Butyrivibrio virus Idris
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6H1MY30|A0A6H1MY30_9CAUD Uncharacterized protein OS=Butyrivibrio virus Idris OX=2696360 PE=4 SV=1
MM1 pKa = 7.5 PVWKK5 pKa = 9.88 YY6 pKa = 11.4 DD7 pKa = 3.24 EE8 pKa = 4.66 AAISRR13 pKa = 11.84 AEE15 pKa = 3.98 YY16 pKa = 9.96 EE17 pKa = 4.25 KK18 pKa = 11.54 NEE20 pKa = 4.45 AIYY23 pKa = 10.89 SEE25 pKa = 4.51 LVVMIEE31 pKa = 3.75 QQAANDD37 pKa = 3.86 SVYY40 pKa = 11.27 GDD42 pKa = 4.06 VLLQQADD49 pKa = 3.68 AQATLAEE56 pKa = 4.15 QDD58 pKa = 3.59 EE59 pKa = 4.63 VLADD63 pKa = 3.45 ILLTVQGVV71 pKa = 3.15
Molecular weight: 7.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.811
IPC2_protein 3.719
IPC_protein 3.605
Toseland 3.427
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.528
Rodwell 3.452
Grimsley 3.35
Solomon 3.554
Lehninger 3.503
Nozaki 3.732
DTASelect 3.859
Thurlkill 3.49
EMBOSS 3.541
Sillero 3.719
Patrickios 1.812
IPC_peptide 3.554
IPC2_peptide 3.694
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A6H1MY30|A0A6H1MY30_9CAUD Uncharacterized protein OS=Butyrivibrio virus Idris OX=2696360 PE=4 SV=1
MM1 pKa = 7.21 KK2 pKa = 9.67 TYY4 pKa = 10.8 KK5 pKa = 10.35 HH6 pKa = 6.32 LLEE9 pKa = 4.87 QIADD13 pKa = 3.53 KK14 pKa = 11.5 DD15 pKa = 3.98 NVRR18 pKa = 11.84 KK19 pKa = 10.12 AILNASKK26 pKa = 10.56 RR27 pKa = 11.84 KK28 pKa = 8.87 RR29 pKa = 11.84 HH30 pKa = 5.85 RR31 pKa = 11.84 KK32 pKa = 8.88 DD33 pKa = 3.02 VKK35 pKa = 10.61 QVIEE39 pKa = 4.49 NIDD42 pKa = 3.31 YY43 pKa = 10.63 HH44 pKa = 7.75 VEE46 pKa = 4.18 VVHH49 pKa = 6.78 NMLLAGSYY57 pKa = 9.03 KK58 pKa = 10.45 AHH60 pKa = 6.65 VDD62 pKa = 3.37 APCIVNEE69 pKa = 4.33 GTHH72 pKa = 5.63 HH73 pKa = 6.33 KK74 pKa = 10.07 VRR76 pKa = 11.84 RR77 pKa = 11.84 IRR79 pKa = 11.84 KK80 pKa = 7.5 PHH82 pKa = 5.59 YY83 pKa = 10.02 KK84 pKa = 9.19 YY85 pKa = 10.74 DD86 pKa = 3.9 QIIHH90 pKa = 6.03 HH91 pKa = 6.92 CIIQVLQPILTAPMYY106 pKa = 9.71 EE107 pKa = 4.24 YY108 pKa = 11.23 SCGSIPKK115 pKa = 10.12 RR116 pKa = 11.84 GAHH119 pKa = 4.87 YY120 pKa = 10.2 GKK122 pKa = 10.16 RR123 pKa = 11.84 RR124 pKa = 11.84 LEE126 pKa = 3.42 KK127 pKa = 10.05 WIRR130 pKa = 11.84 NDD132 pKa = 3.33 AKK134 pKa = 9.11 GTKK137 pKa = 9.62 YY138 pKa = 10.16 VFKK141 pKa = 10.39 MDD143 pKa = 2.78 IKK145 pKa = 10.73 HH146 pKa = 6.12 FYY148 pKa = 10.53 EE149 pKa = 5.45 SVDD152 pKa = 3.49 QDD154 pKa = 3.78 VLKK157 pKa = 11.38 SMLKK161 pKa = 10.64 AKK163 pKa = 10.32 IKK165 pKa = 10.16 DD166 pKa = 3.18 WRR168 pKa = 11.84 ALEE171 pKa = 4.68 LIYY174 pKa = 10.25 TVIDD178 pKa = 3.56 SCEE181 pKa = 3.85 KK182 pKa = 10.58 GLPLGNYY189 pKa = 6.78 TSQWFANFMLTPLDD203 pKa = 4.06 HH204 pKa = 6.69 YY205 pKa = 11.05 IKK207 pKa = 10.22 EE208 pKa = 4.13 QLHH211 pKa = 5.78 AKK213 pKa = 10.29 YY214 pKa = 10.8 YY215 pKa = 8.19 MRR217 pKa = 11.84 YY218 pKa = 8.23 MDD220 pKa = 6.36 DD221 pKa = 3.37 IVILGGNKK229 pKa = 9.47 KK230 pKa = 9.03 EE231 pKa = 3.61 LHH233 pKa = 6.72 KK234 pKa = 10.34 IHH236 pKa = 6.88 KK237 pKa = 10.24 AIEE240 pKa = 4.45 KK241 pKa = 9.06 YY242 pKa = 10.54 LSEE245 pKa = 4.22 RR246 pKa = 11.84 LHH248 pKa = 7.91 LRR250 pKa = 11.84 IKK252 pKa = 10.32 EE253 pKa = 3.76 NWQVFRR259 pKa = 11.84 LVYY262 pKa = 9.54 TDD264 pKa = 3.08 RR265 pKa = 11.84 NGRR268 pKa = 11.84 QRR270 pKa = 11.84 GRR272 pKa = 11.84 SLDD275 pKa = 3.5 FMGWQFYY282 pKa = 10.25 RR283 pKa = 11.84 EE284 pKa = 3.94 KK285 pKa = 10.33 TILRR289 pKa = 11.84 EE290 pKa = 4.11 SIYY293 pKa = 10.91 LRR295 pKa = 11.84 IVRR298 pKa = 11.84 KK299 pKa = 9.15 ARR301 pKa = 11.84 HH302 pKa = 5.31 VGKK305 pKa = 8.76 HH306 pKa = 4.3 TTIQGAQGMISYY318 pKa = 8.93 MGYY321 pKa = 9.7 IKK323 pKa = 9.8 HH324 pKa = 6.04 TDD326 pKa = 2.91 CYY328 pKa = 9.57 GTYY331 pKa = 9.5 RR332 pKa = 11.84 DD333 pKa = 3.92 YY334 pKa = 11.15 IRR336 pKa = 11.84 PYY338 pKa = 10.86 VNIGKK343 pKa = 9.6 LKK345 pKa = 10.57 KK346 pKa = 9.2 FTSKK350 pKa = 10.33 RR351 pKa = 11.84 AKK353 pKa = 10.42 KK354 pKa = 9.95 GVLKK358 pKa = 11.1 NGVEE362 pKa = 4.04 KK363 pKa = 10.65 SDD365 pKa = 3.62 RR366 pKa = 11.84 NADD369 pKa = 3.4 NTASSTRR376 pKa = 11.84 PRR378 pKa = 11.84 QQPP381 pKa = 2.92
Molecular weight: 45.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.167
IPC2_protein 9.428
IPC_protein 9.399
Toseland 10.028
ProMoST 9.721
Dawson 10.233
Bjellqvist 9.911
Wikipedia 10.409
Rodwell 10.687
Grimsley 10.306
Solomon 10.248
Lehninger 10.218
Nozaki 10.028
DTASelect 9.897
Thurlkill 10.087
EMBOSS 10.423
Sillero 10.145
Patrickios 10.131
IPC_peptide 10.248
IPC2_peptide 8.536
IPC2.peptide.svr19 8.422
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
9576
47
882
199.5
22.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.91 ± 0.697
1.138 ± 0.147
5.994 ± 0.233
7.498 ± 0.466
2.955 ± 0.22
6.381 ± 0.346
1.431 ± 0.239
6.422 ± 0.35
7.268 ± 0.434
8.229 ± 0.361
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.318 ± 0.117
4.971 ± 0.282
2.642 ± 0.179
4.522 ± 0.211
4.313 ± 0.417
4.845 ± 0.252
6.704 ± 0.384
6.683 ± 0.326
1.117 ± 0.146
4.657 ± 0.422
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here