Pelagicola litorisediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Pelagicola

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3904 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y5SFA6|A0A1Y5SFA6_9RHOB Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B OS=Pelagicola litorisediminis OX=1312363 GN=gatB PE=3 SV=1
MM1 pKa = 7.69KK2 pKa = 10.19KK3 pKa = 9.92QLLSTSAIALGIAMAAPASAQDD25 pKa = 3.38WNMDD29 pKa = 2.55WGGYY33 pKa = 6.97QRR35 pKa = 11.84AGVGYY40 pKa = 10.03ADD42 pKa = 3.89VSGTLATGMDD52 pKa = 4.31LDD54 pKa = 5.51GIDD57 pKa = 3.58VFQSGEE63 pKa = 3.71IHH65 pKa = 5.92FTPSVTLDD73 pKa = 2.91NGLTFGVNIQLEE85 pKa = 4.8GATSGDD91 pKa = 4.3TIDD94 pKa = 3.64EE95 pKa = 4.49TYY97 pKa = 9.36ITISGDD103 pKa = 3.27NLGTITIGSEE113 pKa = 3.55NSEE116 pKa = 4.48GYY118 pKa = 10.25RR119 pKa = 11.84MSVGAPEE126 pKa = 4.08ATLMFINSPSVSAFIPFSAALTAGNQFRR154 pKa = 11.84TAMISTYY161 pKa = 9.99TEE163 pKa = 3.62VGGNNDD169 pKa = 3.13SQRR172 pKa = 11.84ISYY175 pKa = 7.68RR176 pKa = 11.84TPSFNGFQVGVSYY189 pKa = 11.2ARR191 pKa = 11.84DD192 pKa = 3.38TSQEE196 pKa = 3.88FSSGIDD202 pKa = 3.18TNAAGVLSDD211 pKa = 3.51IFDD214 pKa = 3.58VAVAYY219 pKa = 10.23SATVGTADD227 pKa = 3.2VALSARR233 pKa = 11.84WGTGDD238 pKa = 3.26VAGAAPVGGTAEE250 pKa = 4.14TWGLGASVGFNGITIGGSYY269 pKa = 10.72GEE271 pKa = 4.43NDD273 pKa = 3.37NPGTVNDD280 pKa = 3.66VNGWSLGATFDD291 pKa = 5.25APGPWTFGLSTYY303 pKa = 10.12QGEE306 pKa = 4.28SDD308 pKa = 3.56APGSQEE314 pKa = 4.33EE315 pKa = 4.4YY316 pKa = 9.48TAYY319 pKa = 10.1KK320 pKa = 10.37LGASRR325 pKa = 11.84SLGTGVSWDD334 pKa = 3.25IYY336 pKa = 10.45VVDD339 pKa = 4.38VEE341 pKa = 4.71AKK343 pKa = 8.09NTAGADD349 pKa = 3.42VDD351 pKa = 4.05GTAVGTTINLSFF363 pKa = 4.49

Molecular weight: 37.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y5RD45|A0A1Y5RD45_9RHOB Protein RecA OS=Pelagicola litorisediminis OX=1312363 GN=recA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.43EE41 pKa = 3.72LSAA44 pKa = 5.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3904

0

3904

1190632

29

1808

305.0

33.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.403 ± 0.049

0.985 ± 0.014

6.011 ± 0.033

5.78 ± 0.038

3.855 ± 0.025

8.522 ± 0.038

2.15 ± 0.02

5.478 ± 0.026

3.662 ± 0.03

9.982 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.864 ± 0.019

2.941 ± 0.023

4.925 ± 0.026

3.347 ± 0.022

6.378 ± 0.038

5.312 ± 0.028

5.484 ± 0.025

7.171 ± 0.033

1.421 ± 0.016

2.33 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski