Amino acid dipepetide frequency for Pseudomonas phage PPSC2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.02AlaAla: 8.02 ± 0.674
1.192AlaCys: 1.192 ± 0.263
4.516AlaAsp: 4.516 ± 0.459
5.491AlaGlu: 5.491 ± 0.434
3.396AlaPhe: 3.396 ± 0.287
5.166AlaGly: 5.166 ± 0.511
1.445AlaHis: 1.445 ± 0.228
5.202AlaIle: 5.202 ± 0.455
5.347AlaLys: 5.347 ± 0.485
7.876AlaLeu: 7.876 ± 0.586
2.71AlaMet: 2.71 ± 0.306
3.938AlaAsn: 3.938 ± 0.428
2.168AlaPro: 2.168 ± 0.256
3.504AlaGln: 3.504 ± 0.417
4.155AlaArg: 4.155 ± 0.416
4.805AlaSer: 4.805 ± 0.512
5.383AlaThr: 5.383 ± 0.671
6.178AlaVal: 6.178 ± 0.479
1.048AlaTrp: 1.048 ± 0.209
3.143AlaTyr: 3.143 ± 0.345
0.0AlaXaa: 0.0 ± 0.0
Cys
0.867CysAla: 0.867 ± 0.176
0.217CysCys: 0.217 ± 0.087
0.759CysAsp: 0.759 ± 0.202
0.903CysGlu: 0.903 ± 0.21
0.542CysPhe: 0.542 ± 0.123
0.65CysGly: 0.65 ± 0.175
0.578CysHis: 0.578 ± 0.143
0.47CysIle: 0.47 ± 0.129
0.614CysLys: 0.614 ± 0.174
0.614CysLeu: 0.614 ± 0.174
0.47CysMet: 0.47 ± 0.122
0.759CysAsn: 0.759 ± 0.146
0.578CysPro: 0.578 ± 0.153
0.506CysGln: 0.506 ± 0.131
0.434CysArg: 0.434 ± 0.113
0.686CysSer: 0.686 ± 0.2
0.47CysThr: 0.47 ± 0.118
0.939CysVal: 0.939 ± 0.199
0.108CysTrp: 0.108 ± 0.066
0.397CysTyr: 0.397 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
4.48AspAla: 4.48 ± 0.404
0.903AspCys: 0.903 ± 0.184
3.504AspAsp: 3.504 ± 0.358
3.793AspGlu: 3.793 ± 0.394
2.421AspPhe: 2.421 ± 0.302
5.455AspGly: 5.455 ± 0.451
1.12AspHis: 1.12 ± 0.206
3.251AspIle: 3.251 ± 0.328
4.155AspLys: 4.155 ± 0.358
5.13AspLeu: 5.13 ± 0.351
1.951AspMet: 1.951 ± 0.281
3.143AspAsn: 3.143 ± 0.341
2.276AspPro: 2.276 ± 0.287
1.879AspGln: 1.879 ± 0.247
2.276AspArg: 2.276 ± 0.259
3.757AspSer: 3.757 ± 0.346
3.035AspThr: 3.035 ± 0.294
4.191AspVal: 4.191 ± 0.353
1.084AspTrp: 1.084 ± 0.164
2.204AspTyr: 2.204 ± 0.309
0.0AspXaa: 0.0 ± 0.0
Glu
5.961GluAla: 5.961 ± 0.467
0.795GluCys: 0.795 ± 0.193
4.299GluAsp: 4.299 ± 0.436
5.13GluGlu: 5.13 ± 0.496
3.685GluPhe: 3.685 ± 0.349
3.902GluGly: 3.902 ± 0.369
1.228GluHis: 1.228 ± 0.215
3.504GluIle: 3.504 ± 0.376
3.938GluLys: 3.938 ± 0.46
7.081GluLeu: 7.081 ± 0.554
2.529GluMet: 2.529 ± 0.321
2.529GluAsn: 2.529 ± 0.279
1.337GluPro: 1.337 ± 0.224
2.962GluGln: 2.962 ± 0.284
3.324GluArg: 3.324 ± 0.376
3.577GluSer: 3.577 ± 0.361
4.082GluThr: 4.082 ± 0.325
4.841GluVal: 4.841 ± 0.412
1.337GluTrp: 1.337 ± 0.193
2.421GluTyr: 2.421 ± 0.264
0.0GluXaa: 0.0 ± 0.0
Phe
2.999PheAla: 2.999 ± 0.347
0.434PheCys: 0.434 ± 0.126
2.854PheAsp: 2.854 ± 0.334
3.468PheGlu: 3.468 ± 0.376
1.445PhePhe: 1.445 ± 0.217
2.926PheGly: 2.926 ± 0.377
0.578PheHis: 0.578 ± 0.144
1.842PheIle: 1.842 ± 0.27
2.421PheLys: 2.421 ± 0.286
2.962PheLeu: 2.962 ± 0.323
1.084PheMet: 1.084 ± 0.205
1.915PheAsn: 1.915 ± 0.287
1.698PhePro: 1.698 ± 0.239
1.445PheGln: 1.445 ± 0.209
1.698PheArg: 1.698 ± 0.253
2.782PheSer: 2.782 ± 0.291
2.565PheThr: 2.565 ± 0.278
2.421PheVal: 2.421 ± 0.31
0.434PheTrp: 0.434 ± 0.119
1.301PheTyr: 1.301 ± 0.224
0.0PheXaa: 0.0 ± 0.0
Gly
5.311GlyAla: 5.311 ± 0.631
1.084GlyCys: 1.084 ± 0.196
4.118GlyAsp: 4.118 ± 0.419
4.118GlyGlu: 4.118 ± 0.372
3.143GlyPhe: 3.143 ± 0.353
5.78GlyGly: 5.78 ± 0.52
1.553GlyHis: 1.553 ± 0.287
4.191GlyIle: 4.191 ± 0.379
4.624GlyLys: 4.624 ± 0.374
5.6GlyLeu: 5.6 ± 0.448
2.493GlyMet: 2.493 ± 0.287
3.36GlyAsn: 3.36 ± 0.406
1.481GlyPro: 1.481 ± 0.217
1.987GlyGln: 1.987 ± 0.209
2.926GlyArg: 2.926 ± 0.294
4.697GlySer: 4.697 ± 0.427
4.697GlyThr: 4.697 ± 0.46
5.419GlyVal: 5.419 ± 0.48
1.734GlyTrp: 1.734 ± 0.255
3.324GlyTyr: 3.324 ± 0.343
0.0GlyXaa: 0.0 ± 0.0
His
1.156HisAla: 1.156 ± 0.202
0.325HisCys: 0.325 ± 0.101
1.12HisAsp: 1.12 ± 0.208
1.12HisGlu: 1.12 ± 0.203
1.084HisPhe: 1.084 ± 0.223
1.626HisGly: 1.626 ± 0.247
0.47HisHis: 0.47 ± 0.145
1.12HisIle: 1.12 ± 0.195
1.301HisLys: 1.301 ± 0.2
1.373HisLeu: 1.373 ± 0.247
0.65HisMet: 0.65 ± 0.145
0.939HisAsn: 0.939 ± 0.234
0.795HisPro: 0.795 ± 0.184
0.47HisGln: 0.47 ± 0.137
1.12HisArg: 1.12 ± 0.198
1.156HisSer: 1.156 ± 0.203
1.445HisThr: 1.445 ± 0.252
0.975HisVal: 0.975 ± 0.176
0.397HisTrp: 0.397 ± 0.108
1.12HisTyr: 1.12 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
4.913IleAla: 4.913 ± 0.35
0.831IleCys: 0.831 ± 0.177
4.624IleAsp: 4.624 ± 0.386
4.082IleGlu: 4.082 ± 0.37
1.734IlePhe: 1.734 ± 0.231
4.046IleGly: 4.046 ± 0.38
0.975IleHis: 0.975 ± 0.19
3.071IleIle: 3.071 ± 0.303
3.504IleLys: 3.504 ± 0.321
4.335IleLeu: 4.335 ± 0.411
1.264IleMet: 1.264 ± 0.188
2.999IleAsn: 2.999 ± 0.305
2.565IlePro: 2.565 ± 0.307
2.168IleGln: 2.168 ± 0.227
2.746IleArg: 2.746 ± 0.334
2.999IleSer: 2.999 ± 0.355
3.649IleThr: 3.649 ± 0.399
4.118IleVal: 4.118 ± 0.425
0.759IleTrp: 0.759 ± 0.207
1.698IleTyr: 1.698 ± 0.226
0.0IleXaa: 0.0 ± 0.0
Lys
6.973LysAla: 6.973 ± 0.71
0.614LysCys: 0.614 ± 0.159
3.829LysAsp: 3.829 ± 0.355
4.877LysGlu: 4.877 ± 0.504
1.951LysPhe: 1.951 ± 0.283
4.48LysGly: 4.48 ± 0.516
0.867LysHis: 0.867 ± 0.19
3.974LysIle: 3.974 ± 0.377
2.673LysLys: 2.673 ± 0.299
5.094LysLeu: 5.094 ± 0.434
2.095LysMet: 2.095 ± 0.3
2.023LysAsn: 2.023 ± 0.232
2.204LysPro: 2.204 ± 0.335
2.89LysGln: 2.89 ± 0.308
3.504LysArg: 3.504 ± 0.419
3.107LysSer: 3.107 ± 0.416
3.251LysThr: 3.251 ± 0.387
5.202LysVal: 5.202 ± 0.519
0.867LysTrp: 0.867 ± 0.154
2.601LysTyr: 2.601 ± 0.287
0.0LysXaa: 0.0 ± 0.0
Leu
7.803LeuAla: 7.803 ± 0.557
0.506LeuCys: 0.506 ± 0.151
6.033LeuAsp: 6.033 ± 0.424
6.539LeuGlu: 6.539 ± 0.542
2.999LeuPhe: 2.999 ± 0.336
4.805LeuGly: 4.805 ± 0.55
1.373LeuHis: 1.373 ± 0.207
4.949LeuIle: 4.949 ± 0.412
5.816LeuLys: 5.816 ± 0.489
5.816LeuLeu: 5.816 ± 0.606
2.24LeuMet: 2.24 ± 0.278
3.829LeuAsn: 3.829 ± 0.401
3.215LeuPro: 3.215 ± 0.309
2.999LeuGln: 2.999 ± 0.359
3.468LeuArg: 3.468 ± 0.349
5.564LeuSer: 5.564 ± 0.385
6.105LeuThr: 6.105 ± 0.551
4.877LeuVal: 4.877 ± 0.423
0.903LeuTrp: 0.903 ± 0.188
2.348LeuTyr: 2.348 ± 0.329
0.0LeuXaa: 0.0 ± 0.0
Met
3.613MetAla: 3.613 ± 0.325
0.253MetCys: 0.253 ± 0.103
1.12MetAsp: 1.12 ± 0.213
1.698MetGlu: 1.698 ± 0.281
1.156MetPhe: 1.156 ± 0.191
1.77MetGly: 1.77 ± 0.265
0.614MetHis: 0.614 ± 0.162
2.132MetIle: 2.132 ± 0.289
2.384MetLys: 2.384 ± 0.293
2.529MetLeu: 2.529 ± 0.31
0.614MetMet: 0.614 ± 0.146
1.084MetAsn: 1.084 ± 0.22
1.048MetPro: 1.048 ± 0.221
1.012MetGln: 1.012 ± 0.207
1.264MetArg: 1.264 ± 0.207
2.601MetSer: 2.601 ± 0.255
2.348MetThr: 2.348 ± 0.293
1.553MetVal: 1.553 ± 0.268
0.506MetTrp: 0.506 ± 0.127
1.156MetTyr: 1.156 ± 0.188
0.0MetXaa: 0.0 ± 0.0
Asn
3.288AsnAla: 3.288 ± 0.361
0.361AsnCys: 0.361 ± 0.111
1.987AsnAsp: 1.987 ± 0.267
2.673AsnGlu: 2.673 ± 0.271
1.084AsnPhe: 1.084 ± 0.199
4.155AsnGly: 4.155 ± 0.464
1.084AsnHis: 1.084 ± 0.182
3.071AsnIle: 3.071 ± 0.436
3.107AsnLys: 3.107 ± 0.337
3.829AsnLeu: 3.829 ± 0.534
0.831AsnMet: 0.831 ± 0.164
2.601AsnAsn: 2.601 ± 0.391
2.493AsnPro: 2.493 ± 0.33
1.662AsnGln: 1.662 ± 0.251
1.951AsnArg: 1.951 ± 0.247
2.818AsnSer: 2.818 ± 0.299
2.673AsnThr: 2.673 ± 0.283
2.999AsnVal: 2.999 ± 0.327
0.867AsnTrp: 0.867 ± 0.179
1.987AsnTyr: 1.987 ± 0.303
0.0AsnXaa: 0.0 ± 0.0
Pro
2.854ProAla: 2.854 ± 0.337
0.434ProCys: 0.434 ± 0.12
1.987ProAsp: 1.987 ± 0.283
3.613ProGlu: 3.613 ± 0.413
1.445ProPhe: 1.445 ± 0.216
2.059ProGly: 2.059 ± 0.257
0.975ProHis: 0.975 ± 0.172
1.481ProIle: 1.481 ± 0.193
2.132ProLys: 2.132 ± 0.314
2.854ProLeu: 2.854 ± 0.286
1.228ProMet: 1.228 ± 0.187
1.445ProAsn: 1.445 ± 0.227
1.012ProPro: 1.012 ± 0.209
1.445ProGln: 1.445 ± 0.227
1.409ProArg: 1.409 ± 0.272
2.023ProSer: 2.023 ± 0.305
2.312ProThr: 2.312 ± 0.279
3.288ProVal: 3.288 ± 0.35
0.578ProTrp: 0.578 ± 0.156
1.301ProTyr: 1.301 ± 0.205
0.0ProXaa: 0.0 ± 0.0
Gln
3.793GlnAla: 3.793 ± 0.395
0.506GlnCys: 0.506 ± 0.137
1.662GlnAsp: 1.662 ± 0.268
2.348GlnGlu: 2.348 ± 0.319
1.77GlnPhe: 1.77 ± 0.236
2.529GlnGly: 2.529 ± 0.265
0.795GlnHis: 0.795 ± 0.183
2.095GlnIle: 2.095 ± 0.268
2.204GlnLys: 2.204 ± 0.299
3.577GlnLeu: 3.577 ± 0.394
1.156GlnMet: 1.156 ± 0.187
1.553GlnAsn: 1.553 ± 0.209
1.012GlnPro: 1.012 ± 0.247
1.987GlnGln: 1.987 ± 0.287
2.023GlnArg: 2.023 ± 0.291
1.734GlnSer: 1.734 ± 0.24
1.879GlnThr: 1.879 ± 0.26
3.107GlnVal: 3.107 ± 0.325
0.65GlnTrp: 0.65 ± 0.158
1.553GlnTyr: 1.553 ± 0.237
0.0GlnXaa: 0.0 ± 0.0
Arg
3.432ArgAla: 3.432 ± 0.365
0.289ArgCys: 0.289 ± 0.104
2.637ArgAsp: 2.637 ± 0.309
2.926ArgGlu: 2.926 ± 0.339
1.553ArgPhe: 1.553 ± 0.202
2.962ArgGly: 2.962 ± 0.372
1.084ArgHis: 1.084 ± 0.24
2.565ArgIle: 2.565 ± 0.285
3.071ArgLys: 3.071 ± 0.368
4.227ArgLeu: 4.227 ± 0.384
1.626ArgMet: 1.626 ± 0.2
2.204ArgAsn: 2.204 ± 0.251
1.445ArgPro: 1.445 ± 0.224
1.77ArgGln: 1.77 ± 0.24
2.312ArgArg: 2.312 ± 0.323
2.421ArgSer: 2.421 ± 0.288
2.962ArgThr: 2.962 ± 0.343
3.649ArgVal: 3.649 ± 0.305
0.795ArgTrp: 0.795 ± 0.175
1.553ArgTyr: 1.553 ± 0.195
0.0ArgXaa: 0.0 ± 0.0
Ser
4.408SerAla: 4.408 ± 0.577
0.831SerCys: 0.831 ± 0.186
3.36SerAsp: 3.36 ± 0.351
3.396SerGlu: 3.396 ± 0.363
2.276SerPhe: 2.276 ± 0.303
4.877SerGly: 4.877 ± 0.398
1.517SerHis: 1.517 ± 0.234
2.818SerIle: 2.818 ± 0.363
3.468SerLys: 3.468 ± 0.329
4.733SerLeu: 4.733 ± 0.412
1.59SerMet: 1.59 ± 0.273
2.565SerAsn: 2.565 ± 0.442
2.312SerPro: 2.312 ± 0.287
2.312SerGln: 2.312 ± 0.313
2.746SerArg: 2.746 ± 0.325
2.89SerSer: 2.89 ± 0.415
3.757SerThr: 3.757 ± 0.413
5.347SerVal: 5.347 ± 0.388
0.795SerTrp: 0.795 ± 0.155
2.384SerTyr: 2.384 ± 0.261
0.0SerXaa: 0.0 ± 0.0
Thr
5.311ThrAla: 5.311 ± 0.57
0.434ThrCys: 0.434 ± 0.124
3.613ThrAsp: 3.613 ± 0.359
3.721ThrGlu: 3.721 ± 0.394
2.565ThrPhe: 2.565 ± 0.308
5.564ThrGly: 5.564 ± 0.517
1.048ThrHis: 1.048 ± 0.226
4.082ThrIle: 4.082 ± 0.33
3.468ThrLys: 3.468 ± 0.327
5.311ThrLeu: 5.311 ± 0.454
2.059ThrMet: 2.059 ± 0.27
2.493ThrAsn: 2.493 ± 0.338
3.36ThrPro: 3.36 ± 0.351
2.782ThrGln: 2.782 ± 0.305
2.421ThrArg: 2.421 ± 0.302
3.757ThrSer: 3.757 ± 0.45
4.444ThrThr: 4.444 ± 0.729
4.227ThrVal: 4.227 ± 0.402
0.939ThrTrp: 0.939 ± 0.16
2.312ThrTyr: 2.312 ± 0.278
0.0ThrXaa: 0.0 ± 0.0
Val
5.672ValAla: 5.672 ± 0.478
0.939ValCys: 0.939 ± 0.221
4.516ValAsp: 4.516 ± 0.405
5.13ValGlu: 5.13 ± 0.496
2.999ValPhe: 2.999 ± 0.34
5.275ValGly: 5.275 ± 0.464
1.084ValHis: 1.084 ± 0.167
3.649ValIle: 3.649 ± 0.374
4.805ValLys: 4.805 ± 0.538
5.455ValLeu: 5.455 ± 0.477
2.601ValMet: 2.601 ± 0.256
2.89ValAsn: 2.89 ± 0.39
2.926ValPro: 2.926 ± 0.332
2.312ValGln: 2.312 ± 0.286
3.288ValArg: 3.288 ± 0.333
4.299ValSer: 4.299 ± 0.404
5.058ValThr: 5.058 ± 0.408
5.636ValVal: 5.636 ± 0.558
0.831ValTrp: 0.831 ± 0.167
2.637ValTyr: 2.637 ± 0.255
0.0ValXaa: 0.0 ± 0.0
Trp
1.156TrpAla: 1.156 ± 0.224
0.217TrpCys: 0.217 ± 0.083
1.264TrpAsp: 1.264 ± 0.191
1.228TrpGlu: 1.228 ± 0.221
0.723TrpPhe: 0.723 ± 0.144
0.831TrpGly: 0.831 ± 0.171
0.542TrpHis: 0.542 ± 0.129
1.264TrpIle: 1.264 ± 0.198
0.759TrpLys: 0.759 ± 0.14
1.806TrpLeu: 1.806 ± 0.211
0.253TrpMet: 0.253 ± 0.09
0.723TrpAsn: 0.723 ± 0.188
0.578TrpPro: 0.578 ± 0.139
0.434TrpGln: 0.434 ± 0.135
0.434TrpArg: 0.434 ± 0.141
0.506TrpSer: 0.506 ± 0.138
1.048TrpThr: 1.048 ± 0.177
0.867TrpVal: 0.867 ± 0.184
0.325TrpTrp: 0.325 ± 0.096
0.506TrpTyr: 0.506 ± 0.127
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.673TyrAla: 2.673 ± 0.271
0.397TyrCys: 0.397 ± 0.12
2.204TyrAsp: 2.204 ± 0.274
2.204TyrGlu: 2.204 ± 0.281
1.301TyrPhe: 1.301 ± 0.237
2.782TyrGly: 2.782 ± 0.314
0.831TyrHis: 0.831 ± 0.186
2.348TyrIle: 2.348 ± 0.258
3.324TyrLys: 3.324 ± 0.373
1.987TyrLeu: 1.987 ± 0.275
0.975TyrMet: 0.975 ± 0.166
2.529TyrAsn: 2.529 ± 0.289
1.373TyrPro: 1.373 ± 0.166
1.409TyrGln: 1.409 ± 0.23
1.987TyrArg: 1.987 ± 0.283
2.204TyrSer: 2.204 ± 0.252
2.746TyrThr: 2.746 ± 0.288
2.168TyrVal: 2.168 ± 0.251
0.506TyrTrp: 0.506 ± 0.124
1.301TyrTyr: 1.301 ± 0.216
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 168 proteins (27681 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski