Pepper cryptic virus 1
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F8VBM8|F8VBM8_9VIRU Coat protein OS=Pepper cryptic virus 1 OX=369643 PE=4 SV=1
MM1 pKa = 7.88 GDD3 pKa = 3.38 RR4 pKa = 11.84 VNAQDD9 pKa = 4.59 DD10 pKa = 4.3 DD11 pKa = 4.54 TVVPHH16 pKa = 6.01 QAPLQPAALQQDD28 pKa = 4.4 LTRR31 pKa = 11.84 SADD34 pKa = 3.58 YY35 pKa = 11.27 LLDD38 pKa = 3.36 NVRR41 pKa = 11.84 IGNHH45 pKa = 4.19 RR46 pKa = 11.84 QRR48 pKa = 11.84 YY49 pKa = 7.93 DD50 pKa = 2.67 KK51 pKa = 10.73 YY52 pKa = 10.4 RR53 pKa = 11.84 RR54 pKa = 11.84 YY55 pKa = 10.27 VLLRR59 pKa = 11.84 SSEE62 pKa = 4.01 IFTSLVAIYY71 pKa = 10.47 AHH73 pKa = 6.84 IFSSYY78 pKa = 6.8 WQHH81 pKa = 5.67 FRR83 pKa = 11.84 RR84 pKa = 11.84 FTDD87 pKa = 3.17 QFQAPTGVQLPTFVARR103 pKa = 11.84 VYY105 pKa = 10.69 ISTWLHH111 pKa = 6.62 DD112 pKa = 4.76 LYY114 pKa = 11.32 CSIRR118 pKa = 11.84 EE119 pKa = 4.26 ATRR122 pKa = 11.84 SISPLAFNEE131 pKa = 4.36 RR132 pKa = 11.84 YY133 pKa = 9.91 SYY135 pKa = 11.27 EE136 pKa = 3.94 LLPYY140 pKa = 8.73 STEE143 pKa = 3.83 YY144 pKa = 11.39 DD145 pKa = 3.39 PFLAFLSMSIKK156 pKa = 8.74 PTHH159 pKa = 6.11 IQHH162 pKa = 6.04 TPEE165 pKa = 3.65 NTLWIPILCEE175 pKa = 3.72 NYY177 pKa = 10.18 DD178 pKa = 3.24 WDD180 pKa = 4.94 RR181 pKa = 11.84 NEE183 pKa = 5.04 ANHH186 pKa = 6.33 NPFGITNFTLNSNLFYY202 pKa = 11.28 GLLAILKK209 pKa = 8.72 EE210 pKa = 3.99 RR211 pKa = 11.84 KK212 pKa = 8.52 EE213 pKa = 4.38 FKK215 pKa = 10.78 LSTLTTNTIGRR226 pKa = 11.84 PCWLFDD232 pKa = 3.0 WHH234 pKa = 7.77 DD235 pKa = 3.96 NVQVCAWFPRR245 pKa = 11.84 EE246 pKa = 4.33 ANFNSQDD253 pKa = 2.95 VTAAYY258 pKa = 9.49 IIGVACTPKK267 pKa = 10.53 LGPSDD272 pKa = 3.96 DD273 pKa = 5.02 DD274 pKa = 3.06 AWKK277 pKa = 10.77 YY278 pKa = 9.26 YY279 pKa = 11.16 ASLNSVPTFTPTEE292 pKa = 3.72 PRR294 pKa = 11.84 LTNRR298 pKa = 11.84 RR299 pKa = 11.84 SYY301 pKa = 10.72 GAYY304 pKa = 9.19 EE305 pKa = 3.77 VRR307 pKa = 11.84 TRR309 pKa = 11.84 EE310 pKa = 3.9 TEE312 pKa = 3.83 NNYY315 pKa = 9.68 FLPDD319 pKa = 3.29 SLLNIIEE326 pKa = 5.08 DD327 pKa = 4.21 FTATGTTQRR336 pKa = 11.84 RR337 pKa = 11.84 KK338 pKa = 9.5 IRR340 pKa = 11.84 RR341 pKa = 11.84 PSATSASTGAAIIIRR356 pKa = 11.84 DD357 pKa = 3.97 TPGTASTATTSTTEE371 pKa = 4.15 TEE373 pKa = 4.34 VTFPPVIRR381 pKa = 11.84 TKK383 pKa = 10.39 IRR385 pKa = 11.84 DD386 pKa = 3.28 WYY388 pKa = 7.78 YY389 pKa = 8.68 HH390 pKa = 5.59 SRR392 pKa = 11.84 VILEE396 pKa = 4.6 LEE398 pKa = 4.5 DD399 pKa = 3.79 NSRR402 pKa = 11.84 TAALRR407 pKa = 11.84 MFIIAA412 pKa = 4.64
Molecular weight: 47.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.156
IPC2_protein 6.148
IPC_protein 6.275
Toseland 6.576
ProMoST 6.664
Dawson 6.605
Bjellqvist 6.561
Wikipedia 6.62
Rodwell 6.59
Grimsley 6.722
Solomon 6.605
Lehninger 6.605
Nozaki 6.868
DTASelect 7.044
Thurlkill 7.073
EMBOSS 7.059
Sillero 6.985
Patrickios 3.948
IPC_peptide 6.634
IPC2_peptide 6.898
IPC2.peptide.svr19 6.798
Protein with the highest isoelectric point:
>tr|F8VBM8|F8VBM8_9VIRU Coat protein OS=Pepper cryptic virus 1 OX=369643 PE=4 SV=1
MM1 pKa = 7.47 VRR3 pKa = 11.84 GTLVGYY9 pKa = 10.32 DD10 pKa = 3.38 YY11 pKa = 11.01 TQFQGDD17 pKa = 4.27 LVKK20 pKa = 10.09 STHH23 pKa = 5.37 RR24 pKa = 11.84 HH25 pKa = 3.93 PHH27 pKa = 4.3 VVHH30 pKa = 6.91 RR31 pKa = 11.84 EE32 pKa = 3.47 IATTYY37 pKa = 8.82 VDD39 pKa = 3.4 QYY41 pKa = 11.03 AYY43 pKa = 10.35 EE44 pKa = 4.71 HH45 pKa = 6.97 IEE47 pKa = 4.42 TFSSLYY53 pKa = 10.1 PEE55 pKa = 6.06 LILKK59 pKa = 8.85 GWSRR63 pKa = 11.84 SYY65 pKa = 10.97 YY66 pKa = 10.59 LPEE69 pKa = 3.73 KK70 pKa = 10.48 HH71 pKa = 6.31 LAAVLNYY78 pKa = 10.89 SMPNVPASQLSQSLYY93 pKa = 9.15 RR94 pKa = 11.84 QAIEE98 pKa = 3.84 SAKK101 pKa = 10.78 NGFISLPRR109 pKa = 11.84 VKK111 pKa = 10.6 AFDD114 pKa = 3.61 VLTEE118 pKa = 3.89 MDD120 pKa = 3.63 QVPFKK125 pKa = 10.65 SSSSAGYY132 pKa = 10.35 NYY134 pKa = 9.84 TGRR137 pKa = 11.84 KK138 pKa = 8.81 GLIGDD143 pKa = 4.25 EE144 pKa = 4.11 NHH146 pKa = 6.1 SRR148 pKa = 11.84 AISIAKK154 pKa = 9.31 AVLWSAIKK162 pKa = 10.42 DD163 pKa = 3.46 DD164 pKa = 4.31 GEE166 pKa = 4.7 GIEE169 pKa = 4.28 HH170 pKa = 7.42 VIRR173 pKa = 11.84 TSVPDD178 pKa = 3.2 VGYY181 pKa = 8.74 TRR183 pKa = 11.84 TQLTDD188 pKa = 3.73 LLEE191 pKa = 4.36 KK192 pKa = 10.3 TKK194 pKa = 10.27 VRR196 pKa = 11.84 QVWGRR201 pKa = 11.84 AFHH204 pKa = 6.55 YY205 pKa = 10.25 ILLEE209 pKa = 3.96 GLVAYY214 pKa = 8.33 PFIQTVMSHH223 pKa = 5.15 KK224 pKa = 9.44 TFIHH228 pKa = 6.77 AGQDD232 pKa = 3.46 PLISVPRR239 pKa = 11.84 LLSDD243 pKa = 3.79 VALNCKK249 pKa = 9.3 WIYY252 pKa = 10.66 SLDD255 pKa = 3.26 WSQFDD260 pKa = 3.45 ATVSRR265 pKa = 11.84 FEE267 pKa = 3.43 IHH269 pKa = 6.71 AAFDD273 pKa = 3.98 IIKK276 pKa = 10.37 SYY278 pKa = 11.8 VDD280 pKa = 3.67 FPNYY284 pKa = 7.73 EE285 pKa = 3.86 TEE287 pKa = 3.97 QAFEE291 pKa = 3.83 ITRR294 pKa = 11.84 QLFIHH299 pKa = 6.53 KK300 pKa = 9.66 KK301 pKa = 8.28 VAVPDD306 pKa = 4.33 GYY308 pKa = 10.21 IYY310 pKa = 10.12 EE311 pKa = 4.24 SHH313 pKa = 6.77 KK314 pKa = 10.8 GIPSGSYY321 pKa = 7.44 YY322 pKa = 10.2 TSLVGSIINYY332 pKa = 9.38 LRR334 pKa = 11.84 INYY337 pKa = 8.45 LWRR340 pKa = 11.84 LLTGHH345 pKa = 7.76 PPQQCHH351 pKa = 5.2 TLGDD355 pKa = 3.97 DD356 pKa = 4.19 SLVGDD361 pKa = 4.06 NSYY364 pKa = 11.54 VNPQAIEE371 pKa = 3.66 EE372 pKa = 4.17 AANKK376 pKa = 9.98 LGWHH380 pKa = 6.56 FNPDD384 pKa = 2.74 KK385 pKa = 9.97 TQYY388 pKa = 9.19 STVPEE393 pKa = 4.7 EE394 pKa = 3.75 ITFLGRR400 pKa = 11.84 TYY402 pKa = 11.16 VGGLNKK408 pKa = 9.58 RR409 pKa = 11.84 DD410 pKa = 3.34 LTKK413 pKa = 10.69 CIRR416 pKa = 11.84 LLVYY420 pKa = 9.84 PEE422 pKa = 4.25 YY423 pKa = 9.79 PVEE426 pKa = 4.16 SGRR429 pKa = 11.84 ISAYY433 pKa = 8.84 RR434 pKa = 11.84 AKK436 pKa = 10.64 SIAQDD441 pKa = 3.16 AGGLSEE447 pKa = 4.34 VLNRR451 pKa = 11.84 IADD454 pKa = 3.76 KK455 pKa = 10.81 LRR457 pKa = 11.84 RR458 pKa = 11.84 IYY460 pKa = 9.59 GTASEE465 pKa = 4.82 EE466 pKa = 3.81 EE467 pKa = 3.97 VPIYY471 pKa = 9.99 FKK473 pKa = 10.73 RR474 pKa = 11.84 YY475 pKa = 8.22 VFGVV479 pKa = 3.32
Molecular weight: 54.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.533
IPC2_protein 6.517
IPC_protein 6.707
Toseland 7.029
ProMoST 7.088
Dawson 7.102
Bjellqvist 7.0
Wikipedia 7.117
Rodwell 7.088
Grimsley 7.205
Solomon 7.132
Lehninger 7.132
Nozaki 7.307
DTASelect 7.483
Thurlkill 7.512
EMBOSS 7.527
Sillero 7.468
Patrickios 3.808
IPC_peptide 7.146
IPC2_peptide 7.088
IPC2.peptide.svr19 6.987
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
891
412
479
445.5
51.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.071 ± 0.441
0.898 ± 0.2
5.387 ± 0.277
5.275 ± 0.266
4.377 ± 0.456
4.826 ± 1.212
3.03 ± 0.382
6.846 ± 0.185
3.704 ± 0.962
8.642 ± 0.093
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
0.786 ± 0.036
4.153 ± 0.752
5.163 ± 0.42
3.704 ± 0.194
6.622 ± 0.879
7.632 ± 0.376
7.856 ± 1.634
6.061 ± 1.071
1.796 ± 0.246
6.173 ± 0.528
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here