Siphoviridae sp. ctjeG17

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WAH0|A0A5Q2WAH0_9CAUD DNA primase/helicase OS=Siphoviridae sp. ctjeG17 OX=2656721 PE=4 SV=1
MM1 pKa = 7.67GAMDD5 pKa = 4.18YY6 pKa = 11.23LKK8 pKa = 10.75FILVVQAFFAFSITMLSYY26 pKa = 11.19ALPDD30 pKa = 3.35TAVNHH35 pKa = 6.11IAIFTDD41 pKa = 3.29QSGNLDD47 pKa = 3.89LASVNQDD54 pKa = 3.23FKK56 pKa = 11.53QSLSEE61 pKa = 3.85QRR63 pKa = 11.84NIPIIDD69 pKa = 3.31MGALVFYY76 pKa = 10.41SGNFILDD83 pKa = 3.69LMLNFAFAVPQMIAMLINGFSLIFGGIDD111 pKa = 3.2TTILAMIQTFLSLLIGTLYY130 pKa = 10.05MVGLIQTVTNIRR142 pKa = 11.84SQGGLQQ148 pKa = 3.12

Molecular weight: 16.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W9W0|A0A5Q2W9W0_9CAUD Uncharacterized protein OS=Siphoviridae sp. ctjeG17 OX=2656721 PE=4 SV=1
MM1 pKa = 7.6SKK3 pKa = 8.74TALIKK8 pKa = 10.69SIMKK12 pKa = 9.11QRR14 pKa = 11.84RR15 pKa = 11.84AIKK18 pKa = 10.19KK19 pKa = 9.54IMRR22 pKa = 11.84SMNGHH27 pKa = 6.87KK28 pKa = 10.1KK29 pKa = 8.8PKK31 pKa = 10.04RR32 pKa = 11.84MPARR36 pKa = 11.84LRR38 pKa = 11.84AQLYY42 pKa = 9.77RR43 pKa = 11.84GHH45 pKa = 7.11

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

16986

30

1411

178.8

20.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.652 ± 0.322

1.578 ± 0.188

4.722 ± 0.25

5.228 ± 0.442

5.234 ± 0.237

6.028 ± 0.312

1.195 ± 0.113

8.401 ± 0.317

6.499 ± 0.56

8.348 ± 0.332

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.367 ± 0.243

7.294 ± 0.761

2.826 ± 0.224

4.056 ± 0.404

3.709 ± 0.261

7.159 ± 0.4

7.312 ± 0.485

6.346 ± 0.3

1.254 ± 0.115

4.792 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski