Paraglaciecola sp. MB-3u-78

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Paraglaciecola; unclassified Paraglaciecola

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5173 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N1D108|A0A2N1D108_9ALTE Toxin OS=Paraglaciecola sp. MB-3u-78 OX=2058332 GN=CXF95_26090 PE=3 SV=1
DDD2 pKa = 4.39ASVQLGSNSFRR13 pKa = 11.84LYYY16 pKa = 9.67DD17 pKa = 3.49QTRR20 pKa = 11.84FSVPSSFSLSADDD33 pKa = 2.81MTLTLIPDDD42 pKa = 4.26LLLANRR48 pKa = 11.84RR49 pKa = 11.84YYY51 pKa = 8.61YY52 pKa = 9.98YYY54 pKa = 10.52GYYY57 pKa = 10.32PYYY60 pKa = 10.69YYY62 pKa = 11.19DD63 pKa = 3.36ANNFIALNSFIQFVTGEEE81 pKa = 4.15TDDD84 pKa = 3.76DD85 pKa = 3.59APLLVNTNILNNALEEE101 pKa = 4.41PVNARR106 pKa = 11.84VVLTLNEEE114 pKa = 4.14LGSNCLTQLLITDDD128 pKa = 4.49VDDD131 pKa = 3.46DD132 pKa = 3.94AFASVLSSDDD142 pKa = 3.86RR143 pKa = 11.84TLTLTPSEEE152 pKa = 4.18LAANTSYYY160 pKa = 10.58VEEE163 pKa = 4.55TGLCDDD169 pKa = 3.4YY170 pKa = 11.15GNGLSGQALSFTTSTTGASDDD191 pKa = 3.29VRR193 pKa = 11.84PVLQSVVPANNAVGILVDDD212 pKa = 3.79NVVITFDDD220 pKa = 3.45EE221 pKa = 5.06ISLRR225 pKa = 11.84SSVLFYYY232 pKa = 11.34SDDD235 pKa = 3.3NQRR238 pKa = 11.84VLGSVDDD245 pKa = 3.13TDDD248 pKa = 3.77VLTFTPDDD256 pKa = 2.83EE257 pKa = 4.21L

Molecular weight: 27.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N1D8J0|A0A2N1D8J0_9ALTE Cytochrome c5 family protein OS=Paraglaciecola sp. MB-3u-78 OX=2058332 GN=CXF95_15505 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84KK29 pKa = 9.48VIANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5173

0

5173

1676260

33

2771

324.0

36.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.143 ± 0.034

1.042 ± 0.013

5.708 ± 0.031

5.74 ± 0.03

4.323 ± 0.022

6.671 ± 0.037

2.221 ± 0.019

6.786 ± 0.026

5.656 ± 0.031

10.361 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.487 ± 0.018

4.77 ± 0.028

3.807 ± 0.019

4.751 ± 0.032

4.126 ± 0.025

6.885 ± 0.028

5.481 ± 0.026

6.704 ± 0.026

1.239 ± 0.013

3.099 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski