Microbacterium rhizomatis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3790 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J5J898|A0A5J5J898_9MICO 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Microbacterium rhizomatis OX=1631477 GN=metE PE=3 SV=1
MM1 pKa = 7.56AALSGCTSAVSLQPAAAANDD21 pKa = 4.12PACADD26 pKa = 3.5ITVHH30 pKa = 6.64LPASVSGQDD39 pKa = 4.47RR40 pKa = 11.84RR41 pKa = 11.84WTDD44 pKa = 3.3AQATGAWGDD53 pKa = 3.64PASVILTCGITPPGPTTLPCTPVAGVDD80 pKa = 3.64WIIDD84 pKa = 3.52DD85 pKa = 3.96TDD87 pKa = 3.19APRR90 pKa = 11.84YY91 pKa = 9.48RR92 pKa = 11.84VTTFGRR98 pKa = 11.84VPAVQIYY105 pKa = 10.36LDD107 pKa = 3.71NDD109 pKa = 3.82VVSSADD115 pKa = 3.46VLDD118 pKa = 3.78TLSRR122 pKa = 11.84IVATLPTDD130 pKa = 4.23GSACTDD136 pKa = 3.61TASATASATPP146 pKa = 3.58

Molecular weight: 14.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J5J7R9|A0A5J5J7R9_9MICO Uncharacterized protein OS=Microbacterium rhizomatis OX=1631477 GN=F6B43_08130 PE=4 SV=1
MM1 pKa = 7.33VSGSRR6 pKa = 11.84IRR8 pKa = 11.84RR9 pKa = 11.84VMVSGSRR16 pKa = 11.84IRR18 pKa = 11.84RR19 pKa = 11.84VMVSGSRR26 pKa = 11.84IRR28 pKa = 11.84RR29 pKa = 11.84VMVSGSLIRR38 pKa = 11.84RR39 pKa = 11.84VMVSGSRR46 pKa = 11.84TPPVMVSGSRR56 pKa = 11.84TPPVMVSGSRR66 pKa = 11.84TPPVMVSGSRR76 pKa = 11.84TPPVMVSGSRR86 pKa = 11.84TPPVMVSGSRR96 pKa = 11.84TPPVMVSGSRR106 pKa = 11.84TPPVMVSGSRR116 pKa = 11.84IRR118 pKa = 11.84RR119 pKa = 11.84VMVTALTRR127 pKa = 11.84PARR130 pKa = 11.84AVIVQSARR138 pKa = 11.84ARR140 pKa = 11.84ICARR144 pKa = 11.84YY145 pKa = 8.76GRR147 pKa = 11.84AMTIRR152 pKa = 3.8

Molecular weight: 16.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3790

0

3790

1248223

26

2536

329.3

35.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.523 ± 0.054

0.513 ± 0.009

6.281 ± 0.035

5.315 ± 0.039

3.125 ± 0.022

8.993 ± 0.032

2.006 ± 0.02

4.787 ± 0.031

1.818 ± 0.023

9.776 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.803 ± 0.014

1.95 ± 0.02

5.514 ± 0.031

2.756 ± 0.02

7.315 ± 0.046

5.831 ± 0.028

6.252 ± 0.049

8.828 ± 0.034

1.559 ± 0.017

2.053 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski