Arthrobacter globiformis NBRC 12137
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4524 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H0QP97|H0QP97_ARTGO DUF853 domain-containing protein OS=Arthrobacter globiformis NBRC 12137 OX=1077972 GN=ARGLB_073_01700 PE=4 SV=1
MM1 pKa = 7.45 TEE3 pKa = 3.79 NSEE6 pKa = 4.29 SGDD9 pKa = 3.18 AGKK12 pKa = 7.91 THH14 pKa = 6.5 NSSRR18 pKa = 11.84 NPALEE23 pKa = 4.49 GGGGQYY29 pKa = 10.48 VEE31 pKa = 4.85 GDD33 pKa = 3.48 YY34 pKa = 11.64 GDD36 pKa = 4.51 AGVAGQEE43 pKa = 4.33 SAAEE47 pKa = 4.07 PKK49 pKa = 10.76 GEE51 pKa = 4.27 YY52 pKa = 9.43 PAGDD56 pKa = 3.68 YY57 pKa = 11.44 GDD59 pKa = 4.58 AGTVEE64 pKa = 4.47 PSAEE68 pKa = 4.18 VEE70 pKa = 3.89 EE71 pKa = 4.98 GEE73 pKa = 4.61 YY74 pKa = 10.62 PEE76 pKa = 5.45 GDD78 pKa = 3.45 YY79 pKa = 11.56 GRR81 pKa = 11.84 AGTVGQEE88 pKa = 4.1 LPGEE92 pKa = 4.31 EE93 pKa = 3.93 EE94 pKa = 4.21 GEE96 pKa = 4.34 YY97 pKa = 10.64 PEE99 pKa = 5.34 GDD101 pKa = 3.29 YY102 pKa = 11.3 GAAGTAPEE110 pKa = 4.7 GGAAEE115 pKa = 4.19 DD116 pKa = 5.07 LKK118 pKa = 11.51 DD119 pKa = 3.89 EE120 pKa = 5.3 LIGGQVNAPRR130 pKa = 11.84 NDD132 pKa = 3.97 DD133 pKa = 3.9 GGTSPGGG140 pKa = 3.3
Molecular weight: 13.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.694
IPC_protein 3.63
Toseland 3.452
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.49
Rodwell 3.465
Grimsley 3.363
Solomon 3.579
Lehninger 3.528
Nozaki 3.719
DTASelect 3.846
Thurlkill 3.49
EMBOSS 3.503
Sillero 3.745
Patrickios 0.896
IPC_peptide 3.579
IPC2_peptide 3.732
IPC2.peptide.svr19 3.698
Protein with the highest isoelectric point:
>tr|H0QKL2|H0QKL2_ARTGO Putative glycosyltransferase OS=Arthrobacter globiformis NBRC 12137 OX=1077972 GN=ARGLB_037_00620 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.97 KK16 pKa = 9.33 HH17 pKa = 4.25 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.68 GRR40 pKa = 11.84 TEE42 pKa = 4.13 LSAA45 pKa = 4.86
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.44
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.112
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4524
0
4524
1468277
30
2029
324.6
34.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.233 ± 0.047
0.621 ± 0.01
5.547 ± 0.029
5.55 ± 0.032
3.237 ± 0.029
9.309 ± 0.03
2.071 ± 0.018
4.301 ± 0.03
2.754 ± 0.028
10.189 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.958 ± 0.015
2.471 ± 0.017
5.542 ± 0.031
3.112 ± 0.02
6.509 ± 0.034
5.909 ± 0.024
5.889 ± 0.03
8.296 ± 0.032
1.421 ± 0.015
2.082 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here