Amino acid dipepetide frequency for Paraglaciecola sp. MB-3u-78

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.025AlaAla: 7.025 ± 0.092	0.917AlaCys: 0.917 ± 0.023	4.674AlaAsp: 4.674 ± 0.065	4.75AlaGlu: 4.75 ± 0.066	3.371AlaPhe: 3.371 ± 0.047	5.649AlaGly: 5.649 ± 0.072	1.557AlaHis: 1.557 ± 0.036	5.763AlaIle: 5.763 ± 0.067	5.014AlaLys: 5.014 ± 0.063	8.845AlaLeu: 8.845 ± 0.09	2.272AlaMet: 2.272 ± 0.044	3.859AlaAsn: 3.859 ± 0.055	2.649AlaPro: 2.649 ± 0.037	3.624AlaGln: 3.624 ± 0.055	3.084AlaArg: 3.084 ± 0.052	5.321AlaSer: 5.321 ± 0.062	4.44AlaThr: 4.44 ± 0.055	5.337AlaVal: 5.337 ± 0.066	0.87AlaTrp: 0.87 ± 0.025	2.41AlaTyr: 2.41 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	0.813CysAla: 0.813 ± 0.022	0.169CysCys: 0.169 ± 0.012	0.622CysAsp: 0.622 ± 0.022	0.586CysGlu: 0.586 ± 0.021	0.478CysPhe: 0.478 ± 0.018	0.826CysGly: 0.826 ± 0.026	0.355CysHis: 0.355 ± 0.022	0.675CysIle: 0.675 ± 0.022	0.508CysLys: 0.508 ± 0.019	1.118CysLeu: 1.118 ± 0.028	0.234CysMet: 0.234 ± 0.011	0.397CysAsn: 0.397 ± 0.016	0.477CysPro: 0.477 ± 0.018	0.491CysGln: 0.491 ± 0.018	0.415CysArg: 0.415 ± 0.018	0.718CysSer: 0.718 ± 0.025	0.434CysThr: 0.434 ± 0.017	0.65CysVal: 0.65 ± 0.02	0.125CysTrp: 0.125 ± 0.008	0.325CysTyr: 0.325 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.406AspAla: 4.406 ± 0.057	0.579AspCys: 0.579 ± 0.023	3.307AspAsp: 3.307 ± 0.059	3.61AspGlu: 3.61 ± 0.046	2.733AspPhe: 2.733 ± 0.043	4.111AspGly: 4.111 ± 0.097	1.025AspHis: 1.025 ± 0.025	4.413AspIle: 4.413 ± 0.055	3.591AspLys: 3.591 ± 0.05	5.407AspLeu: 5.407 ± 0.067	1.478AspMet: 1.478 ± 0.03	2.932AspAsn: 2.932 ± 0.054	2.16AspPro: 2.16 ± 0.043	1.907AspGln: 1.907 ± 0.037	1.987AspArg: 1.987 ± 0.034	3.435AspSer: 3.435 ± 0.052	3.004AspThr: 3.004 ± 0.049	3.968AspVal: 3.968 ± 0.053	0.916AspTrp: 0.916 ± 0.026	2.115AspTyr: 2.115 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	4.259GluAla: 4.259 ± 0.056	0.491GluCys: 0.491 ± 0.017	2.838GluAsp: 2.838 ± 0.049	3.022GluGlu: 3.022 ± 0.052	2.583GluPhe: 2.583 ± 0.038	3.181GluGly: 3.181 ± 0.048	1.52GluHis: 1.52 ± 0.031	3.823GluIle: 3.823 ± 0.05	3.668GluLys: 3.668 ± 0.052	6.604GluLeu: 6.604 ± 0.077	1.482GluMet: 1.482 ± 0.028	2.856GluAsn: 2.856 ± 0.048	1.84GluPro: 1.84 ± 0.039	3.845GluGln: 3.845 ± 0.062	2.618GluArg: 2.618 ± 0.043	3.446GluSer: 3.446 ± 0.052	2.963GluThr: 2.963 ± 0.04	3.817GluVal: 3.817 ± 0.049	0.641GluTrp: 0.641 ± 0.021	1.904GluTyr: 1.904 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	3.581PheAla: 3.581 ± 0.059	0.555PheCys: 0.555 ± 0.021	2.924PheAsp: 2.924 ± 0.045	2.729PheGlu: 2.729 ± 0.042	1.882PhePhe: 1.882 ± 0.035	3.124PheGly: 3.124 ± 0.046	0.875PheHis: 0.875 ± 0.025	3.042PheIle: 3.042 ± 0.046	2.235PheLys: 2.235 ± 0.04	3.632PheLeu: 3.632 ± 0.056	1.101PheMet: 1.101 ± 0.026	2.239PheAsn: 2.239 ± 0.039	1.45PhePro: 1.45 ± 0.032	1.243PheGln: 1.243 ± 0.026	1.565PheArg: 1.565 ± 0.03	3.729PheSer: 3.729 ± 0.052	2.466PheThr: 2.466 ± 0.042	2.855PheVal: 2.855 ± 0.045	0.518PheTrp: 0.518 ± 0.017	1.489PheTyr: 1.489 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	5.005GlyAla: 5.005 ± 0.076	0.818GlyCys: 0.818 ± 0.023	3.919GlyAsp: 3.919 ± 0.079	3.81GlyGlu: 3.81 ± 0.051	3.381GlyPhe: 3.381 ± 0.055	4.552GlyGly: 4.552 ± 0.078	1.518GlyHis: 1.518 ± 0.031	4.714GlyIle: 4.714 ± 0.062	4.014GlyLys: 4.014 ± 0.054	7.065GlyLeu: 7.065 ± 0.072	1.813GlyMet: 1.813 ± 0.033	2.945GlyAsn: 2.945 ± 0.057	1.656GlyPro: 1.656 ± 0.038	2.841GlyGln: 2.841 ± 0.045	2.72GlyArg: 2.72 ± 0.045	4.315GlySer: 4.315 ± 0.058	3.445GlyThr: 3.445 ± 0.063	4.832GlyVal: 4.832 ± 0.067	0.941GlyTrp: 0.941 ± 0.023	2.409GlyTyr: 2.409 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	1.575HisAla: 1.575 ± 0.032	0.345HisCys: 0.345 ± 0.015	1.061HisAsp: 1.061 ± 0.029	1.058HisGlu: 1.058 ± 0.026	1.139HisPhe: 1.139 ± 0.027	1.41HisGly: 1.41 ± 0.032	0.665HisHis: 0.665 ± 0.022	1.562HisIle: 1.562 ± 0.03	1.203HisLys: 1.203 ± 0.026	2.28HisLeu: 2.28 ± 0.039	0.502HisMet: 0.502 ± 0.02	1.033HisAsn: 1.033 ± 0.025	1.136HisPro: 1.136 ± 0.027	1.19HisGln: 1.19 ± 0.03	0.966HisArg: 0.966 ± 0.025	1.49HisSer: 1.49 ± 0.033	1.105HisThr: 1.105 ± 0.026	1.243HisVal: 1.243 ± 0.027	0.35HisTrp: 0.35 ± 0.015	0.897HisTyr: 0.897 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.117IleAla: 6.117 ± 0.075	0.739IleCys: 0.739 ± 0.024	4.623IleAsp: 4.623 ± 0.058	4.706IleGlu: 4.706 ± 0.058	2.57IlePhe: 2.57 ± 0.042	4.742IleGly: 4.742 ± 0.06	1.385IleHis: 1.385 ± 0.03	4.094IleIle: 4.094 ± 0.053	3.918IleLys: 3.918 ± 0.048	5.84IleLeu: 5.84 ± 0.077	1.394IleMet: 1.394 ± 0.025	3.646IleAsn: 3.646 ± 0.052	2.713IlePro: 2.713 ± 0.046	2.676IleGln: 2.676 ± 0.045	2.799IleArg: 2.799 ± 0.042	5.105IleSer: 5.105 ± 0.058	3.874IleThr: 3.874 ± 0.052	4.293IleVal: 4.293 ± 0.06	0.749IleTrp: 0.749 ± 0.023	1.878IleTyr: 1.878 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	4.647LysAla: 4.647 ± 0.063	0.381LysCys: 0.381 ± 0.015	2.85LysAsp: 2.85 ± 0.039	3.028LysGlu: 3.028 ± 0.049	1.844LysPhe: 1.844 ± 0.035	3.501LysGly: 3.501 ± 0.052	1.446LysHis: 1.446 ± 0.03	3.73LysIle: 3.73 ± 0.049	3.317LysLys: 3.317 ± 0.055	5.712LysLeu: 5.712 ± 0.065	1.518LysMet: 1.518 ± 0.033	2.818LysAsn: 2.818 ± 0.041	2.332LysPro: 2.332 ± 0.041	3.386LysGln: 3.386 ± 0.045	2.775LysArg: 2.775 ± 0.045	3.642LysSer: 3.642 ± 0.057	3.448LysThr: 3.448 ± 0.054	3.942LysVal: 3.942 ± 0.048	0.65LysTrp: 0.65 ± 0.018	1.595LysTyr: 1.595 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	9.343LeuAla: 9.343 ± 0.076	1.1LeuCys: 1.1 ± 0.028	5.828LeuAsp: 5.828 ± 0.062	5.65LeuGlu: 5.65 ± 0.065	4.329LeuPhe: 4.329 ± 0.057	6.921LeuGly: 6.921 ± 0.076	2.076LeuHis: 2.076 ± 0.034	6.779LeuIle: 6.779 ± 0.071	5.713LeuLys: 5.713 ± 0.065	10.681LeuLeu: 10.681 ± 0.118	2.584LeuMet: 2.584 ± 0.043	5.012LeuAsn: 5.012 ± 0.062	4.526LeuPro: 4.526 ± 0.055	4.122LeuGln: 4.122 ± 0.058	4.166LeuArg: 4.166 ± 0.052	8.014LeuSer: 8.014 ± 0.079	6.114LeuThr: 6.114 ± 0.06	6.811LeuVal: 6.811 ± 0.078	1.076LeuTrp: 1.076 ± 0.032	2.763LeuTyr: 2.763 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.256MetAla: 2.256 ± 0.037	0.22MetCys: 0.22 ± 0.011	1.224MetAsp: 1.224 ± 0.03	1.103MetGlu: 1.103 ± 0.026	0.999MetPhe: 0.999 ± 0.024	1.678MetGly: 1.678 ± 0.035	0.536MetHis: 0.536 ± 0.021	1.397MetIle: 1.397 ± 0.031	1.339MetLys: 1.339 ± 0.031	2.847MetLeu: 2.847 ± 0.042	0.655MetMet: 0.655 ± 0.019	1.088MetAsn: 1.088 ± 0.028	1.265MetPro: 1.265 ± 0.026	1.375MetGln: 1.375 ± 0.028	1.101MetArg: 1.101 ± 0.026	1.914MetSer: 1.914 ± 0.033	1.518MetThr: 1.518 ± 0.029	1.571MetVal: 1.571 ± 0.036	0.233MetTrp: 0.233 ± 0.014	0.548MetTyr: 0.548 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.575AsnAla: 3.575 ± 0.053	0.504AsnCys: 0.504 ± 0.019	2.599AsnAsp: 2.599 ± 0.047	2.572AsnGlu: 2.572 ± 0.042	1.878AsnPhe: 1.878 ± 0.035	3.203AsnGly: 3.203 ± 0.061	1.013AsnHis: 1.013 ± 0.024	3.706AsnIle: 3.706 ± 0.043	2.935AsnLys: 2.935 ± 0.045	4.441AsnLeu: 4.441 ± 0.063	1.154AsnMet: 1.154 ± 0.025	2.537AsnAsn: 2.537 ± 0.054	2.099AsnPro: 2.099 ± 0.036	2.47AsnGln: 2.47 ± 0.047	1.978AsnArg: 1.978 ± 0.035	3.036AsnSer: 3.036 ± 0.052	2.75AsnThr: 2.75 ± 0.048	2.975AsnVal: 2.975 ± 0.048	0.737AsnTrp: 0.737 ± 0.022	1.537AsnTyr: 1.537 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	2.828ProAla: 2.828 ± 0.044	0.31ProCys: 0.31 ± 0.013	2.468ProAsp: 2.468 ± 0.039	2.859ProGlu: 2.859 ± 0.052	1.719ProPhe: 1.719 ± 0.032	2.239ProGly: 2.239 ± 0.043	0.787ProHis: 0.787 ± 0.023	2.763ProIle: 2.763 ± 0.045	2.221ProLys: 2.221 ± 0.039	3.868ProLeu: 3.868 ± 0.053	0.937ProMet: 0.937 ± 0.024	1.959ProAsn: 1.959 ± 0.036	1.19ProPro: 1.19 ± 0.03	1.64ProGln: 1.64 ± 0.031	1.26ProArg: 1.26 ± 0.029	2.603ProSer: 2.603 ± 0.04	1.984ProThr: 1.984 ± 0.033	2.785ProVal: 2.785 ± 0.048	0.487ProTrp: 0.487 ± 0.018	1.16ProTyr: 1.16 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	4.303GlnAla: 4.303 ± 0.052	0.395GlnCys: 0.395 ± 0.016	2.235GlnAsp: 2.235 ± 0.037	2.28GlnGlu: 2.28 ± 0.046	1.834GlnPhe: 1.834 ± 0.033	2.972GlnGly: 2.972 ± 0.037	1.288GlnHis: 1.288 ± 0.03	2.998GlnIle: 2.998 ± 0.046	2.516GlnLys: 2.516 ± 0.044	5.167GlnLeu: 5.167 ± 0.081	1.049GlnMet: 1.049 ± 0.024	2.052GlnAsn: 2.052 ± 0.042	1.691GlnPro: 1.691 ± 0.033	3.578GlnGln: 3.578 ± 0.082	2.181GlnArg: 2.181 ± 0.044	3.152GlnSer: 3.152 ± 0.048	2.627GlnThr: 2.627 ± 0.039	3.134GlnVal: 3.134 ± 0.045	0.64GlnTrp: 0.64 ± 0.022	1.422GlnTyr: 1.422 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	3.042ArgAla: 3.042 ± 0.038	0.406ArgCys: 0.406 ± 0.017	2.209ArgAsp: 2.209 ± 0.037	2.408ArgGlu: 2.408 ± 0.037	2.077ArgPhe: 2.077 ± 0.036	2.356ArgGly: 2.356 ± 0.038	0.967ArgHis: 0.967 ± 0.025	2.883ArgIle: 2.883 ± 0.043	2.327ArgLys: 2.327 ± 0.042	4.701ArgLeu: 4.701 ± 0.062	1.039ArgMet: 1.039 ± 0.026	1.888ArgAsn: 1.888 ± 0.035	1.521ArgPro: 1.521 ± 0.032	2.071ArgGln: 2.071 ± 0.036	1.965ArgArg: 1.965 ± 0.039	2.462ArgSer: 2.462 ± 0.04	1.969ArgThr: 1.969 ± 0.035	2.802ArgVal: 2.802 ± 0.05	0.581ArgTrp: 0.581 ± 0.019	1.584ArgTyr: 1.584 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	5.406SerAla: 5.406 ± 0.063	0.762SerCys: 0.762 ± 0.023	3.977SerAsp: 3.977 ± 0.061	4.044SerGlu: 4.044 ± 0.049	2.994SerPhe: 2.994 ± 0.04	5.01SerGly: 5.01 ± 0.068	1.589SerHis: 1.589 ± 0.03	4.747SerIle: 4.747 ± 0.059	3.616SerLys: 3.616 ± 0.05	7.31SerLeu: 7.31 ± 0.073	1.706SerMet: 1.706 ± 0.034	3.038SerAsn: 3.038 ± 0.045	2.526SerPro: 2.526 ± 0.04	3.224SerGln: 3.224 ± 0.043	2.828SerArg: 2.828 ± 0.043	4.816SerSer: 4.816 ± 0.073	3.556SerThr: 3.556 ± 0.055	4.704SerVal: 4.704 ± 0.06	0.86SerTrp: 0.86 ± 0.023	2.137SerTyr: 2.137 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	4.389ThrAla: 4.389 ± 0.055	0.51ThrCys: 0.51 ± 0.02	3.358ThrAsp: 3.358 ± 0.06	3.077ThrGlu: 3.077 ± 0.044	2.3ThrPhe: 2.3 ± 0.036	4.116ThrGly: 4.116 ± 0.058	1.267ThrHis: 1.267 ± 0.027	3.596ThrIle: 3.596 ± 0.051	2.708ThrLys: 2.708 ± 0.05	6.229ThrLeu: 6.229 ± 0.071	1.175ThrMet: 1.175 ± 0.029	2.33ThrAsn: 2.33 ± 0.044	2.56ThrPro: 2.56 ± 0.037	2.6ThrGln: 2.6 ± 0.039	2.054ThrArg: 2.054 ± 0.035	3.711ThrSer: 3.711 ± 0.057	2.982ThrThr: 2.982 ± 0.054	3.645ThrVal: 3.645 ± 0.053	0.629ThrTrp: 0.629 ± 0.021	1.573ThrTyr: 1.573 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	5.615ValAla: 5.615 ± 0.06	0.71ValCys: 0.71 ± 0.022	4.067ValAsp: 4.067 ± 0.051	4.008ValGlu: 4.008 ± 0.055	2.967ValPhe: 2.967 ± 0.047	4.359ValGly: 4.359 ± 0.056	1.207ValHis: 1.207 ± 0.03	4.691ValIle: 4.691 ± 0.059	3.731ValLys: 3.731 ± 0.047	6.703ValLeu: 6.703 ± 0.076	1.8ValMet: 1.8 ± 0.035	3.403ValAsn: 3.403 ± 0.047	2.48ValPro: 2.48 ± 0.047	2.263ValGln: 2.263 ± 0.041	2.581ValArg: 2.581 ± 0.041	4.945ValSer: 4.945 ± 0.067	4.088ValThr: 4.088 ± 0.06	4.899ValVal: 4.899 ± 0.07	0.737ValTrp: 0.737 ± 0.024	1.783ValTyr: 1.783 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	0.824TrpAla: 0.824 ± 0.023	0.128TrpCys: 0.128 ± 0.009	0.619TrpAsp: 0.619 ± 0.018	0.536TrpGlu: 0.536 ± 0.018	0.563TrpPhe: 0.563 ± 0.019	0.741TrpGly: 0.741 ± 0.025	0.379TrpHis: 0.379 ± 0.017	0.703TrpIle: 0.703 ± 0.021	0.511TrpLys: 0.511 ± 0.017	1.682TrpLeu: 1.682 ± 0.041	0.319TrpMet: 0.319 ± 0.015	0.478TrpAsn: 0.478 ± 0.016	0.473TrpPro: 0.473 ± 0.016	1.037TrpGln: 1.037 ± 0.03	0.652TrpArg: 0.652 ± 0.021	0.766TrpSer: 0.766 ± 0.025	0.533TrpThr: 0.533 ± 0.02	0.892TrpVal: 0.892 ± 0.025	0.178TrpTrp: 0.178 ± 0.01	0.37TrpTyr: 0.37 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.422TyrAla: 2.422 ± 0.045	0.377TyrCys: 0.377 ± 0.017	1.681TyrAsp: 1.681 ± 0.037	1.566TyrGlu: 1.566 ± 0.034	1.594TyrPhe: 1.594 ± 0.032	2.02TyrGly: 2.02 ± 0.037	0.754TyrHis: 0.754 ± 0.021	1.782TyrIle: 1.782 ± 0.037	1.477TyrLys: 1.477 ± 0.031	3.473TyrLeu: 3.473 ± 0.048	0.654TyrMet: 0.654 ± 0.02	1.188TyrAsn: 1.188 ± 0.03	1.324TyrPro: 1.324 ± 0.028	1.931TyrGln: 1.931 ± 0.041	1.562TyrArg: 1.562 ± 0.031	2.231TyrSer: 2.231 ± 0.037	1.51TyrThr: 1.51 ± 0.03	1.883TyrVal: 1.883 ± 0.037	0.468TyrTrp: 0.468 ± 0.017	1.097TyrTyr: 1.097 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5173 proteins (1676260 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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