3.262ProGly: 3.262 ± 0.057
0.855ProHis: 0.855 ± 0.022
2.274ProIle: 2.274 ± 0.041
2.02ProLys: 2.02 ± 0.042
4.083ProLeu: 4.083 ± 0.062
1.068ProMet: 1.068 ± 0.025
1.435ProAsn: 1.435 ± 0.034
1.387ProPro: 1.387 ± 0.037
1.32ProGln: 1.32 ± 0.033
1.534ProArg: 1.534 ± 0.033
2.628ProSer: 2.628 ± 0.044
1.744ProThr: 1.744 ± 0.037
3.512ProVal: 3.512 ± 0.049
0.535ProTrp: 0.535 ± 0.024
1.564ProTyr: 1.564 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
3.225GlnAla: 3.225 ± 0.054
0.212GlnCys: 0.212 ± 0.013
1.674GlnAsp: 1.674 ± 0.038
2.592GlnGlu: 2.592 ± 0.045
1.386GlnPhe: 1.386 ± 0.032
2.625GlnGly: 2.625 ± 0.048
0.701GlnHis: 0.701 ± 0.025
2.298GlnIle: 2.298 ± 0.044
2.058GlnLys: 2.058 ± 0.038
3.772GlnLeu: 3.772 ± 0.057
1.135GlnMet: 1.135 ± 0.031
1.339GlnAsn: 1.339 ± 0.034
1.463GlnPro: 1.463 ± 0.032
1.512GlnGln: 1.512 ± 0.035
1.775GlnArg: 1.775 ± 0.038
2.172GlnSer: 2.172 ± 0.042
1.761GlnThr: 1.761 ± 0.035
2.358GlnVal: 2.358 ± 0.049
0.482GlnTrp: 0.482 ± 0.017
1.288GlnTyr: 1.288 ± 0.029
0.001GlnXaa: 0.001 ± 0.001
Arg
3.286ArgAla: 3.286 ± 0.057
0.398ArgCys: 0.398 ± 0.018
2.585ArgAsp: 2.585 ± 0.044
4.131ArgGlu: 4.131 ± 0.064
2.32ArgPhe: 2.32 ± 0.041
3.066ArgGly: 3.066 ± 0.052
1.259ArgHis: 1.259 ± 0.033
3.827ArgIle: 3.827 ± 0.053
3.394ArgLys: 3.394 ± 0.055
5.617ArgLeu: 5.617 ± 0.077
1.854ArgMet: 1.854 ± 0.035
2.101ArgAsn: 2.101 ± 0.043
1.787ArgPro: 1.787 ± 0.034
2.035ArgGln: 2.035 ± 0.04
2.968ArgArg: 2.968 ± 0.056
3.146ArgSer: 3.146 ± 0.05
2.686ArgThr: 2.686 ± 0.044
3.194ArgVal: 3.194 ± 0.054
0.665ArgTrp: 0.665 ± 0.024
2.0ArgTyr: 2.0 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
4.845SerAla: 4.845 ± 0.071
0.478SerCys: 0.478 ± 0.022
3.026SerAsp: 3.026 ± 0.05
3.678SerGlu: 3.678 ± 0.051
3.007SerPhe: 3.007 ± 0.047
5.63SerGly: 5.63 ± 0.08
1.272SerHis: 1.272 ± 0.037
4.168SerIle: 4.168 ± 0.054
3.221SerLys: 3.221 ± 0.058
6.425SerLeu: 6.425 ± 0.079
1.874SerMet: 1.874 ± 0.04
2.095SerAsn: 2.095 ± 0.047
2.593SerPro: 2.593 ± 0.047
1.836SerGln: 1.836 ± 0.038
3.39SerArg: 3.39 ± 0.054
4.168SerSer: 4.168 ± 0.075
2.902SerThr: 2.902 ± 0.052
4.564SerVal: 4.564 ± 0.055
0.833SerTrp: 0.833 ± 0.027
2.225SerTyr: 2.225 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
4.606ThrAla: 4.606 ± 0.063
0.372ThrCys: 0.372 ± 0.017
2.655ThrAsp: 2.655 ± 0.046
3.305ThrGlu: 3.305 ± 0.054
2.319ThrPhe: 2.319 ± 0.043
4.55ThrGly: 4.55 ± 0.06
0.996ThrHis: 0.996 ± 0.029
3.411ThrIle: 3.411 ± 0.057
2.362ThrLys: 2.362 ± 0.048
5.121ThrLeu: 5.121 ± 0.07
1.39ThrMet: 1.39 ± 0.031
1.663ThrAsn: 1.663 ± 0.04
2.473ThrPro: 2.473 ± 0.044
1.377ThrGln: 1.377 ± 0.034
2.191ThrArg: 2.191 ± 0.036
3.059ThrSer: 3.059 ± 0.056
2.508ThrThr: 2.508 ± 0.051
4.429ThrVal: 4.429 ± 0.065
0.597ThrTrp: 0.597 ± 0.022
1.803ThrTyr: 1.803 ± 0.04
0.001ThrXaa: 0.001 ± 0.001
Val
4.773ValAla: 4.773 ± 0.061
0.659ValCys: 0.659 ± 0.022
3.535ValAsp: 3.535 ± 0.055
4.443ValGlu: 4.443 ± 0.068
3.085ValPhe: 3.085 ± 0.05
4.334ValGly: 4.334 ± 0.055
1.486ValHis: 1.486 ± 0.033
4.997ValIle: 4.997 ± 0.07
4.103ValLys: 4.103 ± 0.058
7.227ValLeu: 7.227 ± 0.083
2.148ValMet: 2.148 ± 0.037
2.776ValAsn: 2.776 ± 0.051
3.183ValPro: 3.183 ± 0.045
2.424ValGln: 2.424 ± 0.044
3.639ValArg: 3.639 ± 0.054
4.889ValSer: 4.889 ± 0.059
4.277ValThr: 4.277 ± 0.061
4.763ValVal: 4.763 ± 0.065
0.867ValTrp: 0.867 ± 0.031
2.448ValTyr: 2.448 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
0.777TrpAla: 0.777 ± 0.023
0.099TrpCys: 0.099 ± 0.008
0.667TrpAsp: 0.667 ± 0.025
0.796TrpGlu: 0.796 ± 0.024
0.602TrpPhe: 0.602 ± 0.025
0.807TrpGly: 0.807 ± 0.026
0.232TrpHis: 0.232 ± 0.013
0.94TrpIle: 0.94 ± 0.029
0.774TrpLys: 0.774 ± 0.025
1.384TrpLeu: 1.384 ± 0.034
0.444TrpMet: 0.444 ± 0.019
0.717TrpAsn: 0.717 ± 0.024
0.372TrpPro: 0.372 ± 0.019
0.379TrpGln: 0.379 ± 0.018
0.62TrpArg: 0.62 ± 0.02
0.824TrpSer: 0.824 ± 0.028
0.701TrpThr: 0.701 ± 0.021
0.747TrpVal: 0.747 ± 0.023
0.167TrpTrp: 0.167 ± 0.012
0.431TrpTyr: 0.431 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.706TyrAla: 2.706 ± 0.05
0.346TyrCys: 0.346 ± 0.018
1.85TyrAsp: 1.85 ± 0.036
2.312TyrGlu: 2.312 ± 0.044
1.628TyrPhe: 1.628 ± 0.041
2.781TyrGly: 2.781 ± 0.041
0.78TyrHis: 0.78 ± 0.023
2.149TyrIle: 2.149 ± 0.038
1.689TyrLys: 1.689 ± 0.04
3.284TyrLeu: 3.284 ± 0.055
0.994TyrMet: 0.994 ± 0.029
1.323TyrAsn: 1.323 ± 0.033
1.614TyrPro: 1.614 ± 0.038
1.176TyrGln: 1.176 ± 0.031
2.239TyrArg: 2.239 ± 0.042
2.045TyrSer: 2.045 ± 0.04
1.75TyrThr: 1.75 ± 0.034
2.368TyrVal: 2.368 ± 0.043
0.46TyrTrp: 0.46 ± 0.02
1.351TyrTyr: 1.351 ± 0.034
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.001XaaMet: 0.001 ± 0.001
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.001XaaGln: 0.001 ± 0.001
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.001XaaXaa: 0.001 ± 0.002
Statistics based on 4674 proteins (1355747 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski