Tepidimicrobium xylanilyticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Tissierellaceae; Tepidimicrobium

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2998 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H2VEG2|A0A1H2VEG2_9FIRM Phosphocarrier protein HPr OS=Tepidimicrobium xylanilyticum OX=1123352 GN=SAMN05660923_01091 PE=4 SV=1
MM1 pKa = 7.78DD2 pKa = 5.64RR3 pKa = 11.84YY4 pKa = 10.49VCEE7 pKa = 4.13PCGYY11 pKa = 10.67VYY13 pKa = 10.63DD14 pKa = 4.6PEE16 pKa = 5.92EE17 pKa = 4.62GDD19 pKa = 3.56PDD21 pKa = 3.76NGIEE25 pKa = 4.18PGTSFEE31 pKa = 4.6EE32 pKa = 5.31LPDD35 pKa = 3.85DD36 pKa = 4.22WVCPVCGATKK46 pKa = 10.84DD47 pKa = 3.54MFVKK51 pKa = 10.76EE52 pKa = 4.14EE53 pKa = 3.86

Molecular weight: 5.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3AUZ7|A0A1H3AUZ7_9FIRM NAD(P)-dependent iron-only hydrogenase diaphorase component iron-sulfur protein OS=Tepidimicrobium xylanilyticum OX=1123352 GN=SAMN05660923_02142 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.51QPKK8 pKa = 8.92RR9 pKa = 11.84RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.47EE15 pKa = 3.38HH16 pKa = 6.23GFRR19 pKa = 11.84KK20 pKa = 9.78RR21 pKa = 11.84MKK23 pKa = 8.75TKK25 pKa = 10.09SGRR28 pKa = 11.84NVIRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.84GRR39 pKa = 11.84VRR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2998

0

2998

872824

39

2659

291.1

32.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.579 ± 0.048

0.887 ± 0.018

5.59 ± 0.033

7.977 ± 0.053

4.241 ± 0.036

6.903 ± 0.048

1.432 ± 0.016

10.324 ± 0.043

8.263 ± 0.048

9.5 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.646 ± 0.022

5.491 ± 0.037

3.154 ± 0.026

2.294 ± 0.02

4.134 ± 0.027

5.576 ± 0.032

4.715 ± 0.024

6.463 ± 0.038

0.7 ± 0.015

4.127 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski