Pseudomonas phage PPSC2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Otagovirus; unclassified Otagovirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 168 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R2YAW9|A0A2R2YAW9_9CAUD Uncharacterized protein OS=Pseudomonas phage PPSC2 OX=2041350 GN=PPSC2_158 PE=4 SV=1
MM1 pKa = 7.39SVTIVSVYY9 pKa = 10.83LKK11 pKa = 9.74TEE13 pKa = 3.87SGDD16 pKa = 3.75GYY18 pKa = 10.97LFTYY22 pKa = 10.64DD23 pKa = 2.99RR24 pKa = 11.84VEE26 pKa = 4.17SVDD29 pKa = 3.5EE30 pKa = 4.22FVEE33 pKa = 4.84LVEE36 pKa = 5.55KK37 pKa = 11.05DD38 pKa = 3.59LGDD41 pKa = 3.63EE42 pKa = 3.92LAYY45 pKa = 10.71VYY47 pKa = 10.8SIDD50 pKa = 3.9LNAMYY55 pKa = 9.67TRR57 pKa = 11.84TEE59 pKa = 4.01TYY61 pKa = 9.69EE62 pKa = 3.98YY63 pKa = 11.3ADD65 pKa = 3.86ALQKK69 pKa = 10.56RR70 pKa = 11.84INEE73 pKa = 4.22MADD76 pKa = 3.34DD77 pKa = 4.0EE78 pKa = 4.56

Molecular weight: 9.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R2YAR3|A0A2R2YAR3_9CAUD Uncharacterized protein OS=Pseudomonas phage PPSC2 OX=2041350 GN=PPSC2_92 PE=4 SV=1
MM1 pKa = 7.23RR2 pKa = 11.84TVKK5 pKa = 10.5AQEE8 pKa = 3.85FGKK11 pKa = 10.23DD12 pKa = 3.53YY13 pKa = 10.97QFQAAKK19 pKa = 9.93RR20 pKa = 11.84KK21 pKa = 8.86ARR23 pKa = 11.84KK24 pKa = 9.52VEE26 pKa = 3.38IGRR29 pKa = 11.84RR30 pKa = 11.84SGRR33 pKa = 11.84RR34 pKa = 11.84GTPAWGNWNEE44 pKa = 3.85NDD46 pKa = 3.43

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

168

0

168

27681

30

785

164.8

18.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.468 ± 0.246

1.156 ± 0.084

6.001 ± 0.172

6.846 ± 0.226

3.927 ± 0.112

7.164 ± 0.177

1.976 ± 0.142

5.708 ± 0.139

6.311 ± 0.244

8.219 ± 0.185

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.045 ± 0.118

4.552 ± 0.202

3.587 ± 0.131

3.793 ± 0.121

4.548 ± 0.141

5.838 ± 0.191

6.391 ± 0.259

7.2 ± 0.189

1.546 ± 0.089

3.725 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski