Vibrio tapetis subsp. tapetis
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5392 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N8ZDF1|A0A2N8ZDF1_9VIBR Uncharacterized protein OS=Vibrio tapetis subsp. tapetis OX=1671868 GN=VTAP4600_A1937 PE=4 SV=1
MM1 pKa = 7.81 WDD3 pKa = 5.41 DD4 pKa = 3.69 PLLWAILPIYY14 pKa = 10.58 ASLFVYY20 pKa = 9.84 DD21 pKa = 3.52 WWDD24 pKa = 3.27 ALIAASGLEE33 pKa = 4.07 TQDD36 pKa = 3.41 EE37 pKa = 4.98 VKK39 pKa = 10.61 SEE41 pKa = 4.13 HH42 pKa = 6.44
Molecular weight: 4.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 3.77
IPC_protein 3.605
Toseland 3.427
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.617
Rodwell 3.465
Grimsley 3.35
Solomon 3.567
Lehninger 3.528
Nozaki 3.795
DTASelect 3.961
Thurlkill 3.528
EMBOSS 3.63
Sillero 3.745
Patrickios 1.863
IPC_peptide 3.567
IPC2_peptide 3.706
IPC2.peptide.svr19 3.723
Protein with the highest isoelectric point:
>tr|A0A2N8ZHE1|A0A2N8ZHE1_9VIBR Putative 2Fe-2S cluster-containing dioxygenase subunit OS=Vibrio tapetis subsp. tapetis OX=1671868 GN=yeaW PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 8.98 RR4 pKa = 11.84 TFQPSVLKK12 pKa = 10.42 RR13 pKa = 11.84 KK14 pKa = 9.36 RR15 pKa = 11.84 SHH17 pKa = 6.15 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.44 NGRR29 pKa = 11.84 ATINARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.74 GRR40 pKa = 11.84 KK41 pKa = 8.91 RR42 pKa = 11.84 LSKK45 pKa = 10.84
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5392
0
5392
1632385
15
3843
302.7
33.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.224 ± 0.034
1.079 ± 0.012
5.483 ± 0.039
6.231 ± 0.036
4.159 ± 0.024
6.613 ± 0.036
2.297 ± 0.022
6.374 ± 0.026
5.541 ± 0.028
10.319 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.694 ± 0.019
4.438 ± 0.024
3.737 ± 0.019
4.567 ± 0.03
4.435 ± 0.031
7.064 ± 0.028
5.495 ± 0.042
6.951 ± 0.029
1.25 ± 0.014
3.05 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here